Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,481	G→C	A132G (GCT→GGT)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,481	0	G	C	100.0%	199.6 / NA	57	A132G (GCT→GGT)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (27/30);  total (27/30)

GCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATAC  >  NC_000913/1261340‑1261625                                                                                                                                              |                                                                                                                                                 gcgcGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCa                                                                                                                                            <  7:77599/148‑1 (MQ=255)         acgcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTg                                                                                                                                   >  1:184567/1‑149 (MQ=255)           gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGt                                                                                                                                  >  8:102600/1‑148 (MQ=255)            cacaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCa                                                                                                                                <  4:168093/149‑1 (MQ=255)             acaACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAg                                                                                                                               >  3:157648/1‑149 (MQ=255)                aCGCCGGCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGcac                                                                                                                            <  4:178252/149‑1 (MQ=255)                  gccgccGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCacac                                                                                                                          >  1:12209/1‑149 (MQ=255)                     gccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGt                                                                                                                       <  1:82310/149‑1 (MQ=255)                                     aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                       <  7:102600/149‑1 (MQ=255)                                     aCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGga                                                                                                       <  4:157648/149‑1 (MQ=255)                                       cacaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGgaga                                                                                                     <  4:137722/149‑1 (MQ=255)                                        acaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTTTCAGCACACGGTCAACACCGACGCt                                                                                                          <  1:3919/143‑1 (MQ=255)                                        acaATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCt                                                                                                          >  2:3919/1‑143 (MQ=255)                                              gggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGt                                                                                               <  2:186782/148‑1 (MQ=255)                                               ggTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCt                                                                                             <  2:177270/149‑1 (MQ=255)                                                         aTTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACtt                                                                                   >  7:24070/1‑149 (MQ=255)                                                         aTTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGACGAAGAAAACCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCaacaaca                                                                                    >  4:134186/1‑147 (MQ=255)                                                               cTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAg                                                                             >  5:98432/1‑149 (MQ=255)                                                                 gcagcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTg                                                                           >  8:74742/1‑149 (MQ=255)                                                                    gcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                                        >  5:11223/1‑149 (MQ=255)                                                                    gcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                                        <  2:12209/149‑1 (MQ=255)                                                                    gcaTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGAtt                                                                        >  1:225593/1‑149 (MQ=255)                                                                    gcaTGTCTTACAGCAGGATCGGGCTACCAAATTCGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTTTTCACCGTGCAGATCCTCTGTCAGCACACGGTCATCACCGACTCTGGAGAGGaa                                                                                                 <  8:9746/124‑1 (MQ=255)                                                                     cacgTCTTCCAGCAGGATCGGTCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTGTGCAACCACTTTCGCAGTGATTg                                                                       <  3:134186/146‑1 (MQ=255)                                                                       tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                              <  2:105947/140‑1 (MQ=255)                                                                       tGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                              >  1:105947/1‑140 (MQ=255)                                                                                 cagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCCGCAACCACTTTCGCAGTGATTGGTACACGAGCg                                                            >  3:43499/1‑148 (MQ=255)                                                                                    gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                                        >  6:40713/1‑149 (MQ=255)                                                                                    gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                                        <  2:184567/149‑1 (MQ=255)                                                                                    gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                                        <  2:225593/149‑1 (MQ=255)                                                                                     aTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                              >  7:50258/1‑126 (MQ=255)                                                                                     aTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCa                                                                              <  8:50258/126‑1 (MQ=255)                                                                                         ggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                                                       <  2:33090/145‑1 (MQ=255)                                                                                         ggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACg                                                       >  1:33090/1‑145 (MQ=255)                                                                                                aaaTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCGGATCCACTGTCAGCACACGGTCAACACCGACGc                                                                                                           >  2:61582/1‑86 (MQ=255)                                                                                                aaaTACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCGGATCCACTGTCAGCACACGGTCAACACCGACGc                                                                                                           <  1:61582/86‑1 (MQ=255)                                                                                                     cGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCCGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTgg                                       <  4:43499/149‑1 (MQ=255)                                                                                                      gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgc                                      >  5:10549/1‑149 (MQ=255)                                                                                                       ttATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcg                                     >  3:164668/1‑149 (MQ=255)                                                                                                          tCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGgcgcg                                   >  2:122399/1‑148 (MQ=255)                                                                                                            aaCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                         <  8:149138/76‑1 (MQ=255)                                                                                                            aaCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                                         >  7:149138/1‑76 (MQ=255)                                                                                                                ggAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGc                             >  4:49647/1‑148 (MQ=255)                                                                                                                     gTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg                        >  1:104469/1‑148 (MQ=255)                                                                                                                     gTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAg                        >  7:91574/1‑148 (MQ=255)                                                                                                                       cGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg                     >  1:63609/1‑149 (MQ=255)                                                                                                                        gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGc                             >  2:78472/1‑140 (MQ=255)                                                                                                                        gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGc                             <  1:78472/140‑1 (MQ=255)                                                                                                                        gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                    <  7:74742/149‑1 (MQ=255)                                                                                                                        gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAgg                    <  4:164668/149‑1 (MQ=255)                                                                                                                         aagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg                   <  1:122399/149‑1 (MQ=255)                                                                                                                         aagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAggg                   <  3:49647/149‑1 (MQ=255)                                                                                                                            aaaCCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGAt                <  6:98432/149‑1 (MQ=255)                                                                                                                                    aaTCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCg         <  5:40713/148‑1 (MQ=255)                                                                                                                                          ttCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGa     <  6:11223/146‑1 (MQ=255)                                                                                                                                          ttCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATa   <  8:24070/148‑1 (MQ=255)                                                                                                                                          ctCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCGGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCCCGCATAGCCAAAGTAGGGGATAACAGCGGTGATAc  >  8:81356/2‑149 (MQ=255)                                                                                                                                              |                                                                                                                                                 GCGCGGGCACGCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGATAACAGCGGTGATAC  >  NC_000913/1261340‑1261625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑