New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4406040 =	37 (1.020)	6 (0.180)	6/222	2.3	16.0%	intergenic (+55/‑150)	purA/nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
	?	NC_000913	4406075 =	29 (0.870)					intergenic (+90/‑115)	purA/nsrR	adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
Rejected: Frequency below/above cutoff threshold.

TACCGGTCCGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATT  >  NC_000913/4405931‑4406221                                                                                                                                                 |                                                                                                                                                   tACCGGTCCGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTTGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTCAGCCATTACGCTATCCGACACAgtgt                                                                                                                                                  <  3:9040/147‑1 (MQ=255) tACCGGTCCGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGACGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGtt                                                                                                                                                 >  3:61247/1‑148 (MQ=255)  aCCGGTCCGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTa                                                                                                                                                >  7:163348/1‑148 (MQ=255)  aCCGGTCCGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCGGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTACGCCATTACGCTATCCGACACAGTGTTa                                                                                                                                                >  7:161146/1‑148 (MQ=255)         cGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGGCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTc                                                                                                                                         <  4:61247/148‑1 (MQ=255)                  cTGAAACCATGATTCTGCGCGACCCGTTCGCCGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTttcc                                                                                                                               >  5:117821/1‑149 (MQ=255)                                 tGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGaaa                                                                                                                >  4:117211/1‑149 (MQ=255)                                       aaccGGTCGCCGCGTAATTCTGGTACGCCTGGCATATATTTTTCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAAtt                                                                                                          <  8:161146/147‑1 (MQ=255)                                       aCCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGGGATACATTGCTGGGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAAtt                                                                                                          <  8:163348/149‑1 (MQ=255)                                               cagccacaagctgtgtcgctaatttatttcagttcggggaaggaaaaaagcgaggatttaacaCTGTGTCGGGTAAGCCATTACGCTATCCGACACAgt                                                                                                                                                    <  1:56546‑M1/36‑1 (MQ=255)                                               cagccacaagctgtgtcgctaatttatttcagttcggggaaggaaaaaagcgaggatttaacaCTGTGTCGGGTAAGCCATTACGCTATCCGACACAgt                                                                                                                                                    >  2:56546‑M1/64‑99 (MQ=255)                                                    gTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGc                                                                                                       <  8:62133/139‑1 (MQ=255)                                                    gTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGc                                                                                                       >  7:62133/1‑139 (MQ=255)                                                               aCGCCTGGCAGATATTTTGCCTGCCGGGTGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGtt                                                                                   >  3:109749/1‑148 (MQ=255)                                                                 gCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTat                                                                                >  7:15066/1‑149 (MQ=255)                                                                        aGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCaa                                                                          >  7:3973/1‑148 (MQ=255)                                                                          atatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAatat                                                                       <  8:3973/149‑1 (MQ=255)                                                                          atatTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAata                                                                        >  8:16299/1‑148 (MQ=255)                                                                                                  tgtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAtt                                                   <  7:84790/145‑1 (MQ=255)                                                                                                  tgtgATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGAtt                                                   >  8:84790/1‑145 (MQ=255)                                                                                                          aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                       >  3:54464/1‑149 (MQ=255)                                                                                                          aTTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAgg                                       <  6:117821/149‑1 (MQ=255)                                                                                                             actgtgtcggatagcgtaatggcttacccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTg                                                                                      <  1:56546‑M2/63‑1 (MQ=255)                                                                                                             actgtgtcggatagcgtaatggcttacccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTg                                                                                      >  2:56546‑M2/37‑99 (MQ=255)                                                                                                              ctgtgtcggatagcgtaatggcttacccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATaa                                                                     <  8:3093‑M2/80‑1 (MQ=255)                                                                                                              ctgtgtcggatagcgtaatggcttacccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATaa                                                                     >  7:3093‑M2/36‑115 (MQ=255)                                                                                                               TGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                  >  4:109749‑M1/1‑149 (MQ=255)                                                                                                               TGTCTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCTCTTTTTTCCTACCCCGAACTGAAATAAATTAGCGACACAGAATGTGGCTGGTTTATCATCAATATAAATCTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                  <  8:130148‑M1/149‑1 (MQ=255)                                                                                                                 TGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAtgt                                >  7:16299‑M1/1‑149 (MQ=255)                                                                                                                  gtcggatagcgtaatggcttacccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                  >  1:34056‑M2/32‑146 (MQ=255)                                                                                                                  gtcggatagcgtaatggcttacccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGAt                                  <  2:34056‑M2/115‑1 (MQ=255)                                                                                                                        tAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGt                                                                                    >  1:209542/1‑90 (MQ=255)                                                                                                                        tAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGt                                                                                    <  2:209542/90‑1 (MQ=255)                                                                                                                          aGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCACGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAAc                       <  3:117211/149‑1 (MQ=255)                                                                                                                           gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGa                                                     >  1:174862/1‑118 (MQ=255)                                                                                                                           gCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGa                                                     <  2:174862/118‑1 (MQ=255)                                                                                                                             cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                    <  8:15066/149‑1 (MQ=255)                                                                                                                             cATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAg                    <  4:54464/149‑1 (MQ=255)                                                                                                                                     tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACAGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                           <  2:88005/118‑1 (MQ=255)                                                                                                                                     tATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACAGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCtttt                                           >  1:88005/1‑118 (MQ=255)                                                                                                                                        ccgacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGAt          >  6:18210‑M2/10‑148 (MQ=255)                                                                                                                                          gacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                         >  3:160077‑M2/8‑115 (MQ=255)                                                                                                                                          gacacagTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGa                                         <  4:160077‑M2/108‑1 (MQ=255)                                                                                                                                               agTGTTAAATGCTTGGTTTTTTGCTTCCCCGTACTGAAATAAATTAGGGACACAGCTTGTGGTTGGTTTATGATCAATATAAATGTATTTTTTCCCGATTTGCGTTTTGTGGTTGTTGTGCAGTTAACGAGTTTCACTGATTACGGAtt  <  7:130148‑M2/147‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TACCGGTCCGGATCGTACTGAAACCATGATTCTGCGCGACCCGTTCGACGCGTAATTCTGGTACGCCTGGCAGATATTTTGCCTGCCGGGCGAACAGTGTGATACATTGCTGTGTCGGGTAAGCCATTACGCTATCCGACACAGTGTTAAATCCTCGCTTTTTTCCTTCCCCGAACTGAAATAAATTAGCGACACAGCTTGTGGCTGGTTTATCATCAATATAAATGTATTTTTTCCCGATTTCCCTTTTGAGGTTGATGTGCAGTTAACGAGTTTCACTGATTACGGATT  >  NC_000913/4405931‑4406221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.