Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,569,416 G→A 5.2% C32C (TGC→TGT eutG ← ethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,569,4160GA 5.2% 249.4 / 2.1 76C32C (TGC→TGTeutGethanol dehydrogenase involved in ethanolamine utilization; aldehyde reductase
Reads supporting (aligned to +/- strand):  ref base G (31/41);  new base A (2/2);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATGGCGATACCTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCAT  >  NC_000913/2569272‑2569563
                                                                                                                                                |                                                                                                                                                   
cATGGCGATACCTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCaaa                                                                                                                                                 <  2:280670/149‑1 (MQ=255)
 aTGGCGATAGCTTTAACTGTCTGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTTATTCAAAAAGCTGTCTGCCATCACGAACAGATTTTTCAGCCAACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAg                                                                                                                                                <  5:54946/149‑1 (MQ=255)
  tGGCGATACCTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGc                                                                                                                                               >  7:95782/1‑149 (MQ=255)
       aTACCTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCGGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCAc                                                                                                                                          <  5:211938/149‑1 (MQ=255)
          ccTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCCCGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCg                                                                                                                                        <  6:377933/148‑1 (MQ=255)
           cTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCACAAAGCGTCACCGGt                                                                                                                                      >  3:295297/1‑149 (MQ=255)
                aCGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGaa                                                                                                                                  <  6:171648/148‑1 (MQ=255)
                aCGGTCAGGCTACGAGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCTCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAAc                                                                                                                                 >  8:332992/1‑149 (MQ=255)
                      aGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTaaa                                                                                                                           <  1:158027/149‑1 (MQ=255)
                         cTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGt                                                                                                                        >  4:321340/1‑149 (MQ=255)
                           aCGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGttt                                                                                                                      >  5:19772/1‑149 (MQ=255)
                           aCGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGttt                                                                                                                      >  7:228454/1‑149 (MQ=255)
                            cgcgTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGttt                                                                                                                      <  7:326500/148‑1 (MQ=255)
                            cgcgTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGttt                                                                                                                      <  5:48478/148‑1 (MQ=255)
                              cgTGAGCCCGGCGGGCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCTCAACTGCTCTCCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTa                                                                                                                   <  7:299235/149‑1 (MQ=255)
                               gTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTAc                                                                                                                  <  7:41470/149‑1 (MQ=255)
                                     ccGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCATCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTg                                                                                                            <  3:270397/149‑1 (MQ=255)
                                     ccGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTg                                                                                                            >  2:149844/1‑149 (MQ=255)
                                     ccGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTg                                                                                                            <  7:67325/149‑1 (MQ=255)
                                      cggcggTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGc                                                                                                           <  8:216536/149‑1 (MQ=255)
                                       ggcggTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTg                                                                                                            >  8:16227/1‑147 (MQ=255)
                                        gcggGCAGCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAg                                                                                                         <  5:186871/149‑1 (MQ=255)
                                              aTCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCa                                                                                                    <  4:220961/148‑1 (MQ=255)
                                              aTCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCa                                                                                                    <  1:345489/148‑1 (MQ=255)
                                              aTCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAg                                                                                                   >  7:73844/1‑149 (MQ=255)
                                               tCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAgg                                                                                                  <  3:198471/149‑1 (MQ=255)
                                               tCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAgg                                                                                                  <  4:163011/149‑1 (MQ=255)
                                                cccTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAggg                                                                                                 >  5:52682/1‑149 (MQ=255)
                                                cccTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGATTGCTGTCCGCAACTGCTCCCCCAGCACGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAggg                                                                                                 >  1:112617/1‑149 (MQ=255)
                                                  ctgcctgATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGc                                                                                                                                               >  1:169462/1‑101 (MQ=255)
                                                  ctgcctgATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGc                                                                                                                                               <  2:169462/101‑1 (MQ=255)
                                                      ctgATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTAt                                                                                              >  1:10926/1‑146 (MQ=255)
                                                      ctgATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTAt                                                                                              <  2:10926/146‑1 (MQ=255)
                                                       tgATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGaa                                                                                          >  4:243801/1‑149 (MQ=255)
                                                        gATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAAc                                                                                         >  3:40863/1‑149 (MQ=255)
                                                             aaaaaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCACCTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGACCGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCt                                                                                     >  4:113117/1‑148 (MQ=255)
                                                              aaaaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGa                                                                                   <  6:158768/149‑1 (MQ=255)
                                                              aaaaCCTGTCTGCCATCACGACCAGCTGATTCATCCCACGCGTTTCCGCTTGCTGTCCGCAACTGCTCAACGACCCCTGACCGCAAAGCGTCACCGGGGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGa                                                                                   <  6:372576/149‑1 (MQ=255)
                                                                aaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGaaa                                                                                 <  3:190451/149‑1 (MQ=255)
                                                                      tCTGCCATCACGAACAGATGTTTCAGCGCACGCGTTTGCGCTTGCTGTCCGCAACTGCGCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAgc                                                                             <  8:49602/147‑1 (MQ=255)
                                                                      tCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCTCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAgcgc                                                                           <  7:332992/149‑1 (MQ=255)
                                                                      tCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAgcgc                                                                           >  2:257640/1‑149 (MQ=255)
                                                                          ccATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGt                                                                        <  3:18954/148‑1 (MQ=255)
                                                                           cATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACAGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACACGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCt                                                                      >  8:84914/1‑149 (MQ=255)
                                                                             tCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGaa                                                                                          >  8:8648/1‑127 (MQ=255)
                                                                             tCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGaa                                                                                          <  7:8648/127‑1 (MQ=255)
                                                                             tCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGcgcg                                                                          >  8:200394/1‑143 (MQ=255)
                                                                             tCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGcgcg                                                                          <  7:200394/143‑1 (MQ=255)
                                                                             tCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTAGCTGACCGCAACTGCTCACCGAGCCCGGACCACAAAGCGTCACCGGTGGAACGCTAAATGTATTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTg                                                                     >  3:108662/1‑148 (MQ=255)
                                                                             tCACGAACAAATATTTCAGCCCACGCGTTTGCGCTAGCTGTCCGCAACTGCTCACCGAGCCCGGACAGCAAAGAGACACCGGTGGAACGCAAAAAGTATTTACCCGTTGCATATTCATGGTATAGAACTCATGAAAGAGCGCGGTCTg                                                                     >  4:66326/1‑148 (MQ=255)
                                                                               aCGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGGCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCaa                                                                  <  2:161693/149‑1 (MQ=255)
                                                                                 gAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAAtt                                                                >  5:331244/1‑149 (MQ=255)
                                                                                      gATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAttt                                                           <  8:171159/149‑1 (MQ=255)
                                                                                       aTGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtttt                                                          >  4:132922/1‑149 (MQ=255)
                                                                                           ttCAGCCCTCGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCACAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCa                                                       <  4:108662/148‑1 (MQ=255)
                                                                                                cccACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGc                                                 <  3:139165/149‑1 (MQ=255)
                                                                                                  cACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGccc                                               <  4:40863/149‑1 (MQ=255)
                                                                                                    cgcgTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCt                                             >  8:368382/1‑149 (MQ=255)
                                                                                                        tttGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGc                                         <  3:321340/149‑1 (MQ=255)
                                                                                                           gcgcTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCttt                                      >  5:5773/1‑149 (MQ=255)
                                                                                                            cgcTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTtc                                     <  1:25973/149‑1 (MQ=255)
                                                                                                              cTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTtctc                                   <  1:257640/149‑1 (MQ=255)
                                                                                                                tGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTtctctc                                 >  4:95275/1‑149 (MQ=255)
                                                                                                                  cTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGc                               >  2:394763/1‑149 (MQ=255)
                                                                                                                     tCCGCAACTGCTCACCGAGCCCGGACCACAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCa                            <  4:295297/149‑1 (MQ=255)
                                                                                                                       cGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                           >  3:275192/1‑148 (MQ=255)
                                                                                                                        gCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCacta                         <  8:95782/149‑1 (MQ=255)
                                                                                                                        gCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCacta                         >  3:208056/1‑149 (MQ=255)
                                                                                                                            cTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTgg                     <  7:16227/149‑1 (MQ=255)
                                                                                                                               cTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGa                  >  7:296280/1‑149 (MQ=255)
                                                                                                                                  aCCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGAt                 <  3:243801/147‑1 (MQ=255)
                                                                                                                                  aCCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATc                <  1:149844/148‑1 (MQ=255)
                                                                                                                                  aCCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCg               >  8:218975/1‑149 (MQ=255)
                                                                                                                                               cGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATc                <  3:229528/135‑1 (MQ=255)
                                                                                                                                               cGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATc                >  4:229528/1‑135 (MQ=255)
                                                                                                                                               cGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCAt  <  6:270160/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CATGGCGATACCTTTAACGGTCAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCGGGGTCAT  >  NC_000913/2569272‑2569563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: