Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,394,083 C→T 6.3% T286M (ACG→ATG)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,394,0830CT 6.3% 245.4 / 3.4 78T286M (ACG→ATG) mppAmurein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (40/33);  new base T (3/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

CTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTG  >  NC_000913/1393941‑1394230
                                                                                                                                              |                                                                                                                                                   
cTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccgcc                                                                                                                                               >  5:31088/1‑149 (MQ=255)
 tGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccgccg                                                                                                                                              >  3:178205/1‑149 (MQ=255)
 tGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccgccg                                                                                                                                              >  1:337410/1‑149 (MQ=255)
   cAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccg                                                                                                                                                 >  3:325578/1‑144 (MQ=255)
   cAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATAcgccg                                                                                                                                                 <  4:325578/144‑1 (MQ=255)
        aGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCg                                                                                                                                       >  2:126160/1‑149 (MQ=255)
          tGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCggg                                                                                                                                     <  2:337410/149‑1 (MQ=255)
            aCCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATATGCCGCCGCAGCTCGGGAc                                                                                                                                   >  5:275358/1‑149 (MQ=255)
            aCCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATATGCCGCCGCAGCTCGGGAc                                                                                                                                   >  5:280432/1‑149 (MQ=255)
                  cTGCCAATTAATGAGGAATCCGCAGCCACTAAGCGTTACGTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGACGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtatta                                                                                                                             <  8:370718/149‑1 (MQ=255)
                  cTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtatta                                                                                                                             <  4:245306/149‑1 (MQ=255)
                    gCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCAGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtattat                                                                                                                            >  2:97136/1‑148 (MQ=255)
                     ccAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtattatta                                                                                                                          >  2:160161/1‑149 (MQ=255)
                      cAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtattattat                                                                                                                         >  7:243983/1‑149 (MQ=255)
                      cAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCtattattat                                                                                                                         <  8:165016/149‑1 (MQ=255)
                       aaTTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGACTCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATg                                                                                                                        >  4:156782/1‑149 (MQ=255)
                       aaTTAATCACGAATCCCCAGCCACTAAGCGTTACCTCGCGGGGGATCTTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGACGCTGGTGAAGGATATTCCGGGGCACGGTTATCCGCCGCCGCAGCTCGGGACCTATTATTATg                                                                                                                        <  2:35076/149‑1 (MQ=255)
                        aTTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGc                                                                                                                       >  2:268087/1‑149 (MQ=255)
                            aTCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGtt                                                                                                                    <  6:357963/148‑1 (MQ=255)
                            aTCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGtt                                                                                                                    >  1:332957/1‑148 (MQ=255)
                            aTCAGGAATCAGCAGCCAATAAGCGTTACCTCGAGGGGGAAATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGtt                                                                                                                    <  5:7614/148‑1 (MQ=255)
                               aGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTaa                                                                                                                 <  2:297669/148‑1 (MQ=255)
                               aGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAac                                                                                                                <  1:97136/149‑1 (MQ=255)
                                       gCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCATTCCCGAAAAATATGTAACAGAAGCTGTTGAAGCATATACCGGGGCAGGTTTATACTCCGCCGCAGCTCGGAACCTATTATTAAGCGTTTAACACGCaaa                                                                                                         >  8:334690/1‑148 (MQ=255)
                                            cACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATATGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGc                                                                                                   <  6:280432/149‑1 (MQ=255)
                                            cACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATATGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGc                                                                                                   <  6:275358/149‑1 (MQ=255)
                                            cACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAggg                                                                                                    <  4:178205/148‑1 (MQ=255)
                                             aCTAAGCGTTACCTCGCGGGGGATATTGATATCCCCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGcc                                                                                                  >  8:102374/1‑149 (MQ=255)
                                             aCTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGcc                                                                                                  <  1:160161/149‑1 (MQ=255)
                                               tAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGa                                                                                                >  1:212463/1‑149 (MQ=255)
                                                         cTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGAt                                                                                        >  1:286370/1‑147 (MQ=255)
                                                            gcgGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAgcg                                                                                   <  6:31088/149‑1 (MQ=255)
                                                            gcgGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAgc                                                                                    <  2:212463/148‑1 (MQ=255)
                                                                 ggATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTc                                                                              <  8:243983/149‑1 (MQ=255)
                                                                    tatTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAggg                                                                                                    >  3:7836/1‑124 (MQ=255)
                                                                    tatTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAggg                                                                                                    <  4:7836/124‑1 (MQ=255)
                                                                     atTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGCCAGATCAGCGCGTTCGTCt                                                                          >  8:195116/1‑149 (MQ=255)
                                                                      ttGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTg                                                                         >  3:48835/1‑149 (MQ=255)
                                                                        gATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTgg                                                                        <  1:186810/148‑1 (MQ=255)
                                                                         atatCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGt                                                                                <  7:38476/139‑1 (MQ=255)
                                                                         atatCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGt                                                                                >  8:38476/1‑139 (MQ=255)
                                                                           atCACCGAATCCTTCCCGAAATATATGTCTCCGAAGCTGTTGAAGGAAATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTATAACCCGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCAtt                                                                    <  2:349023/149‑1 (MQ=255)
                                                                           atCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGCCCTATTATTATGCGTTTAACACGCAAAAAGGGCCGCCGGCAGATCAGCGCGTTCGGCTGGCag                                                                     >  6:345984/1‑147 (MQ=255)
                                                                             cacagcaTCCTTCCCGAAAAATAAGTAACAGAACCTGTAGAAGTATATTCCCGGGCACGAAAATACACCTCCGCAGCTCGGGACCTATTATTATGCTTTTAACACGCAAAAAGGTCCGACGTCAGATCAGATATTTCGTCTGGCAtt                                                                    >  4:233712/7‑147 (MQ=255)
                                                                              tccGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAg                                                                 <  4:257990/148‑1 (MQ=255)
                                                                              aCCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTaa                                                                  <  1:126160/148‑1 (MQ=255)
                                                                              aCCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAg                                                                 <  3:253169/149‑1 (MQ=255)
                                                                               ccGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGGCCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAg                                                                 >  6:109418/1‑148 (MQ=255)
                                                                                cGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATATGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTa                                                               >  2:323285/1‑149 (MQ=255)
                                                                                cGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGGCCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTa                                                               <  5:109418/149‑1 (MQ=255)
                                                                                  aaTCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGCCGGCAGATCAGCGCGTTCGTCTGGCATTAAGTAt                                                              >  6:4283/1‑148 (MQ=255)
                                                                                  aaTCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATg                                                             >  7:154335/1‑149 (MQ=255)
                                                                                   aTCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGa                                                            <  4:48835/149‑1 (MQ=255)
                                                                                      cTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCTGGGCAGGTTTATACGCCGCAGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGTGCCGTCGGAAGATCCGCGCGTTCGTATGGCATTAAGTATGACgc                                                         >  5:103535/1‑148 (MQ=255)
                                                                                       ttCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCTGATCAGCGCGTTCGTCTGGCATTAAGTATGACGAt                                                        >  1:280326/1‑149 (MQ=255)
                                                                                            gAAAAATATGTATCAGTAGCTGTTGAATGATATTCCGTGGGAGGTTTATACGCCGCCGCAGCTCGGTACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATc                                                   <  7:334690/149‑1 (MQ=255)
                                                                                                aaTATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATcaccg                                               >  7:354778/1‑149 (MQ=255)
                                                                                                        tCAGAAGCTGTTGAAGGATATTCCGTGGCAGGTTTGTACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCAGACTGCAGATCAGAGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCTCCagatgc                                       >  7:22885/1‑148 (MQ=255)
                                                                                                           gAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGAcc                                     >  1:207248/1‑148 (MQ=255)
                                                                                                           gAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGAcc                                     >  5:238964/1‑148 (MQ=255)
                                                                                                                 gTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAg                              >  6:238391/1‑149 (MQ=255)
                                                                                                                    gAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTAt                           >  3:280368/1‑149 (MQ=255)
                                                                                                                    gAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTAt                           >  6:94842/1‑149 (MQ=255)
                                                                                                                    gAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTAt                           >  7:61470/1‑149 (MQ=255)
                                                                                                                    gAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTAt                           >  3:315466/1‑149 (MQ=255)
                                                                                                                     aaGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCa                                                                      >  4:57685/1‑105 (MQ=255)
                                                                                                                     aaGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCa                                                                      <  3:57685/105‑1 (MQ=255)
                                                                                                                     aaGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTAt                           <  3:156782/148‑1 (MQ=255)
                                                                                                                      tggATATTCCGGGGCATGTTTGTACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACTCAATAATGGCCTACGGCAGATCAGCGCTTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTa                         <  8:22885/148‑1 (MQ=255)
                                                                                                                      agtatatTCCGGGGCAGGTTTATACGCCGCCTCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTa                         <  3:233712/146‑1 (MQ=255)
                                                                                                                      aGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACCCGCTAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGttt                           >  1:303197/1‑145 (MQ=255)
                                                                                                                       ggATATTGCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAg                        >  1:310844/1‑149 (MQ=255)
                                                                                                                       ggATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTCTACCACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAg                        <  6:228814/149‑1 (MQ=255)
                                                                                                                            ttCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGATGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACg                   >  3:45673/1‑149 (MQ=255)
                                                                                                                            ttCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGCCCGAAAAAGTATTAGGGACg                   >  7:123086/1‑149 (MQ=255)
                                                                                                                              ccGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACgg                  <  5:94842/148‑1 (MQ=255)
                                                                                                                                       gTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGc         >  7:243888/1‑148 (MQ=255)
                                                                                                                                        tttATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa       <  8:243888/149‑1 (MQ=255)
                                                                                                                                        tttATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCa       <  8:61470/149‑1 (MQ=255)
                                                                                                                                         ttATACGCCGCCGCAGCTAGGGAACTATTATTATGAGTATAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGATCGTCTGGCATTAATTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAg      <  6:324171/149‑1 (MQ=255)
                                                                                                                                             aCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACCCGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCGGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTg  >  1:211164/1‑149 (MQ=255)
                                                                                                                                             aCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTg  >  1:56227/1‑149 (MQ=255)
                                                                                                                                             aCGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTg  >  2:206419/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CTGCAAAAAGTGACCTTCCTGCCAATTAATCAGGAATCCGCAGCCACTAAGCGTTACCTCGCGGGGGATATTGATATCACCGAATCCTTCCCGAAAAATATGTATCAGAAGCTGTTGAAGGATATTCCGGGGCAGGTTTATACGCCGCCGCAGCTCGGGACCTATTATTATGCGTTTAACACGCAAAAAGGGCCGACGGCAGATCAGCGCGTTCGTCTGGCATTAAGTATGACGATAGATCGCCGCCTGATGACCGAAAAAGTATTAGGGACGGGCGAAAAGCCAGCGTG  >  NC_000913/1393941‑1394230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: