BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,106,112	G→A	5.6%	T372I (ACT→ATT)	wbbH ←	O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,112	0	G	A	5.6%	346.9 / 6.3	108	T372I (ACT→ATT)	wbbH	O‑antigen polymerase
Reads supporting (aligned to +/- strand): ref base G (52/49); new base A (2/4); total (54/54)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAA  >  NC_000913/2105966‑2106259

    cATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAttt                                                                                                                                               >  4:160259/1‑149 (MQ=255)

    cATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAtt                                                                                                                                                <  2:94242/148‑1 (MQ=255)

     aTAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTg                                                                                                                                              >  6:2096/1‑149 (MQ=255)

        aTCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTa                                                                                                                                            <  8:239974/148‑1 (MQ=255)

          cTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATc                                                                                                                                          >  6:45840/1‑148 (MQ=255)

             aaGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAATAACTCTTTGTATcatag                                                                                                                                      <  1:229927/149‑5 (MQ=255)

             aaGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATc                                                                                                                                          <  5:5598/145‑1 (MQ=255)

             aaGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAAc                                                                                                                                      >  2:229714/1‑149 (MQ=255)

                 aaTGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCt                                                                                                                                  <  8:38772/149‑1 (MQ=255)

                   tGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTaa                                                                                                                                <  7:113414/149‑1 (MQ=255)

                        aGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTa                                                                                                                           <  1:280532/149‑1 (MQ=255)

                        aGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTa                                                                                                                           <  7:326487/149‑1 (MQ=255)

                          tGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAttt                                                                                                                                               >  8:1204/1‑127 (MQ=255)

                          tGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTAttt                                                                                                                                               <  7:1204/127‑1 (MQ=255)

                          tGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGt                                                                                                                         >  1:10899/1‑149 (MQ=255)

                            cATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTc                                                                                                                        <  8:158055/148‑1 (MQ=255)

                            cATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTc                                                                                                                        <  3:271508/148‑1 (MQ=255)

                             aTCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCAt                                                                                                                      >  5:205053/1‑149 (MQ=255)

                                ttttCTTGCTTTAAATCCAGCATCGCGGCTAGAGCAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACGATGATACAAAGCGTTATTTGTATCCAACTGCTAAGATTAGTCATg                                                                                                                     >  6:64999/1‑147 (MQ=255)

                                  ttCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGaa                                                                                                                   <  4:301157/147‑1 (MQ=255)

                                     ttGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCttt                                                                                                              >  5:283652/1‑149 (MQ=255)

                                      tGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCttt                                                                                                              >  7:350133/1‑148 (MQ=255)

                                         tttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAata                                                                                                                              <  5:235807/129‑1 (MQ=255)

                                         tttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAata                                                                                                                              >  6:235807/1‑129 (MQ=255)

                                         tttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCAt                                                                                                           >  4:234756/1‑148 (MQ=255)

                                         tttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATA‑TAGTCATGAAGCTTTCATGa                                                                                                         >  3:209832/1‑149 (MQ=255)

                                          ttAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGa                                                                                                         >  7:39507/1‑149 (MQ=255)

                                          ctAAATCCCCCATCGCCTCTACAGAAATTTAAATCAATCAAACAATACATTTTCACTTTATTTTCTGGG‑CTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGAt                                                                                                        <  4:259675/148‑1 (MQ=255)

                                                 cAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATaaaaaa                                                                                                  >  7:79601/1‑149 (MQ=255)

                                                  aGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAAt                                                                                                 <  1:229714/149‑1 (MQ=255)

                                                  aGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAAt                                                                                                 <  8:309249/149‑1 (MQ=255)

                                                   gCATCGCGTCTAGAGAAATTTAAATCACTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAAt                                                                                                 >  8:267042/1‑148 (MQ=255)

                                                     aTCGCGTCTAGATAAATTTAAATCATTCAAAAAATACAATTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaa                                                                                              <  2:193006/149‑1 (MQ=255)

                                                      tCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaa                                                                                             <  6:31311/149‑1 (MQ=255)

                                                      tCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaa                                                                                             <  1:264332/149‑1 (MQ=255)

                                                        gcgTATAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGACAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaag                                                                                            <  6:187364/148‑1 (MQ=255)

                                                          gTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaagaaa                                                                                         <  6:205053/149‑1 (MQ=255)

                                                          gTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaagaa                                                                                          >  8:182684/1‑148 (MQ=255)

                                                          gTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaagaa                                                                                          >  4:221133/1‑148 (MQ=255)

                                                             tAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAgg                                                                                      >  1:13274/1‑149 (MQ=255)

                                                             tAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGATAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAgg                                                                                      >  7:293499/1‑149 (MQ=255)

                                                              agagAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAgg                                                                                      >  3:140151/1‑148 (MQ=255)

                                                              agagAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGATTTCATGATAAAAAATGAAAGAAAAgg                                                                                      >  3:134987/1‑148 (MQ=255)

                                                               gagaAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGTCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGta                                                                                    <  7:58820/149‑1 (MQ=255)

                                                               gagaAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTaa                                                                                                                                >  7:189992/1‑105 (MQ=255)

                                                               gagaAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAg                                                                                       <  3:160259/146‑1 (MQ=255)

                                                                agaAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGtat                                                                                   <  3:265168/149‑1 (MQ=255)

                                                                agaAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGta                                                                                    >  8:34488/1‑148 (MQ=255)

                                                                 gaAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGtat                                                                                   >  6:295877/1‑148 (MQ=255)

                                                                  aaaTTTAAATCTTTCAAAAATTACATTTTCACTTTATTTTCTGGTCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTc                                                                                                                        <  5:92020/110‑1 (MQ=255)

                                                                  aaaTATAAATCATTCAAAAAATCCATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCATCTGGTAAGATTAGTc                                                                                                                        >  6:92020/1‑110 (MQ=255)

                                                                   aaTTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTataa                                                                                 <  2:10899/148‑1 (MQ=255)

                                                                          aTCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTataaa                                                                                <  3:139480/142‑1 (MQ=255)

                                                                           tCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAatat                                                                        <  3:221133/149‑1 (MQ=255)

                                                                             aTTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGa                                                                      <  2:13274/149‑1 (MQ=255)

                                                                                  aaaaaTACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATaa                                                                  >  7:119272/1‑148 (MQ=255)

                                                                                  aaaaaTACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATaa                                                                  >  2:97732/1‑148 (MQ=255)

                                                                                   aaaaTACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGA‑AATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATaaaa                                                                >  4:216093/1‑148 (MQ=255)

                                                                                    aaaTACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATa                                                                   <  8:35540/145‑1 (MQ=255)

                                                                                    aaaTACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATa                                                                   >  7:35540/1‑145 (MQ=255)

                                                                                           ttttCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTca                                                        <  8:203919/149‑1 (MQ=255)

                                                                                              tCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTcaca                                                      <  3:146366/148‑1 (MQ=255)

                                                                                              tCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTca                                                        >  5:31350/1‑146 (MQ=255)

                                                                                              tCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTca                                                        >  3:85143/1‑146 (MQ=255)

                                                                                                    tATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATg                                                >  1:291753/1‑148 (MQ=255)

                                                                                                     aTTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATAATAGTTATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAACATATGAATAAAATATTTTAACAGATATGt                                               >  6:186892/1‑148 (MQ=255)

                                                                                                             tgCCTTAAGAATTTGAGAGAATACTATGATACAAAGAGTTATTTTTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTc                                         <  1:256755/145‑1 (MQ=255)

                                                                                                             ggCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCg                                        <  8:39507/147‑1 (MQ=255)

                                                                                                               ccTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCgaga                                     >  6:19512/1‑148 (MQ=255)

                                                                                                                cTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGAc                                    >  2:325797/1‑148 (MQ=255)

                                                                                                                    aGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGtat                                                                                   >  6:98173/1‑97 (MQ=255)

                                                                                                                    aGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGtat                                                                                   <  5:98173/97‑1 (MQ=255)

                                                                                                                     gAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTCTCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTCTAAATAAAATCTGAATAAAATATTTTCACAGATATGTAATTTCGAGACAAtctc                              <  5:133603/149‑1 (MQ=255)

                                                                                                                       aaTTTAGAGAATACAATGATACAAAGATTTATTTGTATCCAACTGCTAATATTACTAATGAAGCTTTCATGATAAAAAATGAAAGAAAATGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCa                            <  3:300632/149‑1 (MQ=255)

                                                                                                                       aaTTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCa                            <  7:182684/149‑1 (MQ=255)

                                                                                                                             gagaATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATaaaa                       >  8:165804/1‑148 (MQ=255)

                                                                                                                              agaATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAata                                                              >  4:271285/1‑108 (MQ=255)

                                                                                                                              agaATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAata                                                              <  3:271285/108‑1 (MQ=255)

                                                                                                                               gaATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAacac                    >  2:155357/1‑149 (MQ=255)

                                                                                                                                aaTACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACAcc                   >  8:10046/1‑149 (MQ=255)

                                                                                                                                 aTACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCt                  <  4:85143/149‑1 (MQ=255)

                                                                                                                                      aTGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGaaa              >  1:74500/1‑148 (MQ=255)

                                                                                                                                       tGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGaaa              <  7:165804/147‑1 (MQ=255)

                                                                                                                                       tGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAAt             <  7:34488/148‑1 (MQ=255)

                                                                                                                                        gATACAAATAACTCTTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATCTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATa            >  2:134029/1‑148 (MQ=255)

                                                                                                                                        gATACAAATAACTCTTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATCTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATa            >  2:139102/1‑148 (MQ=255)

                                                                                                                                        gATACAAATAACTCTTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATCTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATa            <  1:139102/148‑1 (MQ=255)

                                                                                                                                        gATACAAATAACTCTTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATCTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATa            <  1:134029/148‑1 (MQ=255)

                                                                                                                                         aTACAAAGAGTTTTTTTTATACAACTTCTAATATTATTTATGAAGCTTTCATGATTAAAAATGAAAGAAAATGTATAAATAAGATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATAc           <  5:186892/148‑1 (MQ=255)

                                                                                                                                         aTACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATAc           <  8:131027/148‑1 (MQ=255)

                                                                                                                                          tACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGa                >  3:137171/1‑142 (MQ=255)

                                                                                                                                          tACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGa                <  4:137171/142‑1 (MQ=255)

                                                                                                                                           aCAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAg         >  3:312609/1‑148 (MQ=255)

                                                                                                                                             aaaGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCa      >  5:254470/1‑149 (MQ=255)

                                                                                                                                              aaGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATgaaa                                                                                             <  4:98152/61‑1 (MQ=255)

                                                                                                                                              aaGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGcc       <  7:337549/147‑1 (MQ=255)

                                                                                                                                              aaGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGcc       >  8:337549/1‑147 (MQ=255)

                                                                                                                                              aaGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCAt     <  5:295877/149‑1 (MQ=255)

                                                                                                                                               agagTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATg    <  5:255512/149‑1 (MQ=255)

                                                                                                                                                 agTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGaa  <  3:41150/149‑1 (MQ=255)

TTTTCATAATCTGAAGCAATGTCCAGTGCATCTTTTCTTGCTTTAAATCCAGCATCGCGTCTAGAGAAATTTAAATCATTCAAAAAATACATTTTCACTTTATTTTCTGGGCCTTAAGAAATTGAGAGAATACTATGATACAAAGAGTTATTTGTATCCAACTGCTAATATTAGTCATGAAGCTTTCATGATAAAAAATGAAAGAAAAGGTATAAATAAAATATGAATAAAATATTTTCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAA  >  NC_000913/2105966‑2106259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`