Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,471	A→C	I135M (ATT→ATG)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,471	0	A	C	100.0%	126.3 / NA	36	I135M (ATT→ATG)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (17/19);  total (17/19)

CTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGG  >  NC_000913/1261330‑1261607                                                                                                                                              |                                                                                                                                         cTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGtt                                                                                                                                   <  2:10184/149‑1 (MQ=255)                  cacgcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACt                                                                                                                  <  2:129910/149‑1 (MQ=255)                      cacaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCa                                                                                                              <  2:148469/149‑1 (MQ=255)                       acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAg                                                                                                             >  1:149093/1‑149 (MQ=255)                              cgccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACgg                                                                                                      >  2:170754/1‑149 (MQ=255)                               gccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGt                                                                                                     <  1:170754/149‑1 (MQ=255)                                               aCCACAATTGGGTTATCCAGATTCAGCTGCAGCAACTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACCTCGAAGAAACCCTGCATCTCTTCAGCGTGCAGATCCACTGTCAGCACACGGACAACACCGACGCTg                                                                                       >  7:52433/1‑147 (MQ=255)                                                  acaATTGTGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGagag                                                                                  <  8:52433/149‑1 (MQ=255)                                                         ggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTc                                                                            <  2:149093/148‑1 (MQ=255)                                                                               caTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                           <  6:34672/127‑1 (MQ=255)                                                                                 tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                    <  2:146342/100‑1 (MQ=255)                                                                                 tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                    >  1:146342/1‑100 (MQ=255)                                                                                  gTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACt                                                                                                                  >  5:78064/1‑85 (MQ=255)                                                                                  gTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACt                                                                                                                  <  6:78064/85‑1 (MQ=255)                                                                                   tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                  <  8:100137/148‑1 (MQ=255)                                                                                       ccAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                     <  5:11041/141‑1 (MQ=255)                                                                                       ccAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTg                                                     >  6:11041/1‑141 (MQ=255)                                                                                       ccAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                               >  6:82492/1‑147 (MQ=255)                                                                                       ccAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                               >  6:79865/1‑147 (MQ=255)                                                                                          gcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAac                                                                                                 >  3:84951/1‑94 (MQ=255)                                                                                          gcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAac                                                                                                 <  4:84951/94‑1 (MQ=255)                                                                                              gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                      <  5:79865/149‑1 (MQ=255)                                                                                              gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                      <  5:82492/149‑1 (MQ=255)                                                                                                tCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                                     >  1:158281/1‑148 (MQ=255)                                                                                                    gCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAcgc                                >  2:73872/1‑149 (MQ=255)                                                                                                        ccAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTc                                                                                                    <  6:112834/77‑1 (MQ=255)                                                                                                        ccAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGt                                                                             >  6:59843/1‑100 (MQ=255)                                                                                                           aaTACGTTATCAACCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACAAGCGGAACGGACGCGACGGTc                         >  2:77169/1‑149 (MQ=255)                                                                                                                       aCCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAc                                  <  6:128382/128‑1 (MQ=255)                                                                                                                       aCCGGAACGTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAc                                  >  5:128382/1‑128 (MQ=255)                                                                                                                               gTCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg      >  7:44125/1‑148 (MQ=255)                                                                                                                                tCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACg                            >  6:74948/1‑125 (MQ=255)                                                                                                                                tCGAAGAAACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACg                            <  5:74948/125‑1 (MQ=255)                                                                                                                                  gaagaaACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGc                                                                         <  7:83398/78‑1 (MQ=255)                                                                                                                                  gaagaaACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGc                                                                         >  8:83398/1‑78 (MQ=255)                                                                                                                                  gaagaaACCCTGCATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACAAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg  <  1:77169/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                         CTTAGCGATAGCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGG  >  NC_000913/1261330‑1261607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑