Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 347,188 G→A 5.7% A419V (GCG→GTG)  prpR ← propionate catabolism operon regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913347,1880GA 5.7% 278.7 / 2.2 86A419V (GCG→GTG) prpRpropionate catabolism operon regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (47/34);  new base A (2/3);  total (49/37)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGA  >  NC_000913/347047‑347334
                                                                                                                                             |                                                                                                                                                  
ggTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCa                                                                                                                                              <  7:18587/148‑1 (MQ=255)
   tCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAgaga                                                                                                                                          >  7:122336/1‑149 (MQ=255)
      acacTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGacac                                                                                                                                       >  1:3790/1‑149 (MQ=255)
       cacTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGCGCGGAGAGCGCCGCCAGAGACACt                                                                                                                                      <  7:319559/149‑1 (MQ=255)
       cacTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACACCTGCCGTAATGCAGCAGAAAATGGGGCGGAGAGCACCGCCAGAGACACt                                                                                                                                      >  5:176433/1‑149 (MQ=255)
        acTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCAGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGACACACtt                                                                                                                                     >  7:7885/1‑149 (MQ=255)
          ttAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACttt                                                                                                                                    >  5:360554/1‑148 (MQ=255)
            aaaaaCAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCa                                                                                                                                  >  5:268948/1‑148 (MQ=255)
               accAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCaaaaa                                                                                                                              <  4:6902/147‑1 (MQ=255)
               aaCAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCACCGCCAGAGACACTTTCaaaaa                                                                                                                              <  8:72602/149‑1 (MQ=255)
               aaCAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGACGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCaaa                                                                                                                                >  6:209724/1‑147 (MQ=255)
                      ccAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTcc                                                                                                                       >  5:11510/1‑149 (MQ=255)
                            gTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGc                                                                                                                 >  4:311783/1‑149 (MQ=255)
                            gTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCACTGGCGTAATGCAGCAGAAAATGGCGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGc                                                                                                                 >  5:323865/1‑149 (MQ=255)
                                catcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCgg                                                                                                               >  8:101683/1‑147 (MQ=255)
                                catcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGACGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGgaa                                                                                                             <  5:209724/149‑1 (MQ=255)
                                 atcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCgg                                                                                                               >  8:293504/1‑146 (MQ=255)
                                 atcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCgg                                                                                                               >  7:259448/1‑146 (MQ=255)
                                 atcatAATGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCgg                                                                                                               >  3:79163/1‑146 (MQ=255)
                                  tcatATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGgaaga                                                                                                           <  7:101683/149‑1 (MQ=255)
                                     tatTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAata                                                                                                        >  6:194687/1‑149 (MQ=255)
                                     tatTGCGCAGTTAACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGGCAGCGGAAGAata                                                                                                        >  2:213164/1‑149 (MQ=255)
                                         gcgcAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATcc                                                                                                     <  8:259448/148‑1 (MQ=255)
                                                  aCGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGCCACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATAACCGCCACCCGcg                                                                                           >  2:312640/1‑148 (MQ=255)
                                                  aCGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCAccactg                                                                                           >  1:293152/1‑145 (MQ=255)
                                                  aCGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCt                                                                                           >  3:242833/1‑149 (MQ=255)
                                                     aaTATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTcgc                                                                                        <  5:115738/149‑1 (MQ=255)
                                                       tatTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTcgcg                                                                                       >  4:1507/1‑148 (MQ=255)
                                                             cTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAgtg                                                                                  >  3:58221/1‑147 (MQ=255)
                                                              tGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAgtggtg                                                                               >  6:214784/1‑149 (MQ=255)
                                                                gCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAgtggtg                                                                               <  7:293504/147‑1 (MQ=255)
                                                                 ccAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGc                                                                                                                            >  8:77203/1‑101 (MQ=255)
                                                                       gTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTg                                                                        >  3:224578/1‑147 (MQ=255)
                                                                       gTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGc                                                                       <  3:311783/148‑1 (MQ=255)
                                                                         aGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGa                                                                    >  1:33343/1‑149 (MQ=255)
                                                                          gTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGAc                                                                   <  2:33343/149‑1 (MQ=255)
                                                                          gTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGAc                                                                   <  6:357527/149‑1 (MQ=255)
                                                                             cagcagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGc                                                                 >  2:32126/1‑148 (MQ=255)
                                                                                cagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCTCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCaaaa                                                             <  6:360554/149‑1 (MQ=255)
                                                                                cagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCACCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGc                                                                                                                 <  7:380819/97‑1 (MQ=255)
                                                                                cagcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCACCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGc                                                                                                                 >  8:380819/1‑97 (MQ=255)
                                                                                 agcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCGACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAAt                                                            <  4:79163/149‑1 (MQ=255)
                                                                                  gcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATa                                                           >  8:296001/1‑149 (MQ=255)
                                                                                  gcaCAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCACCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATa                                                           <  3:323532/149‑1 (MQ=255)
                                                                                   cacaGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAACTGGGGCGGAGAGCGCCGCCAGAGACACGTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGCCGCAAAATAc                                                          >  8:175925/1‑149 (MQ=255)
                                                                                     caGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCTGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTc                                                        <  1:312640/149‑1 (MQ=255)
                                                                                      aGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACt                                                         >  4:232920/1‑147 (MQ=255)
                                                                                      aGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCa                                                       <  5:214784/149‑1 (MQ=255)
                                                                                       gTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAg                                                      >  1:116113/1‑149 (MQ=255)
                                                                                          tCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGcc                                                    >  8:249753/1‑148 (MQ=255)
                                                                                            aCTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAGAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGa                                                  >  1:278969/1‑148 (MQ=255)
                                                                                            aCTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTc                                                                                          <  3:238922/108‑1 (MQ=255)
                                                                                            aCTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTc                                                                                          >  4:238922/1‑108 (MQ=255)
                                                                                            aCTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGa                                                  >  7:68579/1‑148 (MQ=255)
                                                                                            aCTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGAt                                                 <  4:242833/149‑1 (MQ=255)
                                                                                             cTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGAt                                                 >  3:21000/1‑148 (MQ=255)
                                                                                              ttGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCTCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATaa                                               <  3:232920/149‑1 (MQ=255)
                                                                                              ttGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATAc                                                          <  2:39474/138‑1 (MQ=255)
                                                                                              ttGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATAc                                                          >  1:39474/1‑138 (MQ=255)
                                                                                              ttGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCCGCCGATa                                                >  6:186773/1‑148 (MQ=255)
                                                                                                   tGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATaaaa                                             >  5:304913/1‑146 (MQ=255)
                                                                                                    gTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATaaaa                                             >  8:30269/1‑145 (MQ=255)
                                                                                                     tAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCACCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAg                                          <  6:176433/147‑1 (MQ=255)
                                                                                                       aCCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATc                                       <  4:178785/148‑1 (MQ=255)
                                                                                                       aCCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATc                                       <  1:213164/148‑1 (MQ=255)
                                                                                                       aCCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCa                                      <  7:296001/149‑1 (MQ=255)
                                                                                                         cccTGGCGTAATGCAGCAGAAAATGGGGCTGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACg                                    <  8:7885/149‑1 (MQ=255)
                                                                                                           cTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCTCAGTGGTGGCAATTGCAGACGCAAAATACTCCGCCGATAAAACAGATCACGGc                                  >  1:206066/1‑149 (MQ=255)
                                                                                                            tGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGc                                  >  5:148023/1‑148 (MQ=255)
                                                                                                                gTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGaaaa                             >  5:81518/1‑149 (MQ=255)
                                                                                                                 tAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAAc                            <  6:268948/149‑1 (MQ=255)
                                                                                                                 tAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAAc                            >  4:341704/1‑149 (MQ=255)
                                                                                                                    tGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTc                         >  4:111737/1‑149 (MQ=255)
                                                                                                                      cagcagAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCt                       >  5:87032/1‑149 (MQ=255)
                                                                                                                        gcagAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTtg                     <  6:81518/149‑1 (MQ=255)
                                                                                                                        gcagAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTtg                     >  2:230952/1‑149 (MQ=255)
                                                                                                                        gcagAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTtg                     <  8:122336/149‑1 (MQ=255)
                                                                                                                            aaaaTGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTtgctgc                 <  8:68579/149‑1 (MQ=255)
                                                                                                                              aaTGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCat               <  4:58221/149‑1 (MQ=255)
                                                                                                                                     cGGAGAGCGCCGCCAGAGACACTTTCAAACAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATAtcttct        <  2:3790/149‑1 (MQ=255)
                                                                                                                                     cGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTtgctg                  <  6:339749/139‑1 (MQ=255)
                                                                                                                                     cGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTtgctg                  >  5:339749/1‑139 (MQ=255)
                                                                                                                                     cGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATAtcttct        <  6:148023/149‑1 (MQ=255)
                                                                                                                                       gagaGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCttcttc      <  4:224578/149‑1 (MQ=255)
                                                                                                                                       gagaGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCttcttc      <  3:111737/149‑1 (MQ=255)
                                                                                                                                         gagCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCttcttc      >  5:138428/1‑147 (MQ=255)
                                                                                                                                           gcgcCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGa  <  6:11510/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
GGTTCCACACTTAAAAACAGCGCCAGTCGTTCCATCATATTGCGCAGTTCACGAATATTGCCTGGCCAGTCGTAGTGCAGCAGCACAGTTTCACTTGCCTGTAACCCCTGGCGTAATGCAGCAGAAAATGGGGCGGAGAGCGCCGCCAGAGACACTTTCAAAAAGCTTTCCGCCAGCGGAAGAATATCCGCCACCCGCTCGCGCAGTGGTGGCAATTGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGA  >  NC_000913/347047‑347334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑