Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,789,148 A→T 5.5% Q55L (CAG→CTG)  csiD → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,789,1480AT 5.5% 282.9 / 3.3 88Q55L (CAG→CTG) csiDcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (49/34);  new base T (3/2);  total (52/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCGTACAAAATAACGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGAT  >  NC_000913/2789000‑2789296
                                                                                                                                                    |                                                                                                                                                    
gccgTACAAAATAACGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCa                                                                                                                                                      <  7:260738/149‑1 (MQ=255)
    tACAAAATAACGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCTGgcg                                                                                                                                                  >  4:162895/1‑149 (MQ=255)
     aCAAAATAACGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGgcgc                                                                                                                                                 >  8:66128/1‑149 (MQ=255)
      cAAAATAACGCTGTCGATACAGGCCAGGACTATATCGGATTCACACTCACCCCGTCGGCGCAATCCCCGCGACTGCAGGAACTCACCTTCAACGAACAGACGACCAAACAGATTCTTCAGCAGGATGCCGAGTGGCCCGAGCAGGCGCt                                                                                                                                                >  4:44853/1‑149 (MQ=255)
       aaaaTAACGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTg                                                                                                                                               >  3:277074/1‑149 (MQ=255)
             aCGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTAc                                                                                                                                         <  7:66128/149‑1 (MQ=255)
              cGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTAc                                                                                                                                         >  2:351299/1‑148 (MQ=255)
                      aTTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTTCAAATCGttt                                                                                                                                <  3:226677/149‑1 (MQ=255)
                              cAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGttt                                                                                                                        >  6:263862/1‑149 (MQ=255)
                                 gACTATAGCGGATTCCCCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCg                                                                                                                     >  8:391393/1‑149 (MQ=255)
                                 gACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAg                                                                                                                                            <  2:176930/126‑1 (MQ=255)
                                 gACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCCAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAg                                                                                                                                            >  1:176930/1‑126 (MQ=255)
                                    tataGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGc                                                                                                                            >  5:159479/1‑139 (MQ=255)
                                    tataGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGc                                                                                                                            <  6:159479/139‑1 (MQ=255)
                                      taGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAACCAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAg                                                                                                                >  1:297334/1‑149 (MQ=255)
                                      taGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCTGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTa                                                                                                                 <  3:162895/148‑1 (MQ=255)
                                             ttCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAAt                                                                                                         >  7:71536/1‑149 (MQ=255)
                                                accctcacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACACACCTTCACCGAACAGACGACCAAACAGTTTCTTCAGCAGGTTCCCGAGTGGCCCGTGCTGGCGCTGGAGAACAAATCGTTACAGCGTTTTCGGGTAGCCAAAATTca                                                                                                      >  2:143289/1‑148 (MQ=255)
                                                  cctcacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTg                                                                                                    <  8:71536/149‑1 (MQ=255)
                                                    tcacccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGa                                                                                                   >  3:256341/1‑148 (MQ=255)
                                                      acccCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGa                                                                                                   <  4:256341/146‑1 (MQ=255)
                                                        cccGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATc                                                                                              >  7:261783/1‑149 (MQ=255)
                                                               gcgcAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGc                                                                                       >  7:157149/1‑149 (MQ=255)
                                                                   aaTCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAAc                                                                                   <  8:261783/149‑1 (MQ=255)
                                                                        ccGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGTTAGCCAAAATTCTTGACGATCTATTTGCGAACAAGCt                                                                              >  8:411133/1‑149 (MQ=255)
                                                                                 ctgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGaa                                                                                    >  8:43860/1‑134 (MQ=255)
                                                                                 ctgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCAtt                                                                     >  2:281199/1‑149 (MQ=255)
                                                                                  tgGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTg                                                                    >  7:92340/1‑149 (MQ=255)
                                                                                       cTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGtt                                                               <  1:351299/149‑1 (MQ=255)
                                                                                        tCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGCCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACCATCTATCTGCTAACCAGCAGCAACCATTGCTGtt                                                               >  2:220090/1‑148 (MQ=255)
                                                                                          aCCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTg                                                              >  8:81737/1‑147 (MQ=255)
                                                                                          aCCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGa                                                             <  7:391393/148‑1 (MQ=255)
                                                                                          aCCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCACGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTg                                                              <  6:290097/147‑1 (MQ=255)
                                                                                           ccTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAg                                                           <  1:281199/149‑1 (MQ=255)
                                                                                            cTTCTCCGAACAGACGACGAAACAGTTTCTTGAGCAGGGTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATGGTTTCTGCGGTTTCGGGTATCCAAAATTCTTGACGATCTAtgt                                                                                         <  6:388721/118‑1 (MQ=255)
                                                                                            cTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACTatatatct                                                                                         >  5:388721/1‑116 (MQ=255)
                                                                                            cTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTTCGAACCAGCTGCAACCATTGCTGtt                                                               >  8:374742/1‑144 (MQ=255)
                                                                                            cTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGa                                                          >  5:377310/1‑149 (MQ=255)
                                                                                            cTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGa                                                          >  3:243979/1‑149 (MQ=255)
                                                                                            cTTCACCGAACAGACGAACAAACAGTTTCTTTAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATTTGCGAACCATCTGCAACCATTGCTGtt                                                               <  7:374742/144‑1 (MQ=255)
                                                                                               cACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTAtgt                                                                                         <  6:84635/115‑1 (MQ=255)
                                                                                               cACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTAtgt                                                                                         >  5:84635/1‑115 (MQ=255)
                                                                                               cACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGAc                                                         <  7:81737/147‑1 (MQ=255)
                                                                                                aCCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGtt                                                      >  2:215705/1‑149 (MQ=255)
                                                                                                 ccGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTg                                                                 >  5:355594/1‑137 (MQ=255)
                                                                                                 ccGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTg                                                                 <  6:355594/137‑1 (MQ=255)
                                                                                                   gAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGAt                                                                                               >  7:118016/1‑105 (MQ=255)
                                                                                                   gAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGAt                                                                                               <  8:118016/105‑1 (MQ=255)
                                                                                                     aCAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCt                                                                              <  1:96155/120‑1 (MQ=255)
                                                                                                     aCAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCt                                                                              >  2:96155/1‑120 (MQ=255)
                                                                                                      cAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTaaa                                                <  8:157149/149‑1 (MQ=255)
                                                                                                      cAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTaaa                                                <  7:339831/149‑1 (MQ=255)
                                                                                                         acgacCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACcg                                             >  7:167465/1‑149 (MQ=255)
                                                                                                           gacCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCtt                                                                                                     >  8:153490/1‑91 (MQ=255)
                                                                                                           gacCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCtt                                                                                                     <  7:153490/91‑1 (MQ=255)
                                                                                                           gacCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACcgcg                                           >  3:308105/1‑149 (MQ=255)
                                                                                                            acCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCgcgc                                          <  3:44853/149‑1 (MQ=255)
                                                                                                              cAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCgcgcg                                         >  2:157141/1‑147 (MQ=255)
                                                                                                              cAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCt                                         >  4:63876/1‑148 (MQ=255)
                                                                                                              cAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCt                                         >  6:36041/1‑148 (MQ=255)
                                                                                                              cAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTGCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCGATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGGTGCTAAACCGCGCt                                         >  2:70246/1‑148 (MQ=255)
                                                                                                                aaCAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTg                                        >  1:183571/1‑147 (MQ=255)
                                                                                                                aaCAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGaa                                      <  2:183571/149‑1 (MQ=255)
                                                                                                                 aCAGTTTCTTGAGCAGGTTGCCGAGTGGCACGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTATTGACGATCTATGTGCGAACCAGCTGCAAACATTGCTGTTGAAGACGTTGCTAAACCGCGATGAAg                                     <  5:320943/149‑1 (MQ=255)
                                                                                                                   aGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGt                                   >  4:277095/1‑149 (MQ=255)
                                                                                                                   aGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGt                                   >  8:232973/1‑149 (MQ=255)
                                                                                                                     tttCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGc                                 <  5:78473/149‑1 (MQ=255)
                                                                                                                     tttCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGc                                 >  5:115740/1‑149 (MQ=255)
                                                                                                                     tttCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGc                                 >  1:411542/1‑149 (MQ=255)
                                                                                                                     tttCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGc                                 <  4:277074/149‑1 (MQ=255)
                                                                                                                       tCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCg                                <  3:277095/148‑1 (MQ=255)
                                                                                                                          tGAGCAGGTTGCCCAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTACCCAAAATTCTTGACGATCTATGTGAGAACCACCTCCAACCATTGCTGATGAAGACGTTGCTAAACCGCGCTGAAGGTGCgttgt                            >  4:233418/1‑149 (MQ=255)
                                                                                                                           gAGCAGGTTGCCGAGAGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGGGCgttgtt                           >  2:162303/1‑149 (MQ=255)
                                                                                                                            aGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCgttgtt                           <  8:167465/148‑1 (MQ=255)
                                                                                                                             gCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGa                         <  1:215705/149‑1 (MQ=255)
                                                                                                                                 gTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTaaa                                                >  5:291508/1‑122 (MQ=255)
                                                                                                                                 gTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTaaa                                                <  6:291508/122‑1 (MQ=255)
                                                                                                                                 gTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCaa                     >  6:210520/1‑149 (MQ=255)
                                                                                                                                  ttGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGAGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGATGATCaag                    >  6:303729/1‑148 (MQ=255)
                                                                                                                                      cGAGTGGCCCGTGCTGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAAGCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCgg                <  5:85565/149‑1 (MQ=255)
                                                                                                                                      cGAGTGGCCCGTGCTGGCGCTGGAGTACAAATCGTTACTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTCCTGTTGAAGACGTTGCTAAAACGCTCAGAAGGTGCGTTGTTGATCAATGCgg                >  6:85565/1‑149 (MQ=255)
                                                                                                                                      cGAGTGGCCCGTGCAGGCGCTGCAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAACCCGCGCTGAAGGAGCGTTGTTGATCAATGCgg                >  6:281085/1‑149 (MQ=255)
                                                                                                                                       gAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCgttg                             <  1:49007/135‑1 (MQ=255)
                                                                                                                                       gAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCgttg                             >  2:49007/1‑135 (MQ=255)
                                                                                                                                         gTGGCCCGTGCATGCGCTGGAGTTCATATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTCTGTGGGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCggtgg             <  1:220090/149‑1 (MQ=255)
                                                                                                                                             cccGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGgtgt         >  5:324080/1‑149 (MQ=255)
                                                                                                                                                gTGCAGGCGCTGGAGTAGTAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTGTTGACGATGTATGTGCGAACCAGCTGCATCCTTTGCTGTTGAAGACGTTGCTATACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCga      <  1:143289/149‑1 (MQ=255)
                                                                                                                                                gTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCga      >  1:269168/1‑149 (MQ=255)
                                                                                                                                                gTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCga      <  7:411133/149‑1 (MQ=255)
                                                                                                                                                    aGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCgatgat  <  4:308105/149‑1 (MQ=255)
                                                                                                                                                    aGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCgatgat  <  6:324080/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGTACAAAATAACGCTGTCGATTCAGGCCAGGACTATAGCGGATTCACCCTCACCCCGTCGGCGCAATCCCCGCGTCTGCTGGAACTCACCTTCACCGAACAGACGACCAAACAGTTTCTTGAGCAGGTTGCCGAGTGGCCCGTGCAGGCGCTGGAGTACAAATCGTTTCTGCGTTTTCGGGTAGCCAAAATTCTTGACGATCTATGTGCGAACCAGCTGCAACCATTGCTGTTGAAGACGTTGCTAAACCGCGCTGAAGGTGCGTTGTTGATCAATGCGGTGGGTGTCGATGAT  >  NC_000913/2789000‑2789296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: