Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,975,601 T→G 5.2% E138D (GAA→GAC aas ← fused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,975,6010TG 5.2% 243.0 / 4.3 77E138D (GAA→GACaasfused 2‑acylglycerophospho‑ethanolamine acyl transferase/acyl‑acyl carrier protein synthetase
Reads supporting (aligned to +/- strand):  ref base T (39/34);  new base G (2/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TTTCGCCAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTCCACCAGACGTACCAGAT  >  NC_000913/2975453‑2975749
                                                                                                                                                    |                                                                                                                                                    
tttCGCCAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCt                                                                                                                                                      >  3:38665/1‑149 (MQ=255)
    ggcAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGTAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCATAAGTTCCGCCCCTTCaa                                                                                                                                                  <  2:95992/147‑1 (MQ=255)
    gCCAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCaa                                                                                                                                                  <  7:322356/149‑1 (MQ=255)
      cAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTc                                                                                                                                                    <  7:260563/145‑1 (MQ=255)
      cAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTc                                                                                                                                                    >  8:260563/1‑145 (MQ=255)
            ttttGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGcaca                                                                                                                                          <  1:106619/149‑1 (MQ=255)
              ttGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAg                                                                                                                                         <  3:222719/148‑1 (MQ=255)
                  gACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATa                                                                                                                                    <  3:335495/149‑1 (MQ=255)
                      gTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCg                                                                                                                                <  3:138564/149‑1 (MQ=255)
                      gTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCg                                                                                                                                >  4:138564/1‑149 (MQ=255)
                         aCGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTg                                                                                                                             >  5:379954/1‑149 (MQ=255)
                         aCGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTg                                                                                                                             <  1:200490/149‑1 (MQ=255)
                          cGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGc                                                                                                                            <  6:379954/149‑1 (MQ=255)
                                cgcgGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCGTCAATACGCACAGGAATAACCGTTGCACCAGa                                                                                                                      >  7:65220/1‑149 (MQ=255)
                                cgcgGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCGTCAATACGCACAGGAATAACCGTTGCACCAGa                                                                                                                      >  7:58506/1‑149 (MQ=255)
                                     cgcATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTcg                                                                                                                 >  4:277862/1‑149 (MQ=255)
                                      gcATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTcgc                                                                                                                <  2:93523/149‑1 (MQ=255)
                                          ccGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTcgccgc                                                                                                             >  5:158835/1‑148 (MQ=255)
                                           cGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGa                                                                                                           <  3:332885/149‑1 (MQ=255)
                                              cATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACaa                                                                                                        <  3:277862/149‑1 (MQ=255)
                                              cATCGCCACCTCCGTTGGTGGCAAAATATGCAGACTAATTTGCGGGAACAAGCGACGTTTAACCAGACATTTCAGGCGGCTGAAGAGCGTAAGTTCCGCCCCTTCAATACGCACAGGAAAAACCGTTGCACCACACTACGCCGCGACa                                                                                                         >  2:115298/1‑148 (MQ=255)
                                                tCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACaaaa                                                                                                      >  8:169608/1‑149 (MQ=255)
                                                     aCCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCcgc                                                                                                 >  7:117335/1‑149 (MQ=255)
                                                      ccTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCcgcg                                                                                                <  8:111461/149‑1 (MQ=255)
                                                       cTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCgcgc                                                                                               >  6:77700/1‑149 (MQ=255)
                                                              ggtggCAAAATATGCAGAGTAATTTGCGGGATCAAGCGACGTTTATCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCGCCTTCAATACGCACAGGAATAACCGTTGCACCAGCCTTCGCCGCGACAAAACCCGCGCCATCg                                                                                          >  3:306454/1‑147 (MQ=255)
                                                               gtggCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGa                                                                                                           >  8:79836/1‑129 (MQ=255)
                                                               gtggCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGa                                                                                                           <  7:79836/129‑1 (MQ=255)
                                                                    aaaaTATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAtttt                                                                                  >  6:294620/1‑149 (MQ=255)
                                                                    aaaaTATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAtttt                                                                                  >  1:181309/1‑149 (MQ=255)
                                                                     aaaTATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTtc                                                                                 <  4:214211/149‑1 (MQ=255)
                                                                      aaTATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTtca                                                                                >  5:95005/1‑149 (MQ=255)
                                                                      aaTATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGCTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTtca                                                                                <  8:303515/149‑1 (MQ=255)
                                                                       atatTCATAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTATCAGGCGGCTGAAGTTCGTAAGTTCCGCCCCTTCAATAAGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCAATCGTAGATTTtcat                                                                               <  3:269392/149‑1 (MQ=255)
                                                                       atatGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTtcat                                                                               <  6:192722/149‑1 (MQ=255)
                                                                           gCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAATCCCGCGCCATCGTAGATTTTCATCAGc                                                                           >  3:329063/1‑149 (MQ=255)
                                                                            cAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCGTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGc                                                                           <  8:65220/148‑1 (MQ=255)
                                                                            cAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCGTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGc                                                                           <  8:58506/148‑1 (MQ=255)
                                                                             agagTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGa                                                                         <  5:77700/149‑1 (MQ=255)
                                                                               agTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGc                                                                       <  7:307589/149‑1 (MQ=255)
                                                                               agTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGc                                                                       >  5:356549/1‑149 (MQ=255)
                                                                                 tAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCt                                                                     >  8:369137/1‑149 (MQ=255)
                                                                                   aTTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGt                                                                   <  1:409080/149‑1 (MQ=255)
                                                                                   aTTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGt                                                                   >  3:51874/1‑149 (MQ=255)
                                                                                    tttGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAtt                                                                                    >  5:47475/1‑131 (MQ=255)
                                                                                    tttGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGAtt                                                                                    <  6:47475/131‑1 (MQ=255)
                                                                                    tttGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTc                                                                  <  2:258699/149‑1 (MQ=255)
                                                                                        cGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtg                                                               <  6:157621/148‑1 (MQ=255)
                                                                                         gggAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtggt                                                             <  4:38665/149‑1 (MQ=255)
                                                                                           gAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGa                                                           >  5:56897/1‑149 (MQ=255)
                                                                                            aaCAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGAt                                                          <  3:22797/149‑1 (MQ=255)
                                                                                                          aaCCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAggg                                             >  1:26878/1‑148 (MQ=255)
                                                                                                            ccAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGaa                                           >  8:292905/1‑148 (MQ=255)
                                                                                                                aCCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtggt                                                             >  7:236032/1‑126 (MQ=255)
                                                                                                                aCCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCgtggt                                                             <  8:236032/126‑1 (MQ=255)
                                                                                                                 ccTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATAAGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcac                                     <  5:328025/149‑1 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGCGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcacca                                   >  4:66929/1‑149 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcacca                                   >  6:24953/1‑149 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcacca                                   >  8:394875/1‑149 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcacca                                   >  5:325123/1‑149 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcacca                                   >  2:39471/1‑149 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcac                                     <  4:151918/147‑1 (MQ=255)
                                                                                                                   tttCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATcac                                     >  3:151918/1‑147 (MQ=255)
                                                                                                                         gcggcTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTc                             <  7:169608/149‑1 (MQ=255)
                                                                                                                              tGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGccc                        >  6:167044/1‑149 (MQ=255)
                                                                                                                               gAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCt                       >  4:372118/1‑149 (MQ=255)
                                                                                                                               gAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCt                       >  1:365322/1‑149 (MQ=255)
                                                                                                                                taGTGCGTAAGTTCCGCCCCTTCATTACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCGGCGCCATAGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGGCCTg                      <  4:164852/148‑1 (MQ=255)
                                                                                                                                    gCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTc                   >  2:38881/1‑148 (MQ=255)
                                                                                                                                     cGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGccc                        >  6:99703/1‑142 (MQ=255)
                                                                                                                                     cGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGccc                        <  5:99703/142‑1 (MQ=255)
                                                                                                                                      gTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGCCTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCCGCGAGCCTGGCGTGGTGATGCGTCCATCAGGGAAAATCACCACTGGTCGGCCCTGTTccac                <  5:299319/149‑1 (MQ=255)
                                                                                                                                      gTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACAGGTCGGCCCTGTTccac                >  4:87504/1‑149 (MQ=255)
                                                                                                                                          gTTCCGCCCCTTCAATACGCACAGGAATAACCGTTCCCCCTGACTTAGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTGCCCCAGa            >  8:234668/1‑149 (MQ=255)
                                                                                                                                           ttCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTCCACCAGAc           <  2:58361/149‑1 (MQ=255)
                                                                                                                                                ccccTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGTCCTGTTCCACCAGACGTAc       >  3:54069/1‑148 (MQ=255)
                                                                                                                                                  ccTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTCCACCAGACGTAc       >  5:337800/1‑146 (MQ=255)
                                                                                                                                                   cTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTCCACCAGACGTAc       >  5:272796/1‑145 (MQ=255)
                                                                                                                                                    ttCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTCCACCAGACGTACCAGAt  >  5:184250/1‑149 (MQ=255)
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TTTCGCCAGCGATTTTGCGACGGTCACGGGCACGCGGCGCATCCGGCATCGCCACCTGCGTTGGTGGCAAAATATGCAGAGTAATTTGCGGGAACAAGCGACGTTTAACCAGACCTTTCAGGCGGCTGAAGTGCGTAAGTTCCGCCCCTTCAATACGCACAGGAATAACCGTTGCACCAGACTTCGCCGCGACAAAACCCGCGCCATCGTAGATTTTCATCAGCGAGCCTGTCGTGGTGATGCGTCCTTCAGGGAAAATCACCACTGGTCGGCCCTGTTCCACCAGACGTACCAGAT  >  NC_000913/2975453‑2975749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: