New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3071417 =	47 (1.310)	4 (0.120)	4/232	4.1	9.1%	intergenic (‑173/+42)	fbaA/pgk	fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
	?	NC_000913	3071476 =	36 (1.070)					coding (1147/1164 nt)	pgk	phosphoglycerate kinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGT  >  NC_000913/3071272‑3071562                                                                                                                                                  |                                                                                                                                                  ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcg                                                                                                                                                >  3:35468/1‑149 (MQ=255)    cTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcgcgc                                                                                                                                             <  7:22781/149‑1 (MQ=255)    cTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcgcg                                                                                                                                              <  3:77163/148‑1 (MQ=255)    cTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAgcgcg                                                                                                                                              <  4:35468/148‑1 (MQ=255)               tCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAggg                                                                                                                                  >  7:81040/1‑149 (MQ=255)                    ttGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGt                                                                                                                             >  3:26377/1‑149 (MQ=255)                           tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctg                                                                                                                      >  5:4980/1‑149 (MQ=255)                            gctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgc                                                                                                                     >  1:138988/1‑149 (MQ=255)                                    tgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAt                                                                                                             >  3:159989/1‑149 (MQ=255)                                       gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAtttt                                                                                                          >  5:37640/1‑149 (MQ=255)                                       gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGCGCTTTTAGAgcacgcaat                                                                                                                                          >  6:166952/1‑114 (MQ=14)                                       gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGCGCTTTTAGAgcacgcaat                                                                                                                                          <  5:166952/117‑4 (MQ=14)                                        ttCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAttttt                                                                                                         >  7:13540/1‑149 (MQ=255)                                        ttCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGAttttt                                                                                                         >  3:31857/1‑149 (MQ=255)                                           gCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGATTCCCTGCCCTGTGATTTTTTa                                                                                                       <  7:28129/148‑1 (MQ=255)                                              cACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTActtc                                                                                                   <  8:81040/149‑1 (MQ=255)                                               aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTActtct                                                                                                  >  3:80474/1‑149 (MQ=255)                                               aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTActt                                                                                                    <  4:26377/147‑1 (MQ=255)                                                        acttacttATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGct                                                                                         >  8:145682/1‑149 (MQ=255)                                                          ttacttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGaaa                                                                                                                                        <  1:91085/100‑1 (MQ=255)                                                          ttacttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGaaa                                                                                                                                        >  2:91085/1‑100 (MQ=255)                                                            acttaTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCtt                                                                                      <  6:37640/148‑1 (MQ=255)                                                                aTGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAg                                                                                  >  2:10869/1‑148 (MQ=255)                                                                                   cACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTa                                                              >  4:72110/1‑149 (MQ=255)                                                                                      tctctTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACtt                                                           >  4:6509/1‑149 (MQ=255)                                                                                          ttGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACttt                                                          <  8:13540/146‑1 (MQ=255)                                                                                          ttGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTAcc                                                       >  2:60553/1‑149 (MQ=255)                                                                                                  ttCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGt                                                               >  4:101980/1‑133 (MQ=255)                                                                                                  ttCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGt                                                               <  3:101980/133‑1 (MQ=255)                                                                                                        cAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTc                                           <  3:6509/147‑1 (MQ=255)                                                                                                        cAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGa                                         <  6:4980/149‑1 (MQ=255)                                                                                                         aaCTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAg                                        >  7:90021/1‑149 (MQ=255)                                                                                                          aCTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAgg                                       <  1:60553/149‑1 (MQ=255)                                                                                                             cGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAAt                                    <  8:90021/149‑1 (MQ=255)                                                                                                                 ttattTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGc                                                                            <  1:186573/105‑1 (MQ=255)                                                                                                                 ttattTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGc                                                                            >  2:186573/1‑105 (MQ=255)                                                                                                                      tAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGGTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGcgccgcc                            <  4:31857/148‑1 (MQ=255)                                                                                                                          gTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGTTTTCACTGCCCTGTGATTTTTTACTTCTTAGCTCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACATTCAACGAATTCGAGTAATTAGCCGCCGCCAg                       >  4:113846/1‑149 (MQ=255)                                                                                                                               aaaaaTTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgc                                                                                                                     >  4:41285/1‑50 (MQ=255)                                                                                                                               aaaaaTTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTccctgc                                                                                                                     <  3:41285/50‑1 (MQ=255)                                                                                                                                 aaaTTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGAt                <  7:145682/149‑1 (MQ=255)                                                                                                                                      gCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAg            <  2:138988/148‑1 (MQ=255)                                                                                                                                      gCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAg            <  3:72110/148‑1 (MQ=255)                                                                                                                                               aaaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGt  >  6:69632/1‑149 (MQ=255)                                                                                                                                                aaaGCGCGCTGAAACAAGGTCAGGTTTCCCTTCCCTGTGATTTTTTACTTCTTAGCGCGCTATTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGACAGTGGAGATGTAGGAGAtttt    <  3:113846/146‑1 (MQ=255)                                                                                                                                                aaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGAtttt    <  4:80474/146‑1 (MQ=255)                                                                                                                                                aaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGt  <  1:10869/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATGAAAAATTGCGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGT  >  NC_000913/3071272‑3071562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.