| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,930 | C→A | 24.1% | P671Q (CCG→CAG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,930 | 0 | C | A | 24.1% | 44.3 / 2.6 | 30 | P671Q (CCG→CAG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (15/7); new base A (6/2); total (21/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGT > NC_000913/1708802‑1709074 | ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGGAACGGGGGAGCGCACCAGGCTACTGCGGACCCaggag > 5:368122/1‑149 (MQ=14) gTGCCAAAGCCCGCAAGCCGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAgaagaa < 7:64419/149‑1 (MQ=255) cAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGc > 7:65349/1‑149 (MQ=255) aaCAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCACCCGGCTAATGCGGACCCAGAAGAACAGGGTGATCCGCGCAAAGATgactgc > 1:21889/1‑144 (MQ=14) tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGCGCCCACGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGACGCGGCTa > 5:79387/1‑149 (MQ=14) acaacaacCGGTCGATCCGCGCAAATCCGCCGGCGTATCCGCTATTTCCCGATCCAATGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt < 5:160854/140‑1 (MQ=21) aGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGAAAACGGGAACAGCAACCGGCTAATGCGGACCCAGAAGAACAGGTTGATAAGCGCAAAGCTGCAGTGGCAGCGGATATTGCAagcg > 8:193649/1‑149 (MQ=14) aGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGCGCGAAAGCCCTCAAACCGGCACAGCAACAGGATAATGCGGAGCCATAAAAACAATTAAATCAGCGCAAAGCTACAGTGGCCTCGTCTATTGCAcgc > 8:100758/1‑148 (MQ=2) aGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGGAAAAGGGAAAGGCAACAGGCAAATGAGGAGCAAGAAGAGCAGGTGGAACCGCGAAAAGATACCGTCGAAGCGGCGATTgagagcg > 6:160854/1‑143 (MQ=14) aacaacAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaa < 2:266449/98‑1 (MQ=255) aacaacAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAACCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCCCGAAAAGCAGCCGTCGAAGCGGCAATAGCAcgcac > 7:266189/1‑146 (MQ=14) aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAACCAGAAGAACAGGTTGAGACGCGCAAAGCACCCGTCGGAGCTCCTCTTCCACGCGCCaat > 5:68539/1‑148 (MQ=14) aacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCAAGAATAACAGTTTGATCCGCGAAAAGCTGCCGTAGACGCGGCTATTGCCCGAGCaaat > 8:104227/1‑148 (MQ=14) caGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAACGGCTAATGCGGAGCCAGAAGAACAGGTTGATCAGCGCAAAGCTGCAGTAGCCGCGGCTATTGCACGCGCAAAGGc > 4:147394/1‑149 (MQ=17) gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGt < 6:393016/98‑1 (MQ=255) gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGt > 5:393016/1‑98 (MQ=255) gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCCGCGGCGATTGCCCGCGCCAAAGCACg > 1:226164/1‑149 (MQ=255) gATCCTCGCAACTCCGCCTTCGAATCCTTTATTGCCATAGCCAAATCGAGGATCTTGGTAGATCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCaa < 2:21889/149‑1 (MQ=18) aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTaa < 2:24531/73‑1 (MQ=255) aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTaa > 1:24531/1‑73 (MQ=255) gTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGACGCGGCTATTGCCCGCGCACAAGCACTCAAGGGGGACCAGCAAAAgg > 4:274266/1‑148 (MQ=14) cGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt > 7:405200/1‑103 (MQ=255) cGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt < 8:405200/103‑1 (MQ=255) gCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCCCGCGCCAACGCCCGAAAGCGGGCACAGCACCAGGCTATTGCGGt > 8:96467/1‑149 (MQ=21) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGACGCGGCTATTGCACGCGCCAATGCAAGAAAGCAGGACCAGCAAAAGGATAAAGAGGAACCaga > 7:312235/1‑148 (MQ=21) aaaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa < 7:319856/85‑1 (MQ=11) aaaCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAACGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCcgcg > 5:9625/1‑149 (MQ=255) gAACAGCAACAGGCTAATGCGGACCCAGAACAACAGGTAGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGAACGCGCCaaa > 2:373044/1‑84 (MQ=11) ccAACAGGCTAATGCGGAGCCAGTCTAACTCGTTGAGCCGCGCTAAGCTGCGGTCGAAGCGGCTATTGCACGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAg < 3:361650/148‑1 (MQ=17) cAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCAGCAACCGCGAACAGCAACAGGCTAAAGCGGTACCAGAAGACCAGGTAAAAACGCCCAAAGCGGCAGt > 4:2197/1‑149 (MQ=25) | CCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGT > NC_000913/1708802‑1709074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |