Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1600GC20.0% 59.0 / 10.5 25intergenic (‑143/+28)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (11/9);  new base C (2/3);  total (13/12)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATC  >  NC_000913/2028026‑2028306
                                                                                                                                      |                                                                                                                                                  
cTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGGGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgt                                                                                                                                      <  5:64629/149‑1 (MQ=255)
    cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGcc                                                                                                                                            <  4:52818/139‑1 (MQ=255)
    cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGcc                                                                                                                                            >  3:52818/1‑139 (MQ=255)
            ttttttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCt                                                                                                                          >  2:6018/1‑149 (MQ=255)
                       tCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGa                                                                                                                                >  8:22919/1‑132 (MQ=255)
                       tCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGAAAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGa                                                                                                                                <  7:22919/132‑1 (MQ=255)
                           gCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATCCCCTTTCACACTTCTTCCg                                                                                                           >  8:77829/1‑149 (MQ=255)
                                       gATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                                                                               >  2:161496/1‑149 (MQ=255)
                                                  gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGCCTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACACTTTCACACTTCTTCCGCAGAACGCCTCCCCTCCCCCgc                                                                                     >  2:59370/1‑147 (MQ=255)
                                                            gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                                                                            >  6:143089/1‑99 (MQ=255)
                                                            gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                                                                            <  5:143089/99‑1 (MQ=255)
                                                            gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                                                                          <  6:9583/85‑1 (MQ=39)
                                                            gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                                                                          >  5:9583/1‑85 (MQ=39)
                                                                aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttt                                                                       <  1:6018/148‑1 (MQ=255)
                                                                aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttgc                                                                      <  7:77829/149‑3 (MQ=255)
                                                                aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTc                                                                      <  1:161496/149‑1 (MQ=255)
                                                                 ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                                     >  1:46777/1‑149 (MQ=255)
                                                                   ttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                                                                    <  8:29372/148‑1 (MQ=255)
                                                                         tggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc                                                             >  1:76215/1‑149 (MQ=255)
                                                                           gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCtt                                                           <  5:89302/149‑1 (MQ=255)
                                                                                               tATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCg                                       >  1:36288/1‑149 (MQ=255)
                                                                                                    ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATa                                  >  1:2623/1‑149 (MQ=255)
                                                                                                                        acCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                                                                       >  4:8091/1‑44 (MQ=255)
                                                                                                                        acCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                                                                       <  3:8091/44‑1 (MQ=255)
                                                                                                                               cGGAGGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGa       <  1:59370/149‑1 (MQ=255)
                                                                                                                                     tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATc  <  2:36288/148‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                  
CTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTGGAGTATC  >  NC_000913/2028026‑2028306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: