| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 399,993 | C→A | 11.0% | V311L (GTG→TTG) | ddlA ← | D‑alanine‑D‑alanine ligase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 399,993 | 0 | C | A | 11.0% | 183.5 / 2.4 | 61 | V311L (GTG→TTG) | ddlA | D‑alanine‑D‑alanine ligase A |
| Reads supporting (aligned to +/- strand): ref base C (21/32); new base A (6/2); total (27/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCG > NC_000913/399846‑400138 | aTGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACt > 7:13450/1‑149 (MQ=255) cggtataCGCAGCGTGACGCTCCAGCGGCAGTTCAATCAAACGTGTGATGAGATCGGTGTAACCCATACCGCTGGCTTGCAACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATAACCACTTCg < 6:323793/146‑1 (MQ=255) cGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTc < 5:180375/148‑1 (MQ=255) ttATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGtt < 2:150044/149‑1 (MQ=255) cGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTCGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTctct < 5:139240/148‑1 (MQ=255) gCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTgg < 5:352998/149‑1 (MQ=255) gCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTgg < 5:74626/149‑1 (MQ=255) tCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAacac < 4:344662/149‑1 (MQ=255) cAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAacac < 8:157678/147‑1 (MQ=255) cAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCGCTGGGGTTAAAAACCCGTCTacac > 7:167061/1‑149 (MQ=255) aGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTATATCTGGTTAAAAACACGTCTAAAcg > 2:64013/1‑149 (MQ=255) gTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACAcgc > 3:62913/1‑149 (MQ=255) gTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACAcgc > 5:50400/1‑149 (MQ=255) gTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACATCCTTATCTGATGTTAAAAACACGTCTACAcgc > 2:372158/1‑149 (MQ=255) tcaaACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATg > 7:94558/1‑149 (MQ=255) aaaCGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGcc > 7:338881/1‑149 (MQ=255) cGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgc < 8:161086/149‑1 (MQ=255) gtgtGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTgcg > 7:272912/1‑149 (MQ=255) aTCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACg > 3:241399/1‑149 (MQ=255) tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGa < 8:13450/149‑1 (MQ=255) tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGa < 7:98404/149‑1 (MQ=255) tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGa < 1:372158/149‑1 (MQ=255) tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGa < 1:142902/149‑1 (MQ=255) tgtAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTAGATCTCGTTGATCACCAAATCATTAAAAAAAAAAAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGa > 4:68159/1‑149 (MQ=255) aCCTAGACCGCTGGCTTGCCACAGCTTAGGATACTTACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGGTAAAAACACGTATACACGCGCCATGCCTGCGCATCCCAACGTTTGATaa < 5:317628/148‑1 (MQ=255) ccAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGcc > 2:330120/1‑149 (MQ=255) gACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGa < 6:50400/149‑1 (MQ=255) gCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGc < 4:124577/148‑1 (MQ=255) cacaGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTAGATCTCGTTGATCACCAAATCATTAAAAAAAAATAAAAACACGTCAACACGCGCAAGGCCTGCGCATCCCAACGATTGATAAGCCTGAACGGCAATCGccca > 2:291188/1‑148 (MQ=255) gCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATc < 4:241399/149‑1 (MQ=255) gCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATc < 8:21223/149‑1 (MQ=255) ttCGGATACATACTTATATTAGTGAAGCCAGGCAGGGTGTTGAGCTCGTTGATAACCAATTCGTTCTCTGGGGTTAAAAAAACGTCTACACGCGCCATGCCTGCGCATACCAACGTTTGATAAGCCTGAACGGCAATCGCACGGATCtt < 3:328701/149‑1 (MQ=255) cGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCtt < 1:330120/147‑1 (MQ=255) ggATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTAt < 2:162066/148‑1 (MQ=255) atacatacTGATATTAGTGAAGCCAGGCAGGGTGTAGATCTCTTTGATCACCAAATCATTAAAAAAAAAAAAAAACAAGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTCCACGGCAATCGCCCGGATCTTCTCGt > 6:218158/1‑149 (MQ=255) atacatacTGATATTAGTGAAGCCAGGAAGTGTGTTGATCGCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGt < 1:64013/149‑1 (MQ=255) catacTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGATTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGATTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGttta > 7:360718/1‑147 (MQ=255) atacTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGc < 1:216309/91‑1 (MQ=255) atacTGATATTAGTGAAGCCAGGCAGGGTGTAGATCTCGTTGATCACCAAATCATTAAAAAAAAAAAAAAACACGTCTACACGCGCCATGc > 2:216309/1‑91 (MQ=255) tacTGATCTTCGTGAAGCCAGGCAGGGTGTAGATCTCGTTGATCACCAAATCATTAAAAAAAAAAAAAAACACGTCTACACGCGCCATGCAAGCGCATACCAACGTTTGATAAGCCTGAACGGCAATCTCCCGGATCTGATCGTTGAtt > 6:226039/1‑149 (MQ=255) acTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGAttt > 1:202853/1‑149 (MQ=255) tGATATTAGTGAAGCCAGGCAGGGTGTAGATCTCGTTGATCACCAAATCATTAAAAAAAAATAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCt > 8:352054/1‑149 (MQ=255) atatTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTg < 3:313598/148‑1 (MQ=255) atatTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTg < 4:62913/148‑1 (MQ=255) tatTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGgc < 7:122876/149‑1 (MQ=255) tatTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGgc > 7:159808/1‑149 (MQ=255) gTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAAt < 8:272912/149‑1 (MQ=255) tGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATg < 8:94558/149‑1 (MQ=255) gtgtTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTAc < 8:338881/149‑1 (MQ=255) ttGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCa < 3:68159/148‑1 (MQ=255) ttGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCAc > 4:36136/1‑149 (MQ=255) cgaactcGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCAc < 7:270928/144‑1 (MQ=255) aTCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTa < 2:173847/108‑1 (MQ=255) aTCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTa > 1:173847/1‑108 (MQ=255) aTCGCGTTTATCAACACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACttt < 1:102659/149‑1 (MQ=255) gTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCAc > 8:270928/1‑141 (MQ=255) ttGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTcgcg > 7:272060/1‑147 (MQ=255) ttGATCACCAATTCGGTCTCTTGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTcgcg < 8:272060/147‑1 (MQ=255) gATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCAt > 1:332558/1‑149 (MQ=255) tCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCt > 7:10579/1‑149 (MQ=255) tCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCt > 6:311905/1‑149 (MQ=255) ccacTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCg > 1:170443/1‑149 (MQ=255) | ATGCGTTATCCGCAGCGTGACGCTCCAGCGCCAGTTCAATCAAACGTGTGATCAGATCGGTGTAACCCAGACCGCTGGCTTGCCACAGCTTCGGATACATACTGATATTAGTGAAGCCAGGCAGGGTGTTGATCTCGTTGATCACCACTTCGTTCTCTGGGGTTAAAAACACGTCTACACGCGCCATGCCTGCGCATCCCAACGTTTGATAAGCCTGAACGGCAATCGCCCGGATCTTATCGTTGATTTCTGGCGCAATGGCTGCCGGAACTACCACTTTCGCGCCATCTTCG > NC_000913/399846‑400138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |