Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,282,9270AG20.0% 58.6 / 10.3 30I318V (ATC→GTC) agaStagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base A (12/12);  new base G (3/3);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAG  >  NC_000913/3282822‑3283075
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tcCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTcc                                                                                                                                      <  1:198582/122‑1 (MQ=255)
tcCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTcc                                                                                                                                      >  2:198582/1‑122 (MQ=255)
     ccACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtc                                                                                                                 <  2:35296/138‑1 (MQ=255)
     ccACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACcgtc                                                                                                                 >  1:35296/1‑138 (MQ=255)
         ccTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGGGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGa                                                                                                  <  8:150684/149‑1 (MQ=255)
                          tgatcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAGGCAGCGACGATGTCGCTGctttc                                                                                                                                        <  3:116178/94‑5 (MQ=38)
                          tgatcttgatctGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATAGCAGCGGAAGGCAGCGACGATGTCGCTGcttta                                                                                                                                        >  4:116178/1‑90 (MQ=255)
                                            tgAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGcc                                                                >  1:34962/1‑148 (MQ=255)
                                                 ttCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACAtcgtcg                                                                                                                                                <  3:150361/63‑1 (MQ=255)
                                                 ttCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACAtcgtcg                                                                                                                                                >  4:150361/1‑63 (MQ=255)
                                                    gCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGtt                                                                                              <  2:24468/110‑1 (MQ=255)
                                                    gCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGtt                                                                                              >  1:24468/1‑110 (MQ=255)
                                                            aaCCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGAt                                               <  2:34962/149‑1 (MQ=255)
                                                                           gtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCcaca                                                                                                                                   >  1:7336/1‑50 (MQ=255)
                                                                           gtAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCcaca                                                                                                                                   <  2:7336/50‑1 (MQ=255)
                                                                               tcgccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                        >  7:78740/1‑105 (MQ=255)
                                                                               tcgccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                        <  8:78740/105‑1 (MQ=255)
                                                                                 gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                      <  7:24423/137‑1 (MQ=255)
                                                                                 gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCt                                      >  8:24423/1‑137 (MQ=255)
                                                                                 gccatcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCaa                          >  3:53891/1‑149 (MQ=255)
                                                                                   catcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAttt                                                                                  <  7:128780/91‑1 (MQ=255)
                                                                                   catcgccGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCAttt                                                                                  >  8:128780/1‑91 (MQ=255)
                                                                                          gcgGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGa                 >  5:104685/1‑149 (MQ=255)
                                                                                               cggcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGt                                                                                               >  5:123823/3‑66 (MQ=38)
                                                                                               cggcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGt                                                                                               <  6:123823/64‑1 (MQ=38)
                                                                                                 gcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                        >  5:146295/1‑87 (MQ=255)
                                                                                                 gcagcGACGATGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                        <  6:146295/87‑1 (MQ=255)
                                                                                                  cagcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                        >  2:136948/1‑86 (MQ=255)
                                                                                                  cagcGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGa                                                                        <  1:136948/86‑1 (MQ=255)
                                                                                                        cATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCa   <  6:104685/149‑1 (MQ=255)
                                                                                                         aTCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAg  >  3:30189/1‑149 (MQ=255)
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TCCAGCCACCCTTACACCCGTCAGTATGATCTTGATCTGCTGGCTGAACTTCGCCGTGACAACCAGGCAATGCGTGTAATCGCCATCGCCGCGGAAAGCAGCGACATCGTCGCTGCCGGTCCACATATCATCCTGCCACCGTCACGTCACTTTATCGACGTTGAGCAGGCATTTTGCTTCCTGATGTACGCCCAGACGTTTGCACTGATGCAGTCGCTGCACATGGGCAATACGCCGGATACCCCATCAGCCAG  >  NC_000913/3282822‑3283075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: