Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,980,242 T→G 6.3% R457R (CGA→CGC otsA ← trehalose‑6‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,980,2420TG 6.3% 329.0 / 12.2 107R457R (CGA→CGCotsAtrehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (48/52);  new base G (5/2);  total (53/54)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 109 total aligned reads displayed.

TTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTGC  >  NC_000913/1980094‑1980385
                                                                                                                                                    |                                                                                                                                               
tttCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCtt                                                                                                                                                 >  1:281622/1‑149 (MQ=255)
  tCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTc                                                                                                                                                <  7:138997/148‑1 (MQ=255)
   cTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTcg                                                                                                                                               <  7:89084/148‑1 (MQ=255)
        cgcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGaa                                                                                                                                          <  5:246977/148‑1 (MQ=255)
        cgcgAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAAc                                                                                                                                         >  3:311227/1‑149 (MQ=255)
             gCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCt                                                                                                                                    >  5:318387/1‑149 (MQ=255)
              cATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCt                                                                                                                                    <  2:129151/148‑1 (MQ=255)
                tCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGATATTAACAGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCt                                                                                                                                 >  1:100650/1‑149 (MQ=255)
                     cTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                                                                                             <  8:415394/148‑1 (MQ=255)
                        ggCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCg                                                                                                                         <  4:311227/149‑1 (MQ=255)
                         gCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCtt                                                                                                                                >  7:125001/1‑141 (MQ=255)
                         gCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCtt                                                                                                                                <  8:125001/141‑1 (MQ=255)
                         gCGAAAAGAGTTATCGCCTCACGGGAGGTCGATGTGCTGCTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGc                                                                                                                        <  2:281622/149‑1 (MQ=255)
                             aaaGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAAt                                                                                                                    >  3:362538/1‑149 (MQ=255)
                             aaaGAGTTATTGCCTCAAGTGAGGACGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGATTTCCGCGCTTCGCGCAACTATCTGCTTTAGGTCGCTAAt                                                                                                                    >  6:119542/1‑149 (MQ=255)
                               agagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTATTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGa                                                                                                                  <  2:190548/149‑1 (MQ=255)
                                gagTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGaa                                                                                                                 <  6:318387/149‑1 (MQ=255)
                                      ttGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           <  5:169992/149‑1 (MQ=255)
                                      ttGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           >  7:339937/1‑149 (MQ=255)
                                         ccTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTg                                                                                                        >  6:380259/1‑149 (MQ=255)
                                          cTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                                                                                             <  7:124882/127‑1 (MQ=255)
                                          cTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAg                                                                                                                             >  8:124882/1‑127 (MQ=255)
                                           tCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGc                                                                                                       >  7:226401/1‑148 (MQ=255)
                                              aGTGAGGACGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGt                                                                                                   <  8:5820/149‑1 (MQ=255)
                                               gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTg                                                                                                  <  8:189413/149‑1 (MQ=255)
                                               gTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCGCCTACGCACGCTTTGGAGAGGTAGCAACTTGATCGCGCTGCTGGCTTTCCGCGCGTCGCGGAACGATCTGCTTTAGGTCGCTAAGGAAGCAGGCGGGCCAGTg                                                                                                  >  4:55764/1‑149 (MQ=255)
                                                  aGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGtt                                                                                               <  1:28783/149‑1 (MQ=255)
                                                     tCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAat                                                                                            <  8:226401/149‑1 (MQ=255)
                                                           tgCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGtt                                                                                      <  5:278861/149‑1 (MQ=255)
                                                            gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGc                                                                                                                                  <  3:160344/104‑1 (MQ=255)
                                                            gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGc                                                                                                                                  >  4:160344/1‑104 (MQ=255)
                                                            gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           >  5:106457/1‑127 (MQ=255)
                                                            gCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGGAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTc                                                                                                           <  6:106457/127‑1 (MQ=255)
                                                              tGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTATTGAAGCAATCCTGCCAGTGGTTAATATCGttttt                                                                                   >  8:331183/1‑149 (MQ=255)
                                                               gTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTATTTc                                                                                  >  2:308177/1‑149 (MQ=255)
                                                                ttAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGttttt                                                                                   <  3:55764/147‑1 (MQ=255)
                                                                ttAGTGCCACGGACGGGAGATTAGGGGCTCCTACGCCAGCTTTGGAACGGTAGCAACTTTAGCGCGGTGCTGGCTTTCCGCGCTTCGGGGTTCTCTCAGCGTTAGGTCGCGATTGAAGCAGTCCTGCGAGGGGTTAATATCGGTTTTcc                                                                                 >  8:192970/1‑148 (MQ=255)
                                                                    ttCCACTTACGGGAGATTAACCACTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTATGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGa                                                                              >  5:56233/1‑148 (MQ=255)
                                                                     tCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                                                                                              >  3:363543/1‑115 (MQ=255)
                                                                     tCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCa                                                                                                              <  4:363543/115‑1 (MQ=255)
                                                                       cACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCtt                                                                                                                                >  5:273086/1‑95 (MQ=255)
                                                                       cACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAAc                                                                          <  2:331315/149‑1 (MQ=255)
                                                                         cTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGt                                                                        <  1:8444/149‑1 (MQ=255)
                                                                          ttACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTc                                                                       >  3:130736/1‑149 (MQ=255)
                                                                              gggAGATTAACCGCTCCTCCGCAAGCTTTGGAAAGGTCGCAACTTTATCGCGCTGCTGGCTGTCCGCGCTTCGCGGAACTATCTGCGTTAGGTCGCTAATGAAGCACTCCTGCCAGGGGGTAATATCGTTTTTCACGATAACGTCCa                                                                     <  3:62595/147‑1 (MQ=255)
                                                                              gggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCACCTTTATCGCGCTGCTGGCTTTCCGCGCTGCGCGGAACTATCTGCTTTAGGTCGCTAATGCAGCACTCCTGCCAGGGGTTAATATCGTTTTTCACGATACCGTCCa                                                                     >  4:62595/1‑147 (MQ=255)
                                                                              gggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAg                                                                    >  7:313613/1‑148 (MQ=255)
                                                                               ggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGcc                                                                                                      >  1:61851/1‑113 (MQ=255)
                                                                               ggAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGcc                                                                                                      <  2:61851/113‑1 (MQ=255)
                                                                                    ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCg                                                                                        >  2:303628/1‑122 (MQ=255)
                                                                                    ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCg                                                                                        <  1:303628/122‑1 (MQ=255)
                                                                                    ttAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                             <  5:143783/149‑1 (MQ=255)
                                                                                     tAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTg                                                            >  7:417/1‑149 (MQ=255)
                                                                                      aaCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTg                                                            <  5:380259/148‑1 (MQ=255)
                                                                                       aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                             >  6:215279/1‑146 (MQ=255)
                                                                                       aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCt                                                             <  5:215279/146‑1 (MQ=255)
                                                                                       aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                          <  6:246543/149‑1 (MQ=255)
                                                                                       aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                          >  5:246543/1‑149 (MQ=255)
                                                                                       aCCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCa                                                          <  3:34828/149‑1 (MQ=255)
                                                                                        ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCAt                                                         >  8:1248/1‑149 (MQ=255)
                                                                                        ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        <  8:270881/70‑3 (MQ=255)
                                                                                        ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        >  7:270881/1‑68 (MQ=255)
                                                                                        ccGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGAAGCGCGGaaag                                                                                                                                        >  7:277319/1‑68 (MQ=255)
                                                                                          gCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGa                                                       <  8:48293/149‑1 (MQ=255)
                                                                                           cTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGAc                                                      >  8:269382/1‑149 (MQ=255)
                                                                                              cTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTTGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACgg                                                    >  6:120331/1‑148 (MQ=255)
                                                                                              atACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGAGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAATCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACggg                                                   <  7:331183/148‑1 (MQ=255)
                                                                                                  gCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGaaa                                                >  4:1553/1‑148 (MQ=255)
                                                                                                    aaGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATAc                                             >  8:331417/1‑149 (MQ=255)
                                                                                                    aaGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATAc                                             >  3:276648/1‑149 (MQ=255)
                                                                                                     aGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAAc                                                                          <  4:286549/119‑1 (MQ=255)
                                                                                                     aGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAAc                                                                          >  3:286549/1‑119 (MQ=255)
                                                                                                      gCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGt                                           <  3:1553/149‑1 (MQ=255)
                                                                                                       cTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGtt                                          >  7:292249/1‑149 (MQ=255)
                                                                                                          tGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAg                                                                    >  8:282685/1‑120 (MQ=255)
                                                                                                          tGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAg                                                                    <  7:282685/120‑1 (MQ=255)
                                                                                                          tGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTcc                                        <  8:417/148‑1 (MQ=255)
                                                                                                             aaaGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGc                                      >  2:311347/1‑147 (MQ=255)
                                                                                                                    gCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACTGGAAATACTTTCCGCCAGCGACa                              >  2:122625/1‑148 (MQ=255)
                                                                                                                     cAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATa                            <  1:311347/149‑1 (MQ=255)
                                                                                                                     cAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATa                            <  8:339937/149‑1 (MQ=255)
                                                                                                                       aCTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCAGCCAGCGACa                              >  5:89130/1‑145 (MQ=255)
                                                                                                                       aCTTTATCGCGCTGCTGGCTTTCCGCGCTTAGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGt                          <  1:78466/149‑1 (MQ=255)
                                                                                                                       aCTTTATCGCGCTGATTTATTTCCGCGCTTCGCGGAACTATCTGCTTTAGTTCTCTTATGAAGAACTCCTGTCAGTGGTTAATATCTTTTTTCTCTATATGTTGCAGCATTTCTGCATGACGGGAATTACGTTCAGcctgataaa                              <  6:89130/145‑8 (MQ=255)
                                                                                                                           tATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCaa                       <  8:313613/148‑1 (MQ=255)
                                                                                                                           tATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCa                        <  1:234059/147‑1 (MQ=255)
                                                                                                                              cgcgCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGt                                           <  2:376663/125‑1 (MQ=255)
                                                                                                                              cgcgCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGt                                           >  1:376663/1‑125 (MQ=255)
                                                                                                                              cgcgCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCa                   >  7:77603/1‑149 (MQ=255)
                                                                                                                                   tgctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATc              >  7:297402/1‑149 (MQ=255)
                                                                                                                                     ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCaa                       >  8:180585/1‑138 (MQ=255)
                                                                                                                                     ctgGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCaa                       <  7:180585/138‑1 (MQ=255)
                                                                                                                                       ggCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgc          >  7:157582/1‑149 (MQ=255)
                                                                                                                                       atagttCCGCGAAGCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgc          >  1:113700/5‑149 (MQ=255)
                                                                                                                                        gCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgcg         >  8:46273/1‑149 (MQ=255)
                                                                                                                                        gCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAAAACGTTCCGCCAGCGACATAGTCAATGCACGATCCAgcg         >  7:407764/1‑149 (MQ=255)
                                                                                                                                           ttCCGCGAAGCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAg      >  8:325307/1‑149 (MQ=255)
                                                                                                                                              cgcgCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCt    >  1:400347/1‑148 (MQ=255)
                                                                                                                                              cgcgCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCt    <  2:400347/148‑1 (MQ=255)
                                                                                                                                               gcgcTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTGc  <  7:46273/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TTTCTTTTCGCGAGCATCCTGCTCGGCGAAAAGAGTTATTGCCTCAAGTGAGGTCGATGTGCTGTTAGTTCCACTTACGGGAGATTAACCGCTCCTACGCAAGCTTTGGAAAGGTAGCAACTTTATCGCGCTGCTGGCTTTCCGCGCTTCGCGGAACTATCTGCTTTAGGTCGCTAATGAAGCACTCCTGCCAGTGGTTAATATCGTTTTTCACGATAACGTCCAGCATTTCTGCATGACGGGAAATACGTTCCGCCAGCGACATAGTCAATGCACGATCCAGCGCAGCTGC  >  NC_000913/1980094‑1980385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: