Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,601,507 G→T 5.1% R194S (CGT→AGT)  ftsX ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,601,5070GT 5.1% 314.6 / 3.7 92R194S (CGT→AGT) ftsXputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (44/43);  new base T (3/2);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

TGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGA  >  NC_000913/3601360‑3601655
                                                                                                                                                   |                                                                                                                                                    
tGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGa                                                                                                                                                     >  8:185226/1‑149 (MQ=255)
  ttACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGa                                                                                                                                                     <  1:409185/147‑1 (MQ=255)
  ttACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGAt                                                                                                                                                    <  5:106102/148‑1 (MQ=255)
  ttACAGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATc                                                                                                                                                   <  2:201997/149‑1 (MQ=255)
    aCCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCAc                                                                                                                                                 >  4:143484/1‑149 (MQ=255)
           aCGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCg                                                                                                                                           >  4:314134/1‑148 (MQ=255)
             gAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGCCGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAAAGCCGTTAATCTGCGTGATACGATCACGCAGCGTAt                                                                                                                                        >  1:149103/1‑149 (MQ=255)
              aGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGTCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAACCTGCGTGATACGATCACGCAGCGTAtt                                                                                                                                       <  3:126742/149‑1 (MQ=255)
                 aaCACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAg                                                                                                                                    <  6:173161/149‑1 (MQ=255)
                      ggCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGAtt                                                                                                                               <  4:137316/149‑1 (MQ=255)
                        ccgccACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCAGGTCAACGCCGACAGACGGTCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTcc                                                                                                                             >  6:191869/1‑149 (MQ=255)
                           ccaccaTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTc                                                                                                                          >  3:312822/1‑149 (MQ=255)
                              ccaTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCACACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTcccc                                                                                                                       >  4:101160/1‑149 (MQ=255)
                               caTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCATACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCt                                                                                                                      >  5:42604/1‑149 (MQ=255)
                                aTCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                     >  3:230943/1‑149 (MQ=255)
                                    acacGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTc                                                                                                                                      <  5:251622/128‑1 (MQ=255)
                                    acacGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTc                                                                                                                                      >  6:251622/1‑128 (MQ=255)
                                     cacGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGTGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAAt                                                                                                                <  5:292825/149‑1 (MQ=255)
                                     cacGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAAt                                                                                                                >  3:74204/1‑149 (MQ=255)
                                      acGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCATACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATc                                                                                                               <  3:101160/149‑1 (MQ=255)
                                             tcatcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTc                                                                                                        <  2:140909/149‑1 (MQ=255)
                                              catcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCg                                                                                                       >  7:263559/1‑149 (MQ=255)
                                              catcGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCg                                                                                                       >  3:181158/1‑149 (MQ=255)
                                                  gCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGCTACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGAt                                                                                                   >  1:325968/1‑149 (MQ=255)
                                                   ccGATACGCGCCCGAACAGCCCGGTCAACGCCGCCAGACGGGCAAACCTGCGGTCATCCTTCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCAAGCAGCGTATTCAGTGATTCCTTCCCCTGGAAATCGAGTTTCGGGATc                                                                                                  <  6:12969/149‑1 (MQ=255)
                                                   ccGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATc                                                                                                  <  7:185226/149‑1 (MQ=255)
                                                   ccGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACAAGCTGTCATACATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGCAt                                                                                                   >  4:88425/1‑148 (MQ=255)
                                                      aaaCGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCg                                                                                                       >  3:15161/1‑141 (MQ=255)
                                                      aaaCGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCg                                                                                                       <  4:15161/141‑1 (MQ=255)
                                                             cccGAACAGCCCGGTCAACGCCGACAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTTGAAATCGAGTTTCGGGATCACAACCGCCa                                                                                        >  6:56003/1‑149 (MQ=255)
                                                                 aCCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGc                                                                                    <  4:312822/149‑1 (MQ=255)
                                                                  ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGcc                                                                                   >  1:191529/1‑149 (MQ=255)
                                                                  ccAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGcc                                                                                   >  1:27525/1‑149 (MQ=255)
                                                                   cAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATca                                                                                                 <  7:174635/134‑1 (MQ=255)
                                                                   cAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATca                                                                                                 >  8:174635/1‑134 (MQ=255)
                                                                     gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCgg                                                                                                             >  8:203027/1‑119 (MQ=255)
                                                                     gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCgg                                                                                                             <  7:203027/120‑2 (MQ=255)
                                                                     gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATca                                                                                                 >  7:129698/1‑132 (MQ=255)
                                                                     gCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATca                                                                                                 <  8:129698/132‑1 (MQ=255)
                                                                        cGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                     <  3:35049/109‑1 (MQ=255)
                                                                        cGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTg                                                                                                                     >  4:35049/1‑109 (MQ=255)
                                                                        cGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGc                                                                             <  3:314134/149‑1 (MQ=255)
                                                                         ggTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCg                                                                            >  7:116132/1‑149 (MQ=255)
                                                                             aaCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCCCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGtt                                                                        >  1:376133/1‑149 (MQ=255)
                                                                             aaCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGa                                                                                                    >  4:358777/1‑121 (MQ=255)
                                                                             aaCGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGa                                                                                                    <  3:358777/121‑1 (MQ=255)
                                                                                gccgccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttc                                                                     <  4:230943/149‑1 (MQ=255)
                                                                                gccgccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttc                                                                     <  8:263559/149‑1 (MQ=255)
                                                                                   gccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCgg                                                                           >  6:244293/1‑140 (MQ=255)
                                                                                   gccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCgg                                                                           <  5:244293/140‑1 (MQ=255)
                                                                                    ccAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTcc                                                                 <  3:143484/149‑1 (MQ=255)
                                                                                      aGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCgg                                                                                                      <  7:109298/110‑1 (MQ=255)
                                                                                      aGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCgg                                                                                                      >  8:109298/1‑110 (MQ=255)
                                                                                       gACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCATTGCAGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGc                                                              <  2:149103/149‑1 (MQ=255)
                                                                                          gggCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata                                                           >  1:25898/1‑149 (MQ=255)
                                                                                            gCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATc                                                         >  1:364379/1‑149 (MQ=255)
                                                                                              ataCCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCa                                                       <  6:42604/147‑1 (MQ=255)
                                                                                                   aGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGTGTTTTCTTCCAGAATATCCAGCGca                                                  <  2:325968/149‑1 (MQ=255)
                                                                                                       gTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata                                                           >  5:183745/1‑136 (MQ=255)
                                                                                                       gTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCata                                                           <  6:183745/136‑1 (MQ=255)
                                                                                                        tCATCCATCCTCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCaccacc                                             <  5:216157/149‑1 (MQ=255)
                                                                                                        tCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                              >  1:187834/1‑148 (MQ=255)
                                                                                                           tccatccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCaa                                           >  8:70298/1‑148 (MQ=255)
                                                                                                               tccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                     >  1:89644/1‑86 (MQ=255)
                                                                                                               tccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                     <  2:89644/86‑1 (MQ=255)
                                                                                                               tccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAac                                        >  6:154648/1‑147 (MQ=255)
                                                                                                                ccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                     >  2:193685/1‑85 (MQ=255)
                                                                                                                ccGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCggg                                                                                                     <  1:193685/85‑1 (MQ=255)
                                                                                                                   cACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccagacc                                  <  2:364379/149‑1 (MQ=255)
                                                                                                                      ttCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAg                                                                              <  4:314555/102‑1 (MQ=255)
                                                                                                                         gTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTAGGGATCACCACCGCCACTGCAGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTAc                            <  5:191869/149‑1 (MQ=255)
                                                                                                                         cccaccgCCGTTAATCTGCGCGACACGATCACGCAGCGTATTCAGTGAATCCGTCCCCTGGAAATCGAGCTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCaccacc                                             <  4:365702/126‑1 (MQ=255)
                                                                                                                            aaTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                              >  5:187801/1‑128 (MQ=255)
                                                                                                                            aaTGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGcaccac                                              <  6:187801/128‑1 (MQ=255)
                                                                                                                                 cGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCa                    >  6:230533/1‑149 (MQ=255)
                                                                                                                                  gTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttc                                                                     >  5:363368/1‑99 (MQ=255)
                                                                                                                                  gTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTttc                                                                     <  6:363368/99‑1 (MQ=255)
                                                                                                                                  gTTAATCTGCGTGATACGATCAAGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCAATGCCGGAAGCGGGTTTTCATACAGCATATCCAGCGAACCACCAAAACCAGCCCAGTTACGGAACTCAc                   >  8:389981/1‑149 (MQ=255)
                                                                                                                                       gcTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAacca                                      <  8:50218/124‑1 (MQ=255)
                                                                                                                                       gcTGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAacca                                      >  7:50218/2‑125 (MQ=255)
                                                                                                                                         tGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccaga                                    >  2:72497/1‑125 (MQ=255)
                                                                                                                                         tGCGTGATCGTATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAaccaga                                    <  1:72497/125‑1 (MQ=255)
                                                                                                                                         tGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGc            >  8:38167/1‑149 (MQ=255)
                                                                                                                                           cGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGt          <  5:230533/149‑1 (MQ=255)
                                                                                                                                            gTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCAcc                  <  5:162914/140‑1 (MQ=255)
                                                                                                                                            gTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCAcc                  >  6:162914/1‑140 (MQ=255)
                                                                                                                                              gATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCtt       >  1:246032/1‑149 (MQ=255)
                                                                                                                                               aTACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAac                                        >  1:118510/1‑115 (MQ=255)
                                                                                                                                               aTACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAac                                        <  2:118510/115‑1 (MQ=255)
                                                                                                                                               aTACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCtt       <  2:191529/148‑1 (MQ=255)
                                                                                                                                                 aCGATCCCGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCAc    >  8:338474/1‑149 (MQ=255)
                                                                                                                                                  cGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCAc    <  7:70298/148‑1 (MQ=255)
                                                                                                                                                   gATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCAc    >  2:19112/1‑147 (MQ=255)
                                                                                                                                                   gATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACga  <  7:38167/149‑1 (MQ=255)
                                                                                                                                                   gATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACga  <  2:246032/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGTTACCGATGACGAGGAACACGGCCGCCACCATCAACACGCCGATCATCGCCGAAACGCGCCCGACCAGCCCGGTCAACGCCGCCAGACGGGCAAACCAGCTGTCATCCATCCGCACTTCGTCAATGCCGTTAATCTGCGTGATACGATCACGCAGCGTATTCAGTGATTCCGTCCCCTGGAAATCGAGTTTCGGGATCACCACCGCCACTGCCGGAAGCGGGTTTTCTTCCAGCATATCCAGCGCACCACCAAAACCAGACCAGTTACGGAACTCACCCAGTGCGTCTTCACGA  >  NC_000913/3601360‑3601655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: