Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,885,952 G→A 5.5% A36A (GCG→GCA yeaX → putative YeaWX dioxygenase beta subunit, reductase component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,885,9520GA 5.5% 230.6 / 2.8 72A36A (GCG→GCAyeaXputative YeaWX dioxygenase beta subunit, reductase component
Reads supporting (aligned to +/- strand):  ref base G (30/38);  new base A (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTGGAGGAAAACTCGCGCGGCGGTTCC  >  NC_000913/1885804‑1886096
                                                                                                                                                    |                                                                                                                                                
gTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCg                                                                                                                                                  >  8:323230/1‑149 (MQ=255)
 tATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGt                                                                                                                                                 <  7:191530/149‑1 (MQ=255)
      tCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTaa                                                                                                                                             <  5:245354/148‑2 (MQ=255)
        cgccgcTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCg                                                                                                                                           <  4:146343/148‑1 (MQ=255)
             cTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAATCCATTACCTGCGTTTACCGGAGGaa                                                                                                                                     <  4:92066/149‑1 (MQ=255)
             cTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGaa                                                                                                                                     <  7:254126/149‑1 (MQ=255)
              tGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAg                                                                                                                                    >  5:185138/1‑149 (MQ=255)
                 gCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCa                                                                                                                                 >  6:49985/1‑149 (MQ=255)
                 gCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGGGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCa                                                                                                                                 >  8:284324/1‑149 (MQ=255)
                     aTTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGt                                                                                                                              <  4:41547/148‑1 (MQ=255)
                       ttttGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCAt                                                                                                                           >  6:219074/1‑149 (MQ=255)
                        tttGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCAtt                                                                                                                          >  2:17321/1‑149 (MQ=255)
                           gATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATtgtg                                                                                                                       >  3:340871/1‑149 (MQ=255)
                               gAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGa                                                                                                                   <  4:65119/149‑1 (MQ=255)
                                  tACAGCAATGTCAGACTATCAAATGTTTGAAGTACTGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGa                                                                                                                <  6:212074/149‑1 (MQ=255)
                                  tACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGa                                                                                                                <  5:160631/149‑1 (MQ=255)
                                   aCAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGa                                                                                                                <  4:84130/148‑1 (MQ=255)
                                    cAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGACCCCCTTACCGAACAGGTGAAACGCTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGc                                                                                                              <  2:65119/148‑1 (MQ=255)
                                       cAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACCGGGGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGAt                                                                                                           <  6:318774/149‑1 (MQ=255)
                                            tCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTg                                                                                                       <  1:17321/148‑1 (MQ=255)
                                             cAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGGTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGAt                                                                                                     <  5:111315/149‑1 (MQ=255)
                                                gcTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTTCAGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCATTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAAc                                                                                                  >  3:180590/2‑149 (MQ=255)
                                                aCTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAAc                                                                                                  <  7:207525/149‑1 (MQ=255)
                                                  tATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCa                                                                                                >  6:39652/1‑149 (MQ=255)
                                                  tATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCa                                                                                                <  5:219074/149‑1 (MQ=255)
                                                   aTCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAg                                                                                               >  7:316030/1‑149 (MQ=255)
                                                    tCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAg                                                                                               >  8:197127/1‑148 (MQ=255)
                                                    tCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAg                                                                                               <  5:49985/148‑1 (MQ=255)
                                                     cAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTa                                                                                             >  7:241326/1‑149 (MQ=255)
                                                      aaaTGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATg                                                                                                          >  6:337430/1‑135 (MQ=255)
                                                      aaaTGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATg                                                                                                          <  5:337430/135‑1 (MQ=255)
                                                       aaTGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACa                                                                                           <  2:171568/149‑1 (MQ=255)
                                                        aTGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATgg                                                                                                         >  7:4800/1‑134 (MQ=255)
                                                        aTGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATgg                                                                                                         <  8:4800/134‑1 (MQ=255)
                                                        aTGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAg                                                                                          >  3:210437/1‑149 (MQ=255)
                                                          gTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGc                                                                                         >  6:193417/1‑148 (MQ=255)
                                                               aaGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGc                                                                                    <  6:14080/148‑1 (MQ=255)
                                                                  tACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATTGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTAt                                                                                <  4:210437/149‑1 (MQ=255)
                                                                  tACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGc                                                                                         <  5:25211/140‑1 (MQ=255)
                                                                  tACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGc                                                                                         >  6:25211/1‑140 (MQ=255)
                                                                    cAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTc                                                                              <  6:30741/149‑1 (MQ=255)
                                                                     aGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCa                                                                             >  7:234851/1‑149 (MQ=255)
                                                                     aGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCa                                                                             <  7:197127/149‑1 (MQ=255)
                                                                        tGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCacta                                                                          <  7:323230/149‑1 (MQ=255)
                                                                            ccAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCactact                                                                        >  8:210962/1‑147 (MQ=255)
                                                                            ccAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCactact                                                                        >  6:105843/1‑147 (MQ=255)
                                                                              aGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCATTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGt                                                                    >  2:336293/1‑149 (MQ=255)
                                                                                    taCCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTcgccg                                                              <  5:39652/148‑1 (MQ=255)
                                                                                      ccccTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATtgtg                                                                                                                       <  8:232885/90‑1 (MQ=255)
                                                                                      ccccTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATtgtg                                                                                                                       >  7:232885/1‑90 (MQ=255)
                                                                                       cccTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCACCCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTcgccgctt                                                           >  6:80121/1‑147 (MQ=255)
                                                                                         cTTAACGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGa                                                         <  1:165501/149‑1 (MQ=255)
                                                                                              cGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACct                                                    >  4:2497/1‑149 (MQ=255)
                                                                                                aaCAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACctct                                                  <  3:2497/149‑1 (MQ=255)
                                                                                                 aCAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCtt                                                 <  7:284324/149‑1 (MQ=255)
                                                                                                  cAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCATTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACGAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCtt                                                 <  1:336293/148‑1 (MQ=255)
                                                                                                   aGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACCACTGAGTTCGCCGCATGACACCTCTTGt                                               <  6:185138/149‑1 (MQ=255)
                                                                                                     gTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTa                                             >  8:162087/1‑149 (MQ=255)
                                                                                                     gTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTa                                             >  8:157446/1‑149 (MQ=255)
                                                                                                        aaaCGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATc                                           <  5:193417/148‑1 (MQ=255)
                                                                                                            gCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGGCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGAtt                                      <  5:105843/149‑1 (MQ=255)
                                                                                                              ttCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGc                                    >  1:244649/1‑149 (MQ=255)
                                                                                                                cACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCg                                  >  8:268203/1‑149 (MQ=255)
                                                                                                                cACGCTGGTGGCAAACGATGGCAAACCATTACCTGCATTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATCACCAGTACAGCAATGCGTATTCACTACTGCGTTCGCCGCATGACACCTCTTGTTATCAGATTGcc                                   <  4:180590/148‑1 (MQ=255)
                                                                                                                       gtggCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCt                           <  7:268203/149‑1 (MQ=255)
                                                                                                                          gCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTgga                        <  7:162087/149‑1 (MQ=255)
                                                                                                                          gCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTg                          >  1:178982/1‑147 (MQ=255)
                                                                                                                                    gCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTGGAGGAAAACTcg              >  1:341897/1‑149 (MQ=255)
                                                                                                                                     cAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTAGAGGAAAACTcgc             >  6:288187/1‑149 (MQ=255)
                                                                                                                                        aCCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTGGAGGAAAACTcgcgcg          >  8:306664/1‑149 (MQ=255)
                                                                                                                                               ccTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTc                                                                              <  7:329053/74‑1 (MQ=255)
                                                                                                                                               ccTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTc                                                                              >  8:329053/1‑74 (MQ=255)
                                                                                                                                                cTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTGGAGGAAAACTCGCGCGGCGGTTcc  <  2:341897/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GTATTTTCCGCCGCTGGGCTGATTTTTGATGGAGTACAGCAATGTCAGACTATCAAATGTTTGAAGTACAGGTGAGCCAGGTTGAACCCCTTACCGAACAGGTGAAACGCTTCACGCTGGTGGCAACCGATGGCAAACCATTACCTGCGTTTACCGGAGGAAGTCACGTCATTGTGCAGATGAGCGATGGTGATAACCAGTACAGCAATGCGTATTCACTACTGAGTTCGCCGCATGACACCTCTTGTTATCAGATTGCCGTTCGGCTGGAGGAAAACTCGCGCGGCGGTTCC  >  NC_000913/1885804‑1886096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: