Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,699,290 T→C 5.1% S178P (TCC→CCC)  yfhH → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,699,2900TC 5.1% 310.0 / 5.0 99S178P (TCC→CCC) yfhHputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (39/54);  new base C (2/3);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01

Only 100 of 102 total aligned reads displayed.

CGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTG  >  NC_000913/2699146‑2699438
                                                                                                                                                |                                                                                                                                                    
cgcTCTGCGCGGCGGATAATTCTGACCGGTATTGTCTCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATTAAGATTGTCTTCAATGCTGCCGCAGTTCGATATATGCATGCGCTACTCGCAACAGTACAGGCGTCTTccc                                                                                                                                                   <  2:161768/148‑1 (MQ=255)
cgcTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTccc                                                                                                                                                   <  4:229457/148‑1 (MQ=255)
   tctGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGa                                                                                                                                               >  1:295602/1‑149 (MQ=255)
      gcgcGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGa                                                                                                                                            >  3:204860/1‑149 (MQ=255)
       cgcgGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGAc                                                                                                                                              <  3:375438/146‑1 (MQ=255)
       cgcgGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGa                                                                                                                                            <  1:146497/148‑1 (MQ=255)
       cgcgGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGAt                                                                                                                                           <  1:93801/149‑1 (MQ=255)
       cgcgGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGATTCAATGCCGCCGCAGTGCGCTATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGa                                                                                                                                            <  1:12444/148‑1 (MQ=255)
            cggATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGtt                                                                                                                                      <  4:177815/149‑1 (MQ=255)
            cggATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGtt                                                                                                                                      <  1:114196/149‑1 (MQ=255)
            cggATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGtt                                                                                                                                      <  2:219133/149‑1 (MQ=255)
             ggATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGtta                                                                                                                                     >  5:66550/1‑149 (MQ=255)
             ggAGAATTCTGCCCGGTATTGGCGCTTCGGGTCTGCTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTAAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGGCCCCTGACGATCTGtta                                                                                                                                     >  5:66548/1‑149 (MQ=255)
              gATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGttat                                                                                                                                    <  3:298904/149‑1 (MQ=255)
               aTAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGttatt                                                                                                                                   <  1:394123/149‑1 (MQ=255)
                tAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGttatta                                                                                                                                  <  8:231491/149‑1 (MQ=255)
                  aTTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAg                                                                                                                                 <  3:296426/148‑1 (MQ=255)
                   ttCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCTTCCCCTGACGATCTGTTATTAGc                                                                                                                                >  3:57644/1‑148 (MQ=255)
                   ttCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGc                                                                                                                                >  4:82351/1‑148 (MQ=255)
                    tCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCa                                                                                                                              >  2:185188/1‑149 (MQ=255)
                     cTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCa                                                                                                                              <  5:331196/148‑1 (MQ=255)
                       gACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCAttt                                                                                                                           >  1:266919/1‑149 (MQ=255)
                        aCCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCAtt                                                                                                                            <  4:204860/147‑1 (MQ=255)
                        aCCGGTATTCGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCAt                                                                                                                             <  4:305351/146‑1 (MQ=255)
                            gTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTa                                                                                                                       >  6:96327/1‑148 (MQ=255)
                                  gcgcTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCgtt                                                                                                                >  6:257972/1‑147 (MQ=255)
                                  gcgcTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGgt                                                                                                                >  6:181185/1‑149 (MQ=255)
                                           gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGAcgc                                                                                                       >  4:263786/1‑149 (MQ=255)
                                           gTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGAcgc                                                                                                       >  8:105280/1‑149 (MQ=255)
                                                gtggCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAg                                                                                                    <  5:96327/147‑1 (MQ=255)
                                                gtggCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGt                                                                                                   >  4:248618/1‑148 (MQ=255)
                                                   gcgcAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTaa                                                                                                <  3:197390/148‑1 (MQ=255)
                                                    cgcAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAAc                                                                                              <  6:79932/149‑1 (MQ=255)
                                                     gcAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAAcc                                                                                             >  3:279320/1‑149 (MQ=255)
                                                     gcAAAACTTTGCCTGGAAGCTGATGAAGATTGGCATCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAAcc                                                                                             >  1:342925/1‑149 (MQ=255)
                                                      cAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGgt                                                                                                                <  6:7528/129‑1 (MQ=255)
                                                      cAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGgt                                                                                                                >  5:7528/1‑129 (MQ=255)
                                                      cAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCt                                                                                            >  5:177921/1‑149 (MQ=255)
                                                      cAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCt                                                                                            <  5:289637/149‑1 (MQ=255)
                                                        aaaCTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTCCAGGCGTCGTACCCTGACGAGCTGTTATTAGCCCTTTCCTACACCGGTGTACGACGCGAGTTAAACCTgg                                                                                          <  4:141241/149‑1 (MQ=255)
                                                          aCTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTggcg                                                                                        <  2:266919/149‑1 (MQ=255)
                                                          aCTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTggcg                                                                                        <  6:177921/149‑1 (MQ=255)
                                                            tttGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACCGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTggcggc                                                                                      >  7:285461/1‑149 (MQ=255)
                                                                ccTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  <  3:263786/149‑1 (MQ=255)
                                                                ccTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCaga                                                                                   <  1:185188/148‑1 (MQ=255)
                                                                 cTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagatg                                                                                 <  7:105280/149‑1 (MQ=255)
                                                                 cTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  >  3:194584/1‑148 (MQ=255)
                                                                 cTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGCCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  >  8:293112/1‑148 (MQ=255)
                                                                 cTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGCCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  >  8:288119/1‑148 (MQ=255)
                                                                   ggAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  >  8:65374/1‑146 (MQ=255)
                                                                   ggAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  <  2:333429/146‑1 (MQ=255)
                                                                   ggAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCagat                                                                                  >  1:333429/1‑146 (MQ=255)
                                                                    gtaGCGGATGAATATTGGCTTCAATGCTGCCGCTGTGCGCGATATGCATGCGCTACTGGCAACAGTACAGGAGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGAGGCagatgaga                                                                              <  5:108743/147‑1 (MQ=255)
                                                                        cTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGAtgct                                                                          <  3:82351/149‑1 (MQ=255)
                                                                           atgaAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGCCGCGAGTTAAACCTGGCGGCAGATGAGAtgctgc                                                                        >  6:350742/1‑148 (MQ=255)
                                                                            tgaAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGCCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGa                                                                      <  7:288119/149‑1 (MQ=255)
                                                                            tgaAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGCCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGa                                                                      <  7:293112/149‑1 (MQ=255)
                                                                            agaAGATTGGCTTAAATGCTGCCGCAGTGCGCGATCTGCATGCGCTACTCGCAACAGTACAGGCGTCGCCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGa                                                                      <  7:288125/148‑1 (MQ=255)
                                                                                 aTTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGa                                                                                                     >  8:371140/1‑113 (MQ=255)
                                                                                 aTTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGa                                                                                                     <  7:371140/113‑1 (MQ=255)
                                                                                  ttGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGAGCTGTTATTAGCCATTTCCTACACCGCTGTACGACGCGAGTTAACACTGGCGGCAGATGAGATGCTGCGAGTGGGc                                                                >  1:194806/1‑149 (MQ=255)
                                                                                    ggCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCATATGAGATGCTGCGAGTGGGCg                                                               <  3:163428/148‑1 (MQ=255)
                                                                                     gCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCgg                                                              <  5:257972/148‑1 (MQ=255)
                                                                                     gCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCgg                                                              >  5:219512/1‑148 (MQ=255)
                                                                                      cTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGCCGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGa                                                             >  2:88017/1‑148 (MQ=255)
                                                                                      cTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGaa                                                            <  4:194584/149‑1 (MQ=255)
                                                                                       ttCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGAtgctgc                                                                        <  8:276944/136‑1 (MQ=255)
                                                                                       ttCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGAtgctgc                                                                        >  7:276944/1‑136 (MQ=255)
                                                                                         cAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGaaaa                                                          <  4:279320/148‑1 (MQ=255)
                                                                                         cAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGaaaa                                                          <  8:146325/148‑1 (MQ=255)
                                                                                         cAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGAGCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAg                                                         >  3:71689/1‑149 (MQ=255)
                                                                                            tgctgcCGCAGTGCGCGATTTGCTTGCGCTAGTCGCAACAGTACTGGCGTCGTCCCCTGACGATCTGTTATTAGCGATTTCGTACTCCGGTGTACTAGGCTAGTTAAAGCTGGAGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGc                                                      <  4:71689/149‑1 (MQ=255)
                                                                                              ctgcCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTg                                                    <  5:109952/149‑1 (MQ=255)
                                                                                                 ccGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGc                                                  <  5:181185/148‑1 (MQ=255)
                                                                                                         cgcgATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAAcc                                                                                             >  1:252075/1‑97 (MQ=255)
                                                                                                         cgcgATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAAcc                                                                                             <  2:252075/97‑1 (MQ=255)
                                                                                                         cgcgATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCg                                          <  8:118815/148‑1 (MQ=255)
                                                                                                            gATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGAGCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCtt                                      <  3:248618/149‑1 (MQ=255)
                                                                                                                tgcatgcGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCCGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGAtgctgc                                                                        <  4:70811/111‑1 (MQ=255)
                                                                                                                tgcatgcGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCCGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGAtgctgc                                                                        >  3:70811/1‑111 (MQ=255)
                                                                                                                  catgcGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTcc                                <  2:295602/149‑1 (MQ=255)
                                                                                                                          aCTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGccc                        >  1:298516/1‑149 (MQ=255)
                                                                                                                           cTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGaa                             <  7:114430/143‑1 (MQ=255)
                                                                                                                           cTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGaa                             >  8:114430/1‑143 (MQ=255)
                                                                                                                              gCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTTCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGc                     <  2:342925/148‑1 (MQ=255)
                                                                                                                              gCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGa                                                                      <  5:213153/99‑1 (MQ=255)
                                                                                                                              gCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGa                                                                      >  6:213153/1‑99 (MQ=255)
                                                                                                                              gCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAAt                            <  1:208281/141‑1 (MQ=255)
                                                                                                                              gCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAAt                            >  2:208281/1‑141 (MQ=255)
                                                                                                                              gCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGc                     >  4:65083/1‑148 (MQ=255)
                                                                                                                                aaCAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCaa                   >  5:323499/1‑148 (MQ=255)
                                                                                                                                  cAGTTCAGGCGTCGTCCCCTTAAGATCTGTTATTAGCCATTTCCTACTCCGGTGTGAGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAg                <  7:354064/149‑1 (MQ=255)
                                                                                                                                  cAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCaa                   >  8:183120/1‑146 (MQ=255)
                                                                                                                                  cAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCaa                   <  7:183120/146‑1 (MQ=255)
                                                                                                                                     tACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGCTGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGt             >  2:43219/1‑149 (MQ=255)
                                                                                                                                        aGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCt          >  4:98918/1‑149 (MQ=255)
                                                                                                                                         ggCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCtt         <  3:98918/149‑1 (MQ=255)
                                                                                                                                          gCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGaaaa                                                          <  6:87419/99‑1 (MQ=255)
                                                                                                                                               gtcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCAtt   <  3:65083/149‑1 (MQ=255)
                                                                                                                                                tcCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTg  >  6:162329/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CGCTCTGCGCGGCGGATAATTCTGACCGGTATTGGCGCTTCGGGTCTGGTGGCGCAAAACTTTGCCTGGAAGCTGATGAAGATTGGCTTCAATGCTGCCGCAGTGCGCGATATGCATGCGCTACTCGCAACAGTACAGGCGTCGTCCCCTGACGATCTGTTATTAGCCATTTCCTACACCGGTGTACGACGCGAGTTAAACCTGGCGGCAGATGAGATGCTGCGAGTGGGCGGAAAAGTGCTGGCGATTACCGGCTTTACTCCGAATGCCCTGCAACAGCGTGCTTCTCATTG  >  NC_000913/2699146‑2699438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: