BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,936,203	C→T	5.1%	A207V (GCG→GTG)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,203	0	C	T	5.1%	316.2 / 3.1	96	A207V (GCG→GTG)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand): ref base C (44/47); new base T (2/3); total (46/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAATGCCGAGCA  >  NC_000913/2936055‑2936347

aaaCTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGc                                                                                                                                                  <  8:88811/149‑1 (MQ=255)

    tgctgcGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTg                                                                                                                                              >  3:274334/1‑149 (MQ=255)

      ctgcGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTg                                                                                                                                              <  8:349597/147‑1 (MQ=255)

      ctgcGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGa                                                                                                                                            <  5:33274/149‑1 (MQ=255)

       tgcGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTACTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGat                                                                                                                                           <  6:89843/149‑1 (MQ=255)

        gcgcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGTGGTGGata                                                                                                                                          >  8:153030/1‑149 (MQ=255)

        gcgcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGTGGTGGata                                                                                                                                          >  8:147915/1‑149 (MQ=255)

         cgcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGata                                                                                                                                          <  7:190122/148‑1 (MQ=255)

          gcTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGCTCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATg                                                                                                                                        >  5:281744/1‑149 (MQ=255)

              tGCCCGCGCCGGTTTGGCCGTCGCCAGGATGAAAGGTAAAATCTATCTGTCGCTGGGCGGCGTTTCGATGGGGATCTCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGAcg                                                                                                                                     <  5:70605/148‑2 (MQ=255)

                 ccGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAAc                                                                                                                                 >  6:180397/1‑149 (MQ=255)

                     gcCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCg                                                                                                                             >  5:135858/1‑149 (MQ=255)

                              gCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGTGGTGGATATGACCGAACTGCGTCGCCGTAt                                                                                                                    <  7:153030/149‑1 (MQ=255)

                              gCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGTGGTGGATATGACCGAACTGCGTCGCCGTAt                                                                                                                    <  7:147915/149‑1 (MQ=255)

                              gCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTa                                                                                                                     <  1:69437/148‑1 (MQ=255)

                               ccGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATc                                                                                                                   >  3:265963/1‑149 (MQ=255)

                                  tCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGAt                                                                                                                <  8:338387/149‑1 (MQ=255)

                                    gCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATc                                                                                                               <  4:59478/148‑1 (MQ=255)

                                     ccAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGAt                                                                                                                >  6:250332/1‑146 (MQ=255)

                                      gggCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGTGGTGGATATGACCGAACTGCGTCGCCGTATCGATCaga                                                                                                            <  4:142783/147‑1 (MQ=255)

                                           tGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAtt                                                                                                       >  4:298589/1‑149 (MQ=255)

                                           tGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAtt                                                                                                       >  8:211981/1‑149 (MQ=255)

                                            gAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAtt                                                                                                       >  2:132415/1‑148 (MQ=255)

                                            gAAAGGTAAAAGCTAGCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAtt                                                                                                       >  3:33976/1‑148 (MQ=255)

                                             aaaGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAttt                                                                                                      <  6:74994/148‑1 (MQ=255)

                                             aaaGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGAttt                                                                                                      <  7:174688/148‑1 (MQ=255)

                                             aaaGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTa                                                                                                     <  6:47424/149‑1 (MQ=255)

                                             aaaGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTa                                                                                                     <  7:211981/149‑1 (MQ=255)

                                             aaaGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTACCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTa                                                                                                     <  5:115925/149‑1 (MQ=255)

                                                 gTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTacgac                                                                                                 <  3:298589/149‑1 (MQ=255)

                                                  tAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTacgac                                                                                                 >  7:316133/1‑148 (MQ=255)

                                                    aaaGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGaa                                                                                              <  5:256995/149‑1 (MQ=255)

                                                    aaaGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGaa                                                                                              <  3:117608/149‑1 (MQ=255)

                                                     aaGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGCAg                                                                                             >  8:351912/1‑149 (MQ=255)

                                                      aGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTAGCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGc                                                                                            <  2:275394/149‑1 (MQ=255)

                                                          aTCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGaa                                                                                        >  3:285840/1‑149 (MQ=255)

                                                           tCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGaa                                                                                        <  8:37967/148‑1 (MQ=255)

                                                             tGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAAt                                                                                       >  7:294662/1‑147 (MQ=255)

                                                             tGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAAt                                                                                       <  8:294662/147‑1 (MQ=255)

                                                             tGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTg                                                                                     <  3:245258/149‑1 (MQ=255)

                                                               tCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAAtt                                                                                      <  7:337640/146‑1 (MQ=255)

                                                               tCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCGGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAAt                                                                                       <  4:265963/145‑1 (MQ=255)

                                                               tCGCTGGGCGACGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTgg                                                                                    <  2:149572/148‑1 (MQ=255)

                                                                  cTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAAt                                                                                <  4:274334/149‑1 (MQ=255)

                                                                    tggcggcGGTTCGAGCGGTCTCGCCGGTTCCATTGTTGCTCACAACTTCTTTGAATCCTGGCGGGGAATGAAAGTCCAGGCGGTTGATATGACCGAACTGCGTCGCCGTATAGATCAGAAGATTTACGACGCAGCCGAATTGGAAATgg                                                                              <  2:278348/148‑1 (MQ=255)

                                                                        ggcgTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGAc                                                                                                                                      <  7:312642/89‑1 (MQ=255)

                                                                        ggcgTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGAc                                                                                                                                      >  8:312642/1‑89 (MQ=255)

                                                                         gcgTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTg                                                                         <  6:281744/149‑1 (MQ=255)

                                                                            tttCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGcc                                                                      <  3:168512/149‑1 (MQ=255)

                                                                              tCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCt                                                                     <  5:285583/148‑1 (MQ=255)

                                                                               cGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCGGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGGCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTg                                                                    <  1:101835/148‑1 (MQ=255)

                                                                                gATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTggg                                                                  >  6:34552/1‑149 (MQ=255)

                                                                                gATGGGTATCGCCGGTTCCATTGTTCATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTggg                                                                  >  2:104884/1‑149 (MQ=255)

                                                                                     gTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGa                                                              >  1:166833/1‑148 (MQ=255)

                                                                                        tCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCt                                                                     <  3:266748/138‑1 (MQ=255)

                                                                                        tCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCt                                                                     >  4:266748/1‑138 (MQ=255)

                                                                                        tCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATaa                                                           >  4:122788/1‑148 (MQ=255)

                                                                                        tCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATaa                                                           >  8:245457/1‑148 (MQ=255)

                                                                                        tCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATaa                                                           >  5:32735/1‑148 (MQ=255)

                                                                                          gCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTggg                                                                  <  4:329118/139‑1 (MQ=255)

                                                                                          gCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTggg                                                                  >  3:329118/1‑139 (MQ=255)

                                                                                             ggTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACtt                                                     >  2:220640/1‑149 (MQ=255)

                                                                                                tCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCg                                                  >  1:216623/1‑149 (MQ=255)

                                                                                                   aTTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTa                                               >  5:85712/1‑149 (MQ=255)

                                                                                                   aTTGTTGATCACAACTTCTTTGAATCCTGGCGGGGAATGAAAGGCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTa                                               <  2:39239/149‑1 (MQ=255)

                                                                                                      gttgATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATg                                             <  5:34552/148‑1 (MQ=255)

                                                                                                          aTCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCg                                          >  8:365611/1‑147 (MQ=255)

                                                                                                          aTCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCg                                          >  7:353157/1‑147 (MQ=255)

                                                                                                           tCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCg                                          >  6:61737/1‑146 (MQ=255)

                                                                                                             acaACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGa                                      <  1:132415/148‑1 (MQ=255)

                                                                                                              caACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGAt                                     >  5:178831/1‑148 (MQ=255)

                                                                                                               aaCTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGa                                   <  7:245457/149‑1 (MQ=255)

                                                                                                               aaCTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGa                                   <  2:166833/149‑1 (MQ=255)

                                                                                                                 cttcttTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGaa                                  >  7:6992/1‑148 (MQ=255)

                                                                                                                  ttcttTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGTCGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGaaaa                                >  7:333023/1‑149 (MQ=255)

                                                                                                                  ttcttTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGaaa                                 >  6:235623/1‑148 (MQ=255)

                                                                                                                     tttGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATaa                             >  5:270077/1‑149 (MQ=255)

                                                                                                                       tGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATaac                            <  6:85712/148‑1 (MQ=255)

                                                                                                                       tGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATaa                             <  1:220640/147‑1 (MQ=255)

                                                                                                                       tGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATa                              <  8:316133/146‑1 (MQ=255)

                                                                                                                           tCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATaacaaa                         >  7:306660/1‑147 (MQ=255)

                                                                                                                              tggctggGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCGGCTATGGCGAAGATGAAAATAACAAACAGTa                    >  8:363645/1‑149 (MQ=255)

                                                                                                                                gctggGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTAt                   <  4:285840/148‑1 (MQ=255)

                                                                                                                                  tggGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCaa                >  4:268883/1‑149 (MQ=255)

                                                                                                                                    ggAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCGGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACg              >  8:344550/1‑149 (MQ=255)

                                                                                                                                     gAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACg              >  2:357855/1‑148 (MQ=255)

                                                                                                                                      aaTGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGt             >  8:354719/1‑148 (MQ=255)

                                                                                                                                       aTGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGTCACTGGCCTGGGCTTATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTaa           <  5:250332/149‑1 (MQ=255)

                                                                                                                                        tGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAAt          >  2:235282/1‑149 (MQ=255)

                                                                                                                                          aaaGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAAc               >  3:258117/1‑142 (MQ=255)

                                                                                                                                          aaaGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAAc               <  4:258117/142‑1 (MQ=255)

                                                                                                                                           aaGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTTTCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCAGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAATGcc       >  3:176989/1‑149 (MQ=255)

                                                                                                                                           aaGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGTTCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATg                                             <  3:162352/111‑1 (MQ=255)

                                                                                                                                           aaGTCCAGGCGGTGGATATGACCGAACTGCGTCGCACTATCGATCAGAAGATTTACGACGAAGCCGAAATGGAAATGGCACTGGCCTGCGCTGATAAAAACTACCGCTATg                                             >  4:162352/1‑111 (MQ=255)

                                                                                                                                             gTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAATGCCGa     <  6:32735/149‑1 (MQ=255)

                                                                                                                                                cAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAATGCCGAGc   <  8:306660/148‑1 (MQ=255)

                                                                                                                                                cAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAATGCCGAGCa  <  6:208220/149‑1 (MQ=255)

AAACTGCTGCGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTGGGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGGCTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAGATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTATGGCGAAGATGAAAATAACAAACAGTATCAACGTAATGCCGAGCA  >  NC_000913/2936055‑2936347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`