| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,644,332 | T→A | intergenic (‑28/+319) | ydfU ← / ← rem | Qin prophage; uncharacterized protein/Qin prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,644,332 | 0 | T | A | 98.5% | 223.4 / ‑4.2 | 65 | intergenic (‑28/+319) | ydfU/rem | Qin prophage; uncharacterized protein/Qin prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base A (35/29); total (36/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
GTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGCCTGTTCTGCAGTG > NC_000913/1644190‑1644474 | gttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCggg < 7:23853/148‑1 (MQ=255) ttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGAc > 5:138154/1‑149 (MQ=255) ttccggttccACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGAc > 4:3753/1‑149 (MQ=255) aTTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATaa < 6:138154/149‑1 (MQ=255) aaaTATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGc < 3:1329/149‑1 (MQ=255) tGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCg > 5:59586/1‑108 (MQ=255) tGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCg < 6:59586/108‑1 (MQ=255) gTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACa > 3:77661/1‑147 (MQ=255) tATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACa > 7:54476/1‑146 (MQ=255) tGGATTCACGGCCCGGCTTAAGGACCACCAGCCCACGCTCAGGCACCAGACCAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAgaga < 7:46707/148‑1 (MQ=255) aTTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTAt > 6:22832/1‑149 (MQ=255) ttAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCg > 8:12880/1‑109 (MQ=255) ttAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCg < 7:12880/109‑1 (MQ=255) aGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCga < 3:3753/149‑1 (MQ=255) ccaGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAg > 1:65594/1‑110 (MQ=255) ccaGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAg < 2:65594/110‑1 (MQ=255) cccAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACgtg < 3:72856/84‑1 (MQ=255) cccAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACgtg > 4:72856/1‑84 (MQ=255) cccAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCt > 5:52732/1‑149 (MQ=255) tCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTgag > 1:79346/1‑138 (MQ=255) tCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTgag < 2:79346/138‑1 (MQ=255) tCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTaa > 5:56061/1‑149 (MQ=255) aGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATaa < 5:100427/92‑1 (MQ=255) aGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATaa > 6:100427/1‑92 (MQ=255) gAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACt > 2:197160/1‑125 (MQ=255) gAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACt < 1:197160/125‑1 (MQ=255) aaCAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGaa > 5:90378/1‑145 (MQ=255) aaCAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGaa < 6:90378/145‑1 (MQ=255) aaCAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCcg > 5:65815/1‑149 (MQ=255) aaCAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCcg > 5:171039/1‑149 (MQ=255) aCAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCcgc < 6:171039/149‑1 (MQ=255) gTCTCAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGAACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGt < 3:12524/112‑1 (MQ=255) gTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGt > 4:12524/1‑112 (MQ=255) gTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTgaga < 4:68668/123‑1 (MQ=255) gTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTgaga > 3:68668/1‑123 (MQ=255) gTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAAc > 3:25381/1‑134 (MQ=255) gTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAAc < 4:25381/134‑1 (MQ=255) tAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGc < 4:74373/64‑1 (MQ=255) tAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGc > 3:74373/1‑64 (MQ=255) tAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGa < 3:133175/85‑1 (MQ=255) tAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGa > 4:133175/1‑85 (MQ=255) tAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAAc > 8:98348/1‑131 (MQ=255) tAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAAc < 7:98348/131‑1 (MQ=255) aaGTAATACCCGCACGTTACCTCCAGCTCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGCATACGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTGAACCTCGAACCCGCGCCTGCgg > 7:123894/1‑149 (MQ=255) aaGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCgg > 5:713/1‑149 (MQ=255) aGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGGAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCg < 8:54476/147‑1 (MQ=255) aGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCg < 4:77661/147‑1 (MQ=255) gTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCa < 8:128728/95‑1 (MQ=255) gTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCa > 7:128728/1‑95 (MQ=255) aTACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGcc < 3:57458/76‑1 (MQ=255) aTACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGcc > 4:57458/1‑76 (MQ=255) aTACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAg > 5:51277/1‑148 (MQ=255) ccGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGCCGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGCACCCGCGCCTGCGGTAAgaatg > 8:87244/1‑148 (MQ=255) ccGCACGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAgaatg > 6:2427/1‑148 (MQ=255) aCGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATc < 2:3684/148‑1 (MQ=255) aCGTTACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATc > 1:3684/1‑148 (MQ=255) ttACCTCCAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGc > 6:8826/1‑148 (MQ=255) cAGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGc < 5:2427/149‑1 (MQ=255) aGATCCGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGcc > 8:13528/1‑149 (MQ=255) cGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAAc > 2:189349/1‑108 (MQ=255) cGTTGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAAc < 1:189349/108‑1 (MQ=255) ttGCTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGCCTGTTCTg < 6:51277/149‑1 (MQ=255) cTGGAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGCCTGTTCTGCa < 6:56061/148‑1 (MQ=255) ggAAAGTGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGCCTGTTCTGCAGTg > 6:2590/1‑149 (MQ=255) gAAAGAGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGCCTGTTCTGCAGTg > 4:135451/1‑148 (MQ=255) | GTTCCGGTTCCACCAGCACTCGAGGATTATGAAATATCTGTATGGATTCACGGCCCGGCTTAAGGACCACCAGCCCAAGCTCAGGCACCAGAACAGGTCTAAGTAATACCCGCACGTTACCTCCAGATCCGTTGCTGGAAAGTGCGGGACGCACGTGGTGGGCGTTCGGAATAAGGCAGCCTGACAGAGATTATCCAGTGCCGATAGTCGAGACTGAGAGCTTTCTTAACCTCGAACCCGCGCCTGCGGTAAGAATGAATCAGCCATTCGGCCTGTTCTGCAGTG > NC_000913/1644190‑1644474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |