Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,310,327 A→T 5.3% F27I (TTC→ATC) ‡ ada ← fused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,310,3270AT 5.3% 258.0 / 3.4 82F27I (TTC→ATC) ‡adafused DNA‑binding transcriptional dual regulator/O6‑methylguanine‑DNA methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (34/43);  new base T (2/2);  total (37/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

TGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTT  >  NC_000913/2310184‑2310473
                                                                                                                                               |                                                                                                                                                  
tGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCATAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGc                                                                                                                                               >  2:38053/1‑149 (MQ=255)
tGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGc                                                                                                                                               >  2:48844/1‑149 (MQ=255)
  gCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCg                                                                                                                                             >  3:259082/1‑149 (MQ=255)
  gCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCg                                                                                                                                             >  1:9559/1‑149 (MQ=255)
  gCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCg                                                                                                                                             <  1:38053/149‑1 (MQ=255)
   cTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGt                                                                                                                                            <  8:93963/149‑1 (MQ=255)
      aCAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCgg                                                                                                                                         >  2:261876/1‑149 (MQ=255)
       cggcGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGGAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGGGGCAAGGCGGGCGGCGAAAGATGCCTGTGGTACGCAGGGCGAGATCGAGTTCGGCGTCGGc                                                                                                                                        >  6:167578/4‑149 (MQ=255)
        aaCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCa                                                                                                                                       >  5:1066/1‑149 (MQ=255)
        aaCGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCa                                                                                                                                       >  5:316748/1‑149 (MQ=255)
           gTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCGGGCATTTGCGTAGAAGGAGACGTCTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCTAAAACGAATTCGACGTCGGCATTc                                                                                                                                    <  5:167578/149‑1 (MQ=255)
               gCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGt                                                                                                                                >  6:78959/1‑149 (MQ=255)
                 aGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcg                                                                                                                              <  6:255704/149‑1 (MQ=255)
                  ggggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgc                                                                                                                             >  8:110308/1‑149 (MQ=255)
                   gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                            >  1:294039/1‑149 (MQ=255)
                   gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                            <  2:9559/149‑1 (MQ=255)
                   gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                            >  1:315138/1‑149 (MQ=255)
                   gggCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTcgcg                                                                                                                            <  2:315138/149‑1 (MQ=255)
                     gCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCggg                                                                                                                          <  2:292107/149‑1 (MQ=255)
                     gCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATGTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGAACGCACGGCGAAAACGAAATCGCCGTCGGCATTCGGGTCGCggg                                                                                                                          >  7:151417/1‑149 (MQ=255)
                      ggAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGTAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGAAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGc                                                                                                                         <  8:125604/148‑1 (MQ=255)
                            ccAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGAc                                                                                                                   <  5:82333/149‑1 (MQ=255)
                             cAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACa                                                                                                                  <  5:78959/149‑1 (MQ=255)
                             cAGCGGCGAGTGCCGCGCTGGCAGTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACa                                                                                                                  <  3:287661/149‑1 (MQ=255)
                                cggcgTGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTTGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGAt                                                                                                               <  5:272702/149‑1 (MQ=255)
                                cggcgAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGAt                                                                                                               <  2:294039/149‑1 (MQ=255)
                                      gTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAg                                                                                                         >  3:38720/1‑149 (MQ=255)
                                         ccTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGt                                                                                                      <  4:239085/149‑1 (MQ=255)
                                          cTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGtt                                                                                                     >  7:43533/1‑149 (MQ=255)
                                             gCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGAt                                                                                                  <  5:181052/149‑1 (MQ=255)
                                               tGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGCCAGATTGCCAGCGTTGATCg                                                                                                >  4:272802/1‑149 (MQ=255)
                                                 gCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTc                                                                                              <  8:151417/149‑1 (MQ=255)
                                                  cATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCa                                                                                             <  1:333951/149‑1 (MQ=255)
                                                  cATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCa                                                                                             <  8:109565/149‑1 (MQ=255)
                                                        cGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTaa                                                                                        <  7:100269/148‑1 (MQ=255)
                                                        cGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAg                                                                                       >  8:305378/1‑149 (MQ=255)
                                                         gTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAg                                                                                       >  2:360263/1‑148 (MQ=255)
                                                         gTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGc                                                                                      <  7:110308/149‑1 (MQ=255)
                                                          tAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCa                                                                                     <  1:261876/149‑1 (MQ=255)
                                                            gAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtg                                                                                   <  4:38720/149‑1 (MQ=255)
                                                             aaGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtgt                                                                                  <  1:34363/149‑1 (MQ=255)
                                                             aaGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCAGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCAtgt                                                                                  >  8:359379/1‑149 (MQ=255)
                                                               ggAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTgg                                                                                >  2:336906/1‑149 (MQ=255)
                                                                      ttttCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCtttttt                                                                         <  7:235576/149‑1 (MQ=255)
                                                                        ttCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTc                                                                        <  6:1066/148‑1 (MQ=255)
                                                                          cccGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCAt                                                                      <  4:259082/148‑1 (MQ=255)
                                                                            cGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                                   <  4:300118/149‑1 (MQ=255)
                                                                             gCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAACCGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                                   >  1:5126/1‑148 (MQ=255)
                                                                             gCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATc                                                                  <  1:4071/149‑1 (MQ=255)
                                                                              cAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAAt                                                                   >  6:254838/1‑147 (MQ=255)
                                                                                ttcccgatCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCa                                                                 <  1:35987/140‑1 (MQ=255)
                                                                                aaGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGc                                                               <  7:246451/149‑1 (MQ=255)
                                                                                  gCATGTCTTGCGCGGCCAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGACAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTc                                                             <  2:263622/149‑1 (MQ=255)
                                                                                           gcgcGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCa                                                                 >  2:35987/1‑136 (MQ=255)
                                                                                              cgGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                                 <  7:305378/149‑1 (MQ=255)
                                                                                              cgGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                                 <  1:335761/149‑1 (MQ=255)
                                                                                                gCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAATCATGTGGCTTTTTTCAAAATCAGCTCCCTGGTAAAGGATa                                               <  8:218275/149‑1 (MQ=255)
                                                                                                 cAAGACGGACGGCAAAAGATGCCTGTGCTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAg                                              >  1:360827/1‑149 (MQ=255)
                                                                                                       ggacggCATAAGATGCCTGTGGTACGCACGGCGAATACTTATTCGCCGTCGGCTTTCTGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCTTAATAAGCTCCCTGGTTAAGGATAGCCTTTa                                        <  1:115919/149‑1 (MQ=255)
                                                                                                       ggacggCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTa                                        <  1:336906/149‑1 (MQ=255)
                                                                                                             cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAg                                                   <  2:225555/132‑1 (MQ=255)
                                                                                                             cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAg                                                   >  1:225555/1‑132 (MQ=255)
                                                                                                             cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGc                                  >  2:170937/1‑149 (MQ=255)
                                                                                                             cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGc                                  >  6:161747/1‑149 (MQ=255)
                                                                                                             cAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATAGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGc                                  >  4:6767/1‑149 (MQ=255)
                                                                                                              aaaaGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                                 <  8:214224/149‑1 (MQ=255)
                                                                                                              aaaaGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                                 >  7:246609/1‑149 (MQ=255)
                                                                                                              aaaaGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGcc                                 <  1:48844/149‑1 (MQ=255)
                                                                                                                    tGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGTTCGCGGGCTAAGACAGATTGCCAGCGTTGATAGTCAGTTAAGCATGTGTCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTc                            <  6:316748/148‑1 (MQ=255)
                                                                                                                       cTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTc                            >  8:81127/1‑145 (MQ=255)
                                                                                                                       cTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTc                            <  7:81127/145‑1 (MQ=255)
                                                                                                                          tgGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTTGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATcc                     >  3:179118/1‑148 (MQ=255)
                                                                                                                          tgGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCa                     >  5:37234/1‑149 (MQ=255)
                                                                                                                             tACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACg                   >  4:293968/1‑148 (MQ=255)
                                                                                                                                cgaCGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                                 <  1:132760/113‑1 (MQ=255)
                                                                                                                                cgaCGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGa                                                 >  2:132760/3‑115 (MQ=255)
                                                                                                                                  aCGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCaaaaa             >  4:242145/1‑149 (MQ=255)
                                                                                                                                   cGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAAc            >  6:138713/1‑149 (MQ=255)
                                                                                                                                   cGGCGAATTCGTTTTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAAc            <  5:138713/149‑1 (MQ=255)
                                                                                                                                         aaaCGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTGACCATCACGCAAAAACcaacaa      >  7:125453/1‑149 (MQ=255)
                                                                                                                                           aCGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATc    <  7:359379/149‑1 (MQ=255)
                                                                                                                                             gAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCtt  <  2:29798/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TGGCTGACAACGTTTGCAGGGGCGAAAGCCAGCGGCGAGTGCCTCGCTGGCATTTGCGTAGAAGGAGACGTTTTCCCGCAAAGCATGTCTGGCGCGGCAAGACGGACGGCAAAAGATGCCTGTGGTACGCACGGCGAAAACGAATTCGCCGTCGGCATTCGGGTCGCGGGCTAAGACAGATTGCCAGCGTTGATCGTCAGTTAAGCATGTGGCTTTTTTCATAATCAGCTCCCTGGTTAAGGATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTT  >  NC_000913/2310184‑2310473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑