Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,486,190 A→G 5.8% E606G (GAA→GGA)  evgS → hybrid sensory histidine kinase in two‑component regulatory system with EvgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,486,1900AG 5.8% 318.4 / 7.1 105E606G (GAA→GGA) evgShybrid sensory histidine kinase in two‑component regulatory system with EvgA
Reads supporting (aligned to +/- strand):  ref base A (47/51);  new base G (3/3);  total (50/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 107 total aligned reads displayed.

GTTACGCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATACACACAGGTATTTGAAAT  >  NC_000913/2486044‑2486337
                                                                                                                                                  |                                                                                                                                                   
 ttACGCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACa                                                                                                                                                  >  4:16777/1‑149 (MQ=255)
 ttACGCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCAAAATAGTGCTTTTGAACa                                                                                                                                                  <  3:81733/149‑1 (MQ=255)
   tcggtCAGTTCGTCGTCGTAAAGTGATTCAGGGTGATTTAGAAATACAAATATCATTACGAAAAGCACTCTCGGATTCCTTACCTAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACat                                                                                                                                                 <  3:328384/144‑1 (MQ=255)
    cGCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACatta                                                                                                                                               <  2:326139/149‑1 (MQ=255)
    cGCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACatt                                                                                                                                                <  8:96660/148‑1 (MQ=255)
     gCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACattat                                                                                                                                              >  8:127481/1‑149 (MQ=255)
     gCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACattat                                                                                                                                              >  4:30539/1‑149 (MQ=255)
      cTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACAGAATACAACTTATGTTGTAAACTGGCAATGTAATGTCATTAGTCATAATAGTGCATTTGAACattatt                                                                                                                                             >  3:143824/1‑149 (MQ=255)
       tCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCTTAATAGTGCGTTTGAACATTAttt                                                                                                                                            >  6:191748/1‑149 (MQ=255)
       tCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTAttt                                                                                                                                            >  7:273748/1‑149 (MQ=255)
         aGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTAttt                                                                                                                                            <  7:248644/147‑1 (MQ=255)
         aGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCa                                                                                                                                          <  5:307579/149‑1 (MQ=255)
         aGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCa                                                                                                                                          <  1:298366/149‑1 (MQ=255)
             cgtcgtcgtAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTg                                                                                                                                       <  7:127481/148‑1 (MQ=255)
               tcgtcgtAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCg                                                                                                                                     >  7:97313/1‑148 (MQ=255)
                 gtcgtAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGATAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGa                                                                                                                                   <  6:213994/148‑1 (MQ=255)
                  tcgtAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGAt                                                                                                                                  >  1:235731/1‑148 (MQ=255)
                  tcgtAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACAGTATTTCACTGCGGAt                                                                                                                                  >  5:18537/1‑148 (MQ=255)
                  tcgtAAAGTCATTCAGGGTGATTTAGAAAACCAAATAGCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGAtt                                                                                                                                 <  1:175564/149‑1 (MQ=255)
                    gtAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAAATTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGAGGATtac                                                                                                                               >  1:130948/1‑149 (MQ=255)
                       aaGTCATTCAGGGTTATTTAGAATACCAAATTTTATTCCGAATAGCAATCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAAATGGGAAGGTAATTTCATTAGTCATAATAGTGATTTTGAACATTATTTCACTGCGGAttatta                                                                                                                             <  8:92590/148‑1 (MQ=255)
                            aTTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACaaaaa                                                                                                                       <  3:16777/149‑1 (MQ=255)
                            aTTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACaaaa                                                                                                                        >  4:252480/1‑148 (MQ=255)
                             ttCAGGGTGAATTAGAAAACCAAATATCATTCCGAACAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAAt                                                                                                                      <  2:31275/149‑1 (MQ=255)
                                aGGGGGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCa                                                                                                                   <  6:304013/149‑1 (MQ=255)
                                 gggTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCaa                                                                                                                  >  6:335664/1‑149 (MQ=255)
                                      aTTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTa                                                                                                             >  5:237657/1‑149 (MQ=255)
                                        ttAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTAc                                                                                                            >  3:331998/1‑148 (MQ=255)
                                         tAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCaa                                                                                                                  >  8:348765/1‑141 (MQ=255)
                                         tAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTAcc                                                                                                           <  5:344786/148‑1 (MQ=255)
                                           gAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCAt                                                                                                         >  5:130674/1‑148 (MQ=255)
                                                 cAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATta                                                                                                                                <  7:151530/119‑1 (MQ=255)
                                                 cAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATta                                                                                                                                >  8:151530/1‑119 (MQ=255)
                                                 cAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGaaaa                                                                                                  >  2:189263/1‑149 (MQ=255)
                                                      atCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGt                                                                                              >  6:233893/1‑148 (MQ=255)
                                                        cATTCCGAAAAGCAATCTCGGATTCCTTACAGAATCCAAATTATGTTGTAAACTGGCAAGTTAATGTCATTAGTCATAATAGTGCTTTTGTACATTATTTCACTGCTGATTTTTAGAAAAATGCAATGTTACGATTAGAAAACAGTGa                                                                                            <  3:252480/148‑1 (MQ=255)
                                                         aTTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGAc                                                                                           <  3:317781/148‑1 (MQ=255)
                                                          ttCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACt                                                                                          >  7:257307/1‑148 (MQ=255)
                                                          ttCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACGTATGGTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTGTTGAACATTATTTCACTGCGGATTACTACAAACATGCAATGTTCCCATTAGAAAAAATTGACTc                                                                                         >  6:229006/1‑149 (MQ=255)
                                                             cGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCAcc                                                                                      >  2:222289/1‑149 (MQ=255)
                                                                 aaGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCtt                                                                                   >  5:142156/1‑148 (MQ=255)
                                                                   gCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTaa                                                                                <  2:235731/149‑1 (MQ=255)
                                                                    cACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTaaa                                                                               <  2:96775/149‑1 (MQ=255)
                                                                       tctcGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGAt                                                                            >  7:310489/1‑149 (MQ=255)
                                                                            gATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGtttt                                                                       <  1:21373/149‑1 (MQ=255)
                                                                              ttCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGtttttt                                                                     <  8:257177/149‑1 (MQ=255)
                                                                                ccTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTc                                                                    <  6:142156/148‑1 (MQ=255)
                                                                                 cTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTAc                                                                                                            <  8:333087/107‑1 (MQ=255)
                                                                                 cTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTAc                                                                                                            >  7:333087/1‑107 (MQ=255)
                                                                                  ttACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTa                                                                                 <  8:185588/133‑1 (MQ=255)
                                                                                  ttACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTa                                                                                 >  7:185588/1‑133 (MQ=255)
                                                                                     ccGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATgc                                                              <  6:344274/149‑1 (MQ=255)
                                                                                     ccGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATgc                                                              <  1:189263/149‑1 (MQ=255)
                                                                                      ccaaTCCAACTTAAGAAGTAAACTGGCAAGGTAATGTCATTTGACAAAATAGAGCTTTACGACATTATTTCACTGCGGATTACTACAAAAAAGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTATTTTCTAAAgcg                                                             >  1:35182/3‑149 (MQ=255)
                                                                                      cGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGGACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATgcg                                                             >  1:79757/1‑149 (MQ=255)
                                                                                      cGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGGACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATgcg                                                             >  1:40839/1‑149 (MQ=255)
                                                                                       gAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATgcgc                                                            <  3:30539/149‑1 (MQ=255)
                                                                                       gAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATgcgc                                                            <  5:335664/149‑1 (MQ=255)
                                                                                            cAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTTCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGGGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGaa                                                       <  5:38062/149‑1 (MQ=255)
                                                                                            cAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGaaaa                                                                                                  <  1:248652/106‑1 (MQ=255)
                                                                                            cAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGaaaa                                                                                                  >  2:248652/1‑106 (MQ=255)
                                                                                                   tgttgtAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCAcag                                                <  7:368458/149‑1 (MQ=255)
                                                                                                     ttgtAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCAcag                                                >  6:80051/1‑147 (MQ=255)
                                                                                                          aaCTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAAc                                         <  2:232284/149‑1 (MQ=255)
                                                                                                             tGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACga                                       >  7:309437/1‑148 (MQ=255)
                                                                                                               gCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTcaca                                                 >  4:443/1‑136 (MQ=255)
                                                                                                               gCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTcaca                                                 <  3:443/136‑1 (MQ=255)
                                                                                                               gCAAGGTAATGTCATTAGTCATAATAGTGCATTTGAACATTAATTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACgaa                                      <  1:139088/147‑1 (MQ=255)
                                                                                                                     tAATGTGTTTAGTCATAATTGTGCTTTTGGTCTTTATATCACTGCGGATTAGTAGAAAAATGCAATGTTACCATTAGAATAGAGTGACTCACGGTTTTAAGATGTTTTTTCTAATGCGCATGATGTCACTGCTGTTTCGAATGAAAATc                              <  2:35182/149‑1 (MQ=255)
                                                                                                                     tAATGTCATTAGTCATAATAGTGCTTTTGGACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATc                              <  2:79757/149‑1 (MQ=255)
                                                                                                                     tAATGTCATTAGTCATAATAGTGCTTTTGGACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATc                              <  2:40839/149‑1 (MQ=255)
                                                                                                                      aaTGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATc                              >  6:129897/1‑148 (MQ=255)
                                                                                                                        tGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTAGCATTAGAAAACATTGAATCACACTTTAAAGATGtttttt                                                                     >  5:60176/1‑107 (MQ=255)
                                                                                                                        tGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGaa                           <  6:237657/149‑1 (MQ=255)
                                                                                                                        tGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGaa                           <  1:231852/149‑1 (MQ=255)
                                                                                                                        tGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGaa                           >  3:151244/1‑149 (MQ=255)
                                                                                                                        tGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGa                            >  2:185364/1‑148 (MQ=255)
                                                                                                                        agTCATTAGTCATAATAGAGCTTTTCAACATTATTTCACTGCGGATTACTACAAAAATTCAATGTTACCAATAGAAAACAGTGACTCACCCTTTAAAGATGtttttt                                                                     <  6:60176/106‑1 (MQ=255)
                                                                                                                            aTTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTcac                                                  <  1:14800/122‑1 (MQ=255)
                                                                                                                            aTTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTcac                                                  >  2:14800/1‑122 (MQ=255)
                                                                                                                            aTTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACaa                        >  3:53699/1‑148 (MQ=255)
                                                                                                                            aTTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACaa                        <  3:200598/148‑1 (MQ=255)
                                                                                                                            aTTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACaa                        <  1:272357/148‑1 (MQ=255)
                                                                                                                             ttAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCAt                                                                                                         <  6:73255/66‑1 (MQ=255)
                                                                                                                             ttAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAata                      >  8:367501/1‑149 (MQ=255)
                                                                                                                             ttAGTCATAATAGCGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAata                      <  5:339332/149‑1 (MQ=255)
                                                                                                                                gTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAAtata                    <  1:287769/148‑1 (MQ=255)
                                                                                                                                gTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAAtata                    <  4:331998/148‑1 (MQ=255)
                                                                                                                                 tCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATaca                  >  5:84627/1‑149 (MQ=255)
                                                                                                                                 tCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATaca                  >  8:161731/1‑149 (MQ=255)
                                                                                                                                   ataataGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAata                      <  6:203521/143‑1 (MQ=255)
                                                                                                                                   ataataGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAata                      >  5:203521/1‑143 (MQ=255)
                                                                                                                                    taataGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATacaca                >  8:343926/1‑148 (MQ=255)
                                                                                                                                    taataGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAAAGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGaa                                                       <  4:322065/109‑1 (MQ=255)
                                                                                                                                    taataGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAAAGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGaa                                                       >  3:322065/1‑109 (MQ=255)
                                                                                                                                      ataGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATACACACAg             <  8:310489/149‑1 (MQ=255)
                                                                                                                                        aGTGCTTTTGAACATTATTTCACTGAGGATAACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCCCCCTTTAAAGATGATTTTACTAATTCGAATGAAGTCAACGCTGAAAAGAAAGAAAATCGAACAATAGACAGACAgg            >  6:209764/1‑148 (MQ=255)
                                                                                                                                          tGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATACACACAGGTAt         >  2:236418/1‑149 (MQ=255)
                                                                                                                                             ttttGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCAt                                                          <  4:29755/97‑1 (MQ=255)
                                                                                                                                                  aaCATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATACACACAGGTATTTGAAAt  <  5:233893/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTTACGCTCAGTTCGTCGTCGTAAAGTCATTCAGGGTGATTTAGAAAACCAAATATCATTCCGAAAAGCACTCTCGGATTCCTTACCGAATCCAACTTATGTTGTAAACTGGCAAGGTAATGTCATTAGTCATAATAGTGCTTTTGAACATTATTTCACTGCGGATTACTACAAAAATGCAATGTTACCATTAGAAAACAGTGACTCACCCTTTAAAGATGTTTTTTCTAATGCGCATGAAGTCACAGCAGAAACGAAAGAAAATCGAACAATATACACACAGGTATTTGAAAT  >  NC_000913/2486044‑2486337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: