Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,056,551 T→A 6.4% V104E (GTG→GAG)  fau → 5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,056,5510TA 6.4% 220.1 / 4.2 74V104E (GTG→GAG) fau5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (3/2);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

AACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCAT  >  NC_000913/3056404‑3056699
                                                                                                                                                   |                                                                                                                                                    
aacTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTg                                                                                                                                                     >  7:266345/1‑149 (MQ=255)
  ctctGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGc                                                                                                                                                    >  3:315999/1‑148 (MQ=255)
   tctGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTa                                                                                                                                                  >  7:409795/1‑149 (MQ=255)
     tGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTa                                                                                                                                                  <  8:409795/147‑1 (MQ=255)
     tGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGACCCATGAGCCAAAATTGGATGTGCGTGACGTGCTAcc                                                                                                                                                >  5:70033/1‑149 (MQ=255)
       gcgcgcCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTAcccc                                                                                                                                              >  4:225947/1‑149 (MQ=255)
       gcgcgcCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTAcccc                                                                                                                                              >  4:141572/1‑149 (MQ=255)
         gcgcCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCtt                                                                                                                                            >  5:87390/1‑149 (MQ=255)
          cgcCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCttt                                                                                                                                           >  3:145710/1‑149 (MQ=255)
             cGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTccc                                                                                                                                        <  1:411419/149‑1 (MQ=255)
                     gcgTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGAc                                                                                                                                <  4:126324/149‑1 (MQ=255)
                        tataTCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTg                                                                                                                             <  7:278311/149‑1 (MQ=255)
                        tataTCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGAAAAGCGAACTTGTGATGAACAGGTTGAAGATCAATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTg                                                                                                                             >  8:182715/1‑149 (MQ=255)
                          tatCTTCCAGTTTTGCATCCCTTTAGTGCCGCTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGTGTGACGTGCTACCCCTTTCCCGATTAGACGTGc                                                                                                                            <  8:219417/148‑1 (MQ=255)
                           atCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTg                                                                                                                             >  4:375811/1‑146 (MQ=255)
                            tCTTCCAGTTTTGCATCCCTTTAGTGCCTGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTg                                                                                                                          <  1:405983/148‑1 (MQ=255)
                               tCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGAt                                                                                                                        <  2:172915/147‑1 (MQ=255)
                               tCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGAt                                                                                                                        >  1:172915/1‑147 (MQ=255)
                                ccAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGAt                                                                                                                        <  4:315999/146‑1 (MQ=255)
                                ccAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATca                                                                                                                      <  8:69709/148‑1 (MQ=255)
                                ccACTTTTGCATCCCTTTAGTGCCGGTAATTTGCGGTTCCTTAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGaa                                                                                                                        <  8:17719/146‑2 (MQ=255)
                                          aTCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTc                                                                                                           >  2:206436/1‑149 (MQ=255)
                                          aTCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTc                                                                                                           >  2:178585/1‑149 (MQ=255)
                                           tCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCg                                                                                                          >  7:184337/1‑149 (MQ=255)
                                             ccTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGc                                                                                                         <  6:97474/148‑1 (MQ=255)
                                                ttAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGAGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCttt                                                                                                     >  4:78932/1‑149 (MQ=255)
                                                ttAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGAGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCttt                                                                                                     >  4:73848/1‑149 (MQ=255)
                                                 tAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACATGTTGAAGATCCATGAGCCAAAATTGGATGTGCGGGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCttt                                                                                                     <  3:233542/148‑1 (MQ=255)
                                                 tAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCttt                                                                                                     <  4:156846/148‑1 (MQ=255)
                                                   gTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTtgat                                                                                                  <  6:87390/149‑1 (MQ=255)
                                                   gTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTtga                                                                                                   <  8:266345/148‑1 (MQ=255)
                                                   gTGCAGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTtgat                                                                                                  <  1:151012/149‑1 (MQ=255)
                                                           aaTTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACgg                                                                                          >  5:334254/1‑149 (MQ=255)
                                                            aTTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGAGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACgg                                                                                          <  3:78932/148‑1 (MQ=255)
                                                            aTTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGAGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACgg                                                                                          <  3:73848/148‑1 (MQ=255)
                                                            aTTTGCTGTGCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGACCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGt                                                                                         <  6:70033/149‑1 (MQ=255)
                                                             tttGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGt                                                                                         >  1:377093/1‑148 (MQ=255)
                                                                  tGTTCCTGAATTACCATCCGCAAAGCTAACTTGTTATGAACAGGTTGAAGATCACTGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCAGATTAGACGTGCTGATCACACCGCTGGTCGCCTTtgatga                                                                                                <  1:151748/136‑1 (MQ=255)
                                                                  tGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGGTGAAGATCCATGAGCCAAAATTGGCTGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGCTCACACCGCTGGTCGCCGTtgatga                                                                                                >  2:151748/1‑136 (MQ=255)
                                                                    ttCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCt                                                                                 >  3:277813/1‑149 (MQ=255)
                                                                    ttCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTCTGATGAGTACGGTCAGCGCCt                                                                                 <  3:141572/149‑1 (MQ=255)
                                                                         gAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGaa                                                                            >  7:392140/1‑149 (MQ=255)
                                                                          aaTTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGaa                                                                            <  7:288583/148‑1 (MQ=255)
                                                                           aTTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATg                                                                          >  5:360139/1‑149 (MQ=255)
                                                                               ccATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCg                                                                      >  3:175357/1‑149 (MQ=255)
                                                                                cATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCgg                                                                     >  6:382915/1‑149 (MQ=255)
                                                                                cATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCgg                                                                     <  7:251918/149‑1 (MQ=255)
                                                                                         aGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTa                                                            >  5:338876/1‑149 (MQ=255)
                                                                                         aGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTa                                                            <  5:112581/149‑1 (MQ=255)
                                                                                         aGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTa                                                            >  8:33430/1‑149 (MQ=255)
                                                                                             aaCTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGAt                                                        <  2:377093/149‑1 (MQ=255)
                                                                                                tGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCg                                                      >  3:324085/1‑148 (MQ=255)
                                                                                                   tgatgaACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcc                                                  <  1:135736/149‑1 (MQ=255)
                                                                                                     atgaACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGACTGGGCGGTGGTTTTTATGATCGGa                                                    <  3:179463/145‑1 (MQ=255)
                                                                                                     atgaACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGAAGATCACACAGCTCGACGAATAATAAAAAAACAATCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGAcag                                                 >  4:179463/1‑146 (MQ=255)
                                                                                                        aaCAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACa                                             <  4:277813/149‑1 (MQ=255)
                                                                                                            ggTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAAc                                         >  8:141079/1‑149 (MQ=255)
                                                                                                              ttGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGACCTACCCCTTTCCAGATTAGACGTGCTGATAACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTGTTATGATCGGACCTTACAAAACTg                                       >  7:14502/1‑149 (MQ=255)
                                                                                                                 aaGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgca                                    <  4:324085/149‑1 (MQ=255)
                                                                                                                 aaGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgc                                     <  4:175357/148‑1 (MQ=255)
                                                                                                                    aTCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGaa                                                                            >  6:33856/1‑106 (MQ=255)
                                                                                                                    aTCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGaa                                                                            <  5:33856/106‑1 (MQ=255)
                                                                                                                    aTCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGgcagca                                 >  1:230847/1‑149 (MQ=255)
                                                                                                                      ccATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCAc                                >  6:91817/1‑148 (MQ=255)
                                                                                                                       cATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACta                              <  4:283204/149‑1 (MQ=255)
                                                                                                                         tGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACtat                             >  1:9819/1‑148 (MQ=255)
                                                                                                                              cAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACg                       <  8:184337/149‑1 (MQ=255)
                                                                                                                                      gATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGt               >  7:50913/1‑149 (MQ=255)
                                                                                                                                        tgtgCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTgg             <  3:375811/149‑1 (MQ=255)
                                                                                                                                           gCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGGGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGtt          <  3:225947/149‑1 (MQ=255)
                                                                                                                                            cGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTa         <  6:360139/149‑1 (MQ=255)
                                                                                                                                              tGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACtata                            >  4:317514/1‑128 (MQ=255)
                                                                                                                                              tGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACtata                            <  3:317514/128‑1 (MQ=255)
                                                                                                                                              tGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATg       >  5:55456/1‑149 (MQ=255)
                                                                                                                                                   tGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGTTCGCCTTTGATGAGTACGGTAAGCGCCTGGGAATGGGCGGTTGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCAt  <  4:214312/149‑1 (MQ=255)
                                                                                                                                                   tGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCAt  >  7:342005/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAATTACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAATTGGATGTGCGTGACGTGCTACCCCTTTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTTATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCAT  >  NC_000913/3056404‑3056699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: