Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,063,041 G→C 6.0% S245R (AGC→AGG) ‡ cbpA ← DnaK co‑chaperone; curved DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,063,0410GC 6.0% 345.3 / 10.7 100S245R (AGC→AGG) ‡cbpADnaK co‑chaperone; curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (42/52);  new base C (3/3);  total (45/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

TGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATAT  >  NC_000913/1062895‑1063186
                                                                                                                                                  |                                                                                                                                                 
tGGGCGTCTGCCAGTTGCTGCCACAGAGCGGCTGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCAGTTTTTTGCTAAACAGACCTTTGCCTTTAACGCGCAATCATTGCCCGGCCTGGCt                                                                                                                                                 >  1:352956/1‑149 (MQ=255)
  ggCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGc                                                                                                                                               >  3:134051/1‑149 (MQ=255)
   gCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGcc                                                                                                                                              >  3:176178/1‑149 (MQ=255)
     gTCTGCCCGTTGCTGCCTGAGCGCGGCAGTGTTTTCTTCGGGGTTCGGCGGCATGACGATTTTGAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCTGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTg                                                                                                                                            <  3:254319/149‑1 (MQ=255)
         gCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCgg                                                                                                                                        >  8:229794/1‑149 (MQ=255)
          ccAGTTGCTGCCACAGCGCGGCTGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCGTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCggg                                                                                                                                       >  2:215822/1‑149 (MQ=255)
          ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGc                                                                                                                                          <  3:227486/146‑1 (MQ=255)
          ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCggg                                                                                                                                       <  2:361649/149‑1 (MQ=255)
          ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCgg                                                                                                                                        >  1:15228/1‑148 (MQ=255)
          ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGGCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCgg                                                                                                                                        <  5:237802/148‑1 (MQ=255)
           cAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGa                                                                                                                                      <  4:121570/149‑1 (MQ=255)
                 ctgcCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGt                                                                                                                                  >  4:177635/1‑147 (MQ=255)
                  tgcCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAg                                                                                                                               >  3:339070/1‑149 (MQ=255)
                    ccACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAg                                                                                                                               <  7:104396/147‑1 (MQ=255)
                    ccACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGc                                                                                                                              >  8:44826/1‑148 (MQ=255)
                    ccACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGc                                                                                                                              >  2:408440/1‑148 (MQ=255)
                     cacaGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCa                                                                                                                             >  1:401936/1‑148 (MQ=255)
                       caGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCaaaa                                                                                                                          <  7:44826/149‑1 (MQ=255)
                          cgcgGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGc                                                                                                                       <  4:348248/149‑1 (MQ=255)
                               cAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttct                                                                                                                  <  5:243771/149‑1 (MQ=255)
                                aGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttctt                                                                                                                 <  2:356975/149‑1 (MQ=255)
                                aGTGGTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCGGCCTGGCGGGATAGTCAGCAAAATGctttctt                                                                                                                 <  1:325696/149‑1 (MQ=255)
                                 gtgtTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttcttt                                                                                                                <  3:177635/149‑1 (MQ=255)
                                    ttttCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCa                                                                                                              <  2:286488/148‑1 (MQ=255)
                                         aTCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTg                                                                                                        <  4:176178/149‑1 (MQ=255)
                                         aTCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTg                                                                                                        <  7:229794/149‑1 (MQ=255)
                                            cGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGaa                                                                                                     >  1:376967/1‑149 (MQ=255)
                                                 tCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                 <  5:33757/148‑1 (MQ=255)
                                                 tCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTAAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                 <  1:7639/148‑1 (MQ=255)
                                                       gcATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTa                                                                                          <  2:9003/149‑1 (MQ=255)
                                                          tCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATGGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTa                                                                                          >  8:20331/1‑146 (MQ=255)
                                                          tCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATGGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTa                                                                                          <  7:20331/146‑1 (MQ=255)
                                                          tCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCa                                                                                       >  1:191467/1‑149 (MQ=255)
                                                            aCGATTTTCAGTTCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCACAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                 <  2:85748/137‑1 (MQ=255)
                                                            aCGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCACAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                 >  1:85748/1‑137 (MQ=255)
                                                            aCGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACttt                                                                                           <  6:214194/143‑1 (MQ=255)
                                                            aCGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACttt                                                                                           >  5:214194/1‑143 (MQ=255)
                                                                ttttCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                 <  5:231826/133‑1 (MQ=255)
                                                                ttttCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                 >  6:231826/1‑133 (MQ=255)
                                                                   tCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAgcgc                                                                               <  1:408440/148‑1 (MQ=255)
                                                                     aGTACCGCATACAGATCGCCGGGCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGcgccg                                                                             <  5:340433/148‑1 (MQ=255)
                                                                      gTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCt                                                                           >  1:148557/1‑149 (MQ=255)
                                                                         ccGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTcc                                                                        >  3:281879/1‑149 (MQ=255)
                                                                           gCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCa                                                                      <  2:191467/149‑1 (MQ=255)
                                                                            cATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                            <  7:235267/110‑1 (MQ=255)
                                                                            cATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                            >  8:235267/1‑110 (MQ=255)
                                                                              tACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACgg                                                                   <  2:376967/149‑1 (MQ=255)
                                                                               aCAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACgg                                                                   <  4:134051/148‑1 (MQ=255)
                                                                                cAGTTCGCCGGTCTTTATTATGCTCACCAGACCTTTGCCTTTAACGCGCAATCATTGCCCGGCCTGGCTGCCTGGCGGGATTGTCAGCAATATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACggg                                                                  <  2:352956/148‑1 (MQ=255)
                                                                                cAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACggg                                                                  <  2:401936/148‑1 (MQ=255)
                                                                                 aGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCt                                                                <  5:379643/149‑1 (MQ=255)
                                                                                   aTCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACg                                                                    >  6:201084/1‑143 (MQ=255)
                                                                                   aTCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACg                                                                    <  5:201084/143‑1 (MQ=255)
                                                                                   aTCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTaa                                                              >  7:261453/1‑149 (MQ=255)
                                                                                        cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                            <  2:383635/98‑1 (MQ=255)
                                                                                        cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                            >  1:383635/1‑98 (MQ=255)
                                                                                        cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCgg                                                          >  5:371752/1‑148 (MQ=255)
                                                                                        cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCgg                                                          >  2:301387/1‑148 (MQ=255)
                                                                                        cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGc                                                         <  1:301387/149‑1 (MQ=255)
                                                                                               ttttttGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACaa                                                  >  1:278246/1‑149 (MQ=255)
                                                                                               accgttccaacactgaaagaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCt                                                                           >  1:385432‑M1/42‑124 (MQ=255)
                                                                                               accgttccaacactgaaagaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCt                                                                           <  2:385432‑M1/83‑1 (MQ=255)
                                                                                                 ttttGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAAt                                                 <  4:281879/148‑1 (MQ=255)
                                                                                                    tGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                              >  8:198047/1‑148 (MQ=255)
                                                                                                     gCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCCGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTcc                                             <  1:215822/148‑1 (MQ=255)
                                                                                                      cTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCa                                            <  6:371752/148‑1 (MQ=255)
                                                                                                       aacactgaaagaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                              <  7:35729‑M1/112‑1 (MQ=255)
                                                                                                         aCCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAg                                                                                         <  4:343455/100‑1 (MQ=255)
                                                                                                         aCCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTaa                                                              <  4:125716/127‑1 (MQ=255)
                                                                                                         aCCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTaa                                                              >  3:125716/1‑127 (MQ=255)
                                                                                                           cAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCt                                      >  6:39869/1‑149 (MQ=255)
                                                                                                            gatgtgaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGGTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTg                                     >  3:253420‑M1/29‑149 (MQ=255)
                                                                                                             gACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTgg                                    <  7:198047/149‑1 (MQ=255)
                                                                                                                 tttGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGcac                                                       <  7:92801/126‑1 (MQ=255)
                                                                                                                 tttGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGcac                                                       >  8:92801/1‑126 (MQ=255)
                                                                                                                  aaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTg                                     <  4:253420‑M1/121‑1 (MQ=255)
                                                                                                                   tGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                              >  3:191022/1‑133 (MQ=255)
                                                                                                                   tGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                              <  4:191022/133‑1 (MQ=255)
                                                                                                                     ccTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACaa                             <  5:39869/148‑1 (MQ=255)
                                                                                                                      cattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCCGGCCGACAata                           >  8:35729‑M1/19‑149 (MQ=255)
                                                                                                                       tttAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTccc                                                                       <  8:253530/104‑1 (MQ=255)
                                                                                                                       tttAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTccc                                                                       >  7:253530/1‑104 (MQ=255)
                                                                                                                         tAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATc                         <  7:249032/148‑1 (MQ=255)
                                                                                                                           aCGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCaaa                      >  6:121358/1‑149 (MQ=255)
                                                                                                                           aCGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCaaa                      <  4:339070/149‑1 (MQ=255)
                                                                                                                            cgcgCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAAc                     <  2:148557/149‑1 (MQ=255)
                                                                                                                               gcAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGc                  >  1:9233/1‑149 (MQ=255)
                                                                                                                                   tCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAAtt                                                >  8:213396/1‑115 (MQ=255)
                                                                                                                                   tCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAAtt                                                <  7:213396/115‑1 (MQ=255)
                                                                                                                                     gTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCa                                            <  5:344890/117‑1 (MQ=255)
                                                                                                                                     gTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCa                                            >  6:344890/1‑117 (MQ=255)
                                                                                                                                        GCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTgg          >  2:278246‑M1/1‑148 (MQ=255)
                                                                                                                                        GCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGAACCCAGCGCCGCTTCCCACGGGCTAACCGTCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATTTGgc         <  2:165864‑M1/149‑1 (MQ=255)
                                                                                                                                         CCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGgcg        >  8:261453‑M1/1‑149 (MQ=255)
                                                                                                                                          CCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGc                                                         >  7:35624‑M1/1‑99 (MQ=255)
                                                                                                                                          CCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGc                                                         <  8:35624‑M1/99‑1 (MQ=255)
                                                                                                                                              ccTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCt                                                                <  5:255340/88‑1 (MQ=255)
                                                                                                                                              ccTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCt                                                                >  6:255340/1‑88 (MQ=255)
                                                                                                                                               cTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAatat  >  5:113875/1‑149 (MQ=255)
                                                                                                                                                tGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCCCCCAGCGCCGCTTCCCCCGGGCTAACCGGCACCACAATTTCAAGATCCTGGCCGACAATATCCAACCGCGGATGTGGCGCAatat  >  3:83300/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGGCGTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATAT  >  NC_000913/1062895‑1063186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑