Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,455,598 C→T 5.3% G145G (GGC→GGT)  paaC → ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,455,5980CT 5.3% 242.8 / 2.4 75G145G (GGC→GGT) paaCring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand):  ref base C (38/33);  new base T (2/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

GTATTTCATCGATGCATGGCATGTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCG  >  NC_000913/1455454‑1455745
                                                                                                                                                |                                                                                                                                                   
gTATTTCATCGATGCATGTCATGTGGCGCTCTTTACGCGTCTGATGGAAAGCGGTGATCCGCAAGTGGCGGCGATTTCTGCCAATGCATTTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCGGGAGCGACTGGGCAATg                                                                                                                                                 <  4:143781/149‑1 (MQ=255)
      cATCGATGCATGGCATGTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACt                                                                                                                                            <  8:6383/148‑1 (MQ=255)
      cATCGATGCATGGCATGTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACt                                                                                                                                            >  2:362061/1‑148 (MQ=255)
       ctCGATGCATGGCATGTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGa                                                                                                                                          >  5:246327/2‑149 (MQ=255)
              catggcatgTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCa                                                                                                                                   <  4:332933/149‑1 (MQ=255)
               atggcatgTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGTAGCGACTGGGCAATGGTACTGACGTATCAg                                                                                                                                  >  1:298520/1‑149 (MQ=255)
                tggcatgTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAgg                                                                                                                                 <  6:298263/149‑1 (MQ=255)
                     tgtgGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCaa                                                                                                                             >  5:306577/1‑148 (MQ=255)
                     tgtgGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCaa                                                                                                                             >  4:17481/1‑148 (MQ=255)
                       tgGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCaaaa                                                                                                                           <  6:170721/148‑1 (MQ=255)
                       tgGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCaaaa                                                                                                                           <  5:80072/148‑1 (MQ=255)
                         gcgcTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGa                                                                                                                         <  6:246327/148‑1 (MQ=255)
                          cgcTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGAGTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATg                                                                                                                       <  3:133123/149‑1 (MQ=255)
                               tttACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATgcag                                                                                                                    >  1:340597/1‑147 (MQ=255)
                                 tACCCGGCTGATGGACAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAACCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGGGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGcagcag                                                                                                                 <  8:382654/148‑1 (MQ=255)
                                  aCCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGc                                                                                                               >  7:86453/1‑149 (MQ=255)
                                   cccGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGcc                                                                                                              <  3:17481/149‑1 (MQ=255)
                                   cccGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGcc                                                                                                              <  4:309877/149‑1 (MQ=255)
                                    ccGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATg                                                                                                                                                 <  8:249667/113‑1 (MQ=255)
                                    ccGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATg                                                                                                                                                 >  7:249667/1‑113 (MQ=255)
                                      ggcTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATc                                                                                                           <  1:62858/147‑1 (MQ=255)
                                         tGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGTAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATcaa                                                                                                         >  3:277616/1‑148 (MQ=255)
                                         tGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGTAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATcaa                                                                                                         >  3:282326/1‑148 (MQ=255)
                                           aTGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATcaac                                                                                                        >  1:53068/1‑147 (MQ=255)
                                           aTGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCGGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATcaac                                                                                                        >  5:242230/1‑147 (MQ=255)
                                               aaaGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGCCTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACACGTtg                                                                                                  >  6:177406/1‑149 (MQ=255)
                                                   ccGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGc                                                                                              >  5:22304/1‑149 (MQ=255)
                                                   ccGTGATCCGCAACTGGCGGCGATTTCTCCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGGCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTGGTGGc                                                                                              >  1:298768/1‑149 (MQ=255)
                                                      tGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGtt                                                                                           <  2:130443/149‑1 (MQ=255)
                                                             cAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTAccgc                                                                                    >  4:257071/1‑149 (MQ=255)
                                                                   gcggcgATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGaa                                                                                <  5:293493/147‑1 (MQ=255)
                                                                      gcgATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGtt                                                                           >  2:36978/1‑149 (MQ=255)
                                                                        gATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGAGTTTTAGTCGTGGCTGGCTGGAGCTACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTc                                                                          >  8:316472/1‑148 (MQ=255)
                                                                              tGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATgc                                                                                                                      <  4:318775/98‑1 (MQ=255)
                                                                              tGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATgc                                                                                                                      >  3:318775/1‑98 (MQ=255)
                                                                              tGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATg                                                                      >  8:310589/1‑146 (MQ=255)
                                                                              tGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATg                                                                      <  7:310589/146‑1 (MQ=255)
                                                                                ccAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCcg                                                                   <  6:121790/147‑1 (MQ=255)
                                                                                ccAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCcg                                                                   <  3:257071/147‑1 (MQ=255)
                                                                                       aaTTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGAtt                                                           >  8:319404/1‑148 (MQ=255)
                                                                                        aTTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGa                                                         >  3:36979/1‑149 (MQ=255)
                                                                                        aTTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGa                                                         <  6:22304/149‑1 (MQ=255)
                                                                                        aTTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGa                                                         >  4:131163/1‑149 (MQ=255)
                                                                                           aaaGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGatat                                                      >  3:340437/1‑149 (MQ=255)
                                                                                             agaagCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTg                                                    >  6:329702/1‑149 (MQ=255)
                                                                                                 gcgcgcTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGTAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACt                                                <  4:277616/149‑1 (MQ=255)
                                                                                                 gcgcgcTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGTAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACt                                                <  4:282326/149‑1 (MQ=255)
                                                                                                    cgcTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGtt                                                                           <  1:5367/119‑1 (MQ=255)
                                                                                                    cgcTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGtt                                                                           >  2:5367/1‑119 (MQ=255)
                                                                                                    cgcTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAg                                             >  1:34532/1‑149 (MQ=255)
                                                                                                         tCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAag                                        <  6:306577/149‑1 (MQ=255)
                                                                                                            ccTGCGTTTTAGTCGTGGGTGGCTGGAGCGACTGGGCATTGGTACTGACGTATCAGGGCAAAAGATGCTGCAGGCCATCAACTAGTTGTGGCGTTTTACAGCCGTACTGTTCGATGCCGAGGAGATTGATATTGCACTGAGTGAAGAgg                                     <  5:177406/149‑1 (MQ=255)
                                                                                                            ccTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAgg                                     <  7:368194/149‑1 (MQ=255)
                                                                                                             cTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGt                                            <  2:372260/141‑1 (MQ=255)
                                                                                                             cTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGt                                            >  1:372260/1‑141 (MQ=255)
                                                                                                              tGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAggg                                    >  5:233908/1‑148 (MQ=255)
                                                                                                                cGTTTTAGTCGTGGCTGGCTGGAGCTACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTa                                  <  5:140327/148‑1 (MQ=255)
                                                                                                                 gTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTAtt                                >  7:382681/1‑149 (MQ=255)
                                                                                                                   ttttGTCGTGGCTGGCAGGAGCGACAGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCTTTTTACCGCCGAACTGGTCGATGCCGACGAGATTGATAATGCACTGAGTGAAGAGGGTATTGc                              >  1:53363/1‑149 (MQ=255)
                                                                                                                      aGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCgg                            <  1:36978/148‑1 (MQ=255)
                                                                                                                      aGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGt                           >  7:122929/1‑149 (MQ=255)
                                                                                                                        tCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGtt                          >  2:139199/1‑148 (MQ=255)
                                                                                                                          gTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGa                        <  2:298520/148‑1 (MQ=255)
                                                                                                                               tggctggAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGtt                                                                                                   <  8:382047/68‑1 (MQ=255)
                                                                                                                               tggctggAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGtt                                                                                                   >  7:382047/1‑68 (MQ=255)
                                                                                                                                ggctggAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACg                  >  1:209228/1‑148 (MQ=255)
                                                                                                                                   tggAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGAGTGATATTGCACTGAGTGAAGAGGGTATTGCGGTGGATCCACGCACt              >  2:98681/1‑149 (MQ=255)
                                                                                                                                     gAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACtt             <  1:139199/148‑1 (MQ=255)
                                                                                                                                      aGCGACTGGGCAATGGTACTTACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGGTGTGGCGTTTTACCGCCGAACTGTACGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACttt            <  6:242230/148‑1 (MQ=255)
                                                                                                                                       gCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTAc          >  1:259503/1‑149 (MQ=255)
                                                                                                                                         gACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTAcgc        <  5:329702/149‑1 (MQ=255)
                                                                                                                                             gggCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAg    >  2:157212/1‑149 (MQ=255)
                                                                                                                                             gggCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAg    >  1:307489/1‑149 (MQ=255)
                                                                                                                                              ggCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGc   <  7:316472/149‑1 (MQ=255)
                                                                                                                                               gCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCg  >  7:325527/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GTATTTCATCGATGCATGGCATGTGGCGCTCTTTACCCGTCTGATGGAAAGCCGTGATCCGCAACTGGCGGCGATTTCTGCCAAAGCAATTAAAGAAGCGCGCTATCACCTGCGTTTTAGTCGTGGCTGGCTGGAGCGACTGGGCAATGGTACTGACGTATCAGGGCAAAAGATGCAGCAGGCCATCAACAAGTTGTGGCGTTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCG  >  NC_000913/1455454‑1455745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑