Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,647,024 C→T 5.9% R101H (CGC→CAC)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,0240CT 5.9% 212.0 / 2.9 69R101H (CGC→CAC) pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base C (36/28);  new base T (2/2);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01

ATGCCATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAG  >  NC_000913/2646876‑2647167
                                                                                                                                                    |                                                                                                                                               
aTGCCATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGCTCTTGCCATGCTgcgc                                                                                                                                                 >  6:144188/1‑149 (MQ=255)
    cATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTgcgcgcgc                                                                                                                                             <  1:48867/149‑1 (MQ=255)
         cAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCAcc                                                                                                                                         >  2:325771/1‑148 (MQ=255)
            ttGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGCTCTTGCCATGCTGCGTGCGCCACCgag                                                                                                                                      >  7:136177/1‑148 (MQ=255)
             tGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACTAGCAACCTGCATAGTGAGCTTGCTGCCACCGGAAATAACCCGACCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACAGAGaa                                                                                                                                    >  3:246694/1‑149 (MQ=255)
              gCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGaa                                                                                                                                    <  7:8649/148‑1 (MQ=255)
               cAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCGTCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATg                                                                                                                                  <  6:140500/149‑1 (MQ=255)
               cAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGATGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCCTGCTGCGCGCGCCACCGAGAATg                                                                                                                                  >  7:153604/1‑149 (MQ=255)
                  cgcgcgCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGACGATCAAGCAGACGAGCAACCTCCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGAt                                                                                                                               <  4:152681/149‑1 (MQ=255)
                    cgcgcCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGCGAATGGAtt                                                                                                                              >  1:323115/1‑148 (MQ=255)
                                ggCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGc                                                                                                                 >  8:234240/1‑149 (MQ=255)
                                     aTTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCa                                                                                                            <  6:293741/149‑1 (MQ=255)
                                      ttttGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAg                                                                                                           <  5:144188/149‑1 (MQ=255)
                                           ccgccACATGTTTTGGGGTGCGGATCAAGCCGACGAGCAACCTGCATCGGGCGCGGGCTGCCACCGGAAATAACCCGGCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAAcc                                                                                                       <  8:153604/148‑1 (MQ=255)
                                              ccAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGc                                                                                                    >  7:252404/1‑148 (MQ=255)
                                                   tGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATcat                                                                                              >  1:149453/1‑147 (MQ=255)
                                                   tGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCtt                                                                                              <  1:325771/149‑1 (MQ=255)
                                                         ggggTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGACCTTCATaa                                                                                         <  7:329676/148‑1 (MQ=255)
                                                          ggggGAGCATCAAGCAGACGAGCAACCAGCATAGTTAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGAAATGCTGCTCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAAtt                                                                                       <  4:246694/149‑1 (MQ=255)
                                                             tGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGAt                                                                                    <  2:149453/149‑1 (MQ=255)
                                                                 gATCAAGCAGCCGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGCTGCTTCCAGCACCAGCGATCTTCATAATTGATCAgc                                                                                >  5:98901/1‑149 (MQ=255)
                                                                  aTCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATGAgcg                                                                               >  5:59232/1‑149 (MQ=255)
                                                                     aaGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCtt                                                                            >  7:1498/1‑149 (MQ=255)
                                                                      aGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCtt                                                                            >  4:4667/1‑148 (MQ=255)
                                                                      aGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCtt                                                                            >  3:272001/1‑148 (MQ=255)
                                                                      aGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCtt                                                                            >  4:261013/1‑148 (MQ=255)
                                                                       gCAGCCGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACACCCGGATGCTTCCAGACCCATCGATATTCATAATTGAGCAGCGCTTc                                                                           >  2:141648/1‑148 (MQ=255)
                                                                        cAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCaa                                                                         <  1:372172/149‑1 (MQ=255)
                                                                             gAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTaa                                                                    >  5:234763/1‑149 (MQ=255)
                                                                               tcAACCTTCATAGTGAGCGTGCTGCCACCGGAAATAACGCTTCCCGAAGTGAGATATTTCCATGCTTCGCTTGACACAGAGAATGGATTCACCCCCGGATGCTTCCAGAAc                                                                                                        <  4:106194/110‑1 (MQ=255)
                                                                               gCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGatc                                                                                                        >  3:106194/1‑109 (MQ=255)
                                                                                cAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGt                                                                 <  4:280035/149‑1 (MQ=255)
                                                                                 aaCCTGCATAGTGAGCGTGCTGCCACCGGACATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTg                                                                <  8:71334/149‑1 (MQ=255)
                                                                                    cTGCATAGTGAGCGTGCTGCCACCGGAAATAACCAGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTg                                                                >  8:314948/1‑146 (MQ=255)
                                                                                      gCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGTGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATc                                                                                   <  2:284602/125‑1 (MQ=255)
                                                                                      gCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGTGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATc                                                                                   >  1:284602/1‑125 (MQ=255)
                                                                                         tAGAGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTAACCCCCGGATTCTTCCAGAACCAGACATCTTCATAAATGATCAGCGCTTCAAGGTAAAGTGGAGAAAc                                                         >  3:73158/1‑148 (MQ=255)
                                                                                            tGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCt                                                     <  3:4667/149‑1 (MQ=255)
                                                                                              aGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTc                                                   <  4:272001/149‑1 (MQ=255)
                                                                                               gCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTc                                                   >  7:360075/1‑148 (MQ=255)
                                                                                                 gTGCTGCCACCGGAAATCCCCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGAt                                                <  1:141648/149‑1 (MQ=255)
                                                                                                  tgctgcCACCGGAAATAAGCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCGACCGAGAATGGATTCGCCCCCGGATGCGTCCAGACCCAGCGATCTTCATAATTGGTCAGCGCTTCAAGGGAACGGTGAGAAACATCTTCGAtg                                               >  6:268063/1‑148 (MQ=255)
                                                                                                  ggctgcCACCGGAAATAACCCGTCCCGCAGTGAGATCTTGCCATGCTGCGTGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGAtt                                               <  8:136177/148‑1 (MQ=255)
                                                                                                   gctgcCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTg                                              >  4:89379/1‑149 (MQ=255)
                                                                                                    ctgcCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGt                                             >  3:303024/1‑149 (MQ=255)
                                                                                                         aCCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCg                                        >  8:195582/1‑149 (MQ=255)
                                                                                                         aCCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCg                                        >  1:46059/1‑149 (MQ=255)
                                                                                                          ccGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGAGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCg                                        >  1:19353/1‑148 (MQ=255)
                                                                                                            ggAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGAt                                     <  3:306193/149‑1 (MQ=255)
                                                                                                              aaaTAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATaa                                   <  3:151211/149‑1 (MQ=255)
                                                                                                                 tAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGc                                >  2:210147/1‑149 (MQ=255)
                                                                                                                    cccGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGc                                >  5:40413/1‑146 (MQ=255)
                                                                                                                    cccGTCCCGAAGTGAGATCTTGACATGCTGCGCTCGCCACCGAGAATGGATTCACCCCAGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAg                             <  2:267697/149‑1 (MQ=255)
                                                                                                                        tCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGc                         >  5:18122/1‑149 (MQ=255)
                                                                                                                          ccGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCg                       >  8:223526/1‑149 (MQ=255)
                                                                                                                           cGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGt                      <  1:44448/149‑1 (MQ=255)
                                                                                                                            gAAGTGAGATCTTGCCATGCTGCGGGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAg                             <  2:373963/141‑1 (MQ=255)
                                                                                                                            gAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGaagagcg                      >  1:373963/1‑142 (MQ=255)
                                                                                                                            gAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTc                     <  3:89379/149‑1 (MQ=255)
                                                                                                                                tGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTc                                                   >  7:121006/1‑115 (MQ=255)
                                                                                                                                tGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTc                                                   <  8:121006/115‑1 (MQ=255)
                                                                                                                                    aTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCg              <  2:46059/148‑1 (MQ=255)
                                                                                                                                         gCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGc            <  7:234240/145‑1 (MQ=255)
                                                                                                                                          ccATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTACATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAgc       <  2:323115/149‑1 (MQ=255)
                                                                                                                                           cATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAgcg      >  2:5608/1‑149 (MQ=255)
                                                                                                                                            aTGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAgcgc     <  8:1498/149‑1 (MQ=255)
                                                                                                                                             tgctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAgcgc     >  6:121201/1‑148 (MQ=255)
                                                                                                                                              gctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAgcgc     >  6:310729/1‑147 (MQ=255)
                                                                                                                                               ctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCag  >  1:157909/1‑149 (MQ=255)
                                                                                                                                               ctgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCa   <  7:314948/148‑1 (MQ=255)
                                                                                                                                                tgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCa   >  2:170723/1‑147 (MQ=255)
                                                                                                                                                  cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGcc    <  4:302797/144‑1 (MQ=255)
                                                                                                                                                  cgcgcgcCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGcc    >  3:302797/1‑144 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ATGCCATTCCAGTTGCAACGCGCGCCAGAGCTGGCGAATTTTGCCGCCAAATGTTTTGGGGTGAGGATCAAGCAGACGAGCAACCTGCATAGTGAGCGTGCTGCCACCGGAAATAACCCGTCCCGAAGTGAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAG  >  NC_000913/2646876‑2647167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: