Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,900,327 A→C 7.5% intergenic (‑54/‑265) ygcW ← / → yqcE putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,900,3270AC 7.5% 334.0 / 17.6 117intergenic (‑54/‑265)ygcW/yqcEputative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (47/61);  new base C (5/4);  total (52/65)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.63e-01

Only 100 of 117 total aligned reads displayed.

TAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAA  >  NC_000913/2900184‑2900473
                                                                                                                                               |                                                                                                                                                  
tAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAgg                                                                                                                                                >  8:366781/1‑148 (MQ=255)
 aaaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAggg                                                                                                                                               >  6:16831/1‑148 (MQ=255)
  aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGaa                                                                                                                                             <  5:188482/149‑1 (MQ=255)
     cGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGtt                                                                                                                                          <  7:147450/149‑1 (MQ=255)
      gCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTa                                                                                                                                         <  4:177532/149‑1 (MQ=255)
       cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTAc                                                                                                                                        <  4:122720/149‑1 (MQ=255)
         aTTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCt                                                                                                                                      >  4:112728/1‑149 (MQ=255)
         aTTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCt                                                                                                                                      <  8:310612/149‑1 (MQ=255)
          ttCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTATCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCACCCGCGAGAGGGAAGTTACAtt                                                                                                                                     >  5:287569/1‑149 (MQ=255)
          ttCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCAATTGAGAACGCATTGAGAGATTCGATTGAAATAGTTAGCTCCAATTTCAGTAAGGCAAAAAAAAACAAAGGTAAATTACCCCCCGAACGGGAAGTAACAtt                                                                                                                                     >  7:318797/1‑149 (MQ=255)
             ccACCGGTACCAATTGCGGTATTACGTTTCAGGCAGAAAAACTCCATCGAGATCGCATTGAGAGAGTCGCATGACATAGAGAGCTCCAATTGCCGTAATGCAAAAAAAAAGAAAGGTAACTTCCCCGGCGAGAGGGAAGTTACCTTtct                                                                                                                                  <  6:278517/149‑1 (MQ=255)
              cACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtctc                                                                                                                                 <  1:355582/149‑1 (MQ=255)
              cACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTtct                                                                                                                                  >  7:228098/1‑148 (MQ=255)
                ccGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCAt                                                                                                                               >  7:96511/1‑149 (MQ=255)
                 cGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                                                                                                       <  6:57647/140‑1 (MQ=255)
                 cGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTAcc                                                                                                                                       >  5:57647/1‑140 (MQ=255)
                   gTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAt                                                                                                                            >  4:67800/1‑149 (MQ=255)
                      aCAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTAtctc                                                                                                                         <  4:138351/149‑1 (MQ=255)
                        aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTg                                                                                                                       >  7:279670/1‑149 (MQ=255)
                        aaTTGCGGTTTTACCTTTCAGGGAGAAAAAAGCCGTTGGGAACGCATTGAGAGATGCGATTGACGTGGTTAGCGCCAATTTCCGTAAGGCAAAAAAAAAGAAGGGTCACTTCCCCCGCGCGAGGGAGGTTGCCGTGCTCATTGtctcgg                                                                                                                       >  4:264756/1‑147 (MQ=255)
                         aTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                      <  2:332784/149‑1 (MQ=255)
                         aTTGCGGTTCTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                      <  4:301873/149‑1 (MQ=255)
                             cGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAAc                                                                                                                  <  1:193121/149‑1 (MQ=255)
                                      cTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                      >  7:191371/1‑120 (MQ=38)
                                      cTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGAAGTTACCt                                                                                                                                      <  8:191371/120‑1 (MQ=38)
                                      cTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATaa                                                                                                         <  7:55352/149‑1 (MQ=255)
                                          cAGGGAGAAAAATTCCTTTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGAAAAAAAAAAGAAAGGTATCTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt                                                                                                      <  4:208793/148‑1 (MQ=255)
                                            gtgagaAAAAATACATTGATAAAGCATTGAGAGATTCGATTGACATAGTTAGCTCAAATTTCCGTAAGGCAAAAAAATAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                   <  6:287569/147‑1 (MQ=255)
                                            gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                   <  1:256563/149‑1 (MQ=255)
                                            gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                   <  5:17113/149‑1 (MQ=255)
                                            gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                   <  4:169667/149‑1 (MQ=255)
                                              gagaAAAACTCCATTGAGAACGGAATGAGAGATTCCATTGACATAGTTAGCTCCAATTTACGTAAGGCCAAAAAAAAGAAAGTTAACTTCCCCCGCTAGAGGCAAGTTACCTTTCTCATTATCTCTTGTAACTTTAGATAATTTTTAGc                                                                                                 <  6:127837/149‑1 (MQ=255)
                                               agaAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAgaa                                                                                                >  7:196227/1‑147 (MQ=255)
                                                   aaaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAg                                                                                            >  5:6503/1‑149 (MQ=255)
                                                    aaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg                                                                                           >  1:7380/1‑149 (MQ=255)
                                                     aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtttt                                                                                                     <  6:352889/138‑1 (MQ=255)
                                                     aaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtttt                                                                                                     >  5:352889/1‑138 (MQ=255)
                                                      aTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAggg                                                                                          >  7:282122/1‑148 (MQ=255)
                                                       tCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                  >  1:11951/1‑139 (MQ=255)
                                                       tCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                  <  2:11951/139‑1 (MQ=255)
                                                         cATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                                                                       >  7:179788/1‑148 (MQ=255)
                                                            tGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCGCCGCGAGAGGGATGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                     <  2:54196/147‑1 (MQ=255)
                                                            tGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACATTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACt                                                                                   >  5:34872/1‑149 (MQ=255)
                                                              agaACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTg                                                                                  <  3:67800/148‑1 (MQ=255)
                                                                aaCGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTa                                                                               <  2:120905/149‑1 (MQ=255)
                                                                   gCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtg                                                                            >  2:100988/1‑149 (MQ=255)
                                                                     aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGaa                                                                                                                                             >  8:237968/1‑82 (MQ=37)
                                                                     aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCTCTCGCGGGGGaa                                                                                                                                             <  7:237968/82‑1 (MQ=37)
                                                                     aTTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAtgt                                                                           >  8:351735/1‑148 (MQ=255)
                                                                      ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                                                                         >  2:323447/1‑149 (MQ=255)
                                                                      ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                                                                         >  2:150441/1‑149 (MQ=255)
                                                                      ttGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGa                                                                         <  4:86813/149‑1 (MQ=255)
                                                                         agagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                       <  4:19066/148‑1 (MQ=255)
                                                                             aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                       <  8:201941/144‑1 (MQ=255)
                                                                             aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                  >  2:361641/1‑149 (MQ=255)
                                                                              ttCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATc                                                                 <  6:279706/149‑1 (MQ=255)
                                                                               tCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATca                                                                >  1:271722/1‑149 (MQ=255)
                                                                                 gATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca                                                              <  7:157424/149‑1 (MQ=255)
                                                                                   ttGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt                                                            <  8:279670/149‑1 (MQ=255)
                                                                                      tcATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt                                                          <  8:318797/147‑1 (MQ=255)
                                                                                      aCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAATGGAATAACTGCTAATGTGATCGTAATCACAgtgt                                                          >  3:274887/1‑148 (MQ=255)
                                                                                      aCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                         <  1:83148/149‑1 (MQ=255)
                                                                                        aTAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGa                                                        >  4:60685/1‑148 (MQ=255)
                                                                                         tAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGTGCATAACt                                                                                   >  5:140853/1‑120 (MQ=255)
                                                                                         tAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACt                                                                                   <  6:140853/120‑1 (MQ=255)
                                                                                         tAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGata                                                      <  7:351735/149‑1 (MQ=255)
                                                                                            ttAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTc                                                   <  5:245351/149‑1 (MQ=255)
                                                                                               gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTa                                                <  8:153572/149‑1 (MQ=255)
                                                                                                 tCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                              >  5:153564/1‑149 (MQ=255)
                                                                                                 tCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                              >  7:161626/1‑149 (MQ=255)
                                                                                                   cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGt                                            >  1:272003/1‑149 (MQ=255)
                                                                                                      tttCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCaa                                         <  1:361641/149‑1 (MQ=255)
                                                                                                      tttCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCa                                          >  1:364916/1‑148 (MQ=255)
                                                                                                         ccGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaat                                       <  8:282122/148‑1 (MQ=255)
                                                                                                          cGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGGTATTCGTATTGTCGaataa                                     >  7:52376/1‑149 (MQ=255)
                                                                                                            tAAGGCAAAAAAAAAGATAGGTAACTTCCCCCGAGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                   <  1:58073/149‑1 (MQ=255)
                                                                                                            tAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                   <  5:16831/149‑1 (MQ=255)
                                                                                                            tAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                   >  4:167201/1‑149 (MQ=255)
                                                                                                                gCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTa                                >  6:20567/1‑148 (MQ=255)
                                                                                                                 cAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                              <  3:60685/149‑1 (MQ=255)
                                                                                                                  aaaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                              >  1:230788/1‑148 (MQ=255)
                                                                                                                   aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaaa                            <  5:20567/149‑1 (MQ=255)
                                                                                                                   aaaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaa                              >  2:328494/1‑147 (MQ=255)
                                                                                                                    aaaaaaaGAATGGTAACTTCCCCCGGGAGATGGAAGTTACCTTTCTAATTATCTCTGGTAACTGGAGATTATTTTTAGCATAAGTTCATAACTGCTAATGTGATCGTAATCTCAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                           <  4:274887/149‑1 (MQ=255)
                                                                                                                    aaaaaaaGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                           >  7:88042/1‑149 (MQ=255)
                                                                                                                         aagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGaa                      <  1:140866/149‑1 (MQ=255)
                                                                                                                         aagaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGaa                      <  5:202892/149‑1 (MQ=255)
                                                                                                                           gaaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGc                    <  8:228098/149‑1 (MQ=255)
                                                                                                                            aaagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCg                   <  8:88042/149‑1 (MQ=255)
                                                                                                                             aagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                      <  4:123587/49‑1 (MQ=255)
                                                                                                                             aagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTgg                                                                                                                      >  3:123587/1‑49 (MQ=255)
                                                                                                                             aagGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGt                  <  2:7380/149‑1 (MQ=255)
                                                                                                                                gTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCGTAAg                                                                                            <  4:23653/72‑1 (MQ=255)
                                                                                                                                gTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCGTAAg                                                                                            >  3:23653/1‑72 (MQ=255)
                                                                                                                                         cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTa             >  2:157124/1‑142 (MQ=255)
                                                                                                                                         cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTa             <  1:157124/142‑1 (MQ=255)
                                                                                                                                         cccGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTca       >  8:162046/1‑148 (MQ=255)
                                                                                                                                          ccGCGAGAGGGAAGTTACCTTTCTCATTCTCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcaca     <  2:96616/149‑1 (MQ=255)
                                                                                                                                           cgcgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACACTGTGatta                                                     <  6:122126/100‑3 (MQ=255)
                                                                                                                                             cgAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACacaa  <  1:328494/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
TAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGAGGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAA  >  NC_000913/2900184‑2900473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: