Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,128,454 Δ1 bp 5.2% coding (616/1536 nt) murJ → putative lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,128,4540C. 5.2% 262.2 / 6.7 77coding (616/1536 nt)murJputative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base C (36/36);  new base . (2/2);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCT  >  NC_000913/1128312‑1128601
                                                                                                                                              |                                                                                                                                                   
gATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCAcc                                                                                                                                               >  8:177519/1‑149 (MQ=255)
 aTTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCt                                                                                                                                              <  7:177519/149‑1 (MQ=255)
     cGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTg                                                                                                                                             >  4:13224/1‑146 (MQ=255)
      ggCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCTCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaag                                                                                                                                          >  6:168018/1‑148 (MQ=255)
       gCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaagaa                                                                                                                                        <  8:328711/149‑1 (MQ=255)
          tttGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaag                                                                                                                                          >  7:97357/1‑144 (MQ=255)
            tGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATc                                                                                                                                    <  7:270350/148‑1 (MQ=255)
            tGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATc                                                                                                                                    <  5:67031/148‑1 (MQ=255)
               tCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCg                                                                                                                                   >  1:216368/1‑146 (MQ=255)
               tCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCg                                                                                                                                   >  2:316210/1‑146 (MQ=255)
               tCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCg                                                                                                                                   <  2:216368/146‑1 (MQ=255)
               tCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCg                                                                                                                                   <  1:316210/146‑1 (MQ=255)
                  aaCACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCTGCGTCCTTCTGCTGGGGTATCAGCTACCGCACCTGAAGAAGATCGGCATGc                                                                                                                             <  5:384111/149‑1 (MQ=255)
                  aaCACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGc                                                                                                                             >  6:32132/1‑149 (MQ=255)
                    cacTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTg                                                                                                                           <  5:168018/149‑1 (MQ=255)
                     acTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTgg                                                                                                                          <  2:163664/149‑1 (MQ=255)
                            aaCATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGcc                                                                                                                   >  6:244136/1‑149 (MQ=255)
                            aaCATCAGCATGATTGGTTTCGCGCTGTTTGAGTCACCGTACTTTAACCCACGGGTGCTGGCGCTGGCGTGGGCTGTTAGGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGc                                                                                                                    <  8:97357/148‑1 (MQ=255)
                             aCATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCcg                                                                                                                  >  3:202351/1‑149 (MQ=255)
                               aTCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCcgcg                                                                                                                >  4:250534/1‑149 (MQ=255)
                               aTCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCcgcg                                                                                                                >  7:405170/1‑149 (MQ=255)
                                tCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCcgcg                                                                                                                >  3:330231/1‑148 (MQ=255)
                                      tGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAAc                                                                                                         <  8:405170/149‑1 (MQ=255)
                                         ttGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTc                                                                                                      <  3:13224/149‑1 (MQ=255)
                                            ggttCGCGCTGGTGGCCGCACCGGACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCa                                                                                                    <  8:180358/146‑1 (MQ=255)
                                            gTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCa                                                                                                    <  6:175483/148‑1 (MQ=255)
                                            gTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCa                                                                                                    <  2:109269/148‑1 (MQ=255)
                                            gTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCAc                                                                                                   >  4:55089/1‑149 (MQ=255)
                                                 gcgcTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGc                                                                                              >  1:98390/1‑149 (MQ=255)
                                                 gcgcTGTTTGCCGCACCGTACTTTAACCCACCGGTGCGGGCGCTGGCGTGGGCTGTTACGGGCGGCGGCGTCCTGCCGCTGGTGTATAAGCTACCGCCCCTGACGAAGCTCGGCAGGCTGGTCCTGCCGCGCATTACCTTCCACGATGc                                                                                              >  6:378652/1‑149 (MQ=255)
                                                            cGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATgc                                                                                   >  7:135361/1‑149 (MQ=255)
                                                              cACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATgcgc                                                                                 <  1:394457/149‑1 (MQ=255)
                                                                 cGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCgtg                                                                              <  2:98390/149‑1 (MQ=255)
                                                                 cGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCgtg                                                                              >  7:35368/1‑149 (MQ=255)
                                                                   tACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCgtggt                                                                            <  4:202351/149‑1 (MQ=255)
                                                                    aCTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGc                                                                                                                    <  4:15351/108‑1 (MQ=255)
                                                                     cTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCgtggtgc                                                                          >  1:353689/1‑147 (MQ=255)
                                                                     cTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGa                                                                          >  1:347961/1‑148 (MQ=255)
                                                                      tttAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGa                                                                          >  8:275603/1‑148 (MQ=255)
                                                                        tAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGaaa                                                                        <  8:135361/148‑1 (MQ=255)
                                                                        tAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGaaa                                                                        >  5:15476/1‑148 (MQ=255)
                                                                         aaCCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAAc                                                                       <  5:226760/148‑1 (MQ=255)
                                                                         aaCCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAAc                                                                       <  8:35368/148‑1 (MQ=255)
                                                                            ccACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCgtggt                                                                            <  8:63182/140‑1 (MQ=255)
                                                                            ccACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGagattcg                                                                    >  7:63182/1‑142 (MQ=255)
                                                                              aCCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATgg                                                                 >  4:130840/1‑149 (MQ=255)
                                                                                       ggcgctggcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGa                                                        >  2:121212/1‑149 (MQ=255)
                                                                                          gctggcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATcc                                                     >  4:226305/1‑149 (MQ=255)
                                                                                            tggcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGa                                                                    <  1:18039/132‑1 (MQ=255)
                                                                                            tggcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGa                                                                    >  2:18039/1‑132 (MQ=255)
                                                                                              gcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTg                                                  <  3:89598/148‑1 (MQ=255)
                                                                                              gcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTg                                                  <  5:244136/148‑1 (MQ=255)
                                                                                              gcgTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTg                                                  <  4:330231/148‑1 (MQ=255)
                                                                                                    gCTGTTACGGTCGGCGGCGTCCTGCAGGTGGGGGAGCCGCTAGCGCGCCTGAAGCAGATCGGCATGCTGGTGCTGCCGCGCAGTAACTTGCGCGATGCCGGAGCAATGCGCGTGGGGAAACAGCTGGGACCGGCGATGCTGGGCGtctc                                           >  4:152487/1‑149 (MQ=255)
                                                                                                        ttACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCtgt                                        <  2:347961/147‑1 (MQ=255)
                                                                                                        ttACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCtgt                                        <  2:353689/147‑1 (MQ=255)
                                                                                                          aCGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCTCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGa                                      <  4:348147/148‑1 (MQ=255)
                                                                                                          aCGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGa                                      <  6:261211/148‑1 (MQ=255)
                                                                                                           cGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGc                                    <  3:250534/149‑1 (MQ=255)
                                                                                                           cGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGc                                    >  3:47188/1‑149 (MQ=255)
                                                                                                            ggTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGc                                    >  3:188068/1‑148 (MQ=255)
                                                                                                              tCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAg                                 <  4:47188/149‑1 (MQ=255)
                                                                                                                ggcggcGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGc                                    >  1:90331/1‑144 (MQ=255)
                                                                                                                 gcggcgTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGAtc                              <  4:188068/149‑1 (MQ=255)
                                                                                                                  cggcgTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCTCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGAtct                             >  8:414894/1‑149 (MQ=255)
                                                                                                                  cggcgTCCTGCAGCTGGGGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACTATGCCGGAGCAATGCGCGTGGTGAAACAGATGTGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGAtct                             <  5:32132/149‑1 (MQ=255)
                                                                                                                      gTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCAACGATGCCGGAGCAATGCTCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCt                          >  3:194619/1‑148 (MQ=255)
                                                                                                                         cTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATTGGACCCTCGATCCTTGGCGTCTCTCTGAGCCAGATCTCCTTAat                      >  6:105479/1‑149 (MQ=255)
                                                                                                                             aGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAatcatc                  <  3:130840/149‑1 (MQ=255)
                                                                                                                                    gtATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGAt                                                                   >  6:381937/1‑93 (MQ=255)
                                                                                                                                    gtATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGAt                                                                   <  5:381937/93‑1 (MQ=255)
                                                                                                                                       tCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCAttt        >  8:371098/1‑149 (MQ=255)
                                                                                                                                         aGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAatcatc                  <  7:271556/137‑1 (MQ=255)
                                                                                                                                         aGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAatcatc                  >  8:271556/1‑137 (MQ=255)
                                                                                                                                         aGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCAttttt      >  8:168776/1‑149 (MQ=255)
                                                                                                                                          gCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTg     >  2:243654/1‑149 (MQ=255)
                                                                                                                                             aCCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCt  >  4:233207/1‑149 (MQ=255)
                                                                                                                                              ccGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCt  <  3:226305/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCATGCTGGTCCTGCCGCGCATTAACTTCCACGATGCCGGAGCAATGCGCGTGGTGAAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCT  >  NC_000913/1128312‑1128601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: