Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,261,481	G→C	A132G (GCT→GGT)	prs ←	phosphoribosylpyrophosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,261,481	0	G	C	100.0%	130.4 / NA	37	A132G (GCT→GGT)	prs	phosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/19);  total (18/19)

GCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGA  >  NC_000913/1261350‑1261610                                                                                                                                    |                                                                                                                                  gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACt                                                                                                                     <  7:21002/146‑1 (MQ=255) gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACt                                                                                                                     >  8:21002/1‑146 (MQ=255) gcacAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTc                                                                                                                  <  1:111079/149‑1 (MQ=255)         ccgccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACg                                                                                                          <  7:22013/149‑1 (MQ=255)          cgccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCCTGTCTGCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGACCGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCCCTGTCAGCACACgg                                                                                                         >  3:37708/1‑149 (MQ=255)          cgccgATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACgg                                                                                                         <  4:78533/149‑1 (MQ=255)                     ggAGAAACCACCATTGGGGTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGTGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGGCAGCACACGGTCAACACCGAc                                                                                              <  6:48549/149‑1 (MQ=255)                         aaaCCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                          >  7:112328/1‑149 (MQ=255)                            ccACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTg                                                                                          >  1:130526/1‑146 (MQ=255)                                  ttGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAg                                                                                 <  2:121597/149‑1 (MQ=255)                                            cAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGATCAACACCGACGCTGGAGAGGAAGTCTGCAACCa                                                                       >  1:66478/1‑149 (MQ=255)                                                            aTGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTgg                                                       >  1:43618/1‑149 (MQ=255)                                                               tCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTa                                                     >  6:167380/1‑148 (MQ=255)                                                                   ccAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                  <  2:43618/147‑1 (MQ=255)                                                                    cagcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAg                                               >  1:97502/1‑149 (MQ=255)                                                                     agcagGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGc                                              <  8:112328/149‑1 (MQ=255)                                                                          gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                         >  7:34183/1‑149 (MQ=255)                                                                          gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                         >  4:102284/1‑149 (MQ=255)                                                                          gATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGCTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAc                                         <  5:167380/149‑1 (MQ=255)                                                                           aTCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAAca                                        <  8:34183/149‑2 (MQ=255)                                                                              gggCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAc                                     >  3:38401/1‑149 (MQ=255)                                                                                gCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGAcgc                                   <  3:102284/149‑1 (MQ=255)                                                                                    ccAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGAc                                <  2:97502/148‑1 (MQ=255)                                                                                    ccAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACg                               >  1:172093/1‑149 (MQ=255)                                                                                            gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                  <  5:154030/122‑1 (MQ=255)                                                                                            gTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTacac                                                  >  6:154030/1‑122 (MQ=255)                                                                                                  aaCCGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGGCTGCAACCACTTTCGCAGTGAGTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCa                  <  4:37708/148‑1 (MQ=255)                                                                                                    ccGGAACGTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAg               >  4:43952/1‑149 (MQ=255)                                                                                                           gTCGAAGAAACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGt        <  3:43952/149‑1 (MQ=255)                                                                                                              gaagaaACCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAg      <  4:38401/148‑1 (MQ=255)                                                                                                                  aaaCCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGa  >  1:179785/1‑148 (MQ=255)                                                                                                                    aCCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTa       <  2:97088/141‑1 (MQ=255)                                                                                                                    aCCCTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTa       >  1:97088/1‑141 (MQ=255)                                                                                                                      ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGa                                                                                               >  1:106547/1‑51 (MQ=255)                                                                                                                      ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGa                                                                                               <  2:106547/51‑1 (MQ=255)                                                                                                                      ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGt                                                             <  5:85577/85‑1 (MQ=255)                                                                                                                      ccTGAATCTGTTCACCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGt                                                             >  6:85577/1‑85 (MQ=255)                                                                                                                                    |                                                                                                                                  GCACAACGCCGCCGATGTCCGGAGAAACCACAATTGGGTTATCCAGATTCAGCTGCAGCATGTCTTCCAGCAGGATCGGGCTACCAAATACGTTATCAACCGGAACGTCGAAGAAACCCTGAATCTGTTCAGCGTGCAGATCCACTGTCAGCACACGGTCAACACCGACGCTGGAGAGGAAGTCTGCAACCACTTTCGCAGTGATTGGTACACGAGCGGAACGGACGCGACGGTCCTGGCGCGCATAGCCAAAGTAGGGGA  >  NC_000913/1261350‑1261610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑