| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,606,239 | A→G | 14.0% | noncoding (48/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,239 | 0 | A | G | 14.0% | 63.7 / 4.4 | 29 | noncoding (48/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base A (12/12); new base G (2/2); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGA > NC_000913/4606093‑4606355 | ggATTTGAACCCCCACGTCCGTAAGGACATTAACTCCTGCAGCTAGCGCGTATACCAATTCCGCGACCTTATCACATTCATCTTACTTTTTTTGATCTCTCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAac < 4:187352/149‑1 (MQ=25) tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa > 6:31097/1‑118 (MQ=255) tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGaa < 5:31097/118‑1 (MQ=255) acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa > 3:21828/1‑122 (MQ=255) acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 4:21828/122‑1 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAacac > 3:342673/1‑99 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAacac < 4:342673/99‑1 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc < 2:161958/112‑1 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc > 1:161958/1‑112 (MQ=255) ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGACTCGTTTGGTGCGAGGGGGGGGACGTGCCCCCCCACCGCCGTAAGAACACTAACACATGAAGATAGCGAGTCTACCAATTGCGCCACCTTCGCCCAGGGCGAGCaa > 2:89609/1‑149 (MQ=25) gCCACCTTCGCACAGTCATCTAAATTATTTAGAAATCGCGTCGTTTGGTGCTAGGGTGGTCACTTGCGCCCACAAGTCCGTAAGAACACTAACACATGAATCTAGAGCGTCTAGGAATTCCGCCCCCTTCGCGCGGTGCGAGCAatag > 2:95445/1‑147 (MQ=255) gCCACCTTCGCACAGTCATCATACTTTATTTTATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAat < 1:95445/146‑1 (MQ=21) aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGa < 7:134814/138‑1 (MQ=255) aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGACACCTTCGCCCAGTGCGa > 8:134814/1‑138 (MQ=255) tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc > 5:109866/1‑106 (MQ=255) tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc < 6:109866/106‑1 (MQ=255) atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc > 4:232450/1‑87 (MQ=255) atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc < 3:232450/87‑1 (MQ=255) atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg < 7:397089/128‑1 (MQ=255) atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg > 8:397089/1‑128 (MQ=255) gCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACTCATAAAGCAAGCGAGTCAACCAATTACGACACCTTCGCACAGTGCGAGCAATATCCACGTGGTTTATGATGCGAGGGGGGGGCCTTGGAccc > 6:147597/1‑149 (MQ=18) ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca > 7:404547/1‑44 (MQ=38) ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAaca < 8:404547/44‑1 (MQ=38) aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCCGAAGCTAGCGCGTCGACCAATTCCGCCCCCTTCGCCCAGTGCGAGCAATATCAACGTGTGTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGa < 5:147597/149‑1 (MQ=25) gAACCCCCACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc < 4:361798/74‑1 (MQ=255) ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc < 5:283770/70‑1 (MQ=18) ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc > 6:283770/1‑70 (MQ=18) gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg < 4:235286/121‑1 (MQ=255) gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAgg > 3:235286/1‑121 (MQ=255) aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg > 7:300710/1‑59 (MQ=255) aaGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg < 8:300710/59‑1 (MQ=255) | GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGA > NC_000913/4606093‑4606355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |