Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,331,019 C→T 5.3% D246N (GAT→AAT)  obgE ← GTPase involved in cell partioning and DNA repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,331,0190CT 5.3% 238.4 / 2.2 75D246N (GAT→AAT) obgEGTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base C (30/40);  new base T (2/2);  total (32/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

TCTTCGGCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGA  >  NC_000913/3330871‑3331164
                                                                                                                                                    |                                                                                                                                                 
tctTCGGCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGatcgatatc                                                                                                                                                   >  7:305002/1‑149 (MQ=255)
    cGGCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGatcgatatcgat                                                                                                                                                <  6:151712/148‑1 (MQ=255)
    cGGCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGatcgatatcgat                                                                                                                                                <  7:155442/148‑1 (MQ=255)
     ggCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAACAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATg                                                                                                                                               <  7:81803/148‑1 (MQ=255)
      gCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAg                                                                                                                                             >  3:232087/1‑149 (MQ=255)
       cTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAgg                                                                                                                                            <  4:232087/149‑1 (MQ=255)
        ttCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGt                                                                                                                                           >  2:105280/1‑149 (MQ=255)
             ccTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTGAATCGGATCGATATCGATGAGGTGCaa                                                                                                                                       <  2:84488/148‑1 (MQ=255)
             ccTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCaa                                                                                                                                       >  1:132570/1‑148 (MQ=255)
             ccTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCaa                                                                                                                                       >  8:350640/1‑148 (MQ=255)
              cTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATTGATGAGGTGCaa                                                                                                                                       >  4:246349/1‑147 (MQ=255)
               ttATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAg                                                                                                                                    >  3:242112/1‑149 (MQ=255)
                    cagcagATCGATCTTGTTGAACACTAACCAACGCGGTGTCGTCGCCAGAGCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGAcgc                                                                                                                               <  1:53038/149‑1 (MQ=255)
                     agcagATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACTCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGAcgcg                                                                                                                              >  1:83351/1‑149 (MQ=255)
                     agcagATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGAcgcg                                                                                                                              >  2:139198/1‑149 (MQ=255)
                      gcagATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCgg                                                                                                                             <  4:242112/149‑1 (MQ=255)
                               tcTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTc                                                                                                                     >  6:21485/1‑148 (MQ=255)
                                 ttgttgAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAg                                                                                                                  >  8:215161/1‑149 (MQ=255)
                                   gttgAACACTAACCAACGCGGTTTCGTCGCCTGATCCTGGCTGTATTTTTCCAGCTGGCTGATAATAATACGCGCGTTTTCAACCGGATCGGGGCCGTCAAGCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAg                                                                                                                  <  2:129216/147‑1 (MQ=255)
                                   gttgAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCTACAGGACGCGGCAACGTTc                                                                                                                     <  3:198413/144‑1 (MQ=255)
                                   gttgAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCa                                                                                                                   <  1:114508/146‑1 (MQ=255)
                                    ttgAACACTAACCAACGCGGTTTCGTAGCCAGATCCTGGCTGTATTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGc                                                                                                              <  1:287779/149‑1 (MQ=255)
                                      gAACACTAACCAGCGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGAGGAGGTGCAACAGGACGGGGCAAAGTTCCAGGTGCt                                                                                                             >  5:108108/1‑149 (MQ=255)
                                        acacTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTc                                                                                                           <  5:132974/149‑1 (MQ=255)
                                          acTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAg                                                                                                         >  3:283319/1‑149 (MQ=255)
                                          acTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAg                                                                                                         >  8:270265/1‑149 (MQ=255)
                                            tAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAgg                                                                                                        >  6:238707/1‑148 (MQ=255)
                                                cAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGc                                                                                                    >  8:98582/1‑148 (MQ=255)
                                                 aaCGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCg                                                                                                   >  8:235864/1‑148 (MQ=255)
                                                  aagcgGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCCACCGGATCGGTGCCGTCAATCGGATCGATATCAATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGaa                                                                                                 <  1:104355/147‑1 (MQ=255)
                                                    gcgGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATg                                                                                               >  1:268968/1‑149 (MQ=255)
                                                     cgGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTAGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCTGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGc                                                                                              <  8:294462/149‑1 (MQ=255)
                                                      ggTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGcc                                                                                             <  7:350640/149‑1 (MQ=255)
                                                       gTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCTACCGGATCGGTGCCGTCAATCGGTTCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGcc                                                                                             >  3:299206/1‑148 (MQ=255)
                                                       gTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGccc                                                                                            <  8:182203/149‑1 (MQ=255)
                                                              cgCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTg                                                                                     >  6:228088/1‑149 (MQ=255)
                                                               gCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATTGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTgc                                                                                    >  6:270858/1‑149 (MQ=255)
                                                                ccAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTgcg                                                                                   >  5:228845/1‑149 (MQ=255)
                                                                 cAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTgcgc                                                                                  >  2:215550/1‑149 (MQ=255)
                                                                       cTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCg                                                                            <  7:270265/149‑1 (MQ=255)
                                                                           cTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAgc                                                                        <  5:228088/149‑1 (MQ=255)
                                                                                tttttCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATAGTATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGTTGCTTCAGGAAGCGaa                                                                                                 <  7:86484/119‑1 (MQ=255)
                                                                                tttttCCAGCTAGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATAGGATCGATATCGATGAGGAGCAACAGAACGCGGCAACGTTCCAGGTGCTTCAGGAAGAGaa                                                                                                 >  8:86484/1‑119 (MQ=255)
                                                                                     ccAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATTGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAAt                                                               <  3:246349/148‑1 (MQ=255)
                                                                                       aGCTCGCTGATAATAATACGCGCGTTTTCAACCGGCTCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCCCAGCGCCTTCAATCAg                                                            >  3:209444/1‑149 (MQ=255)
                                                                                       aGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAAAAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCCGCGCCTTCAATCAg                                                            >  3:204955/1‑149 (MQ=255)
                                                                                        gCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGt                                                           >  3:257818/1‑149 (MQ=255)
                                                                                         cTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTc                                                          <  7:98582/149‑1 (MQ=255)
                                                                                            gCTTATAATCCTACTCGCGTATTCAACCTGATCGGTGCCGTCACTCGTATCCATATAGATGAGGTGCCACAGGAAGCGGCAACTTTCCAGGTTCTTCAGGAAGCGAATGCCCAGACCTGCTCCTTCCGCAGCGCCTTCAATCAGTCCt                                                        <  6:108195/148‑1 (MQ=255)
                                                                                            gCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCt                                                        <  1:357023/148‑1 (MQ=255)
                                                                                            gCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTg                                                       <  3:225361/149‑1 (MQ=255)
                                                                                            gCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTg                                                       <  6:228845/149‑1 (MQ=255)
                                                                                            gCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTg                                                       >  2:226114/1‑149 (MQ=255)
                                                                                                 taataataCGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAata                                                  <  5:21485/149‑1 (MQ=255)
                                                                                                   ataataCGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAatat                                                 <  2:268968/148‑1 (MQ=255)
                                                                                                   ataataCGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATc                                                <  7:215161/149‑1 (MQ=255)
                                                                                                    taataCGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCg                                               <  2:244103/149‑1 (MQ=255)
                                                                                                       taCGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGca                                            >  1:96723/1‑149 (MQ=255)
                                                                                                         cgcgcgTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGcaac                                          <  6:49173/149‑1 (MQ=255)
                                                                                                         cgcgcgTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGcaac                                          <  2:209743/149‑1 (MQ=255)
                                                                                                          gcgcgTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGcaac                                          >  7:248494/1‑148 (MQ=255)
                                                                                                               ttttCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGaa                                    <  4:279952/149‑1 (MQ=255)
                                                                                                                   cAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGctc                                >  3:179108/1‑149 (MQ=255)
                                                                                                                       cGGATCGGTGCCGTCAATCGGAGCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCtttt                            <  1:78450/149‑1 (MQ=255)
                                                                                                                           tCGGTGCCGTCAATCGGATCGATATTGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGtt                        <  5:270858/149‑1 (MQ=255)
                                                                                                                           tCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGtt                        <  7:36569/149‑1 (MQ=255)
                                                                                                                             ggTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGATGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCGCTTTTCGTTGt                      <  2:83351/149‑1 (MQ=255)
                                                                                                                             ggTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTg                       <  2:347176/148‑1 (MQ=255)
                                                                                                                              gTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACg                                      <  2:34861/132‑1 (MQ=255)
                                                                                                                              gTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACg                                      >  1:34861/1‑132 (MQ=255)
                                                                                                                                  cGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCAtt                 <  8:248494/149‑1 (MQ=255)
                                                                                                                                   gTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCTGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTc                <  5:238707/149‑1 (MQ=255)
                                                                                                                                   gTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTc                <  4:179108/149‑1 (MQ=255)
                                                                                                                                      aaTCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTa             <  1:139198/149‑1 (MQ=255)
                                                                                                                                      aaTCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTa             <  1:105280/149‑1 (MQ=255)
                                                                                                                                       aTCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTAc            <  2:132570/149‑1 (MQ=255)
                                                                                                                                       aTCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTAc            <  1:215550/149‑1 (MQ=255)
                                                                                                                                           gATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACACCGAACCTCGTTTCGTTGTGCATTCGTACcaca        >  1:135258/1‑149 (MQ=255)
                                                                                                                                                  atcgatGAGGTGCAACAGGACGCGGCAACGTACCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGa  >  2:25744/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCTTCGGCTTCTACCTTATCCAGCAGATCGATCTTGTTGAACACTAACCAACGCGGTTTCGTCGCCAGATCCTGGCTGTATTTTTCCAGCTCGCTGATAATAATACGCGCGTTTTCAACCGGATCGGTGCCGTCAATCGGATCGATATCGATGAGGTGCAACAGGACGCGGCAACGTTCCAGGTGCTTCAGGAAGCGAATGCCCAGACCTGCGCCTTCCGCAGCGCCTTCAATCAGTCCTGGAATATCGGCAACAACGAAGCTCTTTTCGTTGTCCATTCGTACCACACCCAGA  >  NC_000913/3330871‑3331164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: