Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,134,710 T→C 5.2% N315D (AAC→GAC)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,134,7100TC 5.2% 236.8 / 2.7 77N315D (AAC→GAC) wzccolanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (33/40);  new base C (2/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

CCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTC  >  NC_000913/2134563‑2134855
                                                                                                                                                   |                                                                                                                                                 
ccGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTc                                                                                                                                                  <  2:48866/149‑1 (MQ=255)
     actcgACAGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGAGTGCGGTACTCCGTGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCa                                                                                                                                              <  7:273485/145‑1 (MQ=255)
      cgcgACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCat                                                                                                                                             <  6:17860/148‑1 (MQ=255)
       gcgACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatc                                                                                                                                            >  3:171040/1‑148 (MQ=255)
          aCCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGGTTCTCCAGCAGCGTGCGGTACGCCGGGGGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCatcgaat                                                                                                                                        <  7:382725/149‑1 (MQ=255)
              ttAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAg                                                                                                                                    >  7:376402/1‑149 (MQ=255)
                 aGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCAc                                                                                                                                 <  2:272006/149‑1 (MQ=255)
                       gCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCttt                                                                                                                           <  4:171040/149‑1 (MQ=255)
                        cTTTCTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCtttt                                                                                                                          >  7:296954/1‑149 (MQ=255)
                         tttttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                                                                                         >  1:276931/1‑149 (MQ=255)
                           tttCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCt                                                                                                                       >  6:85701/1‑149 (MQ=255)
                           tttCGTCTTCCAGCGCCTGACGTATCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTg                                                                                                                         <  2:331033/147‑1 (MQ=255)
                               gTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCa                                                                                                                   <  8:376402/149‑1 (MQ=255)
                                     cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGTGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGc                                                                                                              <  1:346751/148‑1 (MQ=255)
                                     cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCgg                                                                                                               >  1:364677/1‑147 (MQ=255)
                                     cAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCa                                                                                                             <  2:91985/149‑1 (MQ=255)
                                        cgcCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGAt                                                                                                          <  1:141496/149‑1 (MQ=255)
                                           cTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                                                                                       >  5:352765/1‑149 (MQ=255)
                                            tGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTCCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                                                                                       >  8:9535/1‑148 (MQ=255)
                                            tGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTCCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                                                                                       >  2:51493/1‑148 (MQ=255)
                                              aCGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                                                                                       >  8:358743/1‑146 (MQ=255)
                                              aCGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCaa                                                                                                       >  6:134871/1‑146 (MQ=255)
                                                gTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGaa                                                                                                  >  6:170482/1‑149 (MQ=255)
                                                 tttCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAAt                                                                                                 >  5:171156/1‑149 (MQ=255)
                                                    ctccttcagcGTGCTGTACGCCGTGTGAACTTTTGTGTACAGATTGTAGTTTTCCGCCTATTTAAATGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCTAATCGAGCACAGCTTTTGCTTCCTGCGTCAGATCAACAGAATCt                                                                                               <  1:97890/142‑1 (MQ=255)
                                                     tcCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCtt                                                                                              >  4:144427/1‑148 (MQ=255)
                                                       cagcagCGTGCGGTACGCCGTGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGCTCAGTTCGTTCAAATCCGCATCGATGTTCAACATCGAATCGATCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTa                                                                                            <  1:96486/148‑1 (MQ=255)
                                                       cagcagCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCtt                                                                                              <  6:171156/146‑1 (MQ=255)
                                                       cagcagCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAt                                                                                           >  5:254808/1‑149 (MQ=255)
                                                        agcagcGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTAt                                                                                           >  8:103023/1‑148 (MQ=255)
                                                         gcagcGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATcc                                                                                         >  8:265857/1‑149 (MQ=255)
                                                                 cGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGaa                                                                                 <  7:144410/149‑1 (MQ=255)
                                                                 cGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGaa                                                                                 <  4:154177/149‑1 (MQ=255)
                                                                       gCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCAtt                                                                           >  6:355559/1‑149 (MQ=255)
                                                                       gCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCAtt                                                                           >  6:76674/1‑149 (MQ=255)
                                                                         cGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCa                                                                         <  2:276931/149‑1 (MQ=255)
                                                                             tGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgttt                                                                     <  6:352765/149‑1 (MQ=255)
                                                                                 cTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTCCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgttt                                                                 <  1:51493/149‑1 (MQ=255)
                                                                                 cTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTCCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAgtttgttt                                                                 <  7:9535/149‑1 (MQ=255)
                                                                                    tGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTc                                                               <  5:355559/148‑1 (MQ=255)
                                                                                    tGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTc                                                               <  5:76674/148‑1 (MQ=255)
                                                                                    tGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTc                                                               <  5:85701/148‑1 (MQ=255)
                                                                                     ggTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGTGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCg                                                              <  5:349722/148‑1 (MQ=255)
                                                                                     ggTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCg                                                              <  5:134871/148‑1 (MQ=255)
                                                                                        gtACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCAATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCaa                                                          >  7:170083/1‑149 (MQ=255)
                                                                                         tACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCaa                                                          >  1:339191/1‑148 (MQ=255)
                                                                                                 ggAGATTTCCGCCTCTTTAAAGGTGAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCaa                                                   <  6:171982/147‑1 (MQ=255)
                                                                                                 ggAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCaa                                                   <  7:103023/147‑1 (MQ=255)
                                                                                                 ggAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAg                                                  <  7:265857/148‑1 (MQ=255)
                                                                                                  gagaTTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCg                                                <  6:254808/149‑1 (MQ=255)
                                                                                                    gaTTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGc                                              >  8:31996/1‑149 (MQ=255)
                                                                                                     aTTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTAACAATCGAATCGAGAACCGCTTTTTATACCAGCGGCAGAACAACAGAATCTTAATCCTGACGTAAGGCATTCAGTTTGTTTTAGGAAACATCTAAGCGGc                                              >  8:94253/1‑148 (MQ=255)
                                                                                                      tttCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTa                                            >  6:90135/1‑149 (MQ=255)
                                                                                                       ttCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTAc                                           >  1:122519/1‑149 (MQ=255)
                                                                                                         ccGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACg                                          >  7:60643/1‑148 (MQ=255)
                                                                                                          cGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTa                                        <  2:339191/149‑1 (MQ=255)
                                                                                                               ctTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGc                                                 <  2:308801/135‑1 (MQ=255)
                                                                                                               ctTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGc                                                 >  1:308801/1‑135 (MQ=255)
                                                                                                                tttAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCg                                  >  5:165354/1‑149 (MQ=255)
                                                                                                                 ttAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCgg                                 >  2:129351/1‑149 (MQ=255)
                                                                                                                  tAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCgg                                 <  7:358743/148‑1 (MQ=255)
                                                                                                                   aaaGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACg                                          <  1:163190/138‑1 (MQ=255)
                                                                                                                   aaaGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACg                                          >  2:163190/1‑138 (MQ=255)
                                                                                                                      ggTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCgg                                                             >  3:81010/1‑116 (MQ=255)
                                                                                                                      ggTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCgg                                                             <  4:81010/116‑1 (MQ=255)
                                                                                                                      ggTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACt                            >  8:106912/1‑149 (MQ=255)
                                                                                                                       gTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGc                                                            <  6:68747/116‑1 (MQ=255)
                                                                                                                           gttcgttcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCTAAGCGGCTACGTACTTCCGGTAACTGTTgc                       <  7:94253/149‑1 (MQ=255)
                                                                                                                                ttcAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAg                  <  3:144427/149‑1 (MQ=255)
                                                                                                                                           aTCAATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGc       <  8:170083/149‑1 (MQ=255)
                                                                                                                                             cGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCtt     >  7:378964/1‑149 (MQ=255)
                                                                                                                                               aTGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCtttt   <  5:90135/149‑1 (MQ=255)
                                                                                                                                               aTGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCtttt   >  6:272864/1‑149 (MQ=255)
                                                                                                                                               aTGGTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCtttt   <  8:296954/149‑1 (MQ=255)
                                                                                                                                                tGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTc                                    >  4:182927/1‑115 (MQ=255)
                                                                                                                                                tGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTc                                    <  3:182927/115‑1 (MQ=255)
                                                                                                                                                tGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTc  <  5:272864/149‑1 (MQ=255)
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CCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAACAGAATCTTTATCCTGACGGAAGGCATTCAGTTTGTTTTCGGCAACATCCAAGCGGCTACGTACTTCCGGTAACTGTTGCGCGAGGAAGGCGAGGCTTTTC  >  NC_000913/2134563‑2134855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: