Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,890,075	IS5 (–) +4 bp	coding (1346‑1349/1416 nt)	tnaA →	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2290113	NA (NA)	45 (1.200)	28/240	0.5	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	3890075 =	0 (0.000)					coding (1346/1416 nt)	tnaA	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
*	?	NC_000913	3652036 =	NA (NA)	71 (1.890)	50/240	0.0	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 3890078	0 (0.000)					coding (1349/1416 nt)	tnaA	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent

CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289966‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTT  >  NC_000913/3890075‑3890218                                                                                                                                                                                                                                                                                                      CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 <  4:99414/149‑1    GACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAA                                                                                                                                              >  4:14630/1‑149          AAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATT                                                                                                                                        <  1:220860/149‑1                  TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGA                                                                                                                                          <  5:162764/139‑1                  TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGA                                                                                                                                          >  6:162764/1‑139                      CTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGTTAATCCTTTAagg                                                                                                                             <  1:86720/148‑4                              ACGGCGCTTACTGCTGACTTCACTGTCGGCGTAGGTAAGTGGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAAC                                                                                                                                     <  4:143864/132‑1                              ACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAAC                                                                                                                                     >  3:143864/1‑132                                  CGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAG                                                                                                                 >  5:199605/1‑148                                    CTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 <  1:124657/114‑1                                    CTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 >  2:124657/1‑114                                        CTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 <  5:42512/110‑1                                        CTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 >  6:42512/1‑110                                            TGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAA                                                                                                                                              <  7:159113/109‑1                                            TGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAA                                                                                                                                              >  8:159113/1‑109                                                TTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGAT                                                                                                                                         <  4:43664/110‑1                                                TTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGAT                                                                                                                                         >  3:43664/1‑110                                                     TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGC                                                                                              >  2:121965/1‑148                                                     TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT                                                                                                                                                <  7:2808/98‑1                                                     TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT                                                                                                                                                >  8:2808/1‑98                                                                        ATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAAT                                                                          <  1:121965/149‑1                                                                        ATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTT                                                                             <  4:122327/146‑1                                                                        ATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTT                                                                             >  3:122327/1‑146                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAAT                                                                      >  1:155213/1‑148                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAA                                                                           >  2:57820/1‑143                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAA                                                                           <  1:57820/143‑1                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAA                                                                                    <  2:53347/134‑1                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAA                                                                                    >  1:53347/1‑134                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 <  4:128930/73‑1                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                 >  3:128930/1‑73                                                                                TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTA                                                                  <  2:155213/149‑1                                                                                       CCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTG                                                           >  6:98067/1‑149                                                                                            TTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTAT                                                      <  6:199605/149‑1                                                                                                     TCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTA                                                                                                                                               >  8:7020/1‑51                                                                                                     TCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTA                                                                                                                                               <  7:7020/51‑1                                                                                                       CAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAG                                           >  1:70156/1‑149                                                                                                            GACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACT                                      >  8:58717/1‑149                                                                                                             ACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTC                                     <  7:58717/149‑1                                                                                                              CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCT                                    <  2:70156/149‑1                                                                                                               AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTT                                                                                       >  1:217512/1‑97                                                                                                               AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTT                                                                                       <  2:217512/97‑1                                                                                                                 ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATA                                                                     >  2:176200/1‑113                                                                                                                 ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATA                                                                     <  1:176200/113‑1                                                                                                                                              ACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTG                                                           <  6:171091/94‑1                                                                                                                                                CTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTT  >  5:112789/1‑149                                                                                                                                                                                                                                                                                                      CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289966‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTT  >  NC_000913/3890075‑3890218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.