Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,564,528 C→T 6.2% T54T (ACG→ACA)  yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,564,5280CT 6.2% 311.9 / 6.5 97T54T (ACG→ACA) yjiLputative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehydratase
Reads supporting (aligned to +/- strand):  ref base C (46/45);  new base T (3/3);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCAATGCCAA  >  NC_000913/4564380‑4564676
                                                                                                                                                    |                                                                                                                                                    
cTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGc                                                                                                                                                      <  6:171630/149‑1 (MQ=255)
 tttGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCg                                                                                                                                                     >  7:216062/1‑149 (MQ=255)
   tGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCATAAACGGCg                                                                                                                                                     <  5:201841/147‑1 (MQ=255)
    gCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGtt                                                                                                                                                   <  1:8112/148‑1 (MQ=255)
        tCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGtctc                                                                                                                                              >  6:198437/1‑149 (MQ=255)
        tCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGtctc                                                                                                                                              >  8:180197/1‑149 (MQ=255)
        tCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGAGTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGtct                                                                                                                                               >  4:312711/1‑148 (MQ=255)
          cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGTGTTGTctct                                                                                                                                             >  7:366578/1‑148 (MQ=255)
          cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGTGTTGTctct                                                                                                                                             >  3:71889/1‑148 (MQ=255)
          cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGTGTTGTctct                                                                                                                                             >  3:76532/1‑148 (MQ=255)
          cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTctct                                                                                                                                             <  7:270911/148‑1 (MQ=255)
          cTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTa                                                                                                                                            <  8:216062/149‑1 (MQ=255)
           tGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTaa                                                                                                                                           >  1:123259/1‑149 (MQ=255)
             accaccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTaa                                                                                                                                           <  7:39168/147‑1 (MQ=255)
             accaccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAAcc                                                                                                                                         >  7:304513/1‑149 (MQ=255)
               caccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCt                                                                                                                                       >  5:350256/1‑149 (MQ=255)
                 ccGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTc                                                                                                                                     <  6:134952/149‑1 (MQ=255)
                  cGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTcg                                                                                                                                    >  7:252840/1‑149 (MQ=255)
                         gATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCaga                                                                                                                              <  2:123259/148‑1 (MQ=255)
                              ccGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCCGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTcc                                                                                                                        >  6:278895/1‑149 (MQ=255)
                               cgcgctcgTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTccc                                                                                                                       <  6:156145/149‑1 (MQ=255)
                               cgcgcgcgTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTccc                                                                                                                       <  2:219227/149‑1 (MQ=255)
                                gcgcgcgTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGTGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCa                                                                                                                      <  4:76532/149‑1 (MQ=255)
                                gcgcgcgTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGTGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCa                                                                                                                      <  4:71889/149‑1 (MQ=255)
                                gcgcgcgTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGTGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCa                                                                                                                      <  8:366578/149‑1 (MQ=255)
                                     cgTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCtt                                                                                                                 <  7:286163/149‑1 (MQ=255)
                                      gTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAATCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCtt                                                                                                                 >  3:264129/1‑148 (MQ=255)
                                       ttGCTGGCGCAAGAAACAGGGGGCCCAGCCCGGGACTGGAGATTTCCGTTACCTGTTTATGGGCAAAATCCTCAAGTTTCCGCCCGTAGCCGGTGAGCGTCTGAAACGTCGTTGTCTCTATCCCTTCGCGCTGAGTTTCCCAGGCTTCa                                                                                                               <  2:268939/149‑1 (MQ=255)
                                           tGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTaa                                                                                                           >  2:313872/1‑149 (MQ=255)
                                             gcgcAAGCAACCGTGAGACAAGCCCGAGACAGGAGATTTCCGTTACCTGGTTATCGGCAAAATCCACCAGTTGCAGCCCGTAGCCGGGGAGCGGCAGAAACGGCGTTGTCTCTAACCCTTCGCGAAGAGTTTCCCAGGCTTCAGTaa                                                                                                           <  6:262671/147‑1 (MQ=255)
                                             gcgcAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAAt                                                                                                          <  8:252840/148‑1 (MQ=255)
                                                cAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCt                                                                                                      <  5:104369/149‑1 (MQ=255)
                                                 aaGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACGAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTATATCCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTg                                                                                                     <  2:181819/149‑1 (MQ=255)
                                                  aGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGt                                                                                                    <  7:146112/149‑1 (MQ=255)
                                                        cgatcgcCCAGCCCGTGACAGGAGATTTCCGTTACCTTTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTTAGCGTCAGAAACGGCGTTTTCTCTAACACTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCgg                                                                                              <  6:72880/145‑1 (MQ=255)
                                                        cGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCgg                                                                                              >  6:327291/1‑149 (MQ=255)
                                                            cgcCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCg                                                                                           >  3:332076/1‑148 (MQ=255)
                                                               ccAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGTCGAAAg                                                                                       >  1:227751/1‑149 (MQ=255)
                                                                  gCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCACAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAgggg                                                                                    <  3:312711/149‑1 (MQ=255)
                                                                   cccGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAggggg                                                                                   >  4:245999/1‑149 (MQ=255)
                                                                      gTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTg                                                                                <  7:180197/149‑1 (MQ=255)
                                                                           aGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCa                                                                                                               >  2:249489/1‑113 (MQ=255)
                                                                           aGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCa                                                                                                               <  1:249489/113‑1 (MQ=255)
                                                                            ggAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGACACGGCGTTGTCTCTAACCCTTCGCGCAGCGTTTCGCAGGCTTCAGTATTTGCTGTTGCGGGGCGATAGGGGGTTGGAAc                                                                            >  4:155581/1‑147 (MQ=255)
                                                                            ggAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTCGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAAc                                                                            >  6:53861/1‑147 (MQ=255)
                                                                             gagaTTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAg                                                                         <  5:198437/149‑1 (MQ=255)
                                                                                  ttCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAAc                                                                    >  1:52181/1‑149 (MQ=255)
                                                                                   tCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAAc                                                                    <  4:25503/148‑1 (MQ=255)
                                                                                   tCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACg                                                                   >  7:144480/1‑149 (MQ=255)
                                                                                     cGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGgc                                                                 <  5:62367/149‑1 (MQ=255)
                                                                                     cGTTACCTGTTTATCGGCAAAATCCACCAGTTGACGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGgc                                                                 <  7:113780/149‑1 (MQ=255)
                                                                                      gTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGgcg                                                                >  6:311805/1‑149 (MQ=255)
                                                                                            tGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAAt                                                          >  6:132841/1‑149 (MQ=255)
                                                                                                 aTCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACg                                                      <  2:52181/148‑1 (MQ=255)
                                                                                                  tCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAg                                                                                       <  4:301283/114‑1 (MQ=255)
                                                                                                  tCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAg                                                                                       >  3:301283/1‑114 (MQ=255)
                                                                                                  tCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGcc                                                    <  5:278895/149‑1 (MQ=255)
                                                                                                    ggCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGt                                                  >  6:328701/1‑149 (MQ=255)
                                                                                                    ggCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGt                                                  <  2:227751/149‑1 (MQ=255)
                                                                                                    ggCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGt                                                  <  4:290938/149‑1 (MQ=255)
                                                                                                    ggCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGt                                                  >  8:336745/1‑149 (MQ=255)
                                                                                                     gCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTc                                                 >  6:139402/1‑149 (MQ=255)
                                                                                                      cAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCt                                                >  1:17798/1‑149 (MQ=255)
                                                                                                          aTCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCa                                            <  8:144480/149‑1 (MQ=255)
                                                                                                           tCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAg                                           >  5:119748/1‑149 (MQ=255)
                                                                                                            ccaccaGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAg                                           >  8:318584/1‑148 (MQ=255)
                                                                                                             caccaGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAgg                                                                                      <  5:29202/104‑1 (MQ=255)
                                                                                                             caccaGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAgg                                                                                      >  6:29202/1‑104 (MQ=255)
                                                                                                              accaGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGt                                          >  4:4193/1‑147 (MQ=255)
                                                                                                              accaGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGATGTCTCAAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTaa                                        >  7:7057/1‑149 (MQ=255)
                                                                                                                caGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGt                                          >  1:332734/1‑145 (MQ=255)
                                                                                                                     gccgccCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCt                                 >  8:330977/1‑149 (MQ=255)
                                                                                                                      ccgccCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCtt                                >  7:106243/1‑149 (MQ=255)
                                                                                                                      ccgccCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCtt                                <  4:264129/149‑1 (MQ=255)
                                                                                                                         cccGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTtgg                             >  1:245379/1‑149 (MQ=255)
                                                                                                                         cccGTAGCCCGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCGGGAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCAAGCCGTCTGCCAGTAAGATCCCttttg                             >  4:242915/1‑147 (MQ=255)
                                                                                                                               gCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGGTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCgg                       <  7:336745/149‑1 (MQ=255)
                                                                                                                                ccGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGt                      >  3:158141/1‑149 (MQ=255)
                                                                                                                                ccGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGt                      <  7:318584/149‑1 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAAGCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGGTGTTGCCGGGCGAAAGGGGGTTGGATCGAGGAAACGCCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGtt                     >  3:148994/1‑149 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTaa                                                           <  2:232578/111‑1 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTaa                                                           >  1:232578/1‑111 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTtggt                            <  1:163495/142‑1 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTtggt                            >  2:163495/1‑142 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGtt                     <  3:4193/149‑1 (MQ=255)
                                                                                                                                 cGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCCAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGCTGGCGGtt                     >  3:154374/1‑149 (MQ=255)
                                                                                                                                  ggTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTg                    <  1:313872/149‑1 (MQ=255)
                                                                                                                                   gTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGtt                     >  8:69440/1‑147 (MQ=255)
                                                                                                                                      aGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGc                 <  5:139402/148‑1 (MQ=255)
                                                                                                                                         gTCAGAAACGGCGTAGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGCACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCgg              >  4:250655/1‑148 (MQ=255)
                                                                                                                                            aGAAACGGCGTCGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAAt           <  5:53861/148‑1 (MQ=255)
                                                                                                                                              aaaCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCaa        <  5:327291/149‑1 (MQ=255)
                                                                                                                                              aaaCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCaa        <  2:332734/149‑1 (MQ=255)
                                                                                                                                               aaCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCAAt       <  4:332076/149‑1 (MQ=255)
                                                                                                                                               aaCGGCGTTGACTCTAACCCTTCGCGCAGAGTTTCCCAGGCTGCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTACGATCCCTTTGGTGGCGGTTGAGCCGGAATCAAt       <  6:350256/149‑1 (MQ=255)
                                                                                                                                                aCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACg                                                                           >  4:5106/1‑80 (MQ=255)
                                                                                                                                                aCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACg                                                                           <  3:5106/80‑1 (MQ=255)
                                                                                                                                                aCGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCAATg      >  7:119581/1‑149 (MQ=255)
                                                                                                                                                    cGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCAATGCCaa  >  6:335284/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTTTGCTGTCCTGACCACCGATGTCGATTACCGCGCGCGTTGCTGGCGCAAGAAACCGTGCGCCCAGCCCGTGACAGGAGATTTCCGTTACCTGTTTATCGGCAAAATCCACCAGTTGCCGCCCGTAGCCGGTGAGCGTCAGAAACGGCGTTGTCTCTAACCCTTCGCGCAGAGTTTCCCAGGCTTCAGTAATTGCTGTTGCCGGGCGAAAGGGGGTTGGAACGAGGAAACGGCGCGTAATCACGCCGTCTGCCAGTAAGATCCCTTTGGTGGCGGTTGAGCCGGAATCAATGCCAA  >  NC_000913/4564380‑4564676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑