Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,154,920 G→A 5.4% P100L (CCG→CTG)  yqhC ← transcriptional activator of yqhD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,154,9200GA 5.4% 236.3 / 2.6 74P100L (CCG→CTG) yqhCtranscriptional activator of yqhD
Reads supporting (aligned to +/- strand):  ref base G (31/39);  new base A (2/2);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01

AATCCCGCTGGCTGCCATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACA  >  NC_000913/3154772‑3155064
                                                                                                                                                    |                                                                                                                                                
aaTCCCGCTGGCTGCCATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCAACAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAaacggcaacg                                                                                                                                                  >  2:210098/1‑149 (MQ=255)
 aTCCCGCTGGCTGCCATCGACGTCTGGAAATGATCATCTTCGCCAATGTCCATCAACAGTTCCTTTAACTGCAAAATATCGACATTGAGACGCAACCCTGCAAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAaacggcaacg                                                                                                                                                  <  7:318998/148‑1 (MQ=255)
       ctggctgCCATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGt                                                                                                                                           <  4:382770/149‑1 (MQ=255)
          gctgCCATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcag                                                                                                                                        <  5:232192/149‑1 (MQ=255)
            tgCCATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcag                                                                                                                                        >  2:234626/1‑147 (MQ=255)
              ccATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcagc                                                                                                                                    <  3:128610/149‑1 (MQ=255)
              ccATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcag                                                                                                                                     <  1:20387/148‑1 (MQ=255)
               cATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcag                                                                                                                                     >  1:401565/1‑147 (MQ=255)
                aTCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcagcag                                                                                                                                  <  2:401565/149‑1 (MQ=255)
                aTCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcagcag                                                                                                                                  <  6:221682/149‑1 (MQ=255)
                       gCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCa                                                                                                                           <  3:370653/149‑1 (MQ=255)
                         tGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCAtt                                                                                                                         >  5:345287/1‑149 (MQ=255)
                         tGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCAtt                                                                                                                         >  8:67208/1‑149 (MQ=255)
                           gAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTg                                                                                                                        <  3:58714/148‑1 (MQ=255)
                                gCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGcatca                                                                                                                  >  7:169137/1‑149 (MQ=255)
                                  taaTCTTCTCCAATGTCCATCAACAGTTCCTGTAACTGCAAAAGATCGCCATTGAGCCGCAACACTGCCAGCGGCACCTCTGAAGTGTCATAGGGTTCGCACTCAACCCGCACCGGCCCCGTCAGACGCAGTGATTCATAGGcatcat                                                                                                                 >  5:121560/3‑148 (MQ=255)
                                  tCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGcatcat                                                                                                                 >  6:198979/1‑148 (MQ=255)
                                  tCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGcatcat                                                                                                                 >  3:112427/1‑148 (MQ=255)
                                   cATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATaa                                                                                                               >  8:18220/1‑149 (MQ=255)
                                    aTCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATaa                                                                                                               <  8:312387/148‑1 (MQ=255)
                                       ttCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGa                                                                                                            >  1:130074/1‑148 (MQ=255)
                                         cGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTTCCAGCGGCACCTCTGACGTGGCATAGGTTTCTCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACg                                                                                                             <  3:256868/145‑1 (MQ=255)
                                           ccAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGGGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAacac                                                                                                       <  5:346220/149‑1 (MQ=255)
                                              aTGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTAt                                                                                                                              <  5:30567/123‑1 (MQ=255)
                                              aTGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTAt                                                                                                                              >  6:30567/1‑123 (MQ=255)
                                               tGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGt                                                                                                   <  2:130074/149‑1 (MQ=255)
                                                 tCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTc                                                                                                 <  4:112427/149‑1 (MQ=255)
                                                   cATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCAtt                                                                                                                         <  4:64740/123‑1 (MQ=255)
                                                   cATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCAtt                                                                                                                         >  3:64740/1‑123 (MQ=255)
                                                   cATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCAt                                                                                               <  7:67208/149‑1 (MQ=255)
                                                   cATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCAt                                                                                               >  8:187464/1‑149 (MQ=255)
                                                    aTCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTca                                                                                                                                         <  3:200631/106‑1 (MQ=255)
                                                    aTCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTca                                                                                                                                         >  4:200631/1‑106 (MQ=255)
                                                     tCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCAtt                                                                                              >  1:197468/1‑148 (MQ=255)
                                                        aCAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGata                                                                                          <  1:234626/149‑1 (MQ=255)
                                                          aGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGatat                                                                                         >  1:41481/1‑148 (MQ=255)
                                                          aGTTCCTGTAACTGCAAAATATCGACATT‑‑GACGCAACCCTGCCAGCGGCACCTCTTACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATAAAAc                                                                                      <  6:4566/149‑1 (MQ=255)
                                                            gtCCTGTAACTGCAAAATATCGACCTTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAAc                                                                                      <  2:83375/148‑1 (MQ=255)
                                                                tGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGAt                                                                                  >  7:309403/1‑149 (MQ=255)
                                                                     cTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCAt                                                                                               <  3:372994/131‑1 (MQ=255)
                                                                      tGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcagc                                                                                                                                    <  4:106513/93‑1 (MQ=255)
                                                                      tGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTcagcagc                                                                                                                                    >  3:106513/1‑93 (MQ=255)
                                                                      tGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCAtt                                                                                              >  8:14181/1‑131 (MQ=255)
                                                                      tGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCAtt                                                                                              <  7:14181/131‑1 (MQ=255)
                                                                        cAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTTAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGcc                                                                          >  1:144675/1‑149 (MQ=255)
                                                                        cAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGAtttt                                                                               >  1:164718/1‑144 (MQ=255)
                                                                        cAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGAtttt                                                                               <  2:164718/144‑1 (MQ=255)
                                                                          aaaTATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCg                                                                        >  1:359597/1‑149 (MQ=255)
                                                                               tCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGaaaa                                                                   <  2:359597/149‑1 (MQ=255)
                                                                               tCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGaaaa                                                                   <  1:210098/149‑1 (MQ=255)
                                                                               tCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGaa                                                                     >  6:157000/1‑147 (MQ=255)
                                                                                    aTTGAGACGCAACCCTGCCAGCGGCACCTCTTACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGaa                                                              <  1:53674/149‑1 (MQ=255)
                                                                                         gaCGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTatg                                                         <  2:41481/149‑1 (MQ=255)
                                                                                          aCGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTTAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTatga                                                        <  2:144675/149‑1 (MQ=255)
                                                                                               aCCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCa                                                   <  6:345287/149‑1 (MQ=255)
                                                                                                cccTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAg                                                  <  5:157000/149‑1 (MQ=255)
                                                                                                 ccTTCCAGCGGCACCTCTGACGTGGCATAGGTTTCGACCTCAAACGGCCACGGCACCGTCAGAAGCAGGTATTCATTGGCATCATAACGAACCACGCTTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAgg                                                 <  6:121560/149‑1 (MQ=255)
                                                                                                           gCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCACCGGCACCGTCAGCAGCAGGAATTCATTGGCATCATAAAGAAACCCGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCAAGGCACGTACAt                                        >  1:306155/1‑148 (MQ=255)
                                                                                                            cACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCa                                      >  7:257080/1‑149 (MQ=255)
                                                                                                               ctctGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCg                                   >  1:382976/1‑149 (MQ=255)
                                                                                                                   gACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTa                                           >  4:262834/1‑137 (MQ=255)
                                                                                                                   gACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTa                                           <  3:262834/137‑1 (MQ=255)
                                                                                                                    aCGTGGCATAGGTTTCGCACTCAAACGGCAACAGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATTTAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGgtgt                               <  8:354276/148‑1 (MQ=255)
                                                                                                                    aCGTGGCATAGGTTTCGCACTCAAACGGCAACAGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATTTAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGgtgt                               >  7:354276/1‑148 (MQ=255)
                                                                                                                      gTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTa                                           <  3:180533/134‑1 (MQ=255)
                                                                                                                           aTAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATACCCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCg                        >  5:325282/1‑148 (MQ=255)
                                                                                                                            tAGGTTTCGCACTCAAACGGCAACAGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGt                           >  8:268980/1‑144 (MQ=255)
                                                                                                                            tAGGTTTCGCACTCAAACGGCAACAGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGt                           <  7:268980/144‑1 (MQ=255)
                                                                                                                              ggTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGaaa                     <  5:198979/148‑1 (MQ=255)
                                                                                                                              ggTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAg                    <  7:18220/149‑1 (MQ=255)
                                                                                                                               gTTTCGAACTCAAACGGCAACGGCACAGTCAGGAGCAGGTATTCATTGGCATCATAACGAAACTCGCTTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATTCCAGGCGCGTACATAACCGGTGTACGTGCGAAAg                    <  2:306155/148‑1 (MQ=255)
                                                                                                                                tttCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGc                  >  8:138179/1‑149 (MQ=255)
                                                                                                                                 ttCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCg                 >  5:299816/1‑149 (MQ=255)
                                                                                                                                     cACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGtctc             <  8:169137/149‑1 (MQ=255)
                                                                                                                                      aCTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGAATTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCCGTGTACGAGCGAGAGGGGtaaag            >  1:51116/1‑145 (MQ=255)
                                                                                                                                       cTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGc           <  6:299816/149‑1 (MQ=255)
                                                                                                                                               gcaacggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaa    >  1:121221/1‑148 (MQ=255)
                                                                                                                                                 aacggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATacaaca  >  8:227582/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AATCCCGCTGGCTGCCATCGACGGCTGGAAATGCTCATCTTCGCCAATGTCCATCAACAGTTCCTGTAACTGCAAAATATCGACATTGAGACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACA  >  NC_000913/3154772‑3155064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: