Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,063,047 T→G 5.9% P243P (CCA→CCC cbpA ← DnaK co‑chaperone; curved DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,063,0470TG 5.9% 304.7 / 7.6 101P243P (CCA→CCCcbpADnaK co‑chaperone; curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (41/54);  new base G (3/3);  total (44/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 102 total aligned reads displayed.

GTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACC  >  NC_000913/1062900‑1063194
                                                                                                                                                   |                                                                                                                                                   
gTCTGCCCGTTGCTGCCTGAGCGCGGCAGTGTTTTCTTCGGGGTTCGGCGGCATGACGATTTTGAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCTGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTg                                                                                                                                                    <  3:254319/149‑1 (MQ=255)
    gCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCgg                                                                                                                                                >  8:229794/1‑149 (MQ=255)
     ccAGTTGCTGCCACAGCGCGGCTGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCGTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCggg                                                                                                                                               >  2:215822/1‑149 (MQ=255)
     ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGc                                                                                                                                                  <  3:227486/146‑1 (MQ=255)
     ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCggg                                                                                                                                               <  2:361649/149‑1 (MQ=255)
     ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCgg                                                                                                                                                >  1:15228/1‑148 (MQ=255)
     ccAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGGCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCgg                                                                                                                                                <  5:237802/148‑1 (MQ=255)
      cAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGa                                                                                                                                              <  4:121570/149‑1 (MQ=255)
            ctgcCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGt                                                                                                                                          >  4:177635/1‑147 (MQ=255)
             tgcCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAg                                                                                                                                       >  3:339070/1‑149 (MQ=255)
               ccACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAg                                                                                                                                       <  7:104396/147‑1 (MQ=255)
               ccACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGc                                                                                                                                      >  8:44826/1‑148 (MQ=255)
               ccACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGc                                                                                                                                      >  2:408440/1‑148 (MQ=255)
                cacaGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCa                                                                                                                                     >  1:401936/1‑148 (MQ=255)
                  caGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCaaaa                                                                                                                                  <  7:44826/149‑1 (MQ=255)
                     cgcgGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGc                                                                                                                               <  4:348248/149‑1 (MQ=255)
                          cAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttct                                                                                                                          <  5:243771/149‑1 (MQ=255)
                           aGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttctt                                                                                                                         <  2:356975/149‑1 (MQ=255)
                           aGTGGTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCGGCCTGGCGGGATAGTCAGCAAAATGctttctt                                                                                                                         <  1:325696/149‑1 (MQ=255)
                            gtgtTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGctttcttt                                                                                                                        <  3:177635/149‑1 (MQ=255)
                               ttttCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCa                                                                                                                      <  2:286488/148‑1 (MQ=255)
                                    aTCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTg                                                                                                                <  7:229794/149‑1 (MQ=255)
                                    aTCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTg                                                                                                                <  4:176178/149‑1 (MQ=255)
                                       cGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGaa                                                                                                             >  1:376967/1‑149 (MQ=255)
                                            tCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                         <  5:33757/148‑1 (MQ=255)
                                            tCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTAAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                         <  1:7639/148‑1 (MQ=255)
                                                  gcATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTa                                                                                                  <  2:9003/149‑1 (MQ=255)
                                                     tCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATGGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTa                                                                                                  <  7:20331/146‑1 (MQ=255)
                                                     tCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATGGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTa                                                                                                  >  8:20331/1‑146 (MQ=255)
                                                     tCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCa                                                                                               >  1:191467/1‑149 (MQ=255)
                                                       aCGATTTTCAGTTCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCACAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                         <  2:85748/137‑1 (MQ=255)
                                                       aCGATTTTCAGTGCCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCACAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                         >  1:85748/1‑137 (MQ=255)
                                                       aCGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACttt                                                                                                   >  5:214194/1‑143 (MQ=255)
                                                       aCGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACttt                                                                                                   <  6:214194/143‑1 (MQ=255)
                                                           ttttCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                         <  5:231826/133‑1 (MQ=255)
                                                           ttttCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGt                                                                                                         >  6:231826/1‑133 (MQ=255)
                                                              tCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAgcgc                                                                                       <  1:408440/148‑1 (MQ=255)
                                                                aGTACCGCATACAGATCGCCGGGCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGcgccg                                                                                     <  5:340433/148‑1 (MQ=255)
                                                                 gTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCt                                                                                   >  1:148557/1‑149 (MQ=255)
                                                                    ccGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTcc                                                                                >  3:281879/1‑149 (MQ=255)
                                                                      gCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCa                                                                              <  2:191467/149‑1 (MQ=255)
                                                                       cATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                                    >  8:235267/1‑110 (MQ=255)
                                                                       cATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                                    <  7:235267/110‑1 (MQ=255)
                                                                         tACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACgg                                                                           <  2:376967/149‑1 (MQ=255)
                                                                          aCAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACgg                                                                           <  4:134051/148‑1 (MQ=255)
                                                                           cAGTTCGCCGGTCTTTATTATGCTCACCAGACCTTTGCCTTTAACGCGCAATCATTGCCCGGCCTGGCTGCCTGGCGGGATTGTCAGCAATATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACggg                                                                          <  2:352956/148‑1 (MQ=255)
                                                                           cAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACggg                                                                          <  2:401936/148‑1 (MQ=255)
                                                                            aGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCt                                                                        <  5:379643/149‑1 (MQ=255)
                                                                              aTCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACg                                                                            >  6:201084/1‑143 (MQ=255)
                                                                              aTCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACg                                                                            <  5:201084/143‑1 (MQ=255)
                                                                              aTCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTaa                                                                      >  7:261453/1‑149 (MQ=255)
                                                                                   cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                                    >  1:383635/1‑98 (MQ=255)
                                                                                   cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAgt                                                                                                                    <  2:383635/98‑1 (MQ=255)
                                                                                   cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCgg                                                                  >  5:371752/1‑148 (MQ=255)
                                                                                   cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCgg                                                                  >  2:301387/1‑148 (MQ=255)
                                                                                   cGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGc                                                                 <  1:301387/149‑1 (MQ=255)
                                                                                          ttttttGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACaa                                                          >  1:278246/1‑149 (MQ=255)
                                                                                          accgttccaacactgaaagaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCt                                                                                   >  1:385432‑M1/42‑124 (MQ=255)
                                                                                          accgttccaacactgaaagaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCt                                                                                   <  2:385432‑M1/83‑1 (MQ=255)
                                                                                            ttttGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAAt                                                         <  4:281879/148‑1 (MQ=255)
                                                                                               tGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                                      >  8:198047/1‑148 (MQ=255)
                                                                                                gCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCCGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTcc                                                     <  1:215822/148‑1 (MQ=255)
                                                                                                 cTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCa                                                    <  6:371752/148‑1 (MQ=255)
                                                                                                  aacactgaaagaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                                      <  7:35729‑M1/112‑1 (MQ=255)
                                                                                                    aCCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAg                                                                                                 <  4:343455/100‑1 (MQ=255)
                                                                                                    aCCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTaa                                                                      >  3:125716/1‑127 (MQ=255)
                                                                                                    aCCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTaa                                                                      <  4:125716/127‑1 (MQ=255)
                                                                                                      cAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCt                                              >  6:39869/1‑149 (MQ=255)
                                                                                                       gatgtgaaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGGTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTg                                             >  3:253420‑M1/29‑149 (MQ=255)
                                                                                                        gACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTgg                                            <  7:198047/149‑1 (MQ=255)
                                                                                                            tttGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGcac                                                               <  7:92801/126‑1 (MQ=255)
                                                                                                             aaagcattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTg                                             <  4:253420‑M1/121‑1 (MQ=255)
                                                                                                              tGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                                      >  3:191022/1‑133 (MQ=255)
                                                                                                              tGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTc                                                      <  4:191022/133‑1 (MQ=255)
                                                                                                                ccTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACaa                                     <  5:39869/148‑1 (MQ=255)
                                                                                                                 cattttgctgactatcccGCCAGGCAGCCAGGCCGGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCCGGCCGACAata                                   >  8:35729‑M1/19‑149 (MQ=255)
                                                                                                                  tttAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTccc                                                                               >  7:253530/1‑104 (MQ=255)
                                                                                                                  tttAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTccc                                                                               <  8:253530/104‑1 (MQ=255)
                                                                                                                    tAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATc                                 <  7:249032/148‑1 (MQ=255)
                                                                                                                      aCGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCaaa                              >  6:121358/1‑149 (MQ=255)
                                                                                                                       cgcgCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAAc                             <  2:148557/149‑1 (MQ=255)
                                                                                                                          gcAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGc                          >  1:9233/1‑149 (MQ=255)
                                                                                                                              tCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAAtt                                                        <  7:213396/115‑1 (MQ=255)
                                                                                                                              tCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAAtt                                                        >  8:213396/1‑115 (MQ=255)
                                                                                                                                gTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCa                                                    >  6:344890/1‑117 (MQ=255)
                                                                                                                                gTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCa                                                    <  5:344890/117‑1 (MQ=255)
                                                                                                                                   GCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTgg                  >  2:278246‑M1/1‑148 (MQ=255)
                                                                                                                                   GCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGAACCCAGCGCCGCTTCCCACGGGCTAACCGTCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATTTGgc                 <  2:165864‑M1/149‑1 (MQ=255)
                                                                                                                                    CCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGgcg                >  8:261453‑M1/1‑149 (MQ=255)
                                                                                                                                     CCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGc                                                                 >  7:35624‑M1/1‑99 (MQ=255)
                                                                                                                                     CCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGc                                                                 <  8:35624‑M1/99‑1 (MQ=255)
                                                                                                                                         ccTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCt                                                                        >  6:255340/1‑88 (MQ=255)
                                                                                                                                         ccTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCt                                                                        <  5:255340/88‑1 (MQ=255)
                                                                                                                                          cTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAatat          >  5:113875/1‑149 (MQ=255)
                                                                                                                                           tGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCCCCCAGCGCCGCTTCCCCCGGGCTAACCGGCACCACAATTTCAAGATCCTGGCCGACAATATCCAACCGCGGATGTGGCGCAatat          >  3:83300/1‑148 (MQ=255)
                                                                                                                                                gCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCTGACAATATCAAACAGCGGATGTGGCGCAata           <  5:125010/142‑1 (MQ=255)
                                                                                                                                                gCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCGGACAATATCAAACAGCGGATGTGGCGCAata           >  6:125010/1‑142 (MQ=255)
                                                                                                                                                gCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCa    >  6:333099/1‑149 (MQ=255)
                                                                                                                                                  cTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCAcc  <  2:9233/149‑1 (MQ=255)
                                                                                                                                                   tGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGACTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCAc   >  5:358419/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCTGCCAGTTGCTGCCACAGCGCGGCAGTGTTTTCATCCGGTTTCGGCGGCATCACGATTTTCAGTACCGCATACAGATCGCCGGTCTGTTTTTTGCTCACCAGACCTTTGCCTTTAACGCGCAATCGTTGCCCGGCCTGGCTGCCTGGCGGGATAGTCAGCAAAATGCTTTCTTTCAGTGTTGGAACGGTGACTTTAGCACCCAGCGCCGCTTCCCACGGGCTAACCGGCACCACAATTTCCAGATCCTGGCCGACAATATCAAACAGCGGATGTGGCGCAATATGAATCACC  >  NC_000913/1062900‑1063194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: