New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4377082 =109 (1.320)3 (0.040) 3/256 NT 2.8% coding (208/318 nt) sugE multidrug efflux system protein
?NC_000913 4377120 = 99 (1.240)coding (246/318 nt) sugE multidrug efflux system protein
Rejected: Frequency below/above cutoff threshold.

TTTGATGTCAGTAAATTTATTTGGGTACAGCAGCCTGGTAGTTAGTGAGTGCTGAGTTTCAGACCAATAATCCCCAATACGATTAACGCCAGACTCGCCAGGCGCATCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4377233‑4377082
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgcTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGT  >  NC_000913/4377120‑4377267
                                                                                                                                                                                                                                                                                                            
gTTGATGTCAGTAAATTTATTTGGGTACAGCAGCCTGGTAGTTAGTGAGTGCTGAGTTTCAGACCAATAATCGCCAATACGATTAACGCCAGACTCGCCAGGCGCTTCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGC                                                                                                                                                         >  3:137770/2‑149
TTTGATGTCAGTAAATTTATTTGGGTACAGCAGCCTGGTAGTTAGTGAGTGCTGAGTTTCAGACCAATAATCCCCAATACGATTAACGCCAGACTCGCCAGGCGCATCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGC                                                                                                                                                         >  2:318885/1‑149
TTTGATGTCAGTAAATTTATTTGGGTACAGCAGCCTGGTAGTTAGTGAGTGCTGAGTTTCAGACCAATAATCCCCAATACGATTAACGCCAGACTCGCCAGGCGCATCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGC                                                                                                                                                         >  7:86343/1‑149
  TGATGTCAGTAAATTTATTTGGGTACAGCAGCCTGGTAGTTAGTGAGTGCTGAGTTTCAGACCAATAATCCCCAATACGATTAACGCCAGACTCGCCAGGCGCATCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGCCG                                                                                                                                                       >  4:9948/1‑149
                                                                                                  CAGGCGCATCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGCCGC‑‑GCCGACTGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAAT                                                      >  6:97063/1‑148
                                                                                                               TTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAA                                                   <  1:67661/140‑1
                                                                                                               TTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGCCGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAA                                                   >  2:67661/1‑140
                                                                                                                                                     CGCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCT    >  1:264864/1‑149
                                                                                                                                                      GCTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTCAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTG   <  2:363635/149‑1
                                                                                                                                                       CTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGT  <  6:340883/149‑1
                                                                                                                                                                                                                                                                                                            
TTTGATGTCAGTAAATTTATTTGGGTACAGCAGCCTGGTAGTTAGTGAGTGCTGAGTTTCAGACCAATAATCCCCAATACGATTAACGCCAGACTCGCCAGGCGCATCGGGTTAGCGGACTCACCGAGCAGCACAATGCCGGTTATGGCCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4377233‑4377082
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccgcTAACCCGATGCGCCTGGCGAGTCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGT  >  NC_000913/4377120‑4377267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.