Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,367,945	G→T	16.1%	G292V (GGT→GTT)	arnC →	undecaprenyl phosphate‑L‑Ara4FN transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,367,945	0	G	T	16.1%	99.4 / 3.5	44	G292V (GGT→GTT)	arnC	undecaprenyl phosphate‑L‑Ara4FN transferase
Reads supporting (aligned to +/- strand):  ref base G (18/18);  new base T (2/6);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCGATTGGAGGTTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCC  >  NC_000913/2367798‑2368093                                                                                                                                                    |                                                                                                                                                     ttGCGATTGGAGGGTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTGGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCgg                                                                                                                                                      <  1:396623/148‑1 (MQ=255)  tGCGATTGGAGGTTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGt                                                                                                                                                     <  4:173414/148‑1 (MQ=255)   gCGATTGGAGGTTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTg                                                                                                                                                    <  3:280027/148‑1 (MQ=255)     gATTGGAGGTTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAat                                                                                                                                                 <  6:212655/149‑1 (MQ=255)          gAGGGTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACATTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTTCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATc                                                                                                                                            >  1:105060/1‑149 (MQ=255)                   gCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATc                                                                                                                                   >  4:2011/1‑149 (MQ=255)                   gCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAACGCGTCTATATGCTATTTGCAGTGCTGTTTAATATTATTGTCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGCTc                                                                                                                                   >  7:404267/1‑149 (MQ=255)                     aTTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTa                                                                                                                                 <  4:324155/149‑1 (MQ=255)                     aTTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTa                                                                                                                                 <  8:263992/149‑1 (MQ=255)                      ttGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCTGCAAGGGATTACTCGGTGAATATATCGGCAGGATCTa                                                                                                                                 >  6:78671/1‑148 (MQ=255)                               tgctgGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCGTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTGATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTc                                                                                                                       >  3:114279/1‑149 (MQ=255)                               tgctgGTGATTTTACGCCTGACCTTCGGACCACAATCGGCGGCAGAACGCGTCTTTATGCTATTTGCCGAGCTGTTTACTTTTATTGGAGCTCAGTTTATCGGCATGCGATAACTCGGTGAATATATCGGCAGGATCTACACCGATGTc                                                                                                                       >  4:99709/1‑149 (MQ=255)                                  tgGTGATTTTAAGCCTGACCTTCGGAACACAATGGTCGGAAGAAGGCGTCTTTATGCTATTTGCGGTGCTGTTTACTTTTATTGGCGATCAGTTTATCTGCATGTGATTACTCGTTGAATATATCGGCAGGAGCTACACCGCTGTCcgc                                                                                                                    >  6:109039/1‑149 (MQ=255)                                    gTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCgcgc                                                                                                                  >  8:347117/1‑149 (MQ=255)                                            aCGCCTGCCCTTCCGACCACCATGGGCCGCATTAGGCGGCTTTATGCTATTTGCCGTGCTGGGTACTTTTATTGTCGCTCAGTTTATAGTTTTTTTTTTTAATGTTTATTATATCTGCTTGATATACACCGATGTCCGCGCCCGcccc                                                                                                           <  6:379487/148‑1 (MQ=255)                                              gCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGc                                                                                                        >  4:13323/1‑149 (MQ=255)                                                  gACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTAtt                                                                                                    >  4:51581/1‑149 (MQ=255)                                                   aCCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTAttt                                                                                                   >  2:152700/1‑149 (MQ=255)                                                   aCCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTCCTGTTTACTTTTATTGGCGGTCAGTTTAACGGAATGGTATAACGCGGTGAATTTATGGGCAGGATCTCCACCGATGGCCGCGCCCGCCCCCGATtttt                                                                                                   >  4:73454/1‑149 (MQ=255)                                                     cTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTg                                                                                                 <  3:56855/149‑1 (MQ=255)                                                        cGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGtt                                                                                               <  1:152700/148‑1 (MQ=255)                                                         ggACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGATTTTTATTACTTGTTTATTATATCTGCTTGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTc                                                                                              <  6:67019/148‑1 (MQ=255)                                                             cacaATGGGCGGCAGATCGCGTCTTAATGCTAATTGACGTGCTGTTTACTTTAAATCTCGCTAAGGTAATCGGCTTGGGATTACTCGTTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCaa                                                                                         <  2:319095/149‑1 (MQ=255)                                                                                  tCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAg                                                                    <  3:13323/149‑1 (MQ=255)                                                                                            aTTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGTTGAATATATCGGCAGGATCTAAACCGATGTACGCGACCGCCCCAGCTATTTTGGTCAGCAAGTAATCCGTAAATCCAGCAAGGAAAATGaa                                                           >  8:373068/1‑148 (MQ=255)                                                                                            aTTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGaa                                                           >  4:26686/1‑148 (MQ=255)                                                                                             tttGCCGTGCTTTTTACTTGTATGGGCGCTAAGTTTATCGTCATTGGATTACTCGGTGAATATATCGGCAGGATATACACCTATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCAAGCAAGGAAAATGaat                                                          <  7:373068/148‑1 (MQ=255)                                                                                             gttGCCCTGCTGTTAAAGATTCTTCGCGCTCAGTTTATCGGCATGCGCTTACTCGGTGAAAAAATCGGCAGGAGCTACACCGATGGCCGCGCCCCCCCCCTCTATTTTGTTCACCAAGTTATCCGTCCATCCCCCAAGGAAAATGaata                                                         <  5:109039/148‑1 (MQ=255)                                                                                                  cGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGaat                                                          <  2:360977/143‑1 (MQ=255)                                                                                                  cGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGaat                                                          >  1:360977/1‑143 (MQ=255)                                                                                                    tGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGaaa                                                   >  8:131497/1‑148 (MQ=255)                                                                                                    agCTGTTTACTTTTATTGGCGCTCATTTTATAGTCATTGTATTAATTGTTTATTATATCTGCTTTATCTAAACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCAGTCCATCCAGCAAGGAAAATGAATAATGaaaa                                                  <  2:380922/148‑1 (MQ=255)                                                                                                             ctattATTGGCGCTCAGCTTATCGGCATTTGTTTACTCGTTGAATATATCTGCTTGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGttt                                         <  8:404267/146‑1 (MQ=255)                                                                                                             cTTTTATTGGCGCTCAGTTTATCGTCATGTGATTACTAGTTTAATATATCTGATTGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGttt                                         <  2:105060/149‑1 (MQ=255)                                                                                                                ttATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGtttt                                        <  3:2011/147‑1 (MQ=255)                                                                                                                  aTTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGc                                     <  7:131497/148‑1 (MQ=255)                                                                                                                         cTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCAc                              >  3:20353/1‑148 (MQ=255)                                                                                                                             gTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGatat                         <  7:108433/149‑1 (MQ=255)                                                                                                                              tttATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGtttt                                        >  2:120014/1‑133 (MQ=255)                                                                                                                              tttATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGtttt                                        <  1:120014/133‑1 (MQ=255)                                                                                                                                tATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATgg                       <  3:26686/148‑1 (MQ=255)                                                                                                                                  tCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGa                     <  4:20353/148‑1 (MQ=255)                                                                                                                                      cATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGACGTTTTTGCCTACCACGATATCGGATGcc                 >  3:295462/1‑148 (MQ=255)                                                                                                                                           gATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCAc                              <  6:395150/130‑1 (MQ=255)                                                                                                                                           gATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCAc                              >  5:395150/1‑130 (MQ=255)                                                                                                                                                    gTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGccc  >  8:215106/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TTGCGATTGGAGGTTTTAGCATTGCGGTGCTGCTGGTGATTTTACGCCTGACCTTCGGACCACAATGGGCGGCAGAAGGCGTCTTTATGCTATTTGCCGTGCTGTTTACTTTTATTGGCGCTCAGTTTATCGGCATGGGATTACTCGGTGAATATATCGGCAGGATCTACACCGATGTCCGCGCCCGCCCCCGCTATTTTGTTCAGCAAGTTATCCGTCCATCCAGCAAGGAAAATGAATAATGAAAACCGTCGTTTTTGCCTACCACGATATGGGATGCCTCGGTATTGAAGCCC  >  NC_000913/2367798‑2368093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑