Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,187,214 A→C 100% D622A (GAC→GCC)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,187,2140AC100.0% 323.7 / NA 88D622A (GAC→GCC) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (47/41);  total (47/41)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAG  >  NC_000913/4187070‑4187356
                                                                                                                                                |                                                                                                                                              
ttGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGccc                                                                                                                                              <  7:234581/147‑1 (MQ=255)
  ggCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGa                                                                                                                                           <  4:125472/148‑1 (MQ=255)
  ggCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGa                                                                                                                                           <  3:174799/148‑1 (MQ=255)
   gcggTGCCATTCTGTGGATGTTTGTACCGAAAGGGATGGCTTACTGCATCTGCTTAGAGGAGCAGGTTCAAAAAGCACTCTCAAAAATGCTGAACAACTGCTACCTGATTCTCGTTCTGAAACCGACCGTTATTTTTGCGGCCCAGATc                                                                                                                                         <  6:15312/146‑1 (MQ=255)
   gCCGGGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGAGCTGGGTAAAAAAGCAAGCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTTAACCCGACCGTAATTTTTGCGGCCCAGAt                                                                                                                                          <  6:87106/148‑1 (MQ=255)
    ccGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCa                                                                                                                                        <  7:313441/149‑1 (MQ=255)
       tGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGt                                                                                                                                     <  7:242622/149‑1 (MQ=255)
         ccATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTa                                                                                                                                    <  3:67621/148‑1 (MQ=255)
                   gatgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCg                                                                                                                         >  7:379060/1‑149 (MQ=255)
                    atgatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGc                                                                                                                        <  4:37486/149‑1 (MQ=255)
                      gatTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGcc                                                                                                                       >  7:284830/1‑148 (MQ=255)
                       atTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCTCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCt                                                                                                                      <  8:130923/148‑1 (MQ=255)
                       atTGTACCGAAAGGTCTGCCTAACTCCATCGTCAACCAGGCGCGGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCt                                                                                                                      <  2:97994/148‑1 (MQ=255)
                       atTGTACAGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAACGCAATCTCCATAATGGTGAACACCTGCTAGCGCATTCTCGGTCTGAAACCGTCCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTGCGCCt                                                                                                                      <  3:142718/148‑1 (MQ=255)
                         tGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATg                                                                                                                   >  8:354493/1‑149 (MQ=255)
                              cGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAgcg                                                                                                              >  6:317714/1‑149 (MQ=255)
                              cGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAgcg                                                                                                              >  2:345235/1‑149 (MQ=255)
                                aaaGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGcgcg                                                                                                            >  3:253850/1‑149 (MQ=255)
                                       tGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACGGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt                                                                                                     >  8:274966/1‑149 (MQ=255)
                                       tGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGt                                                                                                     >  1:187601/1‑149 (MQ=255)
                                         ccTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTg                                                                                                    >  3:109567/1‑148 (MQ=255)
                                         ccTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTg                                                                                                    >  3:298641/1‑148 (MQ=255)
                                          cTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCa                                                                                                  >  8:20968/1‑149 (MQ=255)
                                             aCTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCCGCGCGTTCTGGTGCATCt                                                                                               >  2:116925/1‑149 (MQ=255)
                                               tCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGt                                                                                             >  6:34442/1‑149 (MQ=255)
                                               tCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGt                                                                                             >  8:214771/1‑149 (MQ=255)
                                                 cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGtt                                                                                            <  8:3514/148‑1 (MQ=255)
                                                 cATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTg                                                                                           <  7:51475/149‑1 (MQ=255)
                                                      tCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGAGTTCTGGTGCATCTGTTGGTATc                                                                                      >  2:180695/1‑149 (MQ=255)
                                                        aaCCAGGCGCTGGGTAAAAAAGCAATCTCCGAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGa                                                                                    >  1:210435/1‑149 (MQ=255)
                                                        aaCCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGa                                                                                    <  1:262211/149‑1 (MQ=255)
                                                        aaCCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGa                                                                                    <  1:257085/149‑1 (MQ=255)
                                                        aaCCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGa                                                                                    <  7:42057/149‑1 (MQ=255)
                                                         aCCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGAt                                                                                   >  7:69111/1‑149 (MQ=255)
                                                         aCCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGAt                                                                                   <  8:284830/149‑1 (MQ=255)
                                                           cAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGa                                                                                 >  2:313748/1‑149 (MQ=255)
                                                            aGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGa                                                                                 >  8:324023/1‑148 (MQ=255)
                                                            aGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGAc                                                                                <  8:272366/149‑1 (MQ=255)
                                                             ggCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACa                                                                               <  3:95315/149‑1 (MQ=255)
                                                             ggCGCTGCGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACa                                                                               <  5:33313/149‑1 (MQ=255)
                                                              gcgcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGCACTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCAACTGTTCGTATCGCTGACAt                                                                              >  6:25985/1‑149 (MQ=255)
                                                               cgcTGGGTAAAAACGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg                                                                             >  1:149240/1‑149 (MQ=255)
                                                               cgcTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATg                                                                             <  8:69722/149‑1 (MQ=255)
                                                                    ggTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCa                                                                         <  4:253850/148‑1 (MQ=255)
                                                                    ggTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCa                                                                         <  7:324023/148‑1 (MQ=255)
                                                                        aaaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCAGGTGCATCTGTTGGTATCGATGACATGGTCATccc                                                                     <  8:197204/148‑1 (MQ=255)
                                                                         aaaaGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCgg                                                                   <  4:298641/149‑1 (MQ=255)
                                                                            aGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGgaga                                                                <  8:238409/149‑1 (MQ=255)
                                                                             gCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaa                                                               <  5:135320/149‑1 (MQ=255)
                                                                              cAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGgag                                                                 >  3:14466/1‑146 (MQ=255)
                                                                               aaTCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGagaag                                                              >  4:123608/1‑148 (MQ=255)
                                                                                 tctcCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGaaa                                                           >  6:276931/1‑149 (MQ=255)
                                                                                    ccAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAACCCGCCCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGGGCATCTGTGGGTATCGATGACATGGTCATCACGGAGAAGAAacac                                                        >  6:203241/1‑149 (MQ=255)
                                                                                     cAAAATGCTGAACACCTGCTACGGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGaaa                                                           <  7:274966/145‑1 (MQ=255)
                                                                                      aaaaTGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACg                                                       >  4:76692/1‑148 (MQ=255)
                                                                                       aaaTGCTGAACACATGCTACCGAATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCATATCATGTAAACCGGATTAGCATATGCAGAGCGTTCTGGTGCATCTGTTGGTATCGATGCCATGGGCATCCCGGAGAAGAAacacta                                                      >  6:364722/1‑146 (MQ=255)
                                                                                          tGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatc                                                  <  7:20968/149‑1 (MQ=255)
                                                                                            cggAACAGCTGCGACCGCATGCTCGGTCTGAAACCGTCCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatc                                                  <  8:171708/145‑1 (MQ=255)
                                                                                             tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATGATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAatcatc                                               >  1:260764/1‑149 (MQ=255)
                                                                                             tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGga                                                                  >  5:103877/1‑130 (MQ=255)
                                                                                             tGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGga                                                                  <  6:103877/130‑1 (MQ=255)
                                                                                               aaCACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCAtct                                              <  1:190068/148‑1 (MQ=255)
                                                                                                acacCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTcc                                            <  7:214771/149‑1 (MQ=255)
                                                                                                  acCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTcc                                            >  5:61702/1‑147 (MQ=255)
                                                                                                     tGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATTACATGGTCATACCGGAGAAGAAacac                                                        >  2:196228/1‑132 (MQ=255)
                                                                                                     tGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAgg                                        >  4:172087/1‑148 (MQ=255)
                                                                                                      gCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAgg                                        >  2:363905/1‑147 (MQ=255)
                                                                                                        tACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAg                                         <  3:329330/144‑1 (MQ=255)
                                                                                                        tACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAg                                         >  4:329330/1‑144 (MQ=255)
                                                                                                        tACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcag                                     >  1:188442/1‑148 (MQ=255)
                                                                                                         aCCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCAGAGgcag                                     >  5:350398/1‑147 (MQ=255)
                                                                                                          ccGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaag                                  >  7:251632/1‑149 (MQ=255)
                                                                                                            gCATTCTCGGTCTGAAACCGCCCGTTATTTTTGCGGCCCAGATCATGTACACCGGCGTCGCCGATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaag                                  >  3:235660/1‑147 (MQ=255)
                                                                                                             cATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaa                                   >  5:184179/1‑145 (MQ=255)
                                                                                                             cATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaa                                   <  6:184179/145‑1 (MQ=255)
                                                                                                               ttCTCGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGgcagaagcag                               >  5:131462/1‑147 (MQ=255)
                                                                                                                tctcGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGcagaagcagaag                            >  1:173873/1‑149 (MQ=255)
                                                                                                                  tcGGTCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGtt                          >  2:158253/1‑149 (MQ=255)
                                                                                                                     gTCTGAAACCGACCGTTATTTTTGCGGCCCAGAACATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGAAGACATGGTCATACCGGAGAAGAAACACGAAATCAACTCGGAGCCAGCATCAGAATTTgat                       >  5:298438/1‑147 (MQ=255)
                                                                                                                      tCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGa                                                      >  4:31098/1‑117 (MQ=255)
                                                                                                                      tCTGAAACCGACCGTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGa                                                      <  3:31098/117‑1 (MQ=255)
                                                                                                                              cgaccgTTATTTTTGCGGCACAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAg              >  5:33777/1‑149 (MQ=255)
                                                                                                                               gaccgTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAgg             <  3:123608/149‑1 (MQ=255)
                                                                                                                               gaccgTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAgg             <  8:69111/149‑1 (MQ=255)
                                                                                                                                accgTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGACATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGa            <  2:210435/149‑1 (MQ=255)
                                                                                                                                accgTTATTTTTGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTTCTGAAATTCAGGa            <  1:180695/149‑1 (MQ=255)
                                                                                                                                       tttttGCGGCCCAGCTCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGAAATGGTCATCCCGGAGAAGAAACACGAAATCATATCCGAGTCAGAAGCAGAAGTTGCTGAAATTCCGGAGCAGTTc     >  6:49227/1‑149 (MQ=255)
                                                                                                                                       tttttGCGGCCCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTc     <  5:34442/149‑1 (MQ=255)
                                                                                                                                          ttGCGGCCCAGATCATTTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAg  >  4:28942/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TTGGCCGTGCCATTCTGTGGATGATTGTACCGAAAGGTCTGCCTTACTCCATCGTCAACCAGGCGCTGGGTAAAAAAGCAATCTCCAAAATGCTGAACACCTGCTACCGCATTCTCGGTCTGAAACCGACCGTTATTTTTGCGGACCAGATCATGTACACCGGCTTCGCCTATGCAGCGCGTTCTGGTGCATCTGTTGGTATCGATGACATGGTCATCCCGGAGAAGAAACACGAAATCATCTCCGAGGCAGAAGCAGAAGTTGCTGAAATTCAGGAGCAGTTCCAG  >  NC_000913/4187070‑4187356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑