Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,002,530 G→A G729D (GGT→GAT)  xdhA → xanthine dehydrogenase, molybdenum binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,002,5300GA98.8% 292.2 / ‑4.4 85G729D (GGT→GAT) xdhAxanthine dehydrogenase, molybdenum binding subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (42/42);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGGC  >  NC_000913/3002393‑3002675
                                                                                                                                         |                                                                                                                                                 
gCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatc                                                                                                                                         >  3:107646/1‑148 (MQ=255)
 ccGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaa                                                                                                                                       <  4:107646/149‑1 (MQ=255)
 ccGGAGCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGcaatcaa                                                                                                                                       <  3:36382/149‑1 (MQ=255)
          ccACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGcc                                                                                                                              >  5:262153/1‑149 (MQ=255)
               aCTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGa                                                                                                                         <  7:116075/149‑1 (MQ=255)
                 tGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACg                                                                                                                       >  3:40364/1‑149 (MQ=255)
                   gAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGTAGATGGCTACCGATGTTGCGATCAATACACTGCCGCTGACg                                                                                                                       <  2:266703/147‑1 (MQ=255)
                   gAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGc                                                                                                                      <  2:44217/148‑1 (MQ=255)
                       gcgcGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                 <  5:210993/149‑1 (MQ=255)
                        cgcgTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGTGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGATGACGCCAAAAc                                                                                                                >  1:352401/1‑149 (MQ=255)
                        cgcgTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAAc                                                                                                                <  8:194391/149‑1 (MQ=255)
                         gcgTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACg                                                                                                               >  6:324610/1‑149 (MQ=255)
                           gTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATATTTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGt                                                                                                             <  1:53141/149‑1 (MQ=255)
                           gTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGt                                                                                                             >  5:5218/1‑149 (MQ=255)
                              cgtcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTta                                                                                                           <  4:369607/148‑1 (MQ=255)
                               gtcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTtat                                                                                                          <  2:185608/148‑1 (MQ=255)
                                tcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTatat                                                                                                        <  1:225968/149‑1 (MQ=255)
                                tcgAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTatat                                                                                                        <  8:319123/149‑1 (MQ=255)
                                   aaaTCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGaa                                                                                                     <  3:46645/149‑1 (MQ=255)
                                      tCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACg                                                                                                               >  8:357905/1‑136 (MQ=255)
                                      tCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACg                                                                                                               <  7:357905/136‑1 (MQ=255)
                                        aaTGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                 <  3:346515/132‑1 (MQ=255)
                                        aaTGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                 >  4:346515/1‑132 (MQ=255)
                                          tGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTc                                                                                               >  8:116300/1‑148 (MQ=255)
                                                  aaTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                 >  7:204027/1‑122 (MQ=255)
                                                  aaTCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCaaaa                                                                                                                 <  8:204027/122‑1 (MQ=255)
                                                    tCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAg                                                                                     >  1:173841/1‑148 (MQ=255)
                                                       gCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgatt                                                                                 >  7:236733/1‑149 (MQ=255)
                                                       gCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgatt                                                                                 >  1:208295/1‑149 (MQ=255)
                                                       gCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgatt                                                                                 <  8:236733/149‑1 (MQ=255)
                                                          tACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgat                                                                              >  7:398532/1‑149 (MQ=255)
                                                          tACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgat                                                                              <  8:398532/149‑1 (MQ=255)
                                                            cGGACATAAGTCACTGGGGGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCTTAACGCGGTGAATATGGCTACCTATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGAttt                                                                            <  4:277351/149‑1 (MQ=255)
                                                             ggACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAagag                                                                                                  <  8:105729/126‑1 (MQ=255)
                                                             ggACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAagag                                                                                                  >  7:105729/1‑126 (MQ=255)
                                                             ggACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGAttt                                                                            >  5:323141/1‑148 (MQ=255)
                                                              gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGa                                                                          <  2:139744/149‑1 (MQ=255)
                                                              gACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGa                                                                          <  6:323141/149‑1 (MQ=255)
                                                               aCATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATTCACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattga                                                                               >  3:271787/1‑143 (MQ=255)
                                                                  tAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgat                                                                              <  6:162968/141‑1 (MQ=255)
                                                                  tAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGgattgat                                                                              >  5:162968/1‑141 (MQ=255)
                                                                  tAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGa                                                                       >  5:18456/1‑148 (MQ=255)
                                                                      tCACTGGGTGAGCCAGCAATAATACCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATGTGAGGATAAca                                                                  >  8:290601/1‑149 (MQ=255)
                                                                      tCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAca                                                                  <  6:262153/149‑1 (MQ=255)
                                                                        aCTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGGGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACTGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAAcatc                                                                <  4:355424/149‑1 (MQ=255)
                                                                             gTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGtt                                                           <  6:5218/149‑1 (MQ=255)
                                                                              tGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGttt                                                          >  3:18880/1‑149 (MQ=255)
                                                                                   caccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGATGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAttt                                                     >  4:316636/1‑149 (MQ=255)
                                                                                    accaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAtttt                                                    >  1:27689/1‑149 (MQ=255)
                                                                                    accaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAtttt                                                    >  1:224633/1‑149 (MQ=255)
                                                                                    accaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGAtttt                                                    >  5:173112/1‑149 (MQ=255)
                                                                                     ccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACt                                                                                                                                 >  2:156800/1‑71 (MQ=255)
                                                                                     ccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTg                                                   >  3:25241/1‑149 (MQ=255)
                                                                                     ccaATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTg                                                   >  3:85629/1‑149 (MQ=255)
                                                                                      caATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGc                                                  >  1:336576/1‑149 (MQ=255)
                                                                                        ataatTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGctt                                                <  2:27689/149‑1 (MQ=255)
                                                                                         taatTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGcttc                                               >  6:38576/1‑149 (MQ=255)
                                                                                         taatTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGcttc                                               >  8:79350/1‑149 (MQ=255)
                                                                                            ttCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTa                                            >  4:310706/1‑149 (MQ=255)
                                                                                               cTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCa                                         >  3:158230/1‑149 (MQ=255)
                                                                                                  ttGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATc                                       <  1:337632/148‑1 (MQ=255)
                                                                                                     ccGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATcgcg                                    >  4:351414/1‑148 (MQ=255)
                                                                                                      cGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgca                                  <  2:336576/149‑1 (MQ=255)
                                                                                                       gctgctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGTCACGATAGATTTGAGGATAACATAATGTTTGATTTTGCTTCTTACCATCGCgcag                                 >  3:206151/1‑149 (MQ=255)
                                                                                                       gctgctATTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCgcag                                 >  2:379040/1‑149 (MQ=255)
                                                                                                             aTTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTAc                                           >  2:271905/1‑133 (MQ=255)
                                                                                                             aTTCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTAc                                           <  1:271905/133‑1 (MQ=255)
                                                                                                              ttCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAAcc                            <  5:38576/147‑1 (MQ=255)
                                                                                                              ttCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAAcc                            <  7:79350/147‑1 (MQ=255)
                                                                                                              ttCGTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCt                          >  2:255042/1‑149 (MQ=255)
                                                                                                                 gTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGga                                                                                   >  2:389837/1‑89 (MQ=255)
                                                                                                                 gTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGga                                                                                   <  1:389837/89‑1 (MQ=255)
                                                                                                                 gTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGc                       >  1:368233/1‑149 (MQ=255)
                                                                                                                 gTAACGCGGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACACCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGc                       <  2:173841/149‑1 (MQ=255)
                                                                                                                   aaCGCGGTGAAGATGGCTACCGATGTTGCAATCAATAGACTGCCCCTGACGCCAAACCGGTAAGATGAACAGTTCCATCTGGCAGGATTGATTTGAGTATAACAACATGTTTGATTTTGCTTATTACCATCCCGCAGAAACCCTTGcc                      >  2:129203/1‑148 (MQ=255)
                                                                                                                       cgGTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGc                 <  4:18880/149‑1 (MQ=255)
                                                                                                                        ggTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAAc                             <  5:372079/136‑1 (MQ=255)
                                                                                                                        ggTGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAAc                             >  6:372079/1‑136 (MQ=255)
                                                                                                                          tGAAGATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCAt              <  1:379040/149‑1 (MQ=255)
                                                                                                                              gATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAAc          <  4:158230/149‑1 (MQ=255)
                                                                                                                              gATGGCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAAc          <  1:255042/149‑1 (MQ=255)
                                                                                                                                  gCTACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACctg       <  7:116300/148‑1 (MQ=255)
                                                                                                                                    tACCGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCACCCCTTGCCGATGCCATCAACctgctg    >  2:373525/1‑149 (MQ=255)
                                                                                                                                      ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTgctggc  <  4:25241/149‑1 (MQ=255)
                                                                                                                                      ccGATGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTgctggc  <  6:173112/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                 
GCCGGATCTGCCACAACTGGAAAGCGCGTTCGTCGAAATCAATGAGCCGCAATCCGCATACGGACATAAGTCACTGGGTGAGCCACCAATAATTCCTGTTGCCGCTGCTATTCGTAACGCGGTGAAGATGGCTACCGGTGTTGCAATCAATACACTGCCGCTGACGCCAAAACGGTTATATGAAGAGTTCCATCTGGCAGGATTGATTTGAGGATAACATCATGTTTGATTTTGCTTCTTACCATCGCGCAGCAACCCTTGCCGATGCCATCAACCTGCTGGC  >  NC_000913/3002393‑3002675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: