| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,649,275 | C→T | 5.5% | A1005T (GCA→ACA) | yfhM ← | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,649,275 | 0 | C | T | 5.5% | 229.3 / 2.3 | 72 | A1005T (GCA→ACA) | yfhM | bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (41/27); new base T (2/2); total (43/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01 | |||||||||||
TTCAGTTTTTGCGGTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCAC > NC_000913/2649127‑2649421 | ttCAGTTTTTGCGGTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGc > 2:301678/1‑149 (MQ=255) tttttGCGGTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTcc < 3:298365/149‑1 (MQ=255) tttGCGGTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCa < 1:237269/148‑1 (MQ=255) gTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTgc < 6:278388/149‑1 (MQ=255) gTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTgc < 4:172343/149‑1 (MQ=255) ggTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATg < 6:172900/148‑1 (MQ=255) gTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGAc > 5:359622/1‑149 (MQ=255) atatCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGAGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCAcc > 2:140238/1‑149 (MQ=255) tCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATTTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGt > 3:339856/1‑148 (MQ=255) tCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGATGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGGAATTCACCGt > 5:96028/1‑148 (MQ=255) ccAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCa < 2:26686/129‑1 (MQ=255) ccAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCa > 1:26686/1‑129 (MQ=255) aGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTaa > 1:329474/1‑148 (MQ=255) aGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTaa > 6:153518/1‑148 (MQ=255) gTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATc > 1:80563/1‑149 (MQ=255) aGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGc < 2:329474/148‑1 (MQ=255) cgcgAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACATTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAAt > 8:53538/1‑149 (MQ=255) gcgAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATc < 1:140238/149‑1 (MQ=255) cgAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCg < 3:51694/149‑1 (MQ=255) aGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCgg > 7:32660/1‑148 (MQ=255) tATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgt < 3:46105/149‑1 (MQ=255) aTCGCCACTCCCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTGTCGTTGCTCCCGAAGTCATCTGCCGTCCATGCTTGAGCCATGACGAGCAATTCACAGTTAAAATCGCCAATCGGCAgag > 3:284534/1‑147 (MQ=255) cGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtgt > 6:107620/1‑148 (MQ=255) cGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAgtga > 3:335652/1‑147 (MQ=255) ccACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAAc > 7:95550/1‑149 (MQ=255) aaGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGcc > 6:113031/1‑149 (MQ=255) aaGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGcc > 1:188186/1‑149 (MQ=255) gcgcGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGCGCCTTCGCCCt > 8:281579/1‑149 (MQ=255) ggCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTc > 8:229209/1‑140 (MQ=255) ggCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTc < 7:229209/140‑1 (MQ=255) aTGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGtt < 6:359622/149‑1 (MQ=255) ttcagttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAg > 7:4856/1‑149 (MQ=255) cagttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGc < 7:197008/148‑1 (MQ=255) agttcagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGCGCGt > 5:165575/1‑149 (MQ=255) cagCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTAc < 1:301678/148‑1 (MQ=255) aaTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCgg > 4:99153/1‑147 (MQ=255) aaTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCa < 8:322889/149‑1 (MQ=255) aTCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCTCTGTTCGTTGAGCGTTACCGGCAg > 5:117949/1‑149 (MQ=255) tCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGTCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAgc > 2:146245/1‑149 (MQ=255) tCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGTCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAgc > 2:141198/1‑149 (MQ=255) tCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAgc > 3:21438/1‑149 (MQ=255) ggTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCctg < 5:153518/149‑1 (MQ=255) cGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCctgctg > 2:277974/1‑148 (MQ=255) tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCTCCCTGTTCGTTGATCGTTACCGGCAGCGCCTGCTGCACGACaa > 5:185894/1‑149 (MQ=255) tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACaa > 2:252015/1‑149 (MQ=255) tATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACaa > 1:18905/1‑149 (MQ=255) aTCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACaa > 1:283672/1‑148 (MQ=255) tCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAata > 7:271783/1‑149 (MQ=255) tCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAata > 4:78373/1‑149 (MQ=255) actttactttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTGCGTGGAGCGTTACCGGCAGCGCCTGCTGCACGACAATAtt < 5:113031/149‑1 (MQ=255) actttactttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGAGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATAtt > 1:42339/1‑149 (MQ=255) ctttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGGGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGATCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTg < 2:80563/149‑1 (MQ=255) tttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGa > 2:310989/1‑149 (MQ=255) tttCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCACTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGa > 8:321939/1‑149 (MQ=255) tCGTTGCTACCGATGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTGCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGAt < 6:96028/148‑1 (MQ=255) tCGTTGCTACCGAAGTCATCTGTCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGa < 1:146245/147‑1 (MQ=255) tCGTTGCTACCGAAGTCATCTGTCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGa < 1:141198/147‑1 (MQ=255) gTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGa < 5:107620/149‑1 (MQ=255) gTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGa < 1:277974/149‑1 (MQ=255) tGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGAtt > 6:356344/1‑145 (MQ=255) tGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGAtt < 5:356344/145‑1 (MQ=255) tACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGCCACCGCCTGCTGCACGCCAATATTGACGTGATTGACcggc > 7:250330/1‑149 (MQ=255) ccGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGAAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACcggcgg < 4:335652/149‑1 (MQ=255) ccGAAGTCATCTACCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACcggcgg > 8:241490/1‑149 (MQ=255) cGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGt > 3:198671/1‑149 (MQ=255) cGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGt > 7:223326/1‑149 (MQ=255) cGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGt > 3:203329/1‑149 (MQ=255) aaGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGttt < 1:119476/149‑1 (MQ=255) aaGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGttt < 7:53538/149‑1 (MQ=255) gTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTac < 1:252015/149‑1 (MQ=255) tGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAgc > 6:80437/1‑100 (MQ=255) tGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAgc < 5:80437/100‑1 (MQ=255) tGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACcaccac > 5:38076/1‑149 (MQ=255) | TTCAGTTTTTGCGGTTTATCGGTAAGATTAGTGATATCCAGCGTCAGACGCGAGGTATCGCCACTCGCCATAAAGCGCGGCATGTTCAGTTCAGCAATCACCGGTGCGGCAACTATCACTTTACTTTCGTTGCTACCGAAGTCATCTGCCGTCCAGGCTTGCGCCATGACGCGCAATTCACCGTTAAAATCGCCAATCGGCAGTGTAACCGAGCCTTCGCCCTGTTCGTTGAGCGTTACCGGCAGCGCCTGCTGCACGACAATATTGACGTGATTGACCGGCGGTTTACCACCAC > NC_000913/2649127‑2649421 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |