Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,309,0090CT20.0% 82.3 / 15.2 40intergenic (‑85/+24)nlpI/pnplipoprotein involved in osmotic sensitivity and filamentation/polynucleotide phosphorylase/polyadenylase
Reads supporting (aligned to +/- strand):  ref base C (16/16);  new base T (4/4);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGAACTTTCAC  >  NC_000913/3308865‑3309149
                                                                                                                                                |                                                                                                                                            
aCTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAg                                                                                                                                          >  6:137063/1‑149 (MQ=255)
      tCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAgg                                                                                                                                    <  2:211434/149‑1 (MQ=255)
         tGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGc                                                                                                                                  >  8:64438/1‑148 (MQ=255)
                          tCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                                >  7:120229/1‑149 (MQ=255)
                          tCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCg                                                                                                                >  3:173920/1‑149 (MQ=255)
                              aaCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCccgcc                                                                                                                                              <  7:167080/115‑1 (MQ=255)
                              aaCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCccgcc                                                                                                                                              <  6:70608/115‑1 (MQ=255)
                              aaCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCccgcc                                                                                                                                              >  8:167080/1‑115 (MQ=255)
                              aaCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCccgcc                                                                                                                                              >  5:70608/1‑115 (MQ=255)
                              aaCGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCt                                                                                                            <  8:120229/149‑1 (MQ=255)
                                  aaaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCTCCGCTGCGGCGGGGGGcttttaa                                                                                                                            >  1:106893/1‑123 (MQ=255)
                                  aaaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCTCCGCTGCGGCGGGGGGcttttaa                                                                                                                            <  2:106893/129‑7 (MQ=255)
                                  aaaCACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTc                                                                                                        <  5:137063/149‑1 (MQ=255)
                                        cAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCATCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCagcagc                                                                                                  >  7:120567/1‑149 (MQ=255)
                                              aaaaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                                   <  8:160240/126‑1 (MQ=255)
                                              aaaaaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                                   >  7:160240/1‑126 (MQ=255)
                                                 aaaGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTc                                                                                         <  4:173920/149‑1 (MQ=255)
                                                       ttCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgc                                                                                   >  6:91012/1‑149 (MQ=255)
                                                           tttCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGtgctg                                                                                 >  6:143070/1‑147 (MQ=255)
                                                              cccACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTgcagc                                                                             <  4:105750/148‑1 (MQ=255)
                                                                  cTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGtt                                                                        >  7:36659/1‑149 (MQ=255)
                                                                  cTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGtt                                                                        >  2:24817/1‑149 (MQ=255)
                                                                     ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                                                      <  7:64438/148‑1 (MQ=255)
                                                                     ccGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTga                                                                      <  1:24817/148‑1 (MQ=255)
                                                                        aaGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCaa                                                                                                                         >  2:16587/1‑94 (MQ=255)
                                                                        aaGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCaa                                                                                                                         <  1:16587/94‑1 (MQ=255)
                                                                        aaGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                                   >  4:79134/1‑100 (MQ=255)
                                                                        aaGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTAc                                                                                                                   <  3:79134/100‑1 (MQ=255)
                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCTCCGCTGCGGCgggggg                                                                                                                                   <  8:115304/79‑1 (MQ=38)
                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCTCCGCTGCGGCgggggg                                                                                                                                   >  7:115304/1‑79 (MQ=38)
                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCaaa                                                                                                                               >  1:161402/1‑83 (MQ=255)
                                                                             cACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCaaa                                                                                                                               <  2:161402/83‑1 (MQ=255)
                                                                              aCGGTTGAATGAACGTCCTGTTCCCGGTTGCTATCAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTca                                                                                                       >  2:87924/1‑106 (MQ=255)
                                                                              aCGGTTGAATGAACGTCCTGTTCCCGGTTGCTATCAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTca                                                                                                       <  1:87924/106‑1 (MQ=255)
                                                                                  ttGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGc                                                        <  5:143070/149‑1 (MQ=255)
                                                                                       tgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCTCCGCTGCGGCGGGGGGctt                                                                                                                                <  6:58032/72‑3 (MQ=37)
                                                                                       tgaaCGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCTCCGCTGCGGCGGGGGGctt                                                                                                                                >  5:58032/1‑70 (MQ=37)
                                                                                           cGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAAt                                               <  8:36659/149‑1 (MQ=255)
                                                                                                                cAAGGCATCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTg                          <  8:120567/149‑1 (MQ=255)
                                                                                                                 aaGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTgg                         >  4:132363/1‑149 (MQ=255)
                                                                                                                                   aaaaGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGAACt       >  3:53352/1‑149 (MQ=255)
                                                                                                                                    tttgCCCTCCGCTGCGGCGGGGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTcc             <  4:56361/139‑1 (MQ=255)
                                                                                                                                    tttgCCCTCCGCTGCGGCGGGGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTcc             >  3:56361/4‑142 (MQ=255)
                                                                                                                                        ccccccGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGAACTTTCAc  <  5:91012/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
ACTGCATCCTGCAAGCGTAAGTGCTGTCGCAACGAAACACCAGCGCAAAAAAGGCTTCATTTCCCACTCCCGAAGACCACGGTTGAATGAACGTCCTGTTCCCGGTTGCTAACAAGGCGTCCTGCCCGGTTAAAAGCCCCCCGCCGCAGCGGAGGGCAAATGGCAACCTTACTCGCCCTGTTCAGCAGCCGGAGCTTCCGGTGCTGCAGCAGGTTGAGACTGCTCAGTCGCTTCTTTAATGCTCAGACGGATACGGCCCTGGCGATCAACTTCCAGAACTTTCAC  >  NC_000913/3308865‑3309149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: