Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 725,523 T→A 6.0% Q505L (CAG→CTG)  kdpB ← potassium translocating ATPase, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913725,5230TA 6.0% 250.8 / 3.9 79Q505L (CAG→CTG) kdpBpotassium translocating ATPase, subunit B
Reads supporting (aligned to +/- strand):  ref base T (40/33);  new base A (3/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGCTGGGCGAAGCG  >  NC_000913/725382‑725671
                                                                                                                                             |                                                                                                                                                    
tCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGAt                                                                                                                                               <  6:126267/149‑1 (MQ=255)
  gagaTCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTCCCAAACGACCTTCCGCCTGATACTGACGGATc                                                                                                                                              >  5:90271/1‑148 (MQ=255)
  gagaTCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATc                                                                                                                                              >  6:345979/1‑148 (MQ=255)
  gagaTCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATc                                                                                                                                              >  2:164301/1‑148 (MQ=255)
   agaTCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCaa                                                                                                                                            >  8:230381/1‑149 (MQ=255)
             tatTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTg                                                                                                                                  <  3:250717/149‑1 (MQ=255)
                  cccGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTc                                                                                                                             >  7:158614/1‑149 (MQ=255)
                    cGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCg                                                                                                                           <  4:85166/149‑1 (MQ=255)
                      ccTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACAGACGGATCAATGCCAGCTTGGCCTCCGgt                                                                                                                         >  7:290854/1‑149 (MQ=255)
                      ccTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACAGACGGATCAATGCCAGCTTGGCCTCCGgt                                                                                                                         >  7:285726/1‑149 (MQ=255)
                        tctTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACGGACGGATCAATGCCAGCTTGGCCTCCGgtgt                                                                                                                       >  6:136528/1‑149 (MQ=255)
                         ctTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTc                                                                                                                      >  1:255590/1‑149 (MQ=255)
                                      ggTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCgaga                                                                                                          <  5:345979/148‑1 (MQ=255)
                                      ggTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCgag                                                                                                           <  7:230381/147‑1 (MQ=255)
                                            ggAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGc                                                                                                                                <  6:166161/120‑1 (MQ=255)
                                            ggAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGATACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAatcat                                                                                                   <  4:102577/149‑1 (MQ=255)
                                             gAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAatcatc                                                                                                  <  3:289691/149‑1 (MQ=255)
                                               gTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCAGCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGa                                                                                                <  3:45550/149‑1 (MQ=255)
                                                   aTCGCCCCCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACAcc                                                                                            >  2:43335/1‑149 (MQ=255)
                                                    tCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGGAAATCATCGACAccc                                                                                           >  1:185948/1‑149 (MQ=255)
                                                        cACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCtt                                                                                       <  4:154522/149‑1 (MQ=255)
                                                         aCCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGAAGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTc                                                                                      >  2:48832/1‑149 (MQ=255)
                                                          ccGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTcc                                                                                     >  7:194378/1‑149 (MQ=255)
                                                          ccGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACAGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAATTCATCGACACCCGCTTcc                                                                                     >  7:194379/1‑149 (MQ=255)
                                                           cgcgACATCTGCCTGCGCCAGCGCCGGAGCAGCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCg                                                                                    <  5:165272/149‑1 (MQ=255)
                                                             cgACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCgca                                                                                  <  3:32088/149‑1 (MQ=255)
                                                             cgACATCTGCCTGCGCCAGCGCCGGAGCATCGTGGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATAATGACGGATCAATGCCAGCTTGGCCTCCGGGGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCgca                                                                                  <  6:90271/149‑1 (MQ=255)
                                                                cATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGaaa                                                                                                        >  2:290125/1‑124 (MQ=255)
                                                                cATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGaaa                                                                                                        <  1:290125/124‑1 (MQ=255)
                                                                 aTCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGCCCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGCCCCCCGCTTCcgccgc                                                                                >  7:150600/1‑147 (MQ=255)
                                                                   ctgcctgcGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGATTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCCTCAATc                                                                            >  6:169743/1‑149 (MQ=255)
                                                                      cctgcGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCTGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGCTCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATcgcg                                                                         <  6:115186/149‑1 (MQ=255)
                                                                      cctgcGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAAt                                                                             <  2:255590/145‑1 (MQ=255)
                                                                        tgcGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCgcggc                                                                       >  1:113148/1‑149 (MQ=255)
                                                                         gcgcCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCCGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGCCACCCGCTTCCGCAGCAATCgcggcg                                                                      >  3:103656/1‑149 (MQ=255)
                                                                          cgcCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCgcggc                                                                       <  8:133188/147‑1 (MQ=255)
                                                                          cgcCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGcggcgg                                                                     <  3:257123/149‑1 (MQ=255)
                                                                               gcgcCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTc                                                                <  5:268072/149‑1 (MQ=255)
                                                                               gcgcCGGAACATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAatcat                                                                                                   >  7:317885/1‑114 (MQ=255)
                                                                               gcgcCGGAACATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAatcat                                                                                                   <  8:317885/114‑1 (MQ=255)
                                                                                 gcCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGt                                                                 >  7:156394/1‑146 (MQ=255)
                                                                                        cATCGTTGGTGCAGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCAACGACACCCGCTTCCGCAGCAAACGCGGCGGCAGTCATACGGTTa                                                       >  4:60111/1‑149 (MQ=255)
                                                                                         aTCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTAt                                                      <  3:60111/149‑1 (MQ=255)
                                                                                          tCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATc                                                     >  4:252459/1‑149 (MQ=255)
                                                                                               ggTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACAGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCg                                                 <  8:290854/148‑1 (MQ=255)
                                                                                               ggTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACAGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCg                                                 <  8:285726/148‑1 (MQ=255)
                                                                                                gTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCgg                                                <  4:243882/148‑1 (MQ=255)
                                                                                                 tGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGt                                               >  2:312848/1‑148 (MQ=255)
                                                                                                   ccGTCGCCGGTCATCGCAACCAAACGACCTCCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCGCCGGGGTCGCTGCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAat                                            <  8:1199/149‑1 (MQ=255)
                                                                                                    cGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAatc                                           <  8:208323/149‑1 (MQ=255)
                                                                                                     gTCGCCGGTCATCGCTACCAAACGCCCTTCCGCCTGATACTGCCGGATCAATGCCCGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAatca                                          >  4:193950/1‑149 (MQ=255)
                                                                                                        gCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAAtcatca                                       <  1:164301/149‑1 (MQ=255)
                                                                                                         ccGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCAc                                      >  4:302280/1‑149 (MQ=255)
                                                                                                          cGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTAt                                                      >  2:218088/1‑132 (MQ=255)
                                                                                                          cGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTAt                                                      <  1:218088/132‑1 (MQ=255)
                                                                                                            gTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGGAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGt                                   <  2:185948/149‑1 (MQ=255)
                                                                                                            gTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGt                                   >  5:211434/1‑149 (MQ=255)
                                                                                                                tCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGtttt                                >  8:198194/1‑148 (MQ=255)
                                                                                                                   cTACCAAACGACCTTCCTCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATg                            >  3:128477/1‑149 (MQ=255)
                                                                                                                   cTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATg                            >  7:307609/1‑149 (MQ=255)
                                                                                                                    tACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATga                           >  2:3288/1‑148 (MQ=255)
                                                                                                                      ccAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGcc                          >  7:177643/1‑148 (MQ=255)
                                                                                                                      ccAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGcc                          >  8:359879/1‑148 (MQ=255)
                                                                                                                       cAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCa                        <  8:158614/149‑1 (MQ=255)
                                                                                                                         aaCGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCAtt                      >  1:132058/1‑149 (MQ=255)
                                                                                                                         aaCGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCAtt                      >  1:37290/1‑149 (MQ=255)
                                                                                                                          aCGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCAttt                     <  1:43335/149‑1 (MQ=255)
                                                                                                                             aCCTTCCGCCTGATACTGACGGCTCAATGCCAGCTTGGCCTCCGGTGTCCCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGGCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCCTTTTgc                  >  7:280827/1‑149 (MQ=255)
                                                                                                                               cTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTgcgc                >  5:61588/1‑149 (MQ=255)
                                                                                                                               cTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGAGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTgcg                 >  2:9115/1‑148 (MQ=255)
                                                                                                                                ttCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTgcg                 >  5:236371/1‑147 (MQ=255)
                                                                                                                                ttCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTGTTAATGCCCATTTTgcgcc               >  5:282015/1‑148 (MQ=255)
                                                                                                                                ttCCGCCTGATACTGACGGATCAATGCCAGCTTGGAATCCGGTGTCGATTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCCGCAGACAGACGGTTATCGCCGGTATTCATGACCGTTTTAATGCCCATTTTgcg                 >  3:249852/1‑147 (MQ=255)
                                                                                                                                 tCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAGAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAg              >  7:31766/1‑149 (MQ=255)
                                                                                                                                   cGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGCt            >  8:267272/1‑149 (MQ=255)
                                                                                                                                   cGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGATTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGc             <  1:69325/148‑1 (MQ=255)
                                                                                                                                          tACTGACGGATCTATGCCAGCTTGGCCTCCTGTGTCGCTTCTTCGAGAAAATCATCGAAACCCGCTTCCGCAGCAATCGCGGCTGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCACATTTTGCGCAGCTGGGCGaa     <  2:113148/149‑1 (MQ=255)
                                                                                                                                            cTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGCTGGGCGAAgc   <  6:211434/149‑1 (MQ=255)
                                                                                                                                            cTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGCTGGGCGAAgc   <  8:307609/149‑1 (MQ=255)
                                                                                                                                             tGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGCTGGGCGAAgcg  >  5:260577/1‑149 (MQ=255)
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TCGAGATCGACCATATTGCCCGCCTCTTTCGCCGCCTGGGTGCCGGAGTTCATCGCCACCGCGACATCTGCCTGCGCCAGCGCCGGAGCATCGTTGGTGCCGTCGCCGGTCATCGCTACCAAACGACCTTCCGCCTGATACTGACGGATCAATGCCAGCTTGGCCTCCGGTGTCGCTTCGGCGAGAAAATCATCGACACCCGCTTCCGCAGCAATCGCGGCGGCAGTCAGACGGTTATCGCCGGTAATCATCACCGTTTTAATGCCCATTTTGCGCAGCTGGGCGAAGCG  >  NC_000913/725382‑725671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: