Predicted mutation
evidence seq id position mutation freq annotation gene description
JC JC NC_000913 1,757,082 IS5 (–) +4 bp 26.2% coding (1385‑1388/1413 nt) pykF → pyruvate kinase I

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)10 (0.110) 10/266 NT 28.8% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1757085 24 (0.270)coding (1388/1413 nt) pykF pyruvate kinase I
* ? NC_000913 1757082 =24 (0.270)7 (0.080) 6/266 NT 22.0% coding (1385/1413 nt) pykF pyruvate kinase I
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region

GGTTATCGATTAAATAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAATATTACAGGACGTGAACAGATGCGGTGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1757180‑1757082
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGAC  <  NC_000913/2102943‑2102820
                                                                                                                                                                                                                               
GGTTATCGATTAAATAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAAAATTACAGGACGTGAACAGATGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGa                                                                            >  7:392243/1‑148
 GTTATCGATTAAATAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAATATTACAGGACGTGAACAGCTGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGCGATCATGTTTGTCATCTGGAGC                                                                            >  2:90055/1‑148
          TAAATAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAATATTACAGGACGTGAACAGATGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAG                                                                  >  4:272610/1‑149
              TAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAATATTACAGGACGTGAACAGATGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTT                                                              >  8:96285/1‑149
              TAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAATATTACAGGACGTGAACAGATGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGT                                                               >  2:201938/1‑148
                                                       TTCACAAAAGCAATATTACAGGACGTGAACAGATGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTTAATTCA                     <  3:272610/149‑1
                                                                            GACGTGAACAGATGCGGTGTTAGGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCTTCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGAC  >  7:146010/1‑147
                                                                                                                                                                                                                               
GGTTATCGATTAAATAAAAAAAGCGCCCATCAGGGCGCTTCGATATACAAATTAATTCACAAAAGCAATATTACAGGACGTGAACAGATGCGGTGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1757180‑1757082
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGAC  <  NC_000913/2102943‑2102820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.