Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,342,538	G→C	5.7%	G544G (GGC→GGG)	yfaL ←	adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,342,538	0	G	C	5.7%	237.7 / 3.4	70	G544G (GGC→GGG)	yfaL	adhesin
Reads supporting (aligned to +/- strand):  ref base G (27/39);  new base C (2/2);  total (29/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATC  >  NC_000913/2342391‑2342675                                                                                                                                                    |                                                                                                                                          ataGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTcg                                                                                                                                           <  6:234859/148‑1 (MQ=255)  taGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCAAGTAGACGCCATCTTTCCCGTcgc                                                                                                                                          <  6:125753/148‑1 (MQ=255)     tGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCgccgcc                                                                                                                                      >  4:154168/1‑149 (MQ=255)       ggTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGa                                                                                                                                    <  3:21761/149‑1 (MQ=255)       ggTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGa                                                                                                                                    <  3:315384/149‑1 (MQ=255)        gTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCACAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGa                                                                                                                                    >  8:221449/1‑148 (MQ=255)         tATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCTCCACGTAGACGCCATCTTTCCCGTCGCCGCCGata                                                                                                                                  <  5:131783/149‑1 (MQ=255)         tATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGata                                                                                                                                  >  2:18815/1‑149 (MQ=255)              ccactcTGCCAGTTGGCGCTCACCATCAGCGTACCGGAGGCGATGTTTGGTGTGCCCAAATAACTATTATTGTTTGCCAGACATACCTGCCCGTCGCTCGCATCGACCCCCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCaa                                                                                                                             <  4:256472/144‑1 (MQ=255)               cAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGc                                                                                                                               <  2:144036/146‑1 (MQ=255)                 aGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCTATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAAtt                                                                                                                          <  5:177539/149‑1 (MQ=255)                 aGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAAtt                                                                                                                          <  8:274126/149‑1 (MQ=255)                      cGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCGTCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGt                                                                                                                        <  6:257524/146‑1 (MQ=255)                             tCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTg                                                                                                               >  1:337516/1‑148 (MQ=255)                               gCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCaa                                                                                                            >  3:68580/1‑149 (MQ=255)                                   aCCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGa                                                                                                          <  3:154168/147‑1 (MQ=255)                                   aCCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGAt                                                                                                         <  6:161653/148‑1 (MQ=255)                                     cATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCaaa                                                                                                                            <  1:243558/127‑1 (MQ=255)                                     cATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCaaa                                                                                                                            >  2:243558/1‑127 (MQ=255)                                      ctccgCGTACCGTAGGCTATTTGTTTTCGGCCCAAATAACTATTATTGATTGCCAGACTAACCTTCCCGTCGCTCGCCTCGACCACCACGTAGACGCCATCTTTACCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTAc                                                                                                     <  2:40001/145‑1 (MQ=255)                                      aTCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTAACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTAc                                                                                                     <  1:150583/149‑1 (MQ=255)                                      aTCAGCGGACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTAc                                                                                                     <  5:279794/149‑1 (MQ=255)                                       tCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCCCCGCCGATATGCAAAtt                                                                                                                          >  1:86749/1‑127 (MQ=255)                                       tCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCCCCGCCGATATGCAAAtt                                                                                                                          >  1:81769/1‑127 (MQ=255)                                       tCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCCCCGCCGATATGCAAAtt                                                                                                                          <  2:81769/127‑1 (MQ=255)                                       tCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCCCCGCCGATATGCAAAtt                                                                                                                          <  2:86749/127‑1 (MQ=255)                                         aGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACca                                                                                                   >  6:70589/1‑148 (MQ=255)                                             tACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATcc                                                                                              >  8:142121/1‑149 (MQ=255)                                                cGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGa                                                                                           >  1:142039/1‑149 (MQ=255)                                                cGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGa                                                                                           >  1:368535/1‑149 (MQ=255)                                                  gAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGACGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACt                                                                                         <  2:197343/149‑1 (MQ=255)                                                     gCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGc                                                                                       <  6:294572/148‑1 (MQ=255)                                                       gATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGa                                                                                           <  3:165025/142‑1 (MQ=255)                                                       gATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGa                                                                                           >  4:165025/1‑142 (MQ=255)                                                            gtgttgtgCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAAcc                                                                               >  4:103771/1‑149 (MQ=255)                                                                    cccAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCgct                                                                       >  3:19125/1‑149 (MQ=255)                                                                      cAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCCCCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCgctgc                                                                     >  7:232933/1‑149 (MQ=255)                                                                               attattGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACcaca                                                                                                 <  1:140963/112‑1 (MQ=255)                                                                               attattGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACcaca                                                                                                 >  2:140963/1‑112 (MQ=255)                                                                                ttattGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTCCCCCATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGa                                                            >  6:331798/1‑148 (MQ=255)                                                                                ttattGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGACACCTGCAAGATCTACAACATCCTGACTGCCGATAACCGTTCCGCTGCTGCAACTGAc                                                           >  1:42011/1‑149 (MQ=255)                                                                                   ttGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAAc                                                                                >  7:217807/1‑125 (MQ=255)                                                                                   ttGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAAc                                                                                <  8:217807/125‑1 (MQ=255)                                                                                   ttGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCAGATAACCGTTCCGCTGCTGCCACTGACTTc                                                        <  2:72343/149‑1 (MQ=255)                                                                                      tttGCCAGACTTACCTGCCCGTCGCTCGCATCGACCCCCACGTAGCCGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAg                                                     >  3:321505/1‑149 (MQ=255)                                                                                      tttGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCaa                                                      <  1:18815/148‑1 (MQ=255)                                                                                      tttGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCaa                                                      <  2:363353/148‑1 (MQ=255)                                                                                      tttGCCAGACTTACCTGCCCGTCGCTCGCAGCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCaa                                                      <  2:337516/148‑1 (MQ=255)                                                                                        tGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAgg                                                    <  7:375065/148‑1 (MQ=255)                                                                                         gCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGc                                                   >  7:161724/1‑148 (MQ=255)                                                                                          ccAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCa                                                 <  4:68580/149‑1 (MQ=255)                                                                                             gACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCg                                              >  7:68989/1‑149 (MQ=255)                                                                                              aCTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGt                                             >  1:203710/1‑149 (MQ=255)                                                                                                  aCCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGt                                          <  3:103771/148‑1 (MQ=255)                                                                                                       cccGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCTGTGATATTTAATTTTTCGGCTACTTCATTTTATAACACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTatga                                      <  4:346306/147‑1 (MQ=255)                                                                                                       cccGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTatgatg                                    >  1:175980/1‑149 (MQ=255)                                                                                                         cGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGAtgtg                                  <  2:368535/149‑1 (MQ=255)                                                                                                          ggcgctcgcCTCGACCACCACGTAGACGCCATCTTTCCCGGCGCCGCCGATATGCCAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGc                                 <  4:277961/147‑1 (MQ=255)                                                                                                                 gcATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGctc                            <  1:177648/147‑1 (MQ=255)                                                                                                                  cATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGc                         <  5:70589/149‑1 (MQ=255)                                                                                                                        ccaccaCGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTg                   <  2:142039/149‑1 (MQ=255)                                                                                                                         caccacGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTg                   >  6:281892/1‑148 (MQ=255)                                                                                                                         caccacGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTg                   >  8:286186/1‑148 (MQ=255)                                                                                                                           ccacGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCAACCACATCCTGATTGCCGATACCCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGgag                <  5:117530/149‑1 (MQ=255)                                                                                                                                 aGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTAc                                                                                                     <  4:317179/58‑1 (MQ=255)                                                                                                                                 aGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTAc                                                                                                     >  3:317179/1‑58 (MQ=255)                                                                                                                                  gACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCa          <  8:68989/148‑1 (MQ=255)                                                                                                                                  gACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTGCCGCTGCAGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCa          <  5:331798/148‑1 (MQ=255)                                                                                                                                    cGCTATCTTTCCCGTCGCCGGCGTTATGCAAATTGTCGCCACCTTCAAGATCTTCCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCAATGAGTTCAAGGCCATCGTTGTATGATGTGCCGGTCTGCCAGGTGGAGAAATCaga        <  2:42011/148‑1 (MQ=255)                                                                                                                                     gCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCagaga      <  5:281892/149‑1 (MQ=255)                                                                                                                                     gCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCaga        >  6:150090/1‑147 (MQ=255)                                                                                                                                        aTCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCaga        >  8:210590/1‑144 (MQ=255)                                                                                                                                             tCCCGTCGCCGCCGATATGCATATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTTCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATc  <  7:221449/145‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                          ATAGTGGGTATCTCCAAGCTGCGAGTTGTCGCTCACCATCAGCGTACCGGAGGCGATTTGTGTTGTGCCCAAATAACTATTATTGTTTGCCAGACTTACCTGCCCGTCGCTCGCATCGACCACCACGTAGACGCCATCTTTCCCGTCGCCGCCGATATGCAAATTGTCGCCACCTGCAAGATCTACCACATCCTGACTGCCGATAACCGTTCCGCTGCTGCCACTGACTTCAAGGCCATCGTTGTATGATGTGCCGCTCTGCCAGGTGGAGAAATCAGAGAGATC  >  NC_000913/2342391‑2342675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑