| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,161,358 | G→C | 5.1% | Q438E (CAG→GAG) | ftsP ← | septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,161,358 | 0 | G | C | 5.1% | 267.6 / 4.5 | 78 | Q438E (CAG→GAG) | ftsP | septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts) |
| Reads supporting (aligned to +/- strand): ref base G (37/37); new base C (2/2); total (39/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
ATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCG > NC_000913/3161212‑3161505 | aTCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGAc < 8:323850/149‑1 (MQ=255) tCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCACTCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGAcc < 6:19591/149‑1 (MQ=255) aaaGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGGCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGaa < 6:369327/149‑1 (MQ=255) aGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAAt < 6:142887/149‑1 (MQ=255) gTAATCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATaa < 7:89105/149‑1 (MQ=255) ttCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCTCGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTCACCGAAATAAAc > 3:272688/1‑148 (MQ=255) ttCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATGTCCAGCGTCGGACTGTTGAAGTAGCACGGGAAGTGCGCCCCGGAAGGCTCACCGAAATAAAc > 3:267620/1‑148 (MQ=255) tCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGTCCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAAc > 7:148118/1‑147 (MQ=255) cATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGTATTGAACAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagc < 8:380802/149‑1 (MQ=255) cATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGTCCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagc < 8:148118/149‑1 (MQ=255) aTACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCTTATTGACCAACAGTTGCCCAATCGAGACACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCATGAAGGCTGACCGAAATAAACAagca < 1:207586/149‑1 (MQ=255) tACTCGCCCTCCGCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGCGCCCCGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagcag > 3:228979/1‑149 (MQ=255) tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTc > 7:53033/1‑149 (MQ=255) cctcctCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACt > 5:326399/1‑149 (MQ=255) ctcctcGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACtt < 2:79463/149‑1 (MQ=255) tcctcGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTg > 7:77803/1‑149 (MQ=255) ctcGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCATTCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGt < 2:166040/148‑1 (MQ=255) cGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCaa < 8:137989/149‑1 (MQ=255) aGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACaa > 3:45049/1‑126 (MQ=255) aGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACaa < 4:45049/126‑1 (MQ=255) aGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAAcc > 7:298936/1‑149 (MQ=255) gAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAAc > 1:228785/1‑149 (MQ=255) aTTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTa > 6:130097/1‑149 (MQ=255) aTTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTa < 6:326399/149‑1 (MQ=255) ttACGGTACCGGATTGACCAAGAGTTGCCCAATCGAGCCACGTGACGCCATTTCCATCGTCTGACTGTTGAAGTAGAACTGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTAt < 5:218958/149‑1 (MQ=255) tACTGTACCGGATTGACCAACAGTTGCGCAATCGAGCCACGGTCAGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCTCCCAGGAAGGCTGACCGATATACACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTag < 6:31385/148‑2 (MQ=255) cGGTACCGGATGGACCACCAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCGGACCGAAATAAACCAGCAGCTCCACTTGTCCGGCAACCCAAACGGTATCtt < 4:228979/149‑1 (MQ=255) ggTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCttt < 8:228531/149‑1 (MQ=255) gATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCct > 2:314442/1‑149 (MQ=255) gACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTg < 8:53033/148‑1 (MQ=255) aCCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTc < 1:372515/149‑1 (MQ=255) ccAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCt < 8:77803/149‑1 (MQ=255) cAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCACCCCAAACGGTATCTTTCCAGCCTCTGTCtt > 6:134361/1‑149 (MQ=255) aaCAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTc > 8:276411/1‑149 (MQ=255) cAGTTGCCCAATCGATCGACGGTCCTCCATTTCAAGCGTATGACTGTTGAATTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCACCCCAAACGGTATCTTTCCAGCCTCTGTCTTCCg < 8:337232/149‑1 (MQ=255) ttGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCggg > 2:152534/1‑148 (MQ=255) cccAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACg < 1:152534/149‑1 (MQ=255) ccAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAACTAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACgg > 1:190770/1‑149 (MQ=255) aaTCGCGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAACCGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCa > 4:83042/1‑149 (MQ=255) aTCGAGCCACGGTCCGCGATTTCCAGCGTCTGACTGTTGAAGTAGAACGTGAAGTGCGCCCAGGAAGGCTGACGGAAATAAACTAGCAGCTCCACTTGTCCGTCAACCAAAACGGTATCTTTCCAGCCTCTGTGTTCCGGGAACg < 1:365882/145‑1 (MQ=255) aTCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCAt > 1:326227/1‑149 (MQ=255) aTCGAGCCACGGTCCGCCATTTCCAGCGACTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGAAAGGCTGACCGAAATAAACAAGCAGCTCCACTTTTCCGTCAACCCAAACGGTAACTTTCCAGCCTCTCTCTTCCGCGAACg > 2:365882/1‑145 (MQ=255) aCGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCATGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCAt < 5:134361/149‑1 (MQ=255) ccATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTa > 1:136651/1‑149 (MQ=255) cATTTCCAGCGTCTGACTGTTTAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTAc > 5:197887/1‑149 (MQ=255) aTTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACg > 8:23570/1‑149 (MQ=255) cAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCt < 3:83042/149‑1 (MQ=255) gCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTg > 8:145808/1‑148 (MQ=255) cTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACa > 4:117582/1‑149 (MQ=255) cTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACa > 4:112407/1‑149 (MQ=255) tGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACg > 1:36844/1‑149 (MQ=255) ttGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGaa > 1:284419/1‑140 (MQ=255) gAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAAc < 1:137265/139‑1 (MQ=255) gAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAAc > 2:137265/1‑139 (MQ=255) aaGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCtt > 1:374023/1‑148 (MQ=255) gTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCaa > 8:304189/1‑149 (MQ=255) gAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAatat < 6:197887/149‑1 (MQ=255) gAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAatat > 4:96415/1‑149 (MQ=255) aaCGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATg < 1:407233/149‑1 (MQ=255) aCGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATgg < 1:314442/149‑1 (MQ=255) cGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATgg > 2:76954/1‑148 (MQ=255) gggAAGTGCGCCCAGGAAGGCTCACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGaa < 4:272688/149‑1 (MQ=255) gggAAGTGCGCCCAGGAAGGCTCACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGGCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGaa < 4:267620/149‑1 (MQ=255) ggAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAAc < 8:298936/149‑1 (MQ=255) aGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCt > 7:103894/1‑149 (MQ=255) gcgcCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGc < 3:112407/149‑1 (MQ=255) cgcCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGc < 3:117582/148‑1 (MQ=255) cgcCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCg < 3:96415/149‑1 (MQ=255) gcCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCgg > 1:153131/1‑149 (MQ=255) gcCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCgg > 7:102332/1‑149 (MQ=255) cAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATgg > 4:177696/1‑135 (MQ=255) cAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATgg < 3:177696/135‑1 (MQ=255) cAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTc < 2:326227/149‑1 (MQ=255) gAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCa > 8:45707/1‑62 (MQ=255) gAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCa < 7:45707/62‑1 (MQ=255) gCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGc > 2:361983/1‑137 (MQ=255) gCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGc < 1:361983/137‑1 (MQ=255) tGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCcgcgcg > 1:114378/1‑149 (MQ=255) gACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGaa < 8:276162/106‑1 (MQ=255) gACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGaa > 7:276162/1‑106 (MQ=255) | ATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTCCGTCAACCCAAACGGTATCTTTCCAGCCTCTGTCTTCCGGGAACGGCATCGCGCCATTCACGTTACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCG > NC_000913/3161212‑3161505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |