Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,920,827 T→C 5.0% V202A (GTG→GCG)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,920,8270TC 5.0% 240.8 / 2.7 79V202A (GTG→GCG) rsmF16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (40/35);  new base C (2/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

CGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAG  >  NC_000913/1920682‑1920975
                                                                                                                                                 |                                                                                                                                                    
cGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGGTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgtggt                                                                                                                                                   <  5:244922/149‑1 (MQ=255)
 gggTAAAAGTGTTACATGCCAATATGAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCgtggtg                                                                                                                                                  >  4:30262/1‑149 (MQ=255)
       aaGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTa                                                                                                                                              >  8:166220/1‑147 (MQ=255)
            ttACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATcc                                                                                                                                       <  7:215645/149‑1 (MQ=255)
               cATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGa                                                                                                                                    >  8:83400/1‑149 (MQ=255)
                aTGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTGTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGAt                                                                                                                                   <  8:248187/149‑1 (MQ=255)
                    cAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATgcgc                                                                                                                               >  8:42513/1‑149 (MQ=255)
                         tCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaa                                                                                                                           <  7:337722/148‑1 (MQ=255)
                              cGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACtt                                                                                                                     <  8:281666/149‑2 (MQ=255)
                              cGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTg                                                                                                                     <  8:113240/149‑1 (MQ=255)
                               gCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTgg                                                                                                                    >  4:7212/1‑149 (MQ=255)
                                   tgGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCAc                                                                                                                <  7:214318/149‑1 (MQ=255)
                                     gTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCa                                                                                                              <  3:30262/149‑1 (MQ=255)
                                        tCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGaa                                                                                                           <  1:44565/149‑1 (MQ=255)
                                          aGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGaaa                                                                                                          <  5:178226/148‑1 (MQ=255)
                                           gTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGTCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAg                                                                                                         >  5:262997/1‑148 (MQ=255)
                                           gTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaa                                                                                                                           >  4:246644/1‑130 (MQ=255)
                                           gTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTaaa                                                                                                                           <  3:246644/130‑1 (MQ=255)
                                                gTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAAt                                                                                                     >  8:2911/1‑147 (MQ=255)
                                                 ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCa                                                                                                   <  7:83400/148‑1 (MQ=255)
                                                 ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAg                                                                                                  >  5:51767/1‑149 (MQ=255)
                                                 ttGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAg                                                                                                  >  1:217617/1‑149 (MQ=255)
                                                   gcgcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAgg                                                                                                 <  7:166220/148‑1 (MQ=255)
                                                     gcTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGaaa                                                                                              <  8:84035/149‑1 (MQ=255)
                                                       tCACACATTTTGATGGCCGCGTGTTTGGTGCGTCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATGCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGaaa                                                                                              <  3:117396/147‑1 (MQ=255)
                                                         acacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCg                                                                                           >  5:342753/1‑148 (MQ=255)
                                                          cacaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCa                                                                                         <  2:333706/149‑1 (MQ=255)
                                                           acaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAg                                                                                        <  7:42513/149‑1 (MQ=255)
                                                           acaTTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAg                                                                                        >  5:8635/1‑149 (MQ=255)
                                                              ttttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTa                                                                                     >  7:252387/1‑149 (MQ=255)
                                                              ttttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTa                                                                                     >  6:173347/1‑149 (MQ=255)
                                                                ttGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTaca                                                                                   >  7:306840/1‑149 (MQ=255)
                                                                  gATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTacac                                                                                  <  7:2911/148‑1 (MQ=255)
                                                                      gCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATAGCAGCTACACAACgg                                                                             <  2:213756/149‑1 (MQ=255)
                                                                       ccGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACggg                                                                            <  3:7212/149‑1 (MQ=255)
                                                                         gcgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAg                                                                          >  4:110964/1‑149 (MQ=255)
                                                                          cgTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGc                                                                         >  4:111687/1‑149 (MQ=255)
                                                                                 ggTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGa                                                                  >  4:328602/1‑149 (MQ=255)
                                                                                  gTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGa                                                                  >  8:191456/1‑148 (MQ=255)
                                                                                  gTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGAc                                                                 <  2:217617/149‑1 (MQ=255)
                                                                                  gTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGAc                                                                 <  6:51767/149‑1 (MQ=255)
                                                                                    gCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGAAGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAg                                                               >  6:3084/1‑149 (MQ=255)
                                                                                         aGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCGCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCt                                                          <  2:9145/149‑1 (MQ=255)
                                                                                           tGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCAc                                                                                                                >  1:116953/1‑93 (MQ=255)
                                                                                           tGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCAc                                                                                                                <  2:116953/93‑1 (MQ=255)
                                                                                             ccAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCa                                                      >  2:258871/1‑149 (MQ=255)
                                                                                             ccAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCa                                                      <  5:173347/149‑1 (MQ=255)
                                                                                              cAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCAt                                                     >  7:323505/1‑149 (MQ=255)
                                                                                              cAGAAATGTTCGATGACATTTTGCTGGACGCTCCATGCTCAGGCGAAGGAGTGGTGCGGAAAGATCCCGATGCGCTAAAAATATTGTCACCATAAAGCAATCAGGAAAACGCAGCTACACAACGGGAGATGATCGACAGCGCATTTCAt                                                     >  5:225699/1‑149 (MQ=255)
                                                                                               aGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATg                                                    <  8:38724/149‑1 (MQ=255)
                                                                                                gAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGt                                                                                                                   <  3:54908/85‑1 (MQ=255)
                                                                                                gAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGt                                                                                                                   >  4:54908/1‑85 (MQ=255)
                                                                                                 aaaTGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGc                                                                         <  5:337667/126‑1 (MQ=255)
                                                                                                 aaaTGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGc                                                                         >  6:337667/1‑126 (MQ=255)
                                                                                                        cGATGCCCTTTTGCTGGACTCTCCCTGCTCTGGCGAAGGCGTGGTTCCTAAAGATCCCGATGCGATAAAAAACTGGTCACCAGAAAGCAATCAGCAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCAATTCATGCAGTACggc                                           >  4:288739/1‑147 (MQ=255)
                                                                                                         gATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGCGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTcc                                          >  8:91105/1‑149 (MQ=255)
                                                                                                         gATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGCGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTcc                                          >  4:38385/1‑149 (MQ=255)
                                                                                                          aTGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCt                                         >  8:143456/1‑149 (MQ=255)
                                                                                                           tGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtg                                        <  8:323505/149‑1 (MQ=255)
                                                                                                           tGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtg                                        <  8:129571/149‑1 (MQ=255)
                                                                                                            gCCATTTTGCTGGACGCTCCCTGCTCTGGCGTAGGCGTGGTGCGTAAAGATCCAGATGCTCTAAAAAACGGGTACCCAGAAAGAAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGCCAGCGCCTTTCATGCATTACGTCCtgg                                       >  7:239759/1‑149 (MQ=255)
                                                                                                            gCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCtgg                                       >  8:36510/1‑149 (MQ=255)
                                                                                                                ttttGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTggtggt                                   >  1:343985/1‑149 (MQ=255)
                                                                                                                    gCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCTTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGc                               >  5:309041/1‑149 (MQ=255)
                                                                                                                      tGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTg                             >  3:216981/1‑149 (MQ=255)
                                                                                                                      tGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTg                             >  4:197204/1‑149 (MQ=255)
                                                                                                                      tGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTg                             >  8:13428/1‑149 (MQ=255)
                                                                                                                        gACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGt                           >  1:199529/1‑149 (MQ=255)
                                                                                                                        gACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCGAAAAAACTGGTCCCCAGACAGCACTCAGGACATCGCCGCTACACACCGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTggg                           <  7:241321/149‑2 (MQ=255)
                                                                                                                           gCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTa                        >  6:43146/1‑149 (MQ=255)
                                                                                                                           gCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTa                        <  7:143456/149‑1 (MQ=255)
                                                                                                                           gCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTa                        <  1:258871/149‑1 (MQ=255)
                                                                                                                             tCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACt                      >  4:61472/1‑149 (MQ=255)
                                                                                                                               ccTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCt                                                                                      >  5:57181/1‑83 (MQ=255)
                                                                                                                               ccTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCt                                                                                      <  6:57181/83‑1 (MQ=255)
                                                                                                                               ccTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTc                     <  3:61472/148‑1 (MQ=255)
                                                                                                                                       ggCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAg                                                                                                         >  8:26740/1‑56 (MQ=255)
                                                                                                                                       ggCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAg                                                                                                         <  7:26740/56‑1 (MQ=255)
                                                                                                                                        gCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTAcc           >  5:219316/1‑149 (MQ=255)
                                                                                                                                           aaGGCGCGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTa        <  7:91105/149‑1 (MQ=255)
                                                                                                                                           aaGGCGCGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTa        <  3:38385/149‑1 (MQ=255)
                                                                                                                                                gtggtgCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCa   <  3:197204/149‑1 (MQ=255)
                                                                                                                                                gtggcgCGGAAACATCCCGACGCGCTAAAAAACTGGCCACCAGAAAGCACTCAGGAAATCGCAGCTACACACCGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCa   <  1:253080/149‑1 (MQ=255)
                                                                                                                                                 tggtgCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAg  <  4:154622/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGGTAAAAGTGTTACATGCCAATATCAGCCGCTGTGGTATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAATGTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGTCACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGTACGCTGGTTTACTCGACCTGTACCTTAAACCAG  >  NC_000913/1920682‑1920975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: