Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,137,911 A→T 5.4% intergenic (‑254/+21) yijF ← / ← gldA DUF1287 family protein/glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,137,9110AT 5.4% 403.1 / 7.4 126intergenic (‑254/+21)yijF/gldADUF1287 family protein/glycerol dehydrogenase, NAD+ dependent; 1,2‑propanediol:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (53/66);  new base T (4/3);  total (57/69)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 132 total aligned reads displayed.

GTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTG  >  NC_000913/4137766‑4138059
                                                                                                                                                 |                                                                                                                                                    
gTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGc                                                                                                                                                   >  8:40563/1‑149 (MQ=255)
 tttCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTTCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATAAGGCAATTTTGCGTTCAAACTCCCGGACAAGcc                                                                                                                                                  >  5:276189/1‑149 (MQ=255)
 ttacacaTTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGACTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGcc                                                                                                                                                  >  6:341488/7‑149 (MQ=255)
    cAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCgg                                                                                                                                                <  7:377577/148‑1 (MQ=255)
        ttACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGtt                                                                                                                                           <  3:107972/149‑1 (MQ=255)
        ttACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGtt                                                                                                                                           <  2:316574/149‑1 (MQ=255)
         tACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGttt                                                                                                                                          >  4:342082/1‑149 (MQ=255)
           cTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTgg                                                                                                                                        <  8:352407/149‑1 (MQ=255)
           cTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                         <  5:123650/148‑1 (MQ=255)
            tGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                         >  1:72439/1‑147 (MQ=255)
              gCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCTCGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGATCAAACTCCCGGACAAGCCGGGAGTTTGGa                                                                                                                                       <  7:376315/147‑1 (MQ=255)
              gCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTgaa                                                                                                                                       <  5:178026/147‑3 (MQ=255)
               cacaGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTa                                                                                                                                    <  8:160042/149‑1 (MQ=255)
                 caGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAgg                                                                                                                                  <  7:40563/149‑1 (MQ=255)
                  agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                         <  2:153770/141‑1 (MQ=255)
                  agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                         >  1:153770/1‑141 (MQ=255)
                  agagTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGt                                                                                                                                 >  7:301173/1‑149 (MQ=255)
                    agTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTa                                                                                                                               <  5:201643/149‑1 (MQ=255)
                    agTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTa                                                                                                                               >  7:258876/1‑149 (MQ=255)
                      tGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                         >  6:343278/1‑137 (MQ=255)
                      tGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTg                                                                                                                                         <  5:343278/137‑1 (MQ=255)
                       gCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTc                                                                                                                            <  2:145789/149‑1 (MQ=255)
                        ccGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTcc                                                                                                                           >  6:236008/1‑149 (MQ=255)
                        ccGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATAAGGAAATTTTTCGTTCAAACTCCCTGACAAGCCGGGAGTTTGTAGTAGGTTATTc                                                                                                                            >  6:230176/1‑148 (MQ=21)
                        ccGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCCCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGACTAGGTTATTc                                                                                                                            >  3:158514/1‑148 (MQ=255)
                            aTGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCAct                                                                                                                       <  7:147031/149‑1 (MQ=255)
                             tGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCActc                                                                                                                      <  7:281858/149‑1 (MQ=255)
                                 gAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATTTTGCAGAGATTGCGTAGTCCTGATAAGCGTAGGGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTg                                                                                                                   >  6:342216/1‑148 (MQ=255)
                                 gAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATACGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGc                                                                                                                  <  5:236008/149‑1 (MQ=255)
                                  aGTTAACGCCTTATACTGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGc                                                                                                                  <  5:230176/148‑1 (MQ=255)
                                  aGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCa                                                                                                                 <  8:166233/149‑1 (MQ=255)
                                  aGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCa                                                                                                                 <  3:41515/149‑1 (MQ=255)
                                    tGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAg                                                                                                                <  6:145082/148‑1 (MQ=255)
                                    tGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAg                                                                                                                >  3:207814/1‑148 (MQ=255)
                                        cGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAAc                                                                                                           >  1:51671/1‑149 (MQ=255)
                                         gCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACg                                                                                                          <  5:38011/149‑1 (MQ=255)
                                           cTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt                                                                                                        >  6:125391/1‑149 (MQ=255)
                                           cTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCt                                                                                                        >  5:182891/1‑149 (MQ=255)
                                                  ggCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTCCAAACTCCCGGCTTGTCCGGGAGTTTgaac                                                                                                                                      >  6:211911/1‑109 (MQ=17)
                                                  ggCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTCCAAACTCCCGGCTTGTCCGGGAGTTTgaac                                                                                                                                      <  5:211911/112‑4 (MQ=17)
                                                  ggCCTACAAAAGCAAGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTa                                                                                                 <  1:43931/149‑1 (MQ=255)
                                                     cTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTg                                                                                              >  8:307269/1‑149 (MQ=255)
                                                      tACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTAt                                                                                                                              <  8:401985/116‑1 (MQ=255)
                                                      tACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTAt                                                                                                                              >  7:401985/1‑116 (MQ=255)
                                                         aaaaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGc                                                                                                                  >  2:337110/1‑125 (MQ=255)
                                                         aaaaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGc                                                                                                                  <  1:337110/125‑1 (MQ=255)
                                                         aaaaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCg                                                                                          >  4:237485/1‑149 (MQ=255)
                                                           aaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGc                                                                                        <  8:373918/149‑1 (MQ=255)
                                                           aaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGc                                                                                        <  4:63099/149‑1 (MQ=255)
                                                           aaGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGc                                                                                        <  8:114772/149‑1 (MQ=255)
                                                            aGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCt                                                                                       <  8:154875/149‑1 (MQ=255)
                                                                 gCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcag                                                                                  <  8:258876/149‑1 (MQ=255)
                                                                     aTTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGAGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACcagca                                                                                >  3:100000/1‑147 (MQ=255)
                                                                       tCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgc                                                                            >  2:69895/1‑149 (MQ=255)
                                                                          atatatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgcggc                                                                         <  4:158514/149‑1 (MQ=255)
                                                                          atatatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAgcggc                                                                         <  6:276189/149‑1 (MQ=255)
                                                                             tatTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAg                                                                                                                                      <  6:1767/85‑1 (MQ=255)
                                                                                 gCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAAc                                                                   <  6:182891/148‑1 (MQ=255)
                                                                                  cAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCg                                                                                          >  6:53768/1‑124 (MQ=255)
                                                                                  cAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCg                                                                                          <  5:53768/124‑1 (MQ=255)
                                                                                  cAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCt                                                                 <  5:383549/149‑1 (MQ=255)
                                                                                   agagATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCt                                                                 >  5:174847/1‑148 (MQ=255)
                                                                                     agaTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGAt                                                              >  6:46174/1‑149 (MQ=255)
                                                                                       aTTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCt                                                            <  2:390573/149‑1 (MQ=255)
                                                                                           cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGACgg                                                                                         >  6:319153/1‑116 (MQ=255)
                                                                                           cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGACgg                                                                                         <  5:319153/116‑1 (MQ=255)
                                                                                             tAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTc                                                      <  4:280736/149‑1 (MQ=255)
                                                                                               ggCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGTTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTcgc                                                    <  2:330478/149‑1 (MQ=255)
                                                                                                gCCTGATAAGCGTAGCGCATCAGGCAAGTTTGCGTTCAAACTCCCCGACAAGCCGGGAGTTTGGAGTAGGTTATGCCCACGCTTGCAGGAAACGCTGCCCGTACTGGTCGGCGACCAGCAGAGCGGCGTACACCTGATCTGGCGTcgcg                                                   >  5:77235/1‑149 (MQ=255)
                                                                                                 ccTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTg                                                           <  2:223904/140‑1 (MQ=255)
                                                                                                 ccTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTg                                                           >  1:223904/1‑140 (MQ=255)
                                                                                                     aTAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTAGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCgccgcc                                              >  4:373989/1‑149 (MQ=255)
                                                                                                        aGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAg                                            <  4:207814/148‑1 (MQ=255)
                                                                                                        aGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAg                                            <  3:342082/148‑1 (MQ=255)
                                                                                                           gTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAt                                        <  6:312653/149‑1 (MQ=255)
                                                                                                           gTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAt                                        >  5:312653/1‑149 (MQ=255)
                                                                                                                gcATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCAtgttgt                                   <  2:51671/149‑1 (MQ=255)
                                                                                                                  aTCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgcc                                                 <  7:403616/133‑1 (MQ=255)
                                                                                                                  aTCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGcgcc                                                 >  8:403616/1‑133 (MQ=255)
                                                                                                                     aGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAAt                               <  1:217852/148‑1 (MQ=255)
                                                                                                                     aGGCAATTTTGCGCTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATg                              <  5:8472/149‑1 (MQ=255)
                                                                                                                       gCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATg                              <  1:259953/147‑1 (MQ=255)
                                                                                                                       gCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATg                              >  2:259953/1‑147 (MQ=255)
                                                                                                                          aTTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTc                         <  5:125391/149‑1 (MQ=255)
                                                                                                                          aTTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTc                         <  5:173301/149‑1 (MQ=255)
                                                                                                                               tactccAAACTCCCGGCTTGTCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCCGCAGCGAGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCtt                    >  6:396279/6‑149 (MQ=255)
                                                                                                                                  ttCAAACTCCCGGACAAGCCGTGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTg                 <  4:100000/149‑1 (MQ=255)
                                                                                                                                  ttCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTACACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGGTTCACCTTCTg                 >  3:229715/1‑149 (MQ=255)
                                                                                                                                     aaaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGca               >  8:364097/1‑148 (MQ=255)
                                                                                                                                     aaaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTAATGGTCGGCTACCAGCAGATCGGCGTAAACCTAATCATGCGTCGAGCCGCCAGTCATGATGTGAATGGTATCACCTTCTGca               >  8:257587/1‑148 (MQ=255)
                                                                                                                                      aaCTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTTTTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGACGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGgaca             >  5:143103/1‑149 (MQ=255)
                                                                                                                                        cTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATg           <  5:342216/149‑1 (MQ=255)
                                                                                                                                         tCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGc          >  7:150448/1‑149 (MQ=255)
                                                                                                                                          cccGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGcc         >  7:119574/1‑149 (MQ=255)
                                                                                                                                           ccGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGtt                           >  4:139643/1‑130 (MQ=255)
                                                                                                                                           ccGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGtt                           <  3:139643/130‑1 (MQ=255)
                                                                                                                                              gACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCtt     >  2:224232/1‑149 (MQ=255)
                                                                                                                                                 aaGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTg                                                           <  7:173832/92‑1 (MQ=255)
                                                                                                                                                 aaGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCa                                             <  6:139054/106‑1 (MQ=255)
                                                                                                                                                 aaGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTg  <  6:174847/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTTTCAGATTACTGGCACAGAGTGCCGGATGCGGAGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAATTCAATATATTGCAGAGATTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTTTGCGTTCAAACTCCCGGACAAGCCGGGAGTTTGGAGTAGGTTATTCCCACTCTTGCAGGAAACGCTGACCGTACTGGTCGGCTACCAGCAGAGCGGCGTAAACCTGATCTGGCGTCGCGCCGCCAGGCATGTTGTGAATGGTTTCACCTTCTGCACATGCCGCTTCTG  >  NC_000913/4137766‑4138059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: