Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,483,043 C→A 5.7% intergenic (‑183/‑18) azoR ← / → hrpA NADH‑azoreductase, FMN‑dependent/putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,483,0430CA 5.7% 338.3 / 5.5 98intergenic (‑183/‑18)azoR/hrpANADH‑azoreductase, FMN‑dependent/putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (43/49);  new base A (3/3);  total (46/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGCTTGTTGACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCTGATGCAC  >  NC_000913/1482897‑1483187
                                                                                                                                                  |                                                                                                                                                
cGTTGCTTGTTGACACTTTATTCACAATCCTTCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAATAAAATTAATCAGGCAGacta                                                                                                                                                <  1:58641/149‑1 (MQ=255)
cGTTGCTTGTTGACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGacta                                                                                                                                                >  5:126887/1‑149 (MQ=255)
 gTTGCTTGTTGACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTAc                                                                                                                                               >  6:289054‑M2/1‑148 (MQ=255)
 gTTGCTTGTTGACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTGCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTAc                                                                                                                                               >  4:150915‑M2/1‑148 (MQ=255)
      ttgttgACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTActgcc                                                                                                                                           <  8:223339‑M2/148‑6 (MQ=255)
         ttgACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCAc                                                                                                                                        <  7:223339/148‑1 (MQ=255)
             cacTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACg                                                                                                                                   >  1:318604/1‑149 (MQ=255)
              acTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGt                                                                                                                                  >  6:87823/1‑149 (MQ=255)
              acTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGt                                                                                                                                  >  8:149245/1‑149 (MQ=255)
               cTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGTAGTctgcctgattaattt                                                                                                                                 <  1:154810‑M2/149‑16 (MQ=255)
                tttATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGATTTCATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTTACCACTAACGTTa                                                                                                                                >  1:30268/1‑149 (MQ=255)
                tttATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTa                                                                                                                                >  5:81762/1‑149 (MQ=255)
                  tATTCACAATCCTGCCAGAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCCTAGAAAGCCGCGATGTGTTACTCTATATCTATCATTTAAAAGAATATTAATCAGGCAGACTACTGGCCACTTACGTTAt                                                                                                                               <  6:91005/148‑1 (MQ=255)
                  tATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTa                                                                                                                                <  5:181653/147‑1 (MQ=255)
                  tATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTa                                                                                                                                <  3:105234/147‑1 (MQ=255)
                  tATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTAt                                                                                                                               <  8:226214/148‑1 (MQ=255)
                   aTTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGAATACTGCCCACTAACGTTATg                                                                                                                              <  5:195921/148‑1 (MQ=255)
                    ttCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGTAGTctgcctgattaattttcttt                                                                                                                            >  2:154810‑M2/1‑129 (MQ=255)
                     tCACAATCCTGCCACAGAGATAGCGCAATAAATAGAAGCCGAAGTTCGAATTTATTGAACAACGCATAGAAAGCCTCGATGTGGTACTCTATATATATCATATAAAAGAAAATTAATAATGCAGAATACTGCCCAATAAAGTTAtcaa                                                                                                                            >  1:253404/1‑145 (MQ=255)
                      cacaATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACt                                                                                                                                       <  3:283265/136‑1 (MQ=255)
                      cacaATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACt                                                                                                                                       >  4:283265/1‑136 (MQ=255)
                       acaATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAg                                                                                                                          >  8:67421/1‑148 (MQ=255)
                       acaATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAg                                                                                                                          <  5:162022/148‑1 (MQ=255)
                        caATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGaa                                                                                                                        <  8:282299/149‑1 (MQ=255)
                        caATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGaa                                                                                                                        >  2:145170/1‑149 (MQ=255)
                         aaTCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATTTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGaac                                                                                                                       <  2:304205/149‑1 (MQ=255)
                         aaTCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGaa                                                                                                                        >  1:35225/1‑148 (MQ=255)
                           tCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGaacaa                                                                                                                     <  7:268634/149‑1 (MQ=255)
                             cTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGaacaac                                                                                                                    <  1:145170/148‑1 (MQ=255)
                              tGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAacaaca                                                                                                                   >  8:52264/1‑148 (MQ=255)
                               gCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAAcaacaa                                                                                                                  >  8:149975/1‑148 (MQ=255)
                                ccACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACaaa                                                                                                                 >  8:187713/1‑148 (MQ=255)
                                 cacaGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACaaaaa                                                                                                               >  2:251340/1‑149 (MQ=255)
                                     gagaTAGCTCAATAAATCGAAGCATATGTTCGAATTTATTGAACAAAGCATAGAAAGCCGCGATGTGGTACTCTATATCTATAATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTACCGTTATGACAGAACAACAAAAATTGa                                                                                                           <  1:314853/149‑1 (MQ=255)
                                       gaTAGCGAAATAAATCGAAGCCTATGTTCGAATTCAATGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGAc                                                                                                          <  2:30268/148‑1 (MQ=255)
                                           gcgcAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTa                                                                                                     >  6:240090/1‑149 (MQ=255)
                                           gcgcAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTa                                                                                                     <  6:108484/149‑1 (MQ=255)
                                           gcgcAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTa                                                                                                     <  5:227433/149‑1 (MQ=255)
                                            cgcAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTAc                                                                                                    >  4:75133/1‑149 (MQ=255)
                                              cAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACgg                                                                                                  <  2:35481/149‑1 (MQ=255)
                                                aTAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGcc                                                                                                >  4:96502/1‑149 (MQ=255)
                                                aTAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGcc                                                                                                <  7:132540/149‑1 (MQ=255)
                                                aTAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGcc                                                                                                <  3:96502/149‑1 (MQ=255)
                                                aTAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGACCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGcc                                                                                                >  7:361899/1‑149 (MQ=255)
                                                   aaTCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCtt                                                                                              <  4:220758/148‑1 (MQ=255)
                                                       gAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTgcagc                                                                                         <  1:251340/149‑1 (MQ=255)
                                                                 ttCGAATTTATTTAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTgg                                                                                <  6:150008/148‑1 (MQ=255)
                                                                 ttCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGa                                                                               <  7:52264/149‑1 (MQ=255)
                                                                 ttCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTACCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGa                                                                               >  6:295317/1‑149 (MQ=255)
                                                                  tCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTa                                                                                                     >  5:70763/1‑126 (MQ=255)
                                                                  tCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTa                                                                                                     <  6:70763/126‑1 (MQ=255)
                                                                   cGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGAtt                                                                             >  5:121371/1‑149 (MQ=255)
                                                                    gAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGAtt                                                                             <  5:87823/148‑1 (MQ=255)
                                                                    gAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGATATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGAGGCTGGATTc                                                                            >  5:308254/1‑149 (MQ=255)
                                                                     aaTTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGTAGTctgcctgattaattttcttttaa                                                                                                                         <  4:205880‑M2/103‑24 (MQ=255)
                                                                     aaTTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGTAGTctgcctgattaattttcttttaa                                                                                                                         >  3:205880‑M2/1‑80 (MQ=255)
                                                                      aattATTGAACAACGCATATAAAGCCGCTATGTCGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACATTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGc                                                                          <  3:71450/147‑1 (MQ=255)
                                                                      aTTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGc                                                                          >  3:289437/1‑149 (MQ=255)
                                                                         tATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTg                                                                        <  2:318604/148‑1 (MQ=255)
                                                                         tATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTg                                                                        <  6:81762/148‑1 (MQ=255)
                                                                          aTTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGa                                                                       <  6:162733/148‑1 (MQ=255)
                                                                          aTTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGa                                                                       <  4:289437/148‑1 (MQ=255)
                                                                           ttGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGAtg                                                                     >  2:80873/1‑149 (MQ=255)
                                                                            tGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGa                                                                       <  2:143677/146‑1 (MQ=255)
                                                                            tGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGa                                                                       >  1:143677/1‑146 (MQ=255)
                                                                            tGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGa                                                                       >  1:138586/1‑146 (MQ=255)
                                                                            tGAACAACGAATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGa                                                                       <  2:138586/146‑1 (MQ=255)
                                                                              aacaacGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGCCTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGAtgctg                                                                  >  7:140101/1‑149 (MQ=255)
                                                                              aacaacCCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGAtgctg                                                                  >  8:323231/1‑149 (MQ=255)
                                                                               acaacGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGAtgctgc                                                                 <  7:149975/149‑1 (MQ=255)
                                                                                 aacGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGAt                                                                      >  4:69320/1‑142 (MQ=255)
                                                                                 aacGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGAt                                                                      <  3:69320/142‑1 (MQ=255)
                                                                                 aacGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGt                                                               <  4:309892/149‑1 (MQ=255)
                                                                                      aTAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGAc                                                                                                                            >  2:48510/1‑83 (MQ=255)
                                                                                          aaaGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTg                                                      >  4:50801/1‑149 (MQ=255)
                                                                                             gCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGt                                                   <  7:323231/149‑1 (MQ=255)
                                                                                                 cgATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGttttt                                               <  7:67421/149‑1 (MQ=255)
                                                                                                  gATGTGGTAATCTATATCTATAATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTtc                                              <  2:253404/149‑1 (MQ=255)
                                                                                                      tgGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGc                                          >  4:182969/1‑149 (MQ=255)
                                                                                                        gTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCg                                        >  5:145808/1‑149 (MQ=255)
                                                                                                          aatctATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTc                                      >  1:128843/3‑149 (MQ=255)
                                                                                                            tctATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCg                                                                                     <  6:251430/100‑1 (MQ=255)
                                                                                                            tctATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCg                                                                                     >  5:251430/1‑100 (MQ=255)
                                                                                                                tatctatcATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACg                                >  5:337987/1‑149 (MQ=255)
                                                                                                                    tatcATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGt                            >  2:260549/1‑149 (MQ=255)
                                                                                                                    tatcATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGt                            >  7:99411/1‑149 (MQ=255)
                                                                                                                      tcATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTga                          <  4:339680/149‑1 (MQ=255)
                                                                                                                          ttAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCTGCTGGATTCGCTGATGCTGCGTGACAGACTGCGATTTTCTCGCCGTCTGCACGGCGTgaag                        >  3:251478/1‑147 (MQ=255)
                                                                                                                           tAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTgaagaa                      <  8:26862/148‑1 (MQ=255)
                                                                                                                           tAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTATGCAATGCGTgaagaa                      <  2:223650/148‑1 (MQ=255)
                                                                                                                            aaaaGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGaagaag                     <  5:88248/148‑1 (MQ=255)
                                                                                                                               aGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTa                 <  3:182969/149‑1 (MQ=255)
                                                                                                                                gAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGt                                                               >  8:288839/1‑102 (MQ=255)
                                                                                                                                gAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGt                                                               <  7:288839/102‑1 (MQ=255)
                                                                                                                                  aaaTTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTaaaa              <  7:149245/149‑1 (MQ=255)
                                                                                                                                  aaaTTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTaaaa              <  4:254129/149‑1 (MQ=255)
                                                                                                                                        aTCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCt         <  3:75133/148‑1 (MQ=255)
                                                                                                                                         tCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCTGa       <  8:288207/149‑1 (MQ=255)
                                                                                                                                           aGGCAGACTACTGCTCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCTGATg     >  1:52487/1‑149 (MQ=255)
                                                                                                                                               aGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCTGATGcac  >  2:349310/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGTTGCTTGTTGACACTTTATTCACAATCCTGCCACAGAGATAGCGCAATAAATCGAAGCCTATGTTCGAATTTATTGAACAACGCATAGAAAGCCGCGATGTGGTACTCTATATCTATCATTTAAAAGAAAATTAATCAGGCAGACTACTGCCCACTAACGTTATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATGCTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCTGATGCAC  >  NC_000913/1482897‑1483187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: