Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,807,094 T→C V242A (GTG→GCG)  pfkB → 6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,0940TC100.0% 363.8 / NA 101V242A (GTG→GCG) pfkB6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/55);  total (46/55)

Only 100 of 101 total aligned reads displayed.

GTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAT  >  NC_000913/1806952‑1807236
                                                                                                                                              |                                                                                                                                              
gTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGccac                                                                                                                                           >  7:115395/1‑148 (MQ=255)
gTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGcca                                                                                                                                            <  1:325279/147‑1 (MQ=255)
   aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTGGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCcaccac                                                                                                                                        <  4:298347/148‑1 (MQ=255)
    aTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCg                                                                                                                                      <  4:181120/149‑1 (MQ=255)
     tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCgg                                                                                                                                     >  3:194582/1‑149 (MQ=255)
     tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCgg                                                                                                                                     >  3:342161/1‑149 (MQ=255)
     tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCgg                                                                                                                                     >  4:71835/1‑149 (MQ=255)
           aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGaaaa                                                                                                                               <  3:352927/149‑1 (MQ=255)
             tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             >  7:23316/1‑149 (MQ=255)
               aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             >  4:98833/1‑147 (MQ=255)
               aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           <  5:89107/149‑1 (MQ=255)
               aCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           <  8:23316/149‑1 (MQ=255)
                 ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCa                                                                                                                           >  1:307054/1‑147 (MQ=255)
                   aGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCagag                                                                                                                        >  2:233356/1‑148 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             <  1:412264/139‑1 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             <  5:256698/139‑1 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             >  6:256698/1‑139 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGc                                                                                                                             >  2:412264/1‑139 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCg                                                                                                                   >  4:14094/1‑149 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCg                                                                                                                   >  8:216856/1‑149 (MQ=255)
                       ggACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCg                                                                                                                   >  3:293737/1‑149 (MQ=255)
                           gATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGGTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTgg                                                                                                               <  4:255087/149‑1 (MQ=255)
                              gTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGgcgc                                                                                                            >  4:311035/1‑149 (MQ=255)
                               tCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCt                                                                                                           >  7:70035/1‑149 (MQ=255)
                                  gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCCCCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGt                                                                                                        >  2:251571/1‑149 (MQ=255)
                                  gCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAACAGCCAGAGTACCGTTGGCGCTGGt                                                                                                        >  3:73146/1‑149 (MQ=255)
                                    aaaGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGa                                                                                                      >  7:20625/1‑149 (MQ=255)
                                      aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                                                                                    <  1:276438/149‑1 (MQ=255)
                                      aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                                                                                    <  6:186409/149‑1 (MQ=255)
                                      aGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                                                                                    <  7:233410/149‑1 (MQ=255)
                                        ccGCGCAGGAAATCGTTAAAAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGc                                                                                                  <  8:236447/149‑1 (MQ=255)
                                        ccGCGCAGGAAAGCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGTTGGCGCCACCACCGGTGTAAAGCCAGAGTACCGTTGGCGCTGGTGACAGc                                                                                                  <  6:353557/149‑1 (MQ=255)
                                         cgcgCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCa                                                                                                 <  3:14094/149‑1 (MQ=255)
                                            gcAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATgg                                                                                              >  4:263308/1‑149 (MQ=255)
                                              aGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTc                                                                                            <  3:263308/149‑1 (MQ=255)
                                              aGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGGGACAGCATGGTc                                                                                            >  4:16403/1‑149 (MQ=255)
                                                gAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCgg                                                                                          >  4:99975/1‑149 (MQ=255)
                                                   aTCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgcgc                                                                                       <  3:98833/149‑1 (MQ=255)
                                                    tCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGgcgc                                                                                       >  7:78866/1‑148 (MQ=255)
                                                      gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                     <  7:400362/148‑1 (MQ=255)
                                                      gTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGAt                                                                                    <  2:307054/149‑1 (MQ=255)
                                                      gTTAATAGCGGCAAGGCCAAACGGGTGGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGa                                                                                     <  5:182765/148‑1 (MQ=255)
                                                       ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                                                                                    >  4:165681/1‑132 (MQ=255)
                                                       ttAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACa                                                                                                    <  3:165681/132‑1 (MQ=255)
                                                        tAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGa                                                                                  >  5:139147/1‑149 (MQ=255)
                                                          aTAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGaca                                                                                <  8:78866/149‑1 (MQ=255)
                                                           tAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGCCGCCAGCACCTGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGacac                                                                               <  4:97075/149‑1 (MQ=255)
                                                           tAGAGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGacac                                                                               <  4:21752/149‑1 (MQ=255)
                                                              cGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTg                                                                             >  2:125002/1‑148 (MQ=255)
                                                                gCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGaaa                                                                          >  3:332621/1‑149 (MQ=255)
                                                                  aaGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACt                                                                        >  8:227617/1‑149 (MQ=255)
                                                                    ggCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                      <  4:332621/149‑1 (MQ=255)
                                                                    ggCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                      <  1:342060/149‑1 (MQ=255)
                                                                    ggCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTgg                                                                      <  5:332174/149‑1 (MQ=255)
                                                                        aaaCGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGATGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCTCTTGTGACAGCATGGTCGGCGCTATGACACTGAAACTGGCAg                                                                   >  3:180973/1‑148 (MQ=255)
                                                                          aCGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaa                                                                <  7:216856/149‑1 (MQ=255)
                                                                           cGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACGCTGAAACTGGCAGaaaa                                                               <  8:115395/149‑1 (MQ=255)
                                                                           cGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGaaaa                                                               <  4:194582/149‑1 (MQ=255)
                                                                            gggTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAAt                                                              <  4:184530/149‑1 (MQ=255)
                                                                                 gTCGTTTCCCTGTGTCCAAATGGAGCGCTGTGTGTTGATAGTGATAACTGTATTCAGGTGGCGACAACACCGGTGAAAAGCCAGAGTACCGTTGGGGGTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTTGCAGAAAATGCct                                                          <  8:157290/148‑1 (MQ=255)
                                                                                    gTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctct                                                      >  4:166629/1‑149 (MQ=255)
                                                                                    gTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc                                                       <  3:311035/148‑1 (MQ=255)
                                                                                    gTTTCCCTGGGTCCACAAGGAGCGCTGGGGGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCGGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCctctc                                                       <  4:352163/148‑1 (MQ=255)
                                                                                     tttCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGacac                                                                               <  1:351278/123‑1 (MQ=255)
                                                                                     tttCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGacac                                                                               >  2:351278/1‑123 (MQ=255)
                                                                                     tttCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCtt                                                     >  8:180697/1‑149 (MQ=255)
                                                                                         ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGCCACTGAAACTGGCAGAAAATGCCTCTCTTg                                                    >  1:391096/1‑146 (MQ=255)
                                                                                         ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTg                                                    >  3:264760/1‑146 (MQ=255)
                                                                                         ccTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTg                                                    >  7:194177/1‑146 (MQ=255)
                                                                                          cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                  >  7:245938/1‑147 (MQ=255)
                                                                                          cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGaa                                                  >  2:7801/1‑147 (MQ=255)
                                                                                          cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAaga                                                <  4:293737/149‑1 (MQ=255)
                                                                                          cTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAaga                                                <  3:71835/149‑1 (MQ=255)
                                                                                             ggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGAt                                             <  2:6120/149‑1 (MQ=255)
                                                                                             ggTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGAt                                             <  4:342161/149‑1 (MQ=255)
                                                                                                ccACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGCTGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGt                                          >  4:232157/1‑149 (MQ=255)
                                                                                                  acaAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTc                                        <  6:139147/149‑1 (MQ=255)
                                                                                                      ggAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGttt                                    <  8:245938/149‑1 (MQ=255)
                                                                                                       gAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGtttt                                   <  8:262195/149‑1 (MQ=255)
                                                                                                        agggcTGGTTTTTGATAGTGAAAACTGTATTCATGTGTCGTAAAAACCGGTGAAAATGCAGAGTTACTTTGTCGCTGTTGACAGAATGTTCTGCGCGATGACAATGAAACTGGCAGAAAATGAATCTATTGAAGAGATGTTTCGTTTTg                                  <  4:180973/146‑1 (MQ=255)
                                                                                                        aGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTg                                  <  1:251571/149‑1 (MQ=255)
                                                                                                          cgcTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGc                                <  3:166629/149‑1 (MQ=255)
                                                                                                           gcTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                               <  1:233356/149‑1 (MQ=255)
                                                                                                            cTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGt                              <  8:376951/149‑1 (MQ=255)
                                                                                                             tGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTggaga                              >  7:180738/1‑145 (MQ=255)
                                                                                                             tGGGTGTTGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCg                               >  2:110562/1‑147 (MQ=255)
                                                                                                                   ttGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCg                       >  7:16318/1‑149 (MQ=255)
                                                                                                                   ttGATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCg                       >  2:377431/1‑149 (MQ=255)
                                                                                                                     gATAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCgg                      >  4:305506/1‑148 (MQ=255)
                                                                                                                      aTAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     <  1:377431/148‑1 (MQ=255)
                                                                                                                      aTAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCggg                     <  8:70035/148‑1 (MQ=255)
                                                                                                                       tAGTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGa                   <  1:125002/149‑1 (MQ=255)
                                                                                                                         gTGAAAACTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAg                  <  1:110562/148‑1 (MQ=255)
                                                                                                                              aaCTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGc            <  8:20625/149‑1 (MQ=255)
                                                                                                                                cTGTATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCca          >  6:391849/1‑149 (MQ=255)
                                                                                                                                   tATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCcaca        <  7:227617/148‑1 (MQ=255)
                                                                                                                                   tATTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCacac       <  7:180697/149‑1 (MQ=255)
                                                                                                                                    aTTCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACt      >  3:115540/1‑149 (MQ=255)
                                                                                                                                      tCAGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTc     >  2:219888/1‑148 (MQ=255)
                                                                                                                                        aGGTGGCGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCTATTACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCCGCCACACTCAAt  >  6:387994/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGTCCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGAGTACCGTTGGCGCTGGTGACAGCATGGTCGGCGCGATGACACTGAAACTGGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAAT  >  NC_000913/1806952‑1807236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: